LOCUS       sequence-226808-        4711 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pCMV-RevNL4.3 allows the expression of Rev protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4711)
  AUTHORS   Khoury G, Purcell DFJ
  TITLE     High Throughput In Vitro Assessment of Latency Reversing Agents on 
            HIV Transcription and Splicing.
  JOURNAL   J Vis Exp. 2019 Jan 22;(143). doi: 10.3791/58753.
  PUBMED    30735158
REFERENCE   2  (bases 1 to 4711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Vis 
            Exp."; date: "2019-01-22"; volume: "(143). doi"; pages: " 
            10.3791/58753"
COMMENT     Sequence Label: pCMV-Rev(NL4.3)
FEATURES             Location/Qualifiers
     source          1..4711
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..68
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     60..77
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     61..77
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     84..103
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        84..102
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(117..133)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(117..133)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     enhancer        162..541
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        542..745
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     696..716
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     742..766
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1007..1026
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             1208..1558
                     /codon_start=1
                     /gene="rev"
                     /product="human immunodeficiency virus 1 (HIV-1) Rev 
                     protein"
                     /label=Rev
                     /note="enhances nuclear export of unspliced and 
                     incompletely spliced viral mRNAs"
                     /translation="MAGRSGDSDEELIRTVRLIKLLYQSNPPPNPEGTRQARRNRRRRW
                     RERQRQIHSISERILSTYLGRSAEPVPLQLPPLERLTLDCNEDCGTSGTQGVGSPQILV
                     ESPTVLESGTKE"
     primer_bind     complement(1832..1849)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1838..2045
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(2073..2089)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2073..2089)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2086..2108)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2097..2113
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    2168..2189
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2306..2323)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2477..3065)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2557..2576)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3208..4017)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     3931..3950
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     4009..4028
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     4159..4177
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
       61 gtaaaacgac ggccagtgaa ttgtaatacg actcactata gggcgaattg gggatcgatc
      121 cactagttct agatccgatg tacgggccag atatacgcgt tgacattgat tattgactag
      181 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
      241 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
      301 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
      361 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
      421 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
      481 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
      541 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
      601 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
      661 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
      721 gtgggaggtc tatataagca gagctcgttt agtgaaccgt cagatcgcct ggagacgcca
      781 tccacgctgt tttgacctcc atagaagaca ccgggaccga tccagcctcc gcggccggga
      841 acggtgcatt ggaacgcgga ttccccgtgc caagagtgac gtaagtaccg cctatagagt
      901 ctataggccc acccccttgg cttcttatgc gacggatccc gtaataagct tgaggtgtgg
      961 caggcttgag atctggccat acacttgagt gacaatgaca tccactttgc ctttctctcc
     1021 acaggtgtcc actcccaggt ccaactgcag ctcggttcta tcgattctag actcgaggtc
     1081 cgcggccgcg tggcgcccga acagggactt gaaagcgaaa gtaaagccag aggagatctc
     1141 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggc
     1201 atctcctatg gcaggaagaa gcggagacag cgacgaagag ctcatcagaa cagtcagact
     1261 catcaagctt ctctatcaaa gcaacccacc tcccaatccc gaggggaccc gacaggcccg
     1321 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     1381 gatccttagc acttatctgg gacgatctgc ggagcctgtg cctcttcagc taccaccgct
     1441 tgagagactt actcttgatt gtaacgagga ttgtggaact tctgggacgc agggggtggg
     1501 aagccctcaa atattggtgg aatctcctac agtattggag tcaggaacta aagaatagtg
     1561 ctgttaactt gctcaatgcc acagccatag cagtagctga ggggacagat agggttatag
     1621 aagtattaca agcagcttat agagctattc gccacatacc tagaagaata agacagggct
     1681 tggaaaggat tttgctataa gatgggtggc aagtggtcaa aaagtagtgt gattggatgg
     1741 cctgctgtaa gggaaagaat gagacgagct gagccagcag cagatggggt gggagcagta
     1801 tctcgagtct agactagagc tcgctgatca gcctcgactg tgccttctag ttgccagcca
     1861 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     1921 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     1981 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     2041 ggggaaggcc tcggactagt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     2101 tatccgctca caattccaca caacatacga gccgcggaag cataaagtgt aaagcctggg
     2161 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     2221 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     2281 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     2341 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     2401 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     2461 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     2521 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     2581 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     2641 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     2701 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     2761 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     2821 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     2881 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     2941 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     3001 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     3061 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     3121 gttaagggat tttggtcatg agctgtcgtt gtgtcgtcaa gtcagcgtaa tgctctgcca
     3181 gtgttacaac caattaacca attctgatta gaaaaactca tcgagcatca aatgaaactg
     3241 caatttattc atatcaggat tatcaatacc atatttttga aaaagtcgtt tctgtaatga
     3301 aggagaaaac tcaccaaggc agttccatag gatggcaaga tcctggtatc gatctgcgat
     3361 tccaactcgt ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa taaggttatc
     3421 aagtgagaaa tcaccatgag tgacgactga atctggtgag aatggcaaaa gtttatgcat
     3481 ttctttccag acttgttcaa caggccagcc attacgctcg tcatcaaaat cactcgcatc
     3541 aaccaaacca ttattcattc gtgattgcgc ctgagcgaga cgaaatacgc gatcgctgtt
     3601 aaaaggacaa ttacaaacag gaatcgaatg caaacgtctc aggaacactg ccagcgcatc
     3661 aacaatattt tcacctgaat caggatattc ttctaatacc tggaatgctg tttttccagg
     3721 gatcgcagtg gtgagtaacc atgcatcatc aggagtacgt ataaaatgct tgatggtggg
     3781 aagaggcata aattctgtca gccagtttag tctgaccatc tcatctgtaa catcattggc
     3841 aacgctacct ttgccatgtt tcagaaacaa ctctggcgca tctggcttcc catacaagcg
     3901 atagattgtc gcacctgatt gccctacatt atcgcgagcc catttatacc catataaatc
     3961 agcatccatg ttggaattta atcgtggcct cgacgtttcc cgttgaatat ggctcataac
     4021 accccttgta ttactgttta tgtaagcaga cagttttatt gttcatgatg atatattttt
     4081 atcttgtgca atgtaacatc agagattttg agacacaacg tggctttccc cccccccccc
     4141 atgacattaa cctataaaaa taggcgtatc acgaggccct attttaaatt cgaaagtact
     4201 ggactcgtcg ttttgtcgtt ttgtcgtttc gtcgttttgt cgttttgtcg ttggactcgt
     4261 cgttttgtcg ttttgtcgtt tcgtcgtttt gtcgtttcgt cgttttgtcg ttttgtcgtt
     4321 ggactcgtcg ttttgtcgtt ttgtcgtttc gtcgttttgt cgttttgtcg ttggactcgt
     4381 cgttttgtcg ttttgtcgtt tcgtcgtttt gtcgttttgt cgttggactc gtcgttttgt
     4441 cgttttgtcg ttggactcgt cgttttgtcg ttttgtcgtt tcgtcgtttt gtcgttttgt
     4501 cgttggactc gtcgttttgt cgttttgtcg tttcgtcgtt tcgtcgtttt gtcgttttgt
     4561 cgttggactc gtcgttttgt cgttttgtcg tttcgtcgtt ttgtcgtttt gtcgttggac
     4621 ctgttaacac gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
     4681 gcctcttcgc tattacgcca gctggcgaaa g
//
