LOCUS       sequence-226784-       15930 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus transfer vector for expression of huntingtin protein in 
            insect and mammalian cellscells as well as in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15930)
  AUTHORS   Harding RJ, Loppnau P, Ackloo S, Lemak A, Hutchinson A, Hunt B, 
            Holehouse AS, Ho JC, Fan L, Toledo-Sherman L, Seitova A, Arrowsmith 
            CH
  TITLE     Design and characterization of mutant and wildtype huntingtin 
            proteins produced from a toolkit of scalable eukaryotic expression 
            systems.
  JOURNAL   J Biol Chem. 2019 Apr 26;294(17):6986-7001. doi: 
            10.1074/jbc.RA118.007204. Epub 2019 Mar 6.
  PUBMED    30842263
REFERENCE   2  (bases 1 to 15930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.007204"; journalName: "J Biol Chem"; date: 
            "2019-04-26- 26"; volume: "294"; issue: "17"; pages: "6986-7001"
COMMENT     Sequence Label: pBacMam2-DiEx-LIC-C-flag_huntingtin_full-lengt...
FEATURES             Location/Qualifiers
     source          1..15930
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      102..556
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(189..208)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     398..419
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        582..686
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(687..755,756..1547)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(905..924)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1718..2306
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2207..2226
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2460..2477
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  2611..2835
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2902..3435)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3624..3629,3630..3646,3647..3652))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     polyA_signal    4160..4208
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     4233..4252
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     enhancer        4313..4692
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4693..4896
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4847..4867
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        4943..5052
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     regulatory      13983..13992
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             14678..14701
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             14723..14752
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             14753..14776
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(14866..14886)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(15284..15295)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    15520..15654
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(15557..15576)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     15611..15630
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  15683..15848
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga
       61 ctgaatatca acgcttattt gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
      121 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
      181 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
      241 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
      301 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
      361 ttttcgccct ttgacgttgg agtccacgtt cttaatagtg gactcttgtt ccaaactgga
      421 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
      481 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
