LOCUS       sequence-226786-        8816 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tet-inducible shRNA targeting OGDH.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8816)
  AUTHORS   Ilic N, Birsoy K, Aguirre AJ, Kory N, Pacold ME, Singh S, Moody SE, 
            DeAngelo JD, Spardy NA, Freinkman E, Weir BA, Tsherniak A, Cowley 
            GS, Root DE, Asara JM, Vazquez F, Widlund HR, Sabatini DM, Hahn WC
  TITLE     PIK3CA mutant tumors depend on oxoglutarate dehydrogenase.
  JOURNAL   Proc Natl Acad Sci U S A. 2017 Apr 25;114(17):E3434-E3443. doi: 
            10.1073/pnas.1617922114. Epub 2017 Apr 10.
  PUBMED    28396387
REFERENCE   2  (bases 1 to 8816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1617922114"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2017-04-25- 25"; volume: "114"; issue: "17"; pages: 
            "E3434-E3443"
COMMENT     Sequence Label: pLKO-puro-tetON-shOGDH 2956
FEATURES             Location/Qualifiers
     source          1..8816
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    45..66
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(81..86,87..104,105..111)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    119..135
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     124..146
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     143..159
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     143..159
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     178..198
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        180..198
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        226..452
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             453..633
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    680..805
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1298..1531
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1716..1760
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2049..2143
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     primer_bind     2060..2079
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    2097..2115
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2125..2143
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    2244..2361
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2410..2920
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2839..2857
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     intron          2929..3501
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     3509..3528
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        3556..3574
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3585..4205
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(3658..3677)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     misc_feature    4240..4813
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4407..4424)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4634..4653
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4833..5432
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4833..4852)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5329..5349
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5560..5793
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5865..5986
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5902..5921)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5956..5975
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6026..6161
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(6182..6200)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6210..6227)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6210..6226)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6219..6241)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6368..6823
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6455..6474)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6665..6686
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6849..6953
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6954..7022,7023..7814)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7172..7191)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7985..8573
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8474..8493
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8727..8744
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
       61 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
      121 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca
      181 attaaccctc actaaaggga acaaaagctg gagctgcaag cttaatgtag tcttatgcaa
      241 tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga
      301 aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa
      361 ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata
      421 ttgtatttaa gtgcctagct cgatacataa acgggtctct ctggttagac cagatctgag
      481 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
      541 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
      601 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
      661 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
      721 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
      781 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
      841 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
      901 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
      961 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     1021 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     1081 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     1141 agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat
     1201 tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga
     1261 gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct
     1321 tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac
     1381 aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac
     1441 agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg
     1501 tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca
     1561 tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt
     1621 ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac
     1681 actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat
     1741 tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa
     1801 aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt
     1861 ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa
     1921 ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag
     1981 acagatccat tcgattagtg aacggatctc gacggtatcg atcacgagac tagcctcgag
     2041 cggccgcaat atttgcatgt cgctatgtgt tctgggaaat caccataaac gtgaaatccc
     2101 tatcagtgat agagacttat aagttcccta tcagtgatag agacaccggg actacgtgaa
     2161 gccaagactt ctcgagaagt cttggcttca cgtagtcttt ttgaattctc gacctcgaga
     2221 caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca
     2281 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa
     2341 attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc actttggccg
     2401 cggctcgagg gggttggggt tgcgcctttt ccaaggcagc cctgggtttg cgcagggacg
     2461 cggctgctct gggcgtggtt ccgggaaacg cagcggcgcc gaccctgggt ctcgcacatt
