LOCUS       sequence-226666-        6038 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Gap Junctions, Excitation: 530, Emission: 541.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6038)
  AUTHORS   Hoi H, Howe ES, Ding Y, Zhang W, Baird MA, Sell BR, Allen JR, 
            Davidson MW, Campbell RE
  TITLE     An engineered monomeric Zoanthus sp. yellow fluorescent protein.
  JOURNAL   Chem Biol. 2013 Oct 24;20(10):1296-304. doi: 
            10.1016/j.chembiol.2013.08.008. Epub 2013 Oct 3.
  PUBMED    24094838
REFERENCE   2  (bases 1 to 6038)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2013.08.008"; journalName: "Chem Biol"; date: 
            "2013-10-24- 24"; volume: "20"; issue: "10"; pages: "1296-304"
COMMENT     Sequence Label: mPapaya-Cx43-7
FEATURES             Location/Qualifiers
     source          1..6038
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      1925..1934
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1931..2644
                     /codon_start=1
                     /product="monomeric yellow fluorescent protein derived from
                     the Zoanthus fluorescent protein zFP538"
                     /label=mPapaya1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEGQSKHGLKEEMTVKYHMEGCVNGHKFVITGEGIGNPFKG
                     KQTANLCVIEGGPLPFSEDILSPGFKYGDRIFTEYPQDIVDYFKNSCPAGYTWERSFLF
                     EDGAVCRCNVDITVSEKENCIYHKSIFRGVNFPADGPVMKKMTTNWEASTEKIVPVPKQ
                     GILKGKVKMYLLLKDGGRYHCQFDTVYKAKSVPSKMPEWHFIQHKLLREDRSDAKNQKW
                     QLTEHAIAGMDELYK"
     polyA_signal    2766..2887
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2803..2822)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2857..2876
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2894..3349)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3031..3052)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3243..3262
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3376..3480
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3482..3839
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3482..3673
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3505..3525)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3690..3825
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3874..4668
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3874..4668
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3928..3947)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4538..4557
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4856..4875)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4900..4947
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5276..5864
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5765..5784
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggactcag atctcgagct caagcttcga attcggcagc acttttcttt cattgggggg
      601 aaggcgtgag gaaagtacca aacagcagca gacttttaaa ctttaaacag acaggtctga
      661 gagcctgaac tctcattttt cctttgactt cagcctccaa ggagttccac caactttggc
      721 gcgccggctt cactttcatt aagtgaaaga gaggtgccca gacatgggtg actggagtgc
      781 cttggggaag cttctggaca aggtccaagc ctactccacc gctggaggga aggtgtggct
      841 gtcagtgctc ttcatattca gaatcctgct cctggggaca gctgttgagt cagcttgggg
      901 tgatgaacag tctgcctttc gctgtaacac tcaacaacct ggctgcgaaa acgtctgcta
      961 tgacaagtcc ttccccatct ctcacgtgcg cttctgggtc cttcagatca tattcgtgtc
     1021 tgtgcccaca ctcctgtact tggcccatgt gttctatgtg atgaggaagg aagagaagct
     1081 aaacaagaaa gaagaggagc tcaaagtggc ccagactgac ggggtcaacg tggagatgca
     1141 cctgaagcag attgaaatca agaagttcaa gtacgggatt gaagagcacg gcaaggtgaa
     1201 aatgaggggc ggcttgctga gaacctacat catcagcatc ctcttcaagt ctgtcttcga
     1261 ggtggccttc ctgctcatcc agtggtacat ctatgggttc agcttgagcg cggtctacac
     1321 ctgcaagaga gatccctgcc cccaccaggt agactgcttc ctctcacgtc ccacggagaa
     1381 aaccatcttc atcatcttca tgctggtggt gtccttggtg tctctcgctt tgaacatcat
     1441 tgagctcttc tacgtcttct tcaagggcgt taaggatcgc gtgaagggaa gaagcgatcc
     1501 ttaccacgcc accactggcc cactgagccc atcaaaagac tgcggatctc caaaatacgc
     1561 ctacttcaat ggctgctcct caccaacggc tccactctcg cctatgtctc ctcctgggta
     1621 caagctggtt actggtgaca gaaacaattc ctcgtgccgc aattacaaca agcaagctag
     1681 cgagcaaaac tgggcgaact acagcgcaga gcaaaatcgc atggggcagg ccggaagcac
     1741 catctccaac tcgcacgccc agccgttcga tttccccgac gacaaccaga atgccaaaaa
     1801 agttgctgct ggacatgaac tccagccatt agccatcgtg gaccaacgac cttccagcag
     1861 agccagcagc cgcgccagca gcaggcctcg gcctgatgac ctggagattg cggatccacc
     1921 ggtcgccacc atggtgagca agggcgaggg gcaatcgaag catgggctaa aggaggagat
     1981 gacggtgaag taccacatgg aaggttgcgt taatggtcac aaatttgtca ttactggaga
     2041 gggcattgga aaccctttta agggtaagca aactgcaaat ttgtgtgtga tagaaggagg
     2101 cccgctgccg ttctcggagg acattttaag tccgggtttt aaatatggtg accggatttt
     2161 cacagagtac ccacaggata ttgtagatta cttcaagaac tcatgtccag cgggctatac
     2221 gtgggaaagg agcttcctct ttgaggacgg agcggtctgt cgatgcaacg tggacataac
     2281 agtctctgaa aaggagaact gcatctatca caaaagtatc ttcagagggg tgaattttcc
     2341 cgccgacggc cccgtaatga agaaaatgac cactaattgg gaagctagta ccgagaaaat
     2401 tgtgcctgtt ccaaagcaag ggatattaaa aggaaaggtc aaaatgtacc tattgctgaa
     2461 ggatggcggt cgttatcatt gccagtttga tacggtatat aaagctaaat cagtgccctc
     2521 caagatgcca gaatggcatt ttatacagca taagctcctt agggaggacc gctctgatgc
     2581 taaaaaccag aaatggcaat taacagaaca tgcaatcgca ggcatggacg agctgtacaa
     2641 gtaagcggcc gcgactctag atcataatca gccataccac atttgtagag gttttacttg
     2701 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
     2761 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     2821 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     2881 tatcttaagg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt
     2941 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
     3001 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
     3061 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
     3121 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
     3181 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
     3241 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
     3301 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact
     3361 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     3421 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     3481 cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     3541 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     3601 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     3661 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     3721 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     3781 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag
     3841 atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca
     3901 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     3961 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     4021 aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg
     4081 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     4141 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     4201 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     4261 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     4321 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     4381 ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc
     4441 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     4501 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     4561 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     4621 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg
     4681 ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg
     4741 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     4801 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg ctaactgaaa
     4861 cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata
     4921 aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact
     4981 ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc
     5041 caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag
     5101 gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa cttcattttt
     5161 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     5221 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     5281 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     5341 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     5401 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     5461 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     5521 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     5581 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     5641 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     5701 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     5761 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     5821 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     5881 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     5941 cccctgattc tgtggataac cgtattaccg ccatgcatta gttattaata gtaatcaatt
     6001 acggggtcat tagttcatag cccatatatg gagttccg
//