      541 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
      601 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
      661 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
      721 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
      781 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
      841 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
      901 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
      961 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     1021 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     1081 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     1141 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     1201 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     1261 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     1321 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     1381 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     1441 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     1501 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     1561 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     1621 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     1681 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     1741 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     1801 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     1861 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     1921 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     1981 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     2041 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     2101 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     2161 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     2221 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     2281 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     2341 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     2401 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     2461 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc
     2521 tgtgcggtat ttcacaccgc atagaccagc cgcgtaacct ggcaaaatcg gttacggttg
     2581 agtaataaat ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa
     2641 caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga
     2701 aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact
     2761 cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg
     2821 gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc
     2881 gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac
     2941 ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc
     3001 ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttcaaga gattgatgag
     3061 cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat
     3121 ctcactacgc ggctgctcaa acttgggcag aacgtaagcc gcgagagcgc caacaaccgc
     3181 ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta
     3241 atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag
     3301 atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat
     3361 gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt
     3421 gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg
     3481 cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga
     3541 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg
     3601 agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc
     3661 gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg
     3721 aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg
     3781 cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg cccttgcttc
     3841 aggagatcgg tagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag
     3901 tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag gactctagct
     3961 atagttctag tggttggcta cgtacccgta gtggctatgg cagggcttgc cgccccgacg
     4021 ttggctgcga gccctgggcc ttcacccgaa cttgggggtt ggggtgggga aaaggaagaa
     4081 acgcgggcgt attggtccca atggggtctc ggtggggtat cgacagagtg ccagccctgg
     4141 gaccgaaccc cgcgtttatg aacaaacgac ccaacacccg tgcgttttat tctgtctttt
     4201 tattgccgtc atagcgcggg ttccttccgg tattgtctcc ttccgtgttt cagttagcct
     4261 cccccatctc ccggtaccgc atgcggatct gtacgggcca gatatacgcg ttgacattga
     4321 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     4381 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     4441 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     4501 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     4561 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     4621 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     4681 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     4741 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     4801 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     4861 aggcgtgtac ggtgggaggt ctatataagc agagctctct ggctaactag agaacccact
     4921 gcttactggc ttatcgaata cggaccttta attcaaccca acacaatata ttatagttaa
     4981 ataagaatta ttatcaaatc atttgtatat taattaaaat actatactgt aaattacatt
     5041 ttatttacaa tcactcgacg gatccttaag aaggagatat actatggcga ccctggaaaa
     5101 gctgatgaag gccttcgagt ccctcaagtc cttccaacag cagcaacagc aacaacagca
     5161 gcaacagcaa caacagcagc aacagcaaca acagcagcaa cagcaacaac agcagcaaca
     5221 gcaacaacag cagcaacagc aacaacagca gcaacagcaa caacagcagc aacagcaaca
     5281 acagcagcaa cagcaacaac agcagcaaca gcaacaacag cagcaacagc aacaacagca
     5341 gcaacagcaa caaccgccac cgccgccgcc gccgccgccg cctcctcagc ttcctcagcc
     5401 gccgccgcag gcacagccgc tgctgcctca gccgcagccg cccccgccgc cgcccccgcc
     5461 gccacccggc ccggctgtgg ctgaggagcc gctgcaccga ccaaagaaag aactttcagc
     5521 taccaagaaa gaccgtgtga atcattgtct gacaatatgt gaaaacatag tggcacagtc
     5581 tgtcagaaat tctccagaat ttcagaaact tctgggcatc gctatggaac tttttctgct
     5641 gtgcagtgat gacgcagagt cagatgtcag gatggtggct gacgaatgcc tcaacaaagt
     5701 tatcaaagct ttgatggatt ctaatcttcc aaggttacag ctcgagctct ataaggaaat
     5761 taaaaagaat ggtgcccctc ggagtttgcg tgctgccctg tggaggtttg ctgagctggc
     5821 tcacctggtt cggcctcaga aatgcaggcc ttacctggtg aaccttctgc cgtgcctgac
     5881 tcgaacaagc aagagacccg aagaatcagt ccaggagacc ttggctgcag ctgttcccaa
     5941 aattatggct tcttttggca attttgcaaa tgacaatgaa attaaggttt tgttaaaggc
     6001 cttcatagcg aacctgaagt caagctcccc caccattcgg cggacagcgg ctggatcagc
     6061 agtgagcatc tgccagcact caagaaggac acaatatttc tatagttggc tactaaatgt
     6121 gctcttaggc ttactcgttc ctgtcgagga tgaacactcc actctgctga ttcttggcgt
     6181 gctgctcacc ctgaggtatt tggtgccctt gctgcagcag caggtcaagg acacaagcct
     6241 gaaaggcagc ttcggagtga caaggaaaga aatggaagtc tctccttctg cagagcagct
     6301 tgtccaggtt tatgaactga cgttacatca tacacagcac caagaccaca atgttgtgac
     6361 cggagccctg gagctgttgc agcagctctt cagaacgcct ccacccgagc ttctgcaaac
     6421 cctgaccgca gtcgggggca ttgggcagct caccgctgct aaggaggagt ctggtggccg
     6481 aagccgtagt gggagtattg tggaacttat agctggaggg ggttcctcat gcagccctgt
     6541 cctttcaaga aaacaaaaag gcaaagtgct cttaggagaa gaagaagcct tggaggatga
     6601 ctctgaatcg agatcggatg tcagcagctc tgccttaaca gcctcagtga aggatgagat
     6661 cagtggagag ctggctgctt cttcaggggt ttccactcca gggtcagcag gtcatgacat
     6721 catcacagaa cagccacggt cacagcacac actgcaggcg gactcagtgg atctggccag
     6781 ctgtgacttg acaagctctg ccactgatgg ggatgaggag gatatcttga gccacagctc
     6841 cagccaggtc agcgccgtcc catctgaccc tgccatggac ctgaatgatg ggacccaggc
     6901 ctcgtcgccc atcagcgaca gctcccagac caccaccgaa gggcctgatt cagctgttac
     6961 cccttcagac agttctgaaa ttgtgttaga cggtaccgac aaccagtatt tgggcctgca
