LOCUS       sequence-226806-       16038 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus transfer vector for expression of huntingtin protein in 
            insect and mammalian cellscells as well as in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16038)
  AUTHORS   Harding RJ, Loppnau P, Ackloo S, Lemak A, Hutchinson A, Hunt B, 
            Holehouse AS, Ho JC, Fan L, Toledo-Sherman L, Seitova A, Arrowsmith 
            CH
  TITLE     Design and characterization of mutant and wildtype huntingtin 
            proteins produced from a toolkit of scalable eukaryotic expression 
            systems.
  JOURNAL   J Biol Chem. 2019 Apr 26;294(17):6986-7001. doi: 
            10.1074/jbc.RA118.007204. Epub 2019 Mar 6.
  PUBMED    30842263
REFERENCE   2  (bases 1 to 16038)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.007204"; journalName: "J Biol Chem"; date: 
            "2019-04-26- 26"; volume: "294"; issue: "17"; pages: "6986-7001"
COMMENT     Sequence Label: pBacMam2-DiEx-LIC-C-flag_huntingtin_full-lengt...
FEATURES             Location/Qualifiers
     source          1..16038
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      102..556
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(189..208)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     398..419
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        582..686
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(687..755,756..1547)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(905..924)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1718..2306
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2207..2226
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2460..2477
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  2611..2835
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2902..3435)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3624..3629,3630..3646,3647..3652))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     polyA_signal    4160..4208
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     4233..4252
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     enhancer        4313..4692
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4693..4896
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4847..4867
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        4943..5052
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     regulatory      14091..14100
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             14786..14809
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             14831..14860
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             14861..14884
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(14974..14994)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(15392..15403)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    15628..15762
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(15665..15684)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     15719..15738
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  15791..15956
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga
       61 ctgaatatca acgcttattt gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
      121 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
      181 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
      241 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
      301 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
      361 ttttcgccct ttgacgttgg agtccacgtt cttaatagtg gactcttgtt ccaaactgga
      421 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
      481 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
      541 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
