LOCUS       sequence-226664-        6087 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Nuclear Pore, Excitation: 530, Emission: 541.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6087)
  AUTHORS   Hoi H, Howe ES, Ding Y, Zhang W, Baird MA, Sell BR, Allen JR, 
            Davidson MW, Campbell RE
  TITLE     An engineered monomeric Zoanthus sp. yellow fluorescent protein.
  JOURNAL   Chem Biol. 2013 Oct 24;20(10):1296-304. doi: 
            10.1016/j.chembiol.2013.08.008. Epub 2013 Oct 3.
  PUBMED    24094838
REFERENCE   2  (bases 1 to 6087)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2013.08.008"; journalName: "Chem Biol"; date: 
            "2013-10-24- 24"; volume: "20"; issue: "10"; pages: "1296-304"
COMMENT     Sequence Label: mPapaya-Nup50-N-10
FEATURES             Location/Qualifiers
     source          1..6087
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      540..549
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1974..1983
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1980..2693
                     /codon_start=1
                     /product="monomeric yellow fluorescent protein derived from
                     the Zoanthus fluorescent protein zFP538"
                     /label=mPapaya1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEGQSKHGLKEEMTVKYHMEGCVNGHKFVITGEGIGNPFKG
                     KQTANLCVIEGGPLPFSEDILSPGFKYGDRIFTEYPQDIVDYFKNSCPAGYTWERSFLF
                     EDGAVCRCNVDITVSEKENCIYHKSIFRGVNFPADGPVMKKMTTNWEASTEKIVPVPKQ
                     GILKGKVKMYLLLKDGGRYHCQFDTVYKAKSVPSKMPEWHFIQHKLLREDRSDAKNQKW
                     QLTEHAIAGMDELYK"
     polyA_signal    2815..2936
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2852..2871)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2906..2925
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2943..3398)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3080..3101)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3292..3311
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3425..3529
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3531..3888
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3531..3722
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3554..3574)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3739..3874
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3923..4717
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3923..4717
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3977..3996)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4587..4606
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4905..4924)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4949..4996
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5325..5913
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5814..5833
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgatg
      541 ccaccatggc caaaagaaat gccgagaagg aactgacaga taggaattgg gatcaagaag
      601 atgaagctga agaggtggga acattctcca tggccagtga ggaagtcttg aagaatagag
      661 ccataaagaa agcaaagcgc agaaatgttg gatttgaatc tgacactgga ggagccttta
      721 aaggttttaa aggtttggtg gtaccttctg gaggaggacg cttttctgga tttggtagtg
      781 gcgctggagg gaagcctctg gaaggactgt cgaatggaaa caacataacc agtgcccctc
      841 ccttcgccag tgcaaaggca gcggcagatc ccaaggtagc ctttggttct cttgctgcaa
      901 atggccctac caccttggtt gataaagttt caaatcccaa aactaatggg gacagtcagc
      961 agccctcctc ctctggcctt gcttccagta aagcttgtgt cggaaatgcc tatcacaagc
     1021 agttggccgc cttgaactgc tccgtgcggg attggatagt gaagcacgtg aatacaaacc
     1081 ccctctgtga tctgacacct atctttaaag actatgagaa atatttagca aacattgaac
     1141 agcaacacgg gaacagtggc aggaattctg aaagtgaatc taacaaagtg gcagctgaaa
     1201 cacagtctcc ttcccttttt ggctcaacaa aattacagca agagtcaacg tttttgtttc
     1261 atggcaacaa aactgaagat acacctgaca agaagatgga ggtggcatct gaaaagaaaa
     1321 cggacccatc atcactagga gcgacaagtg cctcatttaa tttcggcaag aaagttgata
     1381 gctctgtttt gggctcatta agctctgtcc ccctgactgg attttctttc tcccctggaa
     1441 actccagttt atttggcaaa gatactaccc agagtaaacc agtctcttca ccatttccca
     1501 ctaaaccatt ggagggccaa gcagaaggtg acagtggtga atgcaaaggt ggagatgaag
     1561 aagagaatga tgagccaccc aaagtagtag ttaccgaagt aaaagaagaa gacgcttttt
     1621 actccaaaaa gtgtaaactg ttttacaaga aagacaatga gtttaaagag aaaggcatag
     1681 gtactctgca tttaaaacct acagcaaatc agaagacaca gcttttggtg cgggcagaca
     1741 ccaatttagg caacatattg ctgaacgttc tgattccacc caatatgcca tgtacgcgaa
     1801 cagggaagaa taacgttctt atcgtctgtg ttccaaatcc accaattgac gagaagaatg
     1861 ccaccatgcc agtcaccatg ttgattcggg taaaaaccag cgaggatgca gacgagttgc
     1921 acaaaatttt actggagaaa aaggatgcca gctccggagg ggatccaccg gtcgccacca
     1981 tggtgagcaa gggcgagggg caatcgaagc atgggctaaa ggaggagatg acggtgaagt
     2041 accacatgga aggttgcgtt aatggtcaca aatttgtcat tactggagag ggcattggaa
     2101 acccttttaa gggtaagcaa actgcaaatt tgtgtgtgat agaaggaggc ccgctgccgt
     2161 tctcggagga cattttaagt ccgggtttta aatatggtga ccggattttc acagagtacc
     2221 cacaggatat tgtagattac ttcaagaact catgtccagc gggctatacg tgggaaagga
     2281 gcttcctctt tgaggacgga gcggtctgtc gatgcaacgt ggacataaca gtctctgaaa
     2341 aggagaactg catctatcac aaaagtatct tcagaggggt gaattttccc gccgacggcc
     2401 ccgtaatgaa gaaaatgacc actaattggg aagctagtac cgagaaaatt gtgcctgttc
     2461 caaagcaagg gatattaaaa ggaaaggtca aaatgtacct attgctgaag gatggcggtc
     2521 gttatcattg ccagtttgat acggtatata aagctaaatc agtgccctcc aagatgccag
     2581 aatggcattt tatacagcat aagctcctta gggaggaccg ctctgatgct aaaaaccaga
     2641 aatggcaatt aacagaacat gcaatcgcag gcatggacga gctgtacaag taagcggccg
     2701 cgactctaga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
     2761 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
     2821 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     2881 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaaggc
     2941 gtaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
     3001 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
     3061 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
     3121 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
     3181 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
     3241 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
     3301 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
     3361 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa
     3421 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     3481 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagtc ctgaggcgga
     3541 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     3601 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     3661 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     3721 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     3781 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     3841 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaga tcgatcaaga
     3901 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc
     3961 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
     4021 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
     4081 gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac
     4141 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
     4201 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
     4261 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
     4321 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
     4381 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
     4441 gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
     4501 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
     4561 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
     4621 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
     4681 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg
     4741 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat
     4801 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     4861 gatctcatgc tggagttctt cgcccaccct agggggaggc taactgaaac acggaaggag
     4921 acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggt
     4981 gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc
     5041 ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc
     5101 ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata
     5161 gcctcaggtt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     5221 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     5281 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     5341 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     5401 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     5461 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     5521 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     5581 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     5641 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     5701 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     5761 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     5821 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     5881 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     5941 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     6001 gtggataacc gtattaccgc catgcattag ttattaatag taatcaatta cggggtcatt
     6061 agttcatagc ccatatatgg agttccg
//