     7021 gattggacag ccccaggatg aagatgagga agccacaggt attcttcctg atgaagcctc
     7081 ggaggccttc aggaactctt ccatggccct tcaacaggca catttattga aaaacatgag
     7141 tcactgcagg cagccttctg acagcagtgt tgataaattt gtgttgagag atgaagctac
     7201 tgaaccgggt gatcaagaaa acaagccttg ccgcatcaaa ggtgacattg gacagtccac
     7261 tgatgatgac tctgcacctc ttgtccattg tgtccgcctt ttatctgctt cgtttttgct
     7321 aacaggggga aaaaatgtgc tggttccgga cagggatgtg agggtcagcg tgaaggccct
     7381 ggccctcagc tgtgtgggag cagctgtggc cctccacccg gaatctttct tcagcaaact
     7441 ctataaagtt cctcttgaca ccacggaata ccctgaggaa cagtatgtct cagacatctt
     7501 gaactacatc gatcatggag acccacaggt tcgaggagcc actgccattc tctgtgggac
     7561 cctcatctgc tccatcctca gcaggtcccg cttccacgtg ggagattgga tgggcaccat
     7621 tagaaccctc acaggaaata cattttcttt ggcggattgc attcctttgc tgcggaaaac
     7681 actgaaggat gagtcttctg ttacttgcaa gttagcttgt acagctgtga ggaactgtgt
     7741 catgagtctc tgcagcagca gctacagtga gttaggactg cagctgatca tcgatgtgct
     7801 gactctgagg aacagttcct attggctggt gaggacagag cttctggaaa cccttgcaga
     7861 gattgacttc aggctggtga gctttttgga ggcaaaagca gaaaacttac acagaggggc
     7921 tcatcattat acagggcttt taaaactgca agaacgagtg ctcaataatg ttgtcatcca
     7981 tttgcttgga gatgaagacc ccagggtgcg acatgttgcc gcagcatcac taattaggct
     8041 tgtcccaaag ctgttttata aatgtgacca aggacaagct gatccagtag tggccgtggc
     8101 aagagatcaa agcagtgttt acctgaaact tctcatgcat gagacgcagc ctccatctca
     8161 tttctccgtc agcacaataa ccagaatata tagaggctat aacctactac caagcataac
     8221 agacgtcact atggaaaata acctttcaag agttattgca gcagtttctc atgaactaat
     8281 cacatcaacc accagagcac tcacatttgg atgctgtgaa gctttgtgtc ttctttccac
     8341 tgccttccca gtttgcattt ggagtttagg ttggcactgt ggagtgcctc cactgagtgc
     8401 ctcagatgag tctaggaaga gctgtaccgt tgggatggcc acaatgattc tgaccctgct
     8461 ctcgtcagct tggttcccat tggatctctc agcccatcaa gatgctttga ttttggccgg
     8521 aaacttgctt gcagccagtg ctcccaaatc tctgagaagt tcatgggcct ctgaagaaga
     8581 agccaaccca gcagccacca agcaagagga ggtctggcca gccctggggg accgggccct
     8641 ggtgcccatg gtggagcagc tcttctctca cctgctgaag gtgattaaca tttgtgccca
     8701 cgtcctggat gacgtggctc ctggacccgc aataaaggca gccttgcctt ctctaacaaa
     8761 ccccccttct ctaagtccca tccgacgaaa ggggaaggag aaagaaccag gagaacaagc
     8821 atctgtaccg ttgagtccca agaaaggcag tgaggccagt gcagcttcta gacaatctga
     8881 tacctcaggt cctgttacaa caagtaaatc ctcatcactg gggagtttct atcatcttcc
     8941 ttcatacctc aaactgcatg atgtcctgaa agctacacac gctaactaca aggtcacgct
     9001 ggatcttcag aacagcacgg aaaagtttgg agggtttctt cgctcagcct tggatgttct
     9061 ttctcagata ctagagctgg ccacactgca ggacattggg aagtgtgttg aagagatcct
     9121 aggatacctg aaatcctgct ttagtcgaga accaatgatg gcaactgttt gtgttcaaca
     9181 attgttgaag actctctttg gcacaaactt ggcctcccag tttgatggct tatcttccaa
     9241 ccccagcaag tcacaaggcc gagcacagcg ccttggctcc tccagtgtga ggccaggctt
     9301 gtaccactac tgcttcatgg ccccgtacac ccacttcacc caggccctcg ctgacgccag
     9361 cctgaggaac atggtgcagg cggagcagga gaacgacacc tcgggatggt ttgatgtcct
     9421 ccagaaagtg tctacccagt tgaagacaaa cctcacgagt gtcacaaaga accgtgcaga
     9481 taagaatgct attcataatc acattcgttt gtttgaacct cttgttataa aagctttaaa
     9541 acagtacacg actacaacat gtgtgcagtt acagaagcag gttttagatt tgctggcgca
     9601 gctggttcag ttacgggtta attactgtct tctggattca gatcaggtgt ttattggctt
     9661 tgtattgaaa cagtttgaat acattgaagt gggccagttc agggaatcag aggcaatcat
     9721 tccaaacatc tttttcttct tggtattact atcttatgaa cgctatcatt caaaacagat
     9781 cattggaatt cctaaaatca ttcagctctg tgatggcatc atggccagtg gaaggaaggc
     9841 tgtgacacat gccataccgg ctctgcagcc catagtccac gacctctttg tattaagagg
     9901 aacaaataaa gctgatgcag gaaaagagct tgaaacccaa aaagaggtgg tggtgtcaat
