LOCUS       sequence-226793-        8815 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tet-inducible shRNA targeting OGDH.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8815)
  AUTHORS   Ilic N, Birsoy K, Aguirre AJ, Kory N, Pacold ME, Singh S, Moody SE, 
            DeAngelo JD, Spardy NA, Freinkman E, Weir BA, Tsherniak A, Cowley 
            GS, Root DE, Asara JM, Vazquez F, Widlund HR, Sabatini DM, Hahn WC
  TITLE     PIK3CA mutant tumors depend on oxoglutarate dehydrogenase.
  JOURNAL   Proc Natl Acad Sci U S A. 2017 Apr 25;114(17):E3434-E3443. doi: 
            10.1073/pnas.1617922114. Epub 2017 Apr 10.
  PUBMED    28396387
REFERENCE   2  (bases 1 to 8815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1617922114"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2017-04-25- 25"; volume: "114"; issue: "17"; pages: 
            "E3434-E3443"
COMMENT     Sequence Label: pLKO-puro-tetON-shOGDH 2497
FEATURES             Location/Qualifiers
     source          1..8815
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    226..247
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(262..267,268..285,286..292)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    300..316
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     305..327
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     324..340
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     324..340
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     359..379
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        361..379
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        407..633
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             634..814
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    861..986
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1479..1712
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1897..1941
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2230..2324
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     primer_bind     2241..2260
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    2278..2296
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2306..2324
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    2424..2541
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2590..3100
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3019..3037
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     intron          3109..3681
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     3689..3708
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        3736..3754
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3765..4385
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(3838..3857)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     misc_feature    4420..4993
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4587..4604)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4814..4833
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5013..5612
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5013..5032)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5509..5529
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5740..5973
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6045..6166
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6082..6101)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6136..6155
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6206..6341
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(6362..6380)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6390..6407)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6390..6406)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6399..6421)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6548..7003
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6635..6654)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6845..6866
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7029..7133
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7134..7202,7203..7994)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7352..7371)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8165..8753
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8654..8673
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      241 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      301 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc
      361 aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta gtcttatgca
      421 atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag
      481 aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga
      541 aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat
      601 attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga
      661 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      721 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      781 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      841 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      901 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      961 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
     1021 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
     1081 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
     1141 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1201 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1261 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1321 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     1381 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     1441 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     1501 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     1561 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     1621 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     1681 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     1741 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     1801 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     1861 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     1921 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     1981 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     2041 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     2101 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     2161 gacagatcca ttcgattagt gaacggatct cgacggtatc gatcacgaga ctagcctcga
     2221 gcggccgcaa tatttgcatg tcgctatgtg ttctgggaaa tcaccataaa cgtgaaatcc
     2281 ctatcagtga tagagactta taagttccct atcagtgata gagacaccgg aagccaactt
     2341 cgacatcaat ctcgagattg atgtcgaagt tggcttcttt ttgaattctc gacctcgaga
     2401 caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca
     2461 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa
     2521 attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc actttggccg
     2581 cggctcgagg gggttggggt tgcgcctttt ccaaggcagc cctgggtttg cgcagggacg
