LOCUS       sequence-226804-       15900 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus transfer vector for expression of huntingtin protein in 
            insect and mammalian cellscells as well as in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15900)
  AUTHORS   Harding RJ, Loppnau P, Ackloo S, Lemak A, Hutchinson A, Hunt B, 
            Holehouse AS, Ho JC, Fan L, Toledo-Sherman L, Seitova A, Arrowsmith 
            CH
  TITLE     Design and characterization of mutant and wildtype huntingtin 
            proteins produced from a toolkit of scalable eukaryotic expression 
            systems.
  JOURNAL   J Biol Chem. 2019 Apr 26;294(17):6986-7001. doi: 
            10.1074/jbc.RA118.007204. Epub 2019 Mar 6.
  PUBMED    30842263
REFERENCE   2  (bases 1 to 15900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.007204"; journalName: "J Biol Chem"; date: 
            "2019-04-26- 26"; volume: "294"; issue: "17"; pages: "6986-7001"
COMMENT     Sequence Label: pBacMam2-DiEx-LIC-N-flag_huntingtin_full-lengt...
FEATURES             Location/Qualifiers
     source          1..15900
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      102..556
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(189..208)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     398..419
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        582..686
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(687..755,756..1547)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(905..924)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1718..2306
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2207..2226
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2460..2477
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  2611..2835
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2902..3435)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3624..3629,3630..3646,3647..3652))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     polyA_signal    4160..4208
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     4233..4252
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     enhancer        4313..4692
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4693..4896
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4847..4867
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        4943..5052
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     CDS             5087..5110
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      13989..13998
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             14693..14722
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             14723..14746
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(14836..14856)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(15254..15265)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    15490..15624
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(15527..15546)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     15581..15600
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  15653..15818
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga
       61 ctgaatatca acgcttattt gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
      121 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
      181 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
      241 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
      301 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
      361 ttttcgccct ttgacgttgg agtccacgtt cttaatagtg gactcttgtt ccaaactgga
      421 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
      481 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