      601 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
      661 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
      721 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
      781 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
      841 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
      901 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
      961 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     1021 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     1081 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     1141 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     1201 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     1261 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     1321 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     1381 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     1441 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     1501 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     1561 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     1621 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     1681 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     1741 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     1801 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     1861 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     1921 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     1981 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     2041 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     2101 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     2161 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     2221 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     2281 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     2341 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     2401 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     2461 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc
     2521 tgtgcggtat ttcacaccgc atagaccagc cgcgtaacct ggcaaaatcg gttacggttg
     2581 agtaataaat ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa
     2641 caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga
     2701 aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact
     2761 cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg
     2821 gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc
     2881 gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac
     2941 ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc
     3001 ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttcaaga gattgatgag
     3061 cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat
     3121 ctcactacgc ggctgctcaa acttgggcag aacgtaagcc gcgagagcgc caacaaccgc
     3181 ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta
     3241 atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag
     3301 atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat
     3361 gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt
     3421 gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg
     3481 cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga
     3541 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg
     3601 agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc
     3661 gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg
     3721 aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg
     3781 cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg cccttgcttc
     3841 aggagatcgg tagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag
     3901 tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag gactctagct
     3961 atagttctag tggttggcta cgtacccgta gtggctatgg cagggcttgc cgccccgacg
