LOCUS       sequence-22667-e         805 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 805)
  TITLE     Jessell Lab Plasmids
REFERENCE   2  (bases 1 to 805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pBS mouse GDF7 [TJ#37]
FEATURES             Location/Qualifiers
     source          1..805
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     10..26
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10..26
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(463..479)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(464..480)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(508..526)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(547..563)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(547..563)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(560..582)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    571..587
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(595..601,602..619,620..625))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    640..661
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(778..795)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggcggccgct ctagaactag tggatcctgc tcttgagcta gggtcctggg cggccagacc
       61 ctccccagac cgcatgctac ctgcagccgc aggcctccac caccatgtct tcgtactgct
      121 tgtagaccac gttgttggcg gcatcgatgt agagaatgct gatgggactg agccttgcgg
      181 gcacgcagca ggaggctggc gcagcgtcgg gcgccatgga gttgagcagc gtctgaatga
      241 tggcgtggtt ggtaggctcc aggtgcgagc gcagaggaaa gtcgcaaacg ccctcgcagt
      301 ggtatgcctc gtagtctaat ggcgcgatga tccagtcgtc ccagcccagc tccttaaagt
      361 ccacgtgcag tgacttgcga ctgcagcggc tccggcctct gcgcccgtgg cccctgcctg
      421 cgccggatcc cccgggctgc aggaattcga tatcaagctt atcgataccg tcgacctcga
      481 gggggggccc ggtaccagct tttgttccct ttagtgaggg ttaatttcga gcttggcgta
      541 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
      601 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
      661 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
      721 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
      781 gctcactgac tcgctgcgct cggtc
//