      541 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
      601 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
      661 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
      721 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
      781 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
      841 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
      901 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
      961 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     1021 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     1081 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     1141 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     1201 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     1261 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     1321 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     1381 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     1441 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     1501 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     1561 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     1621 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     1681 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     1741 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     1801 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     1861 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     1921 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     1981 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     2041 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     2101 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     2161 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     2221 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     2281 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     2341 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     2401 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     2461 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc
     2521 tgtgcggtat ttcacaccgc atagaccagc cgcgtaacct ggcaaaatcg gttacggttg
     2581 agtaataaat ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa
     2641 caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga
     2701 aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact
     2761 cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg
     2821 gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc
     2881 gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac
     2941 ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc
     3001 ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttcaaga gattgatgag
     3061 cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat
     3121 ctcactacgc ggctgctcaa acttgggcag aacgtaagcc gcgagagcgc caacaaccgc
     3181 ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta
     3241 atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag
     3301 atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat
     3361 gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt
     3421 gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg
     3481 cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga
     3541 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg
     3601 agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc
     3661 gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg
     3721 aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg
     3781 cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg cccttgcttc
     3841 aggagatcgg tagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag
     3901 tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag gactctagct
     3961 atagttctag tggttggcta cgtacccgta gtggctatgg cagggcttgc cgccccgacg
     4021 ttggctgcga gccctgggcc ttcacccgaa cttgggggtt ggggtgggga aaaggaagaa
     4081 acgcgggcgt attggtccca atggggtctc ggtggggtat cgacagagtg ccagccctgg
     4141 gaccgaaccc cgcgtttatg aacaaacgac ccaacacccg tgcgttttat tctgtctttt
     4201 tattgccgtc atagcgcggg ttccttccgg tattgtctcc ttccgtgttt cagttagcct
     4261 cccccatctc ccggtaccgc atgcggatct gtacgggcca gatatacgcg ttgacattga
     4321 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     4381 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     4441 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     4501 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     4561 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     4621 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     4681 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     4741 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     4801 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     4861 aggcgtgtac ggtgggaggt ctatataagc agagctctct ggctaactag agaacccact
     4921 gcttactggc ttatcgaata cggaccttta attcaaccca acacaatata ttatagttaa
     4981 ataagaatta ttatcaaatc atttgtatat taattaaaat actatactgt aaattacatt
     5041 ttatttacaa tcactcgacg gatccttaag aaggagatat actatggact acaaagacga
     5101 tgacgacaag atggcgaccc tggaaaagct gatgaaggcc ttcgagtccc tcaagtcctt
     5161 ccaacagcag caacagcaac aacagcagca acagcaacaa cagcagcaac agcaacaaca
     5221 gcagcaacag caacaacagc agcaacagca acaacagcag caacagcaac aacagcagca
     5281 acagcaacaa cagcagcaac agcaacaaca gcagcaacag caacaacagc agcaacagca
     5341 acaacagcag caacagcaac cgccaccgcc gccgccgccg ccgccgcctc ctcagcttcc
     5401 tcagccgccg ccgcaggcac agccgctgct gcctcagccg cagccgcccc cgccgccgcc
     5461 cccgccgcca cccggcccgg ctgtggctga ggagccgctg caccgaccaa agaaagaact
     5521 ttcagctacc aagaaagacc gtgtgaatca ttgtctgaca atatgtgaaa acatagtggc
     5581 acagtctgtc agaaattctc cagaatttca gaaacttctg ggcatcgcta tggaactttt
     5641 tctgctgtgc agtgatgacg cagagtcaga tgtcaggatg gtggctgacg aatgcctcaa
     5701 caaagttatc aaagctttga tggattctaa tcttccaagg ttacagctcg agctctataa
     5761 ggaaattaaa aagaatggtg cccctcggag tttgcgtgct gccctgtgga ggtttgctga
     5821 gctggctcac ctggttcggc ctcagaaatg caggccttac ctggtgaacc ttctgccgtg
     5881 cctgactcga acaagcaaga gacccgaaga atcagtccag gagaccttgg ctgcagctgt
     5941 tcccaaaatt atggcttctt ttggcaattt tgcaaatgac aatgaaatta aggttttgtt
     6001 aaaggccttc atagcgaacc tgaagtcaag ctcccccacc attcggcgga cagcggctgg
     6061 atcagcagtg agcatctgcc agcactcaag aaggacacaa tatttctata gttggctact
     6121 aaatgtgctc ttaggcttac tcgttcctgt cgaggatgaa cactccactc tgctgattct
     6181 tggcgtgctg ctcaccctga ggtatttggt gcccttgctg cagcagcagg tcaaggacac
     6241 aagcctgaaa ggcagcttcg gagtgacaag gaaagaaatg gaagtctctc cttctgcaga
     6301 gcagcttgtc caggtttatg aactgacgtt acatcataca cagcaccaag accacaatgt
     6361 tgtgaccgga gccctggagc tgttgcagca gctcttcaga acgcctccac ccgagcttct
     6421 gcaaaccctg accgcagtcg ggggcattgg gcagctcacc gctgctaagg aggagtctgg
     6481 tggccgaagc cgtagtggga gtattgtgga acttatagct ggagggggtt cctcatgcag
     6541 ccctgtcctt tcaagaaaac aaaaaggcaa agtgctctta ggagaagaag aagccttgga
     6601 ggatgactct gaatcgagat cggatgtcag cagctctgcc ttaacagcct cagtgaagga
     6661 tgagatcagt ggagagctgg ctgcttcttc aggggtttcc actccagggt cagcaggtca
     6721 tgacatcatc acagaacagc cacggtcaca gcacacactg caggcggact cagtggatct
     6781 ggccagctgt gacttgacaa gctctgccac tgatggggat gaggaggata tcttgagcca
     6841 cagctccagc caggtcagcg ccgtcccatc tgaccctgcc atggacctga atgatgggac
     6901 ccaggcctcg tcgcccatca gcgacagctc ccagaccacc accgaagggc ctgattcagc