     2521 cttcacgtcc gttcgcagcg tcacccggat cttcgccgct acccttgtgg gccccccggc
     2581 gacgcttcct gctccgcccc taagtcggga aggttccttg cggttcgcgg cgtgccggac
     2641 gtgacaaacg gaagccgcac gtctcactag taccctcgca gacggacagc gccagggagc
     2701 aatggcagcg cgccgaccgc gatgggctgt ggccaatagc ggctgctcag cagggcgcgc
     2761 cgagagcagc ggccgggaag gggcggtgcg ggaggcgggg tgtggggcgg tagtgtgggc
     2821 cctgttcctg cccgcgcggt gttccgcatt ctgcaagcct ccggagcgca cgtcggcagt
     2881 cggctccctc gttgaccgaa tcaccgacct ctctccccag ggggatctgt gagtttgggg
     2941 acccttgatt gttctttctt tttcgctatt gtaaaattca tgttatatgg agggggcaaa
     3001 gttttcaggg tgttgtttag aatgggaaga tgtcccttgt atcaccatgg accctcatga
     3061 taattttgtt tctttcactt tctactctgt tgacaaccat tgtctcctct tattttcttt
     3121 tcattttctg taactttttc gttaaacttt agcttgcatt tgtaacgaat ttttaaattc
     3181 acttttgttt atttgtcaga ttgtaagtac tttctctaat cacttttttt tcaaggcaat
     3241 cagggtatat tatattgtac ttcagcacag ttttagagaa caattgttat aattaaatga
     3301 taaggtagaa tatttctgca tataaattct ggctggcgtg gaaatattct tattggtaga
     3361 aacaactaca tcctggtcat catcctgcct ttctctttat ggttacaatg atatacactg
     3421 tttgagatga ggataaaata ctctgagtcc aaaccgggcc cctctgctaa ccatgttcat
     3481 gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat
     3541 tttggcaaag aattgtaata cgactcacta tagggcgaat tgatatgtct agattagata
     3601 aaagtaaagt gattaacagc gcattagagc tgcttaatga ggtcggaatc gaaggtttaa
     3661 caacccgtaa actcgcccag aagctaggtg tagagcagcc tacattgtat tggcatgtaa
     3721 aaaataagcg ggctttgctc gacgccttag ccattgagat gttagatagg caccatactc
     3781 acttttgccc tttagaaggg gaaagctggc aagatttttt acgtaataac gctaaaagtt
     3841 ttagatgtgc tttactaagt catcgcgatg gagcaaaagt acatttaggt acacggccta
     3901 cagaaaaaca gtatgaaact ctcgaaaatc aattagcctt tttatgccaa caaggttttt
     3961 cactagagaa tgcattatat gcactcagcg ctgtggggca ttttacttta ggttgcgtat
     4021 tggaagatca agagcatcaa gtcgctaaag aagaaaggga aacacctact actgatagta
     4081 tgccgccatt attacgacaa gctatcgaat tatttgatca ccaaggtgca gagccagcct
     4141 tcttattcgg ccttgaattg atcatatgcg gattagaaaa acaacttaaa tgtgaaagtg
     4201 ggtccgcgta cagcggctcc cgggagttct agggatctgc ccctctccct cccccccccc
     4261 taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt
     4321 ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt
     4381 gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt
     4441 cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct
     4501 ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt
     4561 ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt
     4621 ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa
     4681 ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta
     4741 gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa
     4801 aacacgatga taaggatcca ccggagctta ccatgaccga gtacaagccc acggtgcgcc
     4861 tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact
     4921 accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc
     4981 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg
     5041 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg
     5101 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg
     5161 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg
     5221 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg
     5281 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct
     5341 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca
     5401 cctggtgcat gacccgcaag cccggtgcct gacgcccgcc ccacgacccg cagcgcccga
     5461 ccgaaaggag cgcacgaccc catgcatcgg tacctttaag accaatgact tacaaggcag
     5521 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     5581 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     5641 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     5701 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     5761 gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc
     5821 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag
     5881 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     5941 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct
     6001 agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     6061 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     6121 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg
     6181 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
     6241 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     6301 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     6361 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     6421 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     6481 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     6541 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     6601 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     6661 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     6721 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     6781 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg
     6841 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     6901 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     6961 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     7021 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     7081 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     7141 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     7201 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     7261 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     7321 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     7381 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     7441 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     7501 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     7561 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     7621 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     7681 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     7741 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     7801 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     7861 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     7921 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     7981 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     8041 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     8101 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     8161 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     8221 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     8281 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     8341 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     8401 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     8461 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     8521 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     8581 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     8641 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     8701 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     8761 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcac
//