     4021 ttggctgcga gccctgggcc ttcacccgaa cttgggggtt ggggtgggga aaaggaagaa
     4081 acgcgggcgt attggtccca atggggtctc ggtggggtat cgacagagtg ccagccctgg
     4141 gaccgaaccc cgcgtttatg aacaaacgac ccaacacccg tgcgttttat tctgtctttt
     4201 tattgccgtc atagcgcggg ttccttccgg tattgtctcc ttccgtgttt cagttagcct
     4261 cccccatctc ccggtaccgc atgcggatct gtacgggcca gatatacgcg ttgacattga
     4321 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     4381 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     4441 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     4501 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     4561 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     4621 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     4681 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     4741 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     4801 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     4861 aggcgtgtac ggtgggaggt ctatataagc agagctctct ggctaactag agaacccact
     4921 gcttactggc ttatcgaata cggaccttta attcaaccca acacaatata ttatagttaa
     4981 ataagaatta ttatcaaatc atttgtatat taattaaaat actatactgt aaattacatt
     5041 ttatttacaa tcactcgacg gatccttaag aaggagatat actatggcga ccctggaaaa
     5101 gctgatgaag gccttcgagt ccctcaagtc cttccaacag cagcaacagc aacaacagca
     5161 gcaacagcaa caacagcagc aacagcaaca acagcagcaa cagcaacaac agcagcaaca
     5221 gcaacaacag cagcaacagc aacaacaaca gcagcaacag caacaacagc agcaacagca
     5281 acaacagcag caacagcaac aacagcagca acagcaacaa cagcagcaac agcaacaaca
     5341 gcagcaacag caacaacagc agcaacagca acaacagcag caacagcaac aacagcagca
     5401 acagcaacaa cagcagcaac agcaacaaca gcagcaacag caacaacagc agcaacagca
     5461 accgccaccg ccgccgccgc cgccgccgcc tcctcagctt cctcagccgc cgccgcaggc
     5521 acagccgctg ctgcctcagc cgcagccgcc cccgccgccg cccccgccgc cacccggccc
     5581 ggctgtggct gaggagccgc tgcaccgacc aaagaaagaa ctttcagcta ccaagaaaga
     5641 ccgtgtgaat cattgtctga caatatgtga aaacatagtg gcacagtctg tcagaaattc
     5701 tccagaattt cagaaacttc tgggcatcgc tatggaactt tttctgctgt gcagtgatga
     5761 cgcagagtca gatgtcagga tggtggctga cgaatgcctc aacaaagtta tcaaagcttt
     5821 gatggattct aatcttccaa ggttacagct cgagctctat aaggaaatta aaaagaatgg
     5881 tgcccctcgg agtttgcgtg ctgccctgtg gaggtttgct gagctggctc acctggttcg
     5941 gcctcagaaa tgcaggcctt acctggtgaa ccttctgccg tgcctgactc gaacaagcaa
     6001 gagacccgaa gaatcagtcc aggagacctt ggctgcagct gttcccaaaa ttatggcttc
     6061 ttttggcaat tttgcaaatg acaatgaaat taaggttttg ttaaaggcct tcatagcgaa
     6121 cctgaagtca agctccccca ccattcggcg gacagcggct ggatcagcag tgagcatctg
     6181 ccagcactca agaaggacac aatatttcta tagttggcta ctaaatgtgc tcttaggctt
     6241 actcgttcct gtcgaggatg aacactccac tctgctgatt cttggcgtgc tgctcaccct
     6301 gaggtatttg gtgcccttgc tgcagcagca ggtcaaggac acaagcctga aaggcagctt
     6361 cggagtgaca aggaaagaaa tggaagtctc tccttctgca gagcagcttg tccaggttta
     6421 tgaactgacg ttacatcata cacagcacca agaccacaat gttgtgaccg gagccctgga
     6481 gctgttgcag cagctcttca gaacgcctcc acccgagctt ctgcaaaccc tgaccgcagt
     6541 cgggggcatt gggcagctca ccgctgctaa ggaggagtct ggtggccgaa gccgtagtgg
     6601 gagtattgtg gaacttatag ctggaggggg ttcctcatgc agccctgtcc tttcaagaaa
     6661 acaaaaaggc aaagtgctct taggagaaga agaagccttg gaggatgact ctgaatcgag
     6721 atcggatgtc agcagctctg ccttaacagc ctcagtgaag gatgagatca gtggagagct
     6781 ggctgcttct tcaggggttt ccactccagg gtcagcaggt catgacatca tcacagaaca
     6841 gccacggtca cagcacacac tgcaggcgga ctcagtggat ctggccagct gtgacttgac
     6901 aagctctgcc actgatgggg atgaggagga tatcttgagc cacagctcca gccaggtcag
     6961 cgccgtccca tctgaccctg ccatggacct gaatgatggg acccaggcct cgtcgcccat
     7021 cagcgacagc tcccagacca ccaccgaagg gcctgattca gctgttaccc cttcagacag