     6961 tgttacccct tcagacagtt ctgaaattgt gttagacggt accgacaacc agtatttggg
     7021 cctgcagatt ggacagcccc aggatgaaga tgaggaagcc acaggtattc ttcctgatga
     7081 agcctcggag gccttcagga actcttccat ggcccttcaa caggcacatt tattgaaaaa
     7141 catgagtcac tgcaggcagc cttctgacag cagtgttgat aaatttgtgt tgagagatga
     7201 agctactgaa ccgggtgatc aagaaaacaa gccttgccgc atcaaaggtg acattggaca
     7261 gtccactgat gatgactctg cacctcttgt ccattgtgtc cgccttttat ctgcttcgtt
     7321 tttgctaaca gggggaaaaa atgtgctggt tccggacagg gatgtgaggg tcagcgtgaa
     7381 ggccctggcc ctcagctgtg tgggagcagc tgtggccctc cacccggaat ctttcttcag
     7441 caaactctat aaagttcctc ttgacaccac ggaataccct gaggaacagt atgtctcaga
     7501 catcttgaac tacatcgatc atggagaccc acaggttcga ggagccactg ccattctctg
     7561 tgggaccctc atctgctcca tcctcagcag gtcccgcttc cacgtgggag attggatggg
     7621 caccattaga accctcacag gaaatacatt ttctttggcg gattgcattc ctttgctgcg
     7681 gaaaacactg aaggatgagt cttctgttac ttgcaagtta gcttgtacag ctgtgaggaa
     7741 ctgtgtcatg agtctctgca gcagcagcta cagtgagtta ggactgcagc tgatcatcga
     7801 tgtgctgact ctgaggaaca gttcctattg gctggtgagg acagagcttc tggaaaccct
     7861 tgcagagatt gacttcaggc tggtgagctt tttggaggca aaagcagaaa acttacacag
     7921 aggggctcat cattatacag ggcttttaaa actgcaagaa cgagtgctca ataatgttgt
     7981 catccatttg cttggagatg aagaccccag ggtgcgacat gttgccgcag catcactaat
     8041 taggcttgtc ccaaagctgt tttataaatg tgaccaagga caagctgatc cagtagtggc
     8101 cgtggcaaga gatcaaagca gtgtttacct gaaacttctc atgcatgaga cgcagcctcc
     8161 atctcatttc tccgtcagca caataaccag aatatataga ggctataacc tactaccaag
     8221 cataacagac gtcactatgg aaaataacct ttcaagagtt attgcagcag tttctcatga
     8281 actaatcaca tcaaccacca gagcactcac atttggatgc tgtgaagctt tgtgtcttct
     8341 ttccactgcc ttcccagttt gcatttggag tttaggttgg cactgtggag tgcctccact
     8401 gagtgcctca gatgagtcta ggaagagctg taccgttggg atggccacaa tgattctgac
     8461 cctgctctcg tcagcttggt tcccattgga tctctcagcc catcaagatg ctttgatttt
     8521 ggccggaaac ttgcttgcag ccagtgctcc caaatctctg agaagttcat gggcctctga
     8581 agaagaagcc aacccagcag ccaccaagca agaggaggtc tggccagccc tgggggaccg
     8641 ggccctggtg cccatggtgg agcagctctt ctctcacctg ctgaaggtga ttaacatttg
     8701 tgcccacgtc ctggatgacg tggctcctgg acccgcaata aaggcagcct tgccttctct
     8761 aacaaacccc ccttctctaa gtcccatccg acgaaagggg aaggagaaag aaccaggaga
     8821 acaagcatct gtaccgttga gtcccaagaa aggcagtgag gccagtgcag cttctagaca
     8881 atctgatacc tcaggtcctg ttacaacaag taaatcctca tcactgggga gtttctatca
     8941 tcttccttca tacctcaaac tgcatgatgt cctgaaagct acacacgcta actacaaggt
     9001 cacgctggat cttcagaaca gcacggaaaa gtttggaggg tttcttcgct cagccttgga
     9061 tgttctttct cagatactag agctggccac actgcaggac attgggaagt gtgttgaaga
     9121 gatcctagga tacctgaaat cctgctttag tcgagaacca atgatggcaa ctgtttgtgt
     9181 tcaacaattg ttgaagactc tctttggcac aaacttggcc tcccagtttg atggcttatc
     9241 ttccaacccc agcaagtcac aaggccgagc acagcgcctt ggctcctcca gtgtgaggcc
     9301 aggcttgtac cactactgct tcatggcccc gtacacccac ttcacccagg ccctcgctga
     9361 cgccagcctg aggaacatgg tgcaggcgga gcaggagaac gacacctcgg gatggtttga
     9421 tgtcctccag aaagtgtcta cccagttgaa gacaaacctc acgagtgtca caaagaaccg
     9481 tgcagataag aatgctattc ataatcacat tcgtttgttt gaacctcttg ttataaaagc
     9541 tttaaaacag tacacgacta caacatgtgt gcagttacag aagcaggttt tagatttgct
     9601 ggcgcagctg gttcagttac gggttaatta ctgtcttctg gattcagatc aggtgtttat
     9661 tggctttgta ttgaaacagt ttgaatacat tgaagtgggc cagttcaggg aatcagaggc
     9721 aatcattcca aacatctttt tcttcttggt attactatct tatgaacgct atcattcaaa
     9781 acagatcatt ggaattccta aaatcattca gctctgtgat ggcatcatgg ccagtggaag
     9841 gaaggctgtg acacatgcca taccggctct gcagcccata gtccacgacc tctttgtatt
