LOCUS       sequence-226779-        7399 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of PSD3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7399)
  TITLE     Kahn expression plasmids
REFERENCE   2  (bases 1 to 7399)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: g11s pD42
FEATURES             Location/Qualifiers
     source          1..7399
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     34..53
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     137..156
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        137..155
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    156..180
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    221..245
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1863..1887)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1907..1948
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             1958..1975
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(2032..2050)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      2046..2093
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(2268..2273,2274..2290,2291..2296))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(2327..2345)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2413..2517
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2518..2586,2587..3378)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2736..2755)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3549..4137
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4038..4057
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4291..4308
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4323..4462
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(4388..4407)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4548..4570
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(4564..4755)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(6067..7149)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     7111..7130
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(7150..7227)
                     /gene="lacI"
                     /label=lacI promoter
ORIGIN
        1 gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc
       61 aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat
      121 ctcgatcccg cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc
      181 cctctagaaa taattttgtt taactttaag aaggaatatc acaagtttgt acaaaaaagc
      241 aggcttagaa ggagatagaa ccatgggcta ttctagtggc gtcaccaatg ggctgaatga
      301 tgccagcgac tccatctaca cgaaaggcac cccggagatt gctttctggg gaagcaatgc
      361 tggggtgaaa acaacacggc tagaagctca ttctgaaatg gggagcactg aaattttgga
      421 aaaggagacc ccagaaaatc tcagtaatgg taccagcagc aatgtggaag cagccaaaag
      481 gttggccaaa cgcctttatc agctggacag attcaaaaga tcagatgttg caaaacacct
      541 tggcaagaac aacgaattta gcaaactagt tgcagaagaa tatctgaagt tttttgattt
      601 tacaggaatg acgctggatc agtcactcag gtatttcttt aaagcattct ctcttgtggg
      661 agaaactcaa gaacgagaga gagttttaat acacttctcc aatagatatt tttattgtaa
      721 cccagatacc attgcttcac aagatggagt ccattgcctt acctgtgcaa taatgcttct
      781 taataccgat ctacatggcc ataatattgg aaagaagatg acctgtcagg agttcattgc
      841 aaatctgcaa ggggtaaatg agggtgttga tttctccaag gatctgctga aagctctgta
      901 caactcaatc aagaatgaga agcttgaatg ggcagtagat gatgaagaga aaaaaaagtc
      961 tcccccagaa agtactgagg agaaagctaa cggaacacat ccaaagacca tcagtcgtat
     1021 tggaagtact actaacccat ttttggacat tcctcatgat ccaaatgctg ctgtgtacaa
     1081 aagtggattc ttggctcgga aaattcatgc agatatggat ggaaagaaga ctccaagagg
     1141 aaaacgagga tggaaaacct tttatgctgt actgaaggga acagttcttt acttgcaaaa
     1201 ggatgaatac aagccagaaa aggccttgtc tgaagaggac ttgaaaaacg ctgtgagtgt
     1261 gcaccacgca ttggcatcca aggccacgga ctatgagaag aaaccaaacg tgtttaaact
     1321 taaaactgcc gactggaggg tcttgctttt tcaaactcag agcccagagg aaatgcaagg
     1381 gtggataaac aaaatcaatt gtgtggcagc tgtattttct gcaccaccat ttccagcagc
     1441 aatcggctct cagaagaagt ttagccgccc acttctgcct gccactacaa caaaactgtc
     1501 tcaggaggag caactgaagt cacatgaaag taagctgaag cagatcacca ccgagctggc
     1561 cgagcaccgc tcatatcccc ccgacaagaa ggtcaaagcc aaggacgtcg atgagtacaa
     1621 actgaaagac cactatctgg agtttgagaa aacccgctat gaaatgtatg tcagcattct
     1681 caaggaagga ggcaaagagc tactgagtaa cgatgaaagc gaggctgcag gactgaagaa
     1741 gtcgcactcg agtccttcgc tgaacccgga tacttctcca atcactgcca aagtcaagcg
     1801 taacgtgtca gagaggaagg atcaccgacc tgaaacacca agcattaagc aaaaagttac
     1861 tgacccagct ttcttgtaca aagtggtgat caattcgaag cttgaaggta agcctatccc
     1921 taaccctctc ctcggtctcg attctacgcg taccggtcat catcaccatc accattgagt
     1981 ttgatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc
     2041 aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag
     2101 gaggaactat atccggatat cccgcaagag gcccggcagt accggcataa ccaagcctat
     2161 gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat
     2221 acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc
     2281 gatgataagc tgtcaaacat gagaattaat tcttgaagac gaaagggcct cgtgatacgc