     7081 ttctgaaatt gtgttagacg gtaccgacaa ccagtatttg ggcctgcaga ttggacagcc
     7141 ccaggatgaa gatgaggaag ccacaggtat tcttcctgat gaagcctcgg aggccttcag
     7201 gaactcttcc atggcccttc aacaggcaca tttattgaaa aacatgagtc actgcaggca
     7261 gccttctgac agcagtgttg ataaatttgt gttgagagat gaagctactg aaccgggtga
     7321 tcaagaaaac aagccttgcc gcatcaaagg tgacattgga cagtccactg atgatgactc
     7381 tgcacctctt gtccattgtg tccgcctttt atctgcttcg tttttgctaa cagggggaaa
     7441 aaatgtgctg gttccggaca gggatgtgag ggtcagcgtg aaggccctgg ccctcagctg
     7501 tgtgggagca gctgtggccc tccacccgga atctttcttc agcaaactct ataaagttcc
     7561 tcttgacacc acggaatacc ctgaggaaca gtatgtctca gacatcttga actacatcga
     7621 tcatggagac ccacaggttc gaggagccac tgccattctc tgtgggaccc tcatctgctc
     7681 catcctcagc aggtcccgct tccacgtggg agattggatg ggcaccatta gaaccctcac
     7741 aggaaataca ttttctttgg cggattgcat tcctttgctg cggaaaacac tgaaggatga
     7801 gtcttctgtt acttgcaagt tagcttgtac agctgtgagg aactgtgtca tgagtctctg
     7861 cagcagcagc tacagtgagt taggactgca gctgatcatc gatgtgctga ctctgaggaa
     7921 cagttcctat tggctggtga ggacagagct tctggaaacc cttgcagaga ttgacttcag
     7981 gctggtgagc tttttggagg caaaagcaga aaacttacac agaggggctc atcattatac
     8041 agggctttta aaactgcaag aacgagtgct caataatgtt gtcatccatt tgcttggaga
     8101 tgaagacccc agggtgcgac atgttgccgc agcatcacta attaggcttg tcccaaagct
     8161 gttttataaa tgtgaccaag gacaagctga tccagtagtg gccgtggcaa gagatcaaag
     8221 cagtgtttac ctgaaacttc tcatgcatga gacgcagcct ccatctcatt tctccgtcag
     8281 cacaataacc agaatatata gaggctataa cctactacca agcataacag acgtcactat
     8341 ggaaaataac ctttcaagag ttattgcagc agtttctcat gaactaatca catcaaccac
     8401 cagagcactc acatttggat gctgtgaagc tttgtgtctt ctttccactg ccttcccagt
     8461 ttgcatttgg agtttaggtt ggcactgtgg agtgcctcca ctgagtgcct cagatgagtc
     8521 taggaagagc tgtaccgttg ggatggccac aatgattctg accctgctct cgtcagcttg
     8581 gttcccattg gatctctcag cccatcaaga tgctttgatt ttggccggaa acttgcttgc
     8641 agccagtgct cccaaatctc tgagaagttc atgggcctct gaagaagaag ccaacccagc
     8701 agccaccaag caagaggagg tctggccagc cctgggggac cgggccctgg tgcccatggt
     8761 ggagcagctc ttctctcacc tgctgaaggt gattaacatt tgtgcccacg tcctggatga
     8821 cgtggctcct ggacccgcaa taaaggcagc cttgccttct ctaacaaacc ccccttctct
     8881 aagtcccatc cgacgaaagg ggaaggagaa agaaccagga gaacaagcat ctgtaccgtt
     8941 gagtcccaag aaaggcagtg aggccagtgc agcttctaga caatctgata cctcaggtcc
     9001 tgttacaaca agtaaatcct catcactggg gagtttctat catcttcctt catacctcaa
     9061 actgcatgat gtcctgaaag ctacacacgc taactacaag gtcacgctgg atcttcagaa
     9121 cagcacggaa aagtttggag ggtttcttcg ctcagccttg gatgttcttt ctcagatact
     9181 agagctggcc acactgcagg acattgggaa gtgtgttgaa gagatcctag gatacctgaa
     9241 atcctgcttt agtcgagaac caatgatggc aactgtttgt gttcaacaat tgttgaagac
     9301 tctctttggc acaaacttgg cctcccagtt tgatggctta tcttccaacc ccagcaagtc
     9361 acaaggccga gcacagcgcc ttggctcctc cagtgtgagg ccaggcttgt accactactg
     9421 cttcatggcc ccgtacaccc acttcaccca ggccctcgct gacgccagcc tgaggaacat
     9481 ggtgcaggcg gagcaggaga acgacacctc gggatggttt gatgtcctcc agaaagtgtc
     9541 tacccagttg aagacaaacc tcacgagtgt cacaaagaac cgtgcagata agaatgctat
     9601 tcataatcac attcgtttgt ttgaacctct tgttataaaa gctttaaaac agtacacgac
     9661 tacaacatgt gtgcagttac agaagcaggt tttagatttg ctggcgcagc tggttcagtt
     9721 acgggttaat tactgtcttc tggattcaga tcaggtgttt attggctttg tattgaaaca
     9781 gtttgaatac attgaagtgg gccagttcag ggaatcagag gcaatcattc caaacatctt
     9841 tttcttcttg gtattactat cttatgaacg ctatcattca aaacagatca ttggaattcc
     9901 taaaatcatt cagctctgtg atggcatcat ggccagtgga aggaaggctg tgacacatgc
     9961 cataccggct ctgcagccca tagtccacga cctctttgta ttaagaggaa caaataaagc
