LOCUS       sequence-226769-        5764 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses aa397 to C-terminus of PhiC31 integrase fused to PYL CID.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5764)
  AUTHORS   Weinberg BH, Cho JH, Agarwal Y, Pham NTH, Caraballo LD, Walkosz M, 
            Ortega C, Trexler M, Tague N, Law B, Benman WKJ, Letendre J, Beal J,
            Wong WW
  TITLE     High-performance chemical- and light-inducible recombinases in 
            mammalian cells and mice.
  JOURNAL   Nat Commun. 2019 Oct 24;10(1):4845. doi: 10.1038/s41467-019-12800-7.
  PUBMED    31649244
REFERENCE   2  (bases 1 to 5764)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-10-24"; volume: "10(1):4845. doi"; pages: " 
            10.1038/s41467-019-12800-7"
COMMENT     Sequence Label: pBW1606_pCAG-PV1-PYL-L1-PhiC31-397C-BGHpA
FEATURES             Location/Qualifiers
     source          1..5764
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     365..387
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     449..468
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      570..579
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1116..1142
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1812..1832
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1842..2066
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(2306..2325)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2371..2426
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2425..2444)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(2787..2803)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2787..2803)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2800..2822)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2811..2827
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2835..2841,2842..2859,2860..2865))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2880..2901
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      2986..3120
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3140..3274
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3177..3196)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3231..3250
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3342..3359)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3513..4101)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3593..3612)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4272..5063,5064..5132))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4895..4914
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5133..5237)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        5268..5647
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 caggtggggg tgccgggcgg ggcggggccg cctcgggccg gggagggctc gggggagggg
       61 cgcggcggcc cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt
      121 ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg tgcggagccg
      181 aaatctggga ggcgccgccg caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc
      241 ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc
      301 cctctccagc ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg
      361 gcggggttcg gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat
      421 gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat
      481 tttggcaaag aattgattaa ttcgagcgaa ggcgcgccca ttactcgcat ccattctcag
      541 gctgtctcgt ctcgtctcgc acgcgtgccg ccaccatggc gccaactcaa gacgagttca
      601 cccaactctc ccaatcaatc gccgagttcc acacgtacca actcggtaac ggccgttgct
      661 catctctcct agctcagcga atccacgcgc cgccggaaac agtatggtcc gtggtgagac
      721 gtttcgatag gccacagatt tacaaacact tcatcaaaag ctgtaacgtg agtgaagatt
      781 tcgagatgcg agtgggatgc acgcgcgacg tgaacgtgat aagtggatta ccggcgaata
      841 cgtctcgaga gagattagat ctgttggacg atgatcggag agtgactggg tttagtataa
      901 ccggtggtga acataggctg aggaattata aatcggttac gacggttcat agatttgaga
      961 aagaagaaga agaagaaagg atctggaccg ttgttttgga atcttatgtt gttgatgtac
     1021 cggaaggtaa ttcggaggaa gatacgagat tgtttgctga tacggttatt agattgaatc
     1081 ttcagaaact tgcttcgatc actgaagcta tgaactaccc atacgatgtt ccagattacg
     1141 cttccggagg gtctggctcc ggatcaagtg gtggcagcgg taccgcccct ggccagcacg
     1201 agggcacctg taatgtgagc atggccgccc tggacaagtt cgtggccgag cggatcttca
     1261 acaagatccg gcacgccgag ggcgacgagg agaccctggc cctgctgtgg gaggccgcca
     1321 gaagattcgg caagctgacc gaggcccccg agaagagcgg cgagagggcc aacctggtgg
     1381 ccgagagagc cgacgccctg aacgccctgg aggagctgta cgaggacaga gccgccggag
     1441 cctatgacgg ccctgtgggc aggaagcact tcagaaagca gcaggccgcc ctgaccctga
     1501 gacagcaggg cgccgaggaa agactggccg agctggaggc cgccgaggcc cctaagctgc
     1561 ccctggatca gtggttcccc gaggatgccg acgccgaccc caccggcccc aagtcctggt
     1621 ggggcagagc cagcgtggac gacaagaggg tgttcgtggg cctgttcgtg gataagatcg
     1681 tggtgaccaa gagcaccacc ggcaggggcc agggcacccc catcgagaag agagccagca
     1741 tcacctgggc caagcctccc accgacgacg acgaggatga cgcccaggac ggcaccgagg
     1801 acgtggccgc ccccaagaaa aagcggaagg tgtgagaatt cctgtgcctt ctagttgcca
     1861 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     1921 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     1981 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     2041 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag
     2101 ggggtatccc cacgcgccct gtagcgtgta ccgctgggag ttcgtagacg gaaacaaacg
     2161 cagaatccaa gcgcggccgc ccaggataca tagattacca caactccgag cccttccacc
     2221 gctagcgcac tcctcaggtg caggctgcct atcagaaggt ggtggctggt gtggccaatg
     2281 ccctggctca caaataccac tgagatcttt ttccctctgc caaaaattat ggggacatca
     2341 tgaagcccct tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag
     2401 tgtgttggaa ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttaa
     2461 aacatcagaa tgagtatttg gtttagagtt tggcaacata tgcccatatg ctggctgcca
     2521 tgaacaaagg ttggctataa agaggtcatc agtatatgaa acagccccct gctgtccatt
     2581 ccttattcca tagaaaagcc ttgacttgag gttagatttt ttttatattt tgttttgtgt
     2641 tatttttttc tttaacatcc ctaaaatttt tcttacatgt tttactagcc agatttttcc
     2701 tcctctcctg actactccca gtcatagctg tccctcttct cttatggaga tccctcgacc
     2761 tgcagcccaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     2821 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     2881 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     2941 ctgtcgtgcc agcggatccg catctcaatt agtcagcaac catagtcccg cccctaactc
     3001 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     3061 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     3121 taggcttttg caaaaagcta acttgtttat tgcagcttat aatggttaca aataaagcaa
     3181 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     3241 caaactcatc aatgtatctt atcatgtctg gatccgctgc attaatgaat cggccaacgc
     3301 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     3361 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     3421 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     3481 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     3541 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     3601 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     3661 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     3721 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     3781 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     3841 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     3901 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     3961 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     4021 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     4081 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     4141 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     4201 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     4261 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     4321 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     4381 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     4441 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     4501 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     4561 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     4621 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     4681 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     4741 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     4801 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     4861 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     4921 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     4981 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     5041 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     5101 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     5161 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     5221 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgggtcgac attgattatt
     5281 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
     5341 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
     5401 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
     5461 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
     5521 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
     5581 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     5641 taccatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc
     5701 acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg
     5761 gggg
//