     2341 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     2401 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     2461 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     2521 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     2581 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     2641 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     2701 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     2761 gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     2821 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     2881 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     2941 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     3001 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     3061 gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     3121 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     3181 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     3241 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     3301 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     3361 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     3421 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     3481 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     3541 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     3601 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     3661 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     3721 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     3781 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     3841 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     3901 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     3961 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     4021 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     4081 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     4141 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     4201 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     4261 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     4321 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg caatggtgca
     4381 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct
     4441 acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg
     4501 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     4561 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc
     4621 agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag
     4681 tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt
     4741 ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat
     4801 accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt
     4861 actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat
     4921 cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca
     4981 gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc
     5041 cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt
     5101 tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt
     5161 aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg
     5221 tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc
     5281 gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt
     5341 ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc
     5401 gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg
     5461 gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg
     5521 acgatcagcg gtccaatgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc
     5581 tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg
     5641 atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac
     5701 gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg
     5761 ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg
     5821 aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa
     5881 atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata
     5941 agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct
     6001 ctcaagggca tcggtcgaga tcccggtgcc taatgagtga gctaacttac attaattgcg
     6061 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     6121 ggccaacgcg cggggagagg cggtttgcgt attgggcgcc agggtggttt ttcttttcac
     6181 cagtgagacg ggcaacagct gattgccctt caccgcctgg ccctgagaga gttgcagcaa
     6241 gcggtccacg ctggtttgcc ccagcaggcg aaaatcctgt ttgatggtgg ttaacggcgg
     6301 gatataacat gagctgtctt cggtatcgtc gtatcccact accgagatat ccgcaccaac
     6361 gcgcagcccg gactcggtaa tggcgcgcat tgcgcccagc gccatctgat cgttggcaac
     6421 cagcatcgca gtgggaacga tgccctcatt cagcatttgc atggtttgtt gaaaaccgga
     6481 catggcactc cagtcgcctt cccgttccgc tatcggctga atttgattgc gagtgagata
     6541 tttatgccag ccagccagac gcagacgcgc cgagacagaa cttaatgggc ccgctaacag
     6601 cgcgatttgc tggtgaccca atgcgaccag atgctccacg cccagtcgcg taccgtcttc
     6661 atgggagaaa ataatactgt tgatgggtgt ctggtcagag acatcaagaa ataacgccgg
     6721 aacattagtg caggcagctt ccacagcaat ggcatcctgg tcatccagcg gatagttaat
     6781 gatcagccca ctgacgcgtt gcgcgagaag attgtgcacc gccgctttac aggcttcgac
     6841 gccgcttcgt tctaccatcg acaccaccac gctggcaccc agttgatcgg cgcgagattt
     6901 aatcgccgcg acaatttgcg acggcgcgtg cagggccaga ctggaggtgg caacgccaat
     6961 cagcaacgac tgtttgcccg ccagttgttg tgccacgcgg ttgggaatgt aattcagctc
     7021 cgccatcgcc gcttccactt tttcccgcgt tttcgcagaa acgtggctgg cctggttcac
     7081 cacgcgggaa acggtctgat aagagacacc ggcatactct gcgacatcgt ataacgttac
     7141 tggtttcaca ttcaccaccc tgaattgact ctcttccggg cgctatcatg ccataccgcg
     7201 aaaggttttg cgccattcga tggtgtccgg gatctcgacg ctctccctta tgcgactcct
     7261 gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg
     7321 gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca
     7381 cgccgaaaca agcgctcat
//