     9961 gttactgaga ctcatccagt accatcaggt gttggagatg ttcattcttg tcctgcagca
    10021 gtgccacaag gagaatgaag acaagtggaa gcgactgtct cgacagatag ctgacatcat
    10081 cctcccaatg ttagccaaac agcagatgca cattgactct catgaagccc ttggagtgtt
    10141 aaatacatta tttgagattt tggccccttc ctccctccgt ccggtagaca tgcttttacg
    10201 gagtatgttc gtcactccaa acacaatggc gtccgtgagc actgttcaac tgtggatatc
    10261 gggaattctg gccattttga gggttctgat ttcccagtca actgaagata ttgttctttc
    10321 tcgtattcag gagctctcct tctctccgta tttaatctcc tgtacagtaa ttaataggtt
    10381 aagagatggg gacagtactt caacgctaga agaacacagt gaagggaaac aaataaagaa
    10441 tttgccagaa gaaacatttt caaggtttct attacaactg gttggtattc ttttagaaga
    10501 cattgttaca aaacagctga aggtggaaat gagtgagcag caacatactt tctattgcca
    10561 ggaactaggc acactgctaa tgtgtctgat ccacatcttc aagtctggaa tgttccggag
    10621 aatcacagca gctgccacta ggctgttccg cagtgatggc tgtggcggca gtttctacac
    10681 cctggacagc ttgaacttgc gggctcgttc catgatcacc acccacccgg ccctggtgct
    10741 gctctggtgt cagatactgc tgcttgtcaa ccacaccgac taccgctggt gggcagaagt
    10801 gcagcagacc ccgaaaagac acagtctgtc cagcacaaag ttacttagtc cccagatgtc
    10861 tggagaagag gaggattctg acttggcagc caaacttgga atgtgcaata gagaaatagt
    10921 acgaagaggg gctctcattc tcttctgtga ttatgtctgt cagaacctcc atgactccga
    10981 gcacttaacg tggctcattg taaatcacat tcaagatctg atcagccttt cccacgagcc
    11041 tccagtacag gacttcatca gtgccgttca tcggaactct gctgccagcg gcctgttcat
    11101 ccaggcaatt cagtctcgtt gtgaaaacct ttcaactcca accatgctga agaaaactct
    11161 tcagtgcttg gaggggatcc atctcagcca gtcgggagct gtgctcacgc tgtatgtgga
    11221 caggcttctg tgcacccctt tccgtgtgct ggctcgcatg gtcgacatcc ttgcttgtcg
    11281 ccgggtagaa atgcttctgg ctgcaaattt acagagcagc atggcccagt tgccaatgga
    11341 agaactcaac agaatccagg aataccttca gagcagcggg ctcgctcaga gacaccaaag
    11401 gctctattcc ctgctggaca ggtttcgtct ctccaccatg caagactcac ttagtccctc
    11461 tcctccagtc tcttcccacc cgctggacgg ggatgggcac gtgtcactgg aaacagtgag
    11521 tccggacaaa gactggtacg ttcatcttgt caaatcccag tgttggacca ggtcagattc
    11581 tgcactgctg gaaggtgcag agctggtgaa tcggattcct gctgaagata tgaatgcctt
    11641 catgatgaac tcggagttca acctaagcct gctagctcca tgcttaagcc tagggatgag
    11701 tgaaatttct ggtggccaga agagtgccct ttttgaagca gcccgtgagg tgactctggc
    11761 ccgtgtgagc ggcaccgtgc agcagctccc tgctgtccat catgtcttcc agcccgagct
    11821 gcctgcagaa ccggcggcct actggagcaa gttgaatgat ctgtttgggg atgctgcact
    11881 gtatcagtcc ctgcccactc tggcccgggc cctggcacag tacctggtgg tggtctccaa
    11941 actgcccagt catttgcacc ttcctcctga gaaagagaag gacattgtga aattcgtggt
    12001 ggcaaccctt gaggccctgt cctggcattt gatccatgag cagatcccgc tgagtctgga
    12061 tctccaggca gggctggact gctgctgcct ggccctgcag ctgcctggcc tctggagcgt
    12121 ggtctcctcc acagagtttg tgacccacgc ctgctccctc atccactgtg tgcacttcat
    12181 cctggaggcc gttgcagtgc agcctggaga gcagcttctt agtccagaaa gaaggacaaa
    12241 taccccaaaa gccatcagcg aggaggagga ggaagtagat ccaaacacac agaatcctaa
    12301 gtatatcact gcagcctgtg agatggtggc agaaatggtg gagtctctgc agtcggtgtt
    12361 ggccttgggt cataaaagga atagcggcgt gccggcgttt ctcacgccat tgctcaggaa
    12421 catcatcatc agcctggccc gcctgcccct tgtcaacagc tacacacgtg tgcccccact
    12481 ggtgtggaag cttggatggt cacccaaacc gggaggggat tttggcacag cattccctga
    12541 gatccccgtg gagttcctcc aggaaaagga agtctttaag gagttcatct accgcatcaa
    12601 cacactaggc tggaccagtc gtactcagtt tgaagaaact tgggccaccc tccttggtgt
    12661 cctggtgacg cagcccctcg tgatggagca ggaggagagc ccaccagaag aagacacaga
    12721 gaggacccag atcaacgtcc tggccgtgca ggccatcacc tcactggtgc tcagtgcaat
    12781 gactgtgcct gtggccggca acccagctgt aagctgcttg gagcagcagc cccggaacaa
    12841 gcctctgaaa gctctcgaca ccaggtttgg gaggaagctg agcattatca gagggattgt