     9901 aagaggaaca aataaagctg atgcaggaaa agagcttgaa acccaaaaag aggtggtggt
     9961 gtcaatgtta ctgagactca tccagtacca tcaggtgttg gagatgttca ttcttgtcct
    10021 gcagcagtgc cacaaggaga atgaagacaa gtggaagcga ctgtctcgac agatagctga
    10081 catcatcctc ccaatgttag ccaaacagca gatgcacatt gactctcatg aagcccttgg
    10141 agtgttaaat acattatttg agattttggc cccttcctcc ctccgtccgg tagacatgct
    10201 tttacggagt atgttcgtca ctccaaacac aatggcgtcc gtgagcactg ttcaactgtg
    10261 gatatcggga attctggcca ttttgagggt tctgatttcc cagtcaactg aagatattgt
    10321 tctttctcgt attcaggagc tctccttctc tccgtattta atctcctgta cagtaattaa
    10381 taggttaaga gatggggaca gtacttcaac gctagaagaa cacagtgaag ggaaacaaat
    10441 aaagaatttg ccagaagaaa cattttcaag gtttctatta caactggttg gtattctttt
    10501 agaagacatt gttacaaaac agctgaaggt ggaaatgagt gagcagcaac atactttcta
    10561 ttgccaggaa ctaggcacac tgctaatgtg tctgatccac atcttcaagt ctggaatgtt
    10621 ccggagaatc acagcagctg ccactaggct gttccgcagt gatggctgtg gcggcagttt
    10681 ctacaccctg gacagcttga acttgcgggc tcgttccatg atcaccaccc acccggccct
    10741 ggtgctgctc tggtgtcaga tactgctgct tgtcaaccac accgactacc gctggtgggc
    10801 agaagtgcag cagaccccga aaagacacag tctgtccagc acaaagttac ttagtcccca
    10861 gatgtctgga gaagaggagg attctgactt ggcagccaaa cttggaatgt gcaatagaga
    10921 aatagtacga agaggggctc tcattctctt ctgtgattat gtctgtcaga acctccatga
    10981 ctccgagcac ttaacgtggc tcattgtaaa tcacattcaa gatctgatca gcctttccca
    11041 cgagcctcca gtacaggact tcatcagtgc cgttcatcgg aactctgctg ccagcggcct
    11101 gttcatccag gcaattcagt ctcgttgtga aaacctttca actccaacca tgctgaagaa
    11161 aactcttcag tgcttggagg ggatccatct cagccagtcg ggagctgtgc tcacgctgta
    11221 tgtggacagg cttctgtgca cccctttccg tgtgctggct cgcatggtcg acatccttgc
    11281 ttgtcgccgg gtagaaatgc ttctggctgc aaatttacag agcagcatgg cccagttgcc
    11341 aatggaagaa ctcaacagaa tccaggaata ccttcagagc agcgggctcg ctcagagaca
    11401 ccaaaggctc tattccctgc tggacaggtt tcgtctctcc accatgcaag actcacttag
    11461 tccctctcct ccagtctctt cccacccgct ggacggggat gggcacgtgt cactggaaac
    11521 agtgagtccg gacaaagact ggtacgttca tcttgtcaaa tcccagtgtt ggaccaggtc
    11581 agattctgca ctgctggaag gtgcagagct ggtgaatcgg attcctgctg aagatatgaa
    11641 tgccttcatg atgaactcgg agttcaacct aagcctgcta gctccatgct taagcctagg
    11701 gatgagtgaa atttctggtg gccagaagag tgcccttttt gaagcagccc gtgaggtgac
    11761 tctggcccgt gtgagcggca ccgtgcagca gctccctgct gtccatcatg tcttccagcc
    11821 cgagctgcct gcagaaccgg cggcctactg gagcaagttg aatgatctgt ttggggatgc
    11881 tgcactgtat cagtccctgc ccactctggc ccgggccctg gcacagtacc tggtggtggt
    11941 ctccaaactg cccagtcatt tgcaccttcc tcctgagaaa gagaaggaca ttgtgaaatt
    12001 cgtggtggca acccttgagg ccctgtcctg gcatttgatc catgagcaga tcccgctgag
    12061 tctggatctc caggcagggc tggactgctg ctgcctggcc ctgcagctgc ctggcctctg
    12121 gagcgtggtc tcctccacag agtttgtgac ccacgcctgc tccctcatcc actgtgtgca
    12181 cttcatcctg gaggccgttg cagtgcagcc tggagagcag cttcttagtc cagaaagaag
    12241 gacaaatacc ccaaaagcca tcagcgagga ggaggaggaa gtagatccaa acacacagaa
    12301 tcctaagtat atcactgcag cctgtgagat ggtggcagaa atggtggagt ctctgcagtc
    12361 ggtgttggcc ttgggtcata aaaggaatag cggcgtgccg gcgtttctca cgccattgct
    12421 caggaacatc atcatcagcc tggcccgcct gccccttgtc aacagctaca cacgtgtgcc
    12481 cccactggtg tggaagcttg gatggtcacc caaaccggga ggggattttg gcacagcatt
    12541 ccctgagatc cccgtggagt tcctccagga aaaggaagtc tttaaggagt tcatctaccg
    12601 catcaacaca ctaggctgga ccagtcgtac tcagtttgaa gaaacttggg ccaccctcct
    12661 tggtgtcctg gtgacgcagc ccctcgtgat ggagcaggag gagagcccac cagaagaaga
    12721 cacagagagg acccagatca acgtcctggc cgtgcaggcc atcacctcac tggtgctcag
    12781 tgcaatgact gtgcctgtgg ccggcaaccc agctgtaagc tgcttggagc agcagccccg
    12841 gaacaagcct ctgaaagctc tcgacaccag gtttgggagg aagctgagca ttatcagagg