    10021 tgatgcagga aaagagcttg aaacccaaaa agaggtggtg gtgtcaatgt tactgagact
    10081 catccagtac catcaggtgt tggagatgtt cattcttgtc ctgcagcagt gccacaagga
    10141 gaatgaagac aagtggaagc gactgtctcg acagatagct gacatcatcc tcccaatgtt
    10201 agccaaacag cagatgcaca ttgactctca tgaagccctt ggagtgttaa atacattatt
    10261 tgagattttg gccccttcct ccctccgtcc ggtagacatg cttttacgga gtatgttcgt
    10321 cactccaaac acaatggcgt ccgtgagcac tgttcaactg tggatatcgg gaattctggc
    10381 cattttgagg gttctgattt cccagtcaac tgaagatatt gttctttctc gtattcagga
    10441 gctctccttc tctccgtatt taatctcctg tacagtaatt aataggttaa gagatgggga
    10501 cagtacttca acgctagaag aacacagtga agggaaacaa ataaagaatt tgccagaaga
    10561 aacattttca aggtttctat tacaactggt tggtattctt ttagaagaca ttgttacaaa
    10621 acagctgaag gtggaaatga gtgagcagca acatactttc tattgccagg aactaggcac
    10681 actgctaatg tgtctgatcc acatcttcaa gtctggaatg ttccggagaa tcacagcagc
    10741 tgccactagg ctgttccgca gtgatggctg tggcggcagt ttctacaccc tggacagctt
    10801 gaacttgcgg gctcgttcca tgatcaccac ccacccggcc ctggtgctgc tctggtgtca
    10861 gatactgctg cttgtcaacc acaccgacta ccgctggtgg gcagaagtgc agcagacccc
    10921 gaaaagacac agtctgtcca gcacaaagtt acttagtccc cagatgtctg gagaagagga
    10981 ggattctgac ttggcagcca aacttggaat gtgcaataga gaaatagtac gaagaggggc
    11041 tctcattctc ttctgtgatt atgtctgtca gaacctccat gactccgagc acttaacgtg
    11101 gctcattgta aatcacattc aagatctgat cagcctttcc cacgagcctc cagtacagga
    11161 cttcatcagt gccgttcatc ggaactctgc tgccagcggc ctgttcatcc aggcaattca
    11221 gtctcgttgt gaaaaccttt caactccaac catgctgaag aaaactcttc agtgcttgga
    11281 ggggatccat ctcagccagt cgggagctgt gctcacgctg tatgtggaca ggcttctgtg
    11341 cacccctttc cgtgtgctgg ctcgcatggt cgacatcctt gcttgtcgcc gggtagaaat
    11401 gcttctggct gcaaatttac agagcagcat ggcccagttg ccaatggaag aactcaacag
    11461 aatccaggaa taccttcaga gcagcgggct cgctcagaga caccaaaggc tctattccct
    11521 gctggacagg tttcgtctct ccaccatgca agactcactt agtccctctc ctccagtctc
    11581 ttcccacccg ctggacgggg atgggcacgt gtcactggaa acagtgagtc cggacaaaga
    11641 ctggtacgtt catcttgtca aatcccagtg ttggaccagg tcagattctg cactgctgga
    11701 aggtgcagag ctggtgaatc ggattcctgc tgaagatatg aatgccttca tgatgaactc
    11761 ggagttcaac ctaagcctgc tagctccatg cttaagccta gggatgagtg aaatttctgg
    11821 tggccagaag agtgcccttt ttgaagcagc ccgtgaggtg actctggccc gtgtgagcgg
    11881 caccgtgcag cagctccctg ctgtccatca tgtcttccag cccgagctgc ctgcagaacc
    11941 ggcggcctac tggagcaagt tgaatgatct gtttggggat gctgcactgt atcagtccct
    12001 gcccactctg gcccgggccc tggcacagta cctggtggtg gtctccaaac tgcccagtca
    12061 tttgcacctt cctcctgaga aagagaagga cattgtgaaa ttcgtggtgg caacccttga
    12121 ggccctgtcc tggcatttga tccatgagca gatcccgctg agtctggatc tccaggcagg
    12181 gctggactgc tgctgcctgg ccctgcagct gcctggcctc tggagcgtgg tctcctccac
    12241 agagtttgtg acccacgcct gctccctcat ccactgtgtg cacttcatcc tggaggccgt
    12301 tgcagtgcag cctggagagc agcttcttag tccagaaaga aggacaaata ccccaaaagc
    12361 catcagcgag gaggaggagg aagtagatcc aaacacacag aatcctaagt atatcactgc
    12421 agcctgtgag atggtggcag aaatggtgga gtctctgcag tcggtgttgg ccttgggtca
    12481 taaaaggaat agcggcgtgc cggcgtttct cacgccattg ctcaggaaca tcatcatcag
    12541 cctggcccgc ctgccccttg tcaacagcta cacacgtgtg cccccactgg tgtggaagct
    12601 tggatggtca cccaaaccgg gaggggattt tggcacagca ttccctgaga tccccgtgga
    12661 gttcctccag gaaaaggaag tctttaagga gttcatctac cgcatcaaca cactaggctg
    12721 gaccagtcgt actcagtttg aagaaacttg ggccaccctc cttggtgtcc tggtgacgca
    12781 gcccctcgtg atggagcagg aggagagccc accagaagaa gacacagaga ggacccagat
    12841 caacgtcctg gccgtgcagg ccatcacctc actggtgctc agtgcaatga ctgtgcctgt
    12901 ggccggcaac ccagctgtaa gctgcttgga gcagcagccc cggaacaagc ctctgaaagc