    12901 ggagcaagag attcaagcaa tggtttcaaa gagagagaat attgccaccc atcatttata
    12961 tcaggcatgg gatcctgtcc cttctctgtc tccggctact acaggtgccc tcatcagcca
    13021 cgagaagctg ctgctgcaga tcaaccccga gcgggagctg gggagcatga gctacaaact
    13081 cggccaggtg tccatacact ccgtgtggct agggaacagc atcacacccc tgagggagga
    13141 ggaatgggac gaggaagagg aggaggaggc cgacgcccct gcaccttcgt caccacccac
    13201 gtctccagtc aactccagga aacaccgggc tggagttgac atccactcct gttcgcagtt
    13261 tttgcttgag ttgtacagcc gctggatcct gccgtccagc tcagccagga ggaccccggc
    13321 catcctgatc agtgaggtgg tcagatccct tctagtggtc tcagacttgt tcaccgagcg
    13381 caaccagttt gagctgatgt atgtgacgct gacagaactg cgaagggtgc acccttcaga
    13441 agacgagatc ctcgctcagt acctggtgcc tgccacctgc aaggcagctg ccgtccttgg
    13501 gatggacaag gccgtggcgg agcctgtcag ccgcctgctg gagagcacgc tcaggagcag
    13561 ccacctgccc agcagggttg gagccctgca cggcatcctc tatgtgctgg agtgcgacct
    13621 gctggacgac actgccaagc agctcatccc ggtcatcagc gactatctcc tctccaacct
    13681 gaaagggatc gcccactgcg tgaacattca cagccagcag cacgtactgg tcatgtgtgc
    13741 cactgcgttt tacctcattg agaactatcc tctggacgta gggccggaat tttcagcatc
    13801 aataatacag atgtgtgggg tgatgctgtc tggaagtgag gagtccaccc cctccatcat
    13861 ttaccactgt gccctcagag gcctggagcg cctcctgctc tctgagcagc tctcccgcct
    13921 ggatgcagaa tcgctggtca agctgagtgt ggacagagtg aacgtgcaca gcccgcaccg
    13981 ggccatggcg gctctgggcc tgatgctcac ctgcatgtac acaggaaagg agaaagtcag
    14041 tccgggtaga acttcagacc ctaatcctgc agcccccgac agcgagtcag tgattgttgc
    14101 tatggagcgg gtatctgttc tttttgatag gatcaggaaa ggctttcctt gtgaagccag
    14161 agtggtggcc aggatcctgc cccagtttct agacgacttc ttcccacccc aggacatcat
    14221 gaacaaagtc atcggagagt ttctgtccaa ccagcagcca tacccccagt tcatggccac
    14281 cgtggtgtat aaggtgtttc agactctgca cagcaccggg cagtcgtcca tggtccggga
    14341 ctgggtcatg ctgtccctct ccaacttcac gcagagggcc ccggtcgcca tggccacgtg
    14401 gagcctctcc tgcttctttg tcagcgcgtc caccagcccg tgggtcgcgg cgatcctccc
    14461 acatgtcatc agcaggatgg gcaagctgga gcaggtggac gtgaaccttt tctgcctggt
    14521 cgccacagac ttctacagac accagataga ggaggagctc gaccgcaggg ccttccagtc
    14581 tgtgcttgag gtggttgcag ccccaggaag cccatatcac cggctgctga cttgtttacg
    14641 aaatgtccac aaggtcacca cctgcggtgg aagcggtgac tacaaagacg atgacgacaa
    14701 gtaaaacctc tacttccaat cgcaccatca tcaccatcac catcaccacc atgattacaa
    14761 ggatgacgac gataagtgag gcggccgcca acttgatatc aagcttatcg ataatcaacc
    14821 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
    14881 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
    14941 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
    15001 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
    15061 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
    15121 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
    15181 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
    15241 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
    15301 ggaccttcct tcccgcgggc gcccccctcc tcacggcgag cgaagcttgt cgagaagtac
    15361 tagaggatct ctagagcctg cagtctcgac aagcttgtcg agaagtacta gaggatcata
    15421 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
    15481 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
    15541 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
    15601 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatctgatca
    15661 ctgcttgagc ctaggagatc cgaaccagat aagtgaaatc tagttccaaa ctattttgtc
    15721 atttttaatt ttcgtattag cttacgacgc tacacccagt tcccatctat tttgtcactc
    15781 ttccctaaat aatccttaaa aactccattt ccacccctcc cagttcccaa ctattttgtc
    15841 cgcccacagc ggggcatttt tcttcctgtt atgtttttaa tcaaacatcc tgccaactcc
    15901 atgtgacaaa ccgtcatctt cggctacttt
//