    12901 gattgtggag caagagattc aagcaatggt ttcaaagaga gagaatattg ccacccatca
    12961 tttatatcag gcatgggatc ctgtcccttc tctgtctccg gctactacag gtgccctcat
    13021 cagccacgag aagctgctgc tgcagatcaa ccccgagcgg gagctgggga gcatgagcta
    13081 caaactcggc caggtgtcca tacactccgt gtggctaggg aacagcatca cacccctgag
    13141 ggaggaggaa tgggacgagg aagaggagga ggaggccgac gcccctgcac cttcgtcacc
    13201 acccacgtct ccagtcaact ccaggaaaca ccgggctgga gttgacatcc actcctgttc
    13261 gcagtttttg cttgagttgt acagccgctg gatcctgccg tccagctcag ccaggaggac
    13321 cccggccatc ctgatcagtg aggtggtcag atcccttcta gtggtctcag acttgttcac
    13381 cgagcgcaac cagtttgagc tgatgtatgt gacgctgaca gaactgcgaa gggtgcaccc
    13441 ttcagaagac gagatcctcg ctcagtacct ggtgcctgcc acctgcaagg cagctgccgt
    13501 ccttgggatg gacaaggccg tggcggagcc tgtcagccgc ctgctggaga gcacgctcag
    13561 gagcagccac ctgcccagca gggttggagc cctgcacggc atcctctatg tgctggagtg
    13621 cgacctgctg gacgacactg ccaagcagct catcccggtc atcagcgact atctcctctc
    13681 caacctgaaa gggatcgccc actgcgtgaa cattcacagc cagcagcacg tactggtcat
    13741 gtgtgccact gcgttttacc tcattgagaa ctatcctctg gacgtagggc cggaattttc
    13801 agcatcaata atacagatgt gtggggtgat gctgtctgga agtgaggagt ccaccccctc
    13861 catcatttac cactgtgccc tcagaggcct ggagcgcctc ctgctctctg agcagctctc
    13921 ccgcctggat gcagaatcgc tggtcaagct gagtgtggac agagtgaacg tgcacagccc
    13981 gcaccgggcc atggcggctc tgggcctgat gctcacctgc atgtacacag gaaaggagaa
    14041 agtcagtccg ggtagaactt cagaccctaa tcctgcagcc cccgacagcg agtcagtgat
    14101 tgttgctatg gagcgggtat ctgttctttt tgataggatc aggaaaggct ttccttgtga
    14161 agccagagtg gtggccagga tcctgcccca gtttctagac gacttcttcc caccccagga
    14221 catcatgaac aaagtcatcg gagagtttct gtccaaccag cagccatacc cccagttcat
    14281 ggccaccgtg gtgtataagg tgtttcagac tctgcacagc accgggcagt cgtccatggt
    14341 ccgggactgg gtcatgctgt ccctctccaa cttcacgcag agggccccgg tcgccatggc
    14401 cacgtggagc ctctcctgct tctttgtcag cgcgtccacc agcccgtggg tcgcggcgat
    14461 cctcccacat gtcatcagca ggatgggcaa gctggagcag gtggacgtga accttttctg
    14521 cctggtcgcc acagacttct acagacacca gatagaggag gagctcgacc gcagggcctt
    14581 ccagtctgtg cttgaggtgg ttgcagcccc aggaagccca tatcaccggc tgctgacttg
    14641 tttacgaaat gtccacaagg tcaccacctg ctaaaacctc tacttccaat cgcaccatca
    14701 tcaccatcac catcaccacc atgattacaa ggatgacgac gataagtgag gcggccgcca
    14761 acttgatatc aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac
    14821 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
    14881 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
    14941 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
    15001 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
    15061 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
    15121 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
    15181 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
    15241 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcgggc gcccccctcc
    15301 tcacggcgag cgaagcttgt cgagaagtac tagaggatct ctagagcctg cagtctcgac
    15361 aagcttgtcg agaagtacta gaggatcata atcagccata ccacatttgt agaggtttta
    15421 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
    15481 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
    15541 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
    15601 aatgtatctt atcatgtctg gatctgatca ctgcttgagc ctaggagatc cgaaccagat
    15661 aagtgaaatc tagttccaaa ctattttgtc atttttaatt ttcgtattag cttacgacgc
    15721 tacacccagt tcccatctat tttgtcactc ttccctaaat aatccttaaa aactccattt
    15781 ccacccctcc cagttcccaa ctattttgtc cgcccacagc ggggcatttt tcttcctgtt
    15841 atgtttttaa tcaaacatcc tgccaactcc atgtgacaaa ccgtcatctt cggctacttt
//