    12961 tctcgacacc aggtttggga ggaagctgag cattatcaga gggattgtgg agcaagagat
    13021 tcaagcaatg gtttcaaaga gagagaatat tgccacccat catttatatc aggcatggga
    13081 tcctgtccct tctctgtctc cggctactac aggtgccctc atcagccacg agaagctgct
    13141 gctgcagatc aaccccgagc gggagctggg gagcatgagc tacaaactcg gccaggtgtc
    13201 catacactcc gtgtggctag ggaacagcat cacacccctg agggaggagg aatgggacga
    13261 ggaagaggag gaggaggccg acgcccctgc accttcgtca ccacccacgt ctccagtcaa
    13321 ctccaggaaa caccgggctg gagttgacat ccactcctgt tcgcagtttt tgcttgagtt
    13381 gtacagccgc tggatcctgc cgtccagctc agccaggagg accccggcca tcctgatcag
    13441 tgaggtggtc agatcccttc tagtggtctc agacttgttc accgagcgca accagtttga
    13501 gctgatgtat gtgacgctga cagaactgcg aagggtgcac ccttcagaag acgagatcct
    13561 cgctcagtac ctggtgcctg ccacctgcaa ggcagctgcc gtccttggga tggacaaggc
    13621 cgtggcggag cctgtcagcc gcctgctgga gagcacgctc aggagcagcc acctgcccag
    13681 cagggttgga gccctgcacg gcatcctcta tgtgctggag tgcgacctgc tggacgacac
    13741 tgccaagcag ctcatcccgg tcatcagcga ctatctcctc tccaacctga aagggatcgc
    13801 ccactgcgtg aacattcaca gccagcagca cgtactggtc atgtgtgcca ctgcgtttta
    13861 cctcattgag aactatcctc tggacgtagg gccggaattt tcagcatcaa taatacagat
    13921 gtgtggggtg atgctgtctg gaagtgagga gtccaccccc tccatcattt accactgtgc
    13981 cctcagaggc ctggagcgcc tcctgctctc tgagcagctc tcccgcctgg atgcagaatc
    14041 gctggtcaag ctgagtgtgg acagagtgaa cgtgcacagc ccgcaccggg ccatggcggc
    14101 tctgggcctg atgctcacct gcatgtacac aggaaaggag aaagtcagtc cgggtagaac
    14161 ttcagaccct aatcctgcag cccccgacag cgagtcagtg attgttgcta tggagcgggt
    14221 atctgttctt tttgatagga tcaggaaagg ctttccttgt gaagccagag tggtggccag
    14281 gatcctgccc cagtttctag acgacttctt cccaccccag gacatcatga acaaagtcat
    14341 cggagagttt ctgtccaacc agcagccata cccccagttc atggccaccg tggtgtataa
    14401 ggtgtttcag actctgcaca gcaccgggca gtcgtccatg gtccgggact gggtcatgct
    14461 gtccctctcc aacttcacgc agagggcccc ggtcgccatg gccacgtgga gcctctcctg
    14521 cttctttgtc agcgcgtcca ccagcccgtg ggtcgcggcg atcctcccac atgtcatcag
    14581 caggatgggc aagctggagc aggtggacgt gaaccttttc tgcctggtcg ccacagactt
    14641 ctacagacac cagatagagg aggagctcga ccgcagggcc ttccagtctg tgcttgaggt
    14701 ggttgcagcc ccaggaagcc catatcaccg gctgctgact tgtttacgaa atgtccacaa
    14761 ggtcaccacc tgcggtggaa gcggtgacta caaagacgat gacgacaagt aaaacctcta
    14821 cttccaatcg caccatcatc accatcacca tcaccaccat gattacaagg atgacgacga
    14881 taagtgaggc ggccgccaac ttgatatcaa gcttatcgat aatcaacctc tggattacaa
    14941 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
    15001 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
    15061 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
    15121 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
    15181 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
    15241 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
    15301 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
    15361 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
    15421 ccgcgggcgc ccccctcctc acggcgagcg aagcttgtcg agaagtacta gaggatctct
    15481 agagcctgca gtctcgacaa gcttgtcgag aagtactaga ggatcataat cagccatacc
    15541 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
    15601 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
    15661 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
    15721 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tctgatcact gcttgagcct
    15781 aggagatccg aaccagataa gtgaaatcta gttccaaact attttgtcat ttttaatttt
    15841 cgtattagct tacgacgcta cacccagttc ccatctattt tgtcactctt ccctaaataa
    15901 tccttaaaaa ctccatttcc acccctccca gttcccaact attttgtccg cccacagcgg
    15961 ggcatttttc ttcctgttat gtttttaatc aaacatcctg ccaactccat gtgacaaacc
    16021 gtcatcttcg gctacttt
//