     2641 cggctgctct gggcgtggtt ccgggaaacg cagcggcgcc gaccctgggt ctcgcacatt
     2701 cttcacgtcc gttcgcagcg tcacccggat cttcgccgct acccttgtgg gccccccggc
     2761 gacgcttcct gctccgcccc taagtcggga aggttccttg cggttcgcgg cgtgccggac
     2821 gtgacaaacg gaagccgcac gtctcactag taccctcgca gacggacagc gccagggagc
     2881 aatggcagcg cgccgaccgc gatgggctgt ggccaatagc ggctgctcag cagggcgcgc
     2941 cgagagcagc ggccgggaag gggcggtgcg ggaggcgggg tgtggggcgg tagtgtgggc
     3001 cctgttcctg cccgcgcggt gttccgcatt ctgcaagcct ccggagcgca cgtcggcagt
     3061 cggctccctc gttgaccgaa tcaccgacct ctctccccag ggggatctgt gagtttgggg
     3121 acccttgatt gttctttctt tttcgctatt gtaaaattca tgttatatgg agggggcaaa
     3181 gttttcaggg tgttgtttag aatgggaaga tgtcccttgt atcaccatgg accctcatga
     3241 taattttgtt tctttcactt tctactctgt tgacaaccat tgtctcctct tattttcttt
     3301 tcattttctg taactttttc gttaaacttt agcttgcatt tgtaacgaat ttttaaattc
     3361 acttttgttt atttgtcaga ttgtaagtac tttctctaat cacttttttt tcaaggcaat
     3421 cagggtatat tatattgtac ttcagcacag ttttagagaa caattgttat aattaaatga
     3481 taaggtagaa tatttctgca tataaattct ggctggcgtg gaaatattct tattggtaga
     3541 aacaactaca tcctggtcat catcctgcct ttctctttat ggttacaatg atatacactg
     3601 tttgagatga ggataaaata ctctgagtcc aaaccgggcc cctctgctaa ccatgttcat
     3661 gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat
     3721 tttggcaaag aattgtaata cgactcacta tagggcgaat tgatatgtct agattagata
     3781 aaagtaaagt gattaacagc gcattagagc tgcttaatga ggtcggaatc gaaggtttaa
     3841 caacccgtaa actcgcccag aagctaggtg tagagcagcc tacattgtat tggcatgtaa
     3901 aaaataagcg ggctttgctc gacgccttag ccattgagat gttagatagg caccatactc
     3961 acttttgccc tttagaaggg gaaagctggc aagatttttt acgtaataac gctaaaagtt
     4021 ttagatgtgc tttactaagt catcgcgatg gagcaaaagt acatttaggt acacggccta
     4081 cagaaaaaca gtatgaaact ctcgaaaatc aattagcctt tttatgccaa caaggttttt
     4141 cactagagaa tgcattatat gcactcagcg ctgtggggca ttttacttta ggttgcgtat
     4201 tggaagatca agagcatcaa gtcgctaaag aagaaaggga aacacctact actgatagta
     4261 tgccgccatt attacgacaa gctatcgaat tatttgatca ccaaggtgca gagccagcct
     4321 tcttattcgg ccttgaattg atcatatgcg gattagaaaa acaacttaaa tgtgaaagtg
     4381 ggtccgcgta cagcggctcc cgggagttct agggatctgc ccctctccct cccccccccc
     4441 taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt
     4501 ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt
     4561 gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt
     4621 cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct
     4681 ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt
     4741 ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt
     4801 ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa
     4861 ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta
     4921 gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa
     4981 aacacgatga taaggatcca ccggagctta ccatgaccga gtacaagccc acggtgcgcc
     5041 tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact
     5101 accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc
     5161 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg
     5221 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg
     5281 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg
     5341 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg
     5401 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg
     5461 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct
     5521 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca
     5581 cctggtgcat gacccgcaag cccggtgcct gacgcccgcc ccacgacccg cagcgcccga
     5641 ccgaaaggag cgcacgaccc catgcatcgg tacctttaag accaatgact tacaaggcag
     5701 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     5761 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     5821 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     5881 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     5941 gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc
     6001 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag
     6061 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     6121 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct
     6181 agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     6241 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     6301 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg
     6361 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
     6421 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     6481 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     6541 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     6601 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     6661 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     6721 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     6781 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     6841 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     6901 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     6961 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg
     7021 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     7081 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     7141 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     7201 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     7261 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     7321 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     7381 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     7441 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     7501 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     7561 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     7621 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     7681 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     7741 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     7801 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     7861 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     7921 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     7981 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     8041 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     8101 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     8161 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     8221 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     8281 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     8341 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     8401 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     8461 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     8521 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     8581 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     8641 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     8701 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     8761 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgtt
//
