LOCUS       sequence-22677-e         749 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Pierani A, Brenner-Morton S, Chiang C, Jessell TM
  TITLE     A sonic hedgehog-independent, retinoid-activated pathway of 
            neurogenesis in the ventral spinal cord.
  JOURNAL   Cell. 1999 Jun 25. 97(7):903-15.
  PUBMED    10399918
REFERENCE   2  (bases 1 to 749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 1999 
            Jun 25. 97(7):903-15."
COMMENT     Sequence Label: pBS cE1 (5' dbx2 mouse cDNA) [TJ#53]
FEATURES             Location/Qualifiers
     source          1..749
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(450..466)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(451..467)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(496..516)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(496..514)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(535..551)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(535..551)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(548..570)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    559..575
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(583..589,590..607,608..613))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    628..649
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ggctgcagga attcccacgc acgcaccccc gcccccacgc ccagcgccgg gtcctgagtg
       61 tccgcagctg cggccactgc ctgccagccc agtgccgctc aagctgtgcc cggccggacc
      121 ctttggtgtg cgctgggcct tccagatgcc ccctggccgg gctccggggg agcgggacag
      181 tgccttccag ccctcagccc caggagaaga acacgggctg cctctgctga cccaggactc
      241 aaattccaaa gctcgcaggg ggattttacg aagagctgtg ttctcagagg agcagagaaa
      301 ggctctggag aaaatgtttc agaagcagaa atacatcagc aaaacagacc gaaggaaact
      361 cgcggtcagc ttgggactga aggagtcaca ggtgaagatt tggttccaga accgaaggat
      421 gaaatggcgg aattcgatat caagcttatc gataccgtcg acctcgaggg ggggcccggt
      481 acccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat catggtcata
      541 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccgggag
      601 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
      661 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
      721 acgcgcgggg gagaggcggt ttgcgtatt
//
