LOCUS       sequence-226812-       15948 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus transfer vector for expression of huntingtin protein in 
            insect and mammalian cellscells as well as in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15948)
  AUTHORS   Harding RJ, Loppnau P, Ackloo S, Lemak A, Hutchinson A, Hunt B, 
            Holehouse AS, Ho JC, Fan L, Toledo-Sherman L, Seitova A, Arrowsmith 
            CH
  TITLE     Design and characterization of mutant and wildtype huntingtin 
            proteins produced from a toolkit of scalable eukaryotic expression 
            systems.
  JOURNAL   J Biol Chem. 2019 Apr 26;294(17):6986-7001. doi: 
            10.1074/jbc.RA118.007204. Epub 2019 Mar 6.
  PUBMED    30842263
REFERENCE   2  (bases 1 to 15948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.007204"; journalName: "J Biol Chem"; date: 
            "2019-04-26- 26"; volume: "294"; issue: "17"; pages: "6986-7001"
COMMENT     Sequence Label: pBacMam2-DiEx-LIC-C-flag_huntingtin_full-lengt...
FEATURES             Location/Qualifiers
     source          1..15948
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      102..556
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(189..208)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     398..419
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        582..686
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(687..755,756..1547)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(905..924)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1718..2306
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2207..2226
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2460..2477
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  2611..2835
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2902..3435)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3624..3629,3630..3646,3647..3652))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     polyA_signal    4160..4208
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     4233..4252
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     enhancer        4313..4692
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4693..4896
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4847..4867
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        4943..5052
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     regulatory      14001..14010
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             14696..14719
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             14741..14770
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             14771..14794
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(14884..14904)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(15302..15313)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    15538..15672
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(15575..15594)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     15629..15648
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  15701..15866
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga
       61 ctgaatatca acgcttattt gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
      121 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
      181 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
      241 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
      301 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
      361 ttttcgccct ttgacgttgg agtccacgtt cttaatagtg gactcttgtt ccaaactgga
      421 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
      481 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
      541 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
      601 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
      661 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
      721 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
      781 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
      841 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
      901 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
      961 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     1021 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     1081 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     1141 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     1201 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     1261 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     1321 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     1381 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     1441 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     1501 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     1561 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     1621 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     1681 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     1741 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     1801 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     1861 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     1921 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     1981 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     2041 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     2101 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     2161 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     2221 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     2281 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     2341 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     2401 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     2461 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc
     2521 tgtgcggtat ttcacaccgc atagaccagc cgcgtaacct ggcaaaatcg gttacggttg
     2581 agtaataaat ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa
     2641 caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga
     2701 aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact
     2761 cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg
     2821 gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc
     2881 gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac
     2941 ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc
     3001 ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttcaaga gattgatgag
     3061 cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat
     3121 ctcactacgc ggctgctcaa acttgggcag aacgtaagcc gcgagagcgc caacaaccgc
     3181 ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta
     3241 atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag
     3301 atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat
     3361 gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt
     3421 gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg
     3481 cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga
     3541 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg
     3601 agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc
     3661 gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg
     3721 aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg
     3781 cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg cccttgcttc
     3841 aggagatcgg tagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag
     3901 tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag gactctagct
     3961 atagttctag tggttggcta cgtacccgta gtggctatgg cagggcttgc cgccccgacg
     4021 ttggctgcga gccctgggcc ttcacccgaa cttgggggtt ggggtgggga aaaggaagaa
     4081 acgcgggcgt attggtccca atggggtctc ggtggggtat cgacagagtg ccagccctgg
     4141 gaccgaaccc cgcgtttatg aacaaacgac ccaacacccg tgcgttttat tctgtctttt
     4201 tattgccgtc atagcgcggg ttccttccgg tattgtctcc ttccgtgttt cagttagcct
     4261 cccccatctc ccggtaccgc atgcggatct gtacgggcca gatatacgcg ttgacattga
     4321 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     4381 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     4441 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     4501 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     4561 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     4621 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     4681 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     4741 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     4801 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     4861 aggcgtgtac ggtgggaggt ctatataagc agagctctct ggctaactag agaacccact
     4921 gcttactggc ttatcgaata cggaccttta attcaaccca acacaatata ttatagttaa
     4981 ataagaatta ttatcaaatc atttgtatat taattaaaat actatactgt aaattacatt
     5041 ttatttacaa tcactcgacg gatccttaag aaggagatat actatggcga ccctggaaaa
     5101 gctgatgaag gccttcgagt ccctcaagtc cttccaacag cagcaacagc aacaacagca
     5161 gcaacagcaa caacagcagc aacagcaaca acagcagcaa cagcaacaac agcagcaaca
     5221 gcaacaacag cagcaacagc aacaacagca gcaacagcaa caacagcagc aacagcaaca
     5281 acagcagcaa cagcaacaac agcagcaaca gcaacaacag cagcaacagc aacaacagca
     5341 gcaacagcaa caacagcagc aacagcaaca accgccaccg ccgccgccgc cgccgccgcc
     5401 tcctcagctt cctcagccgc cgccgcaggc acagccgctg ctgcctcagc cgcagccgcc
     5461 cccgccgccg cccccgccgc cacccggccc ggctgtggct gaggagccgc tgcaccgacc
     5521 aaagaaagaa ctttcagcta ccaagaaaga ccgtgtgaat cattgtctga caatatgtga
     5581 aaacatagtg gcacagtctg tcagaaattc tccagaattt cagaaacttc tgggcatcgc
     5641 tatggaactt tttctgctgt gcagtgatga cgcagagtca gatgtcagga tggtggctga
     5701 cgaatgcctc aacaaagtta tcaaagcttt gatggattct aatcttccaa ggttacagct
     5761 cgagctctat aaggaaatta aaaagaatgg tgcccctcgg agtttgcgtg ctgccctgtg
     5821 gaggtttgct gagctggctc acctggttcg gcctcagaaa tgcaggcctt acctggtgaa
     5881 ccttctgccg tgcctgactc gaacaagcaa gagacccgaa gaatcagtcc aggagacctt
     5941 ggctgcagct gttcccaaaa ttatggcttc ttttggcaat tttgcaaatg acaatgaaat
     6001 taaggttttg ttaaaggcct tcatagcgaa cctgaagtca agctccccca ccattcggcg
     6061 gacagcggct ggatcagcag tgagcatctg ccagcactca agaaggacac aatatttcta
     6121 tagttggcta ctaaatgtgc tcttaggctt actcgttcct gtcgaggatg aacactccac
     6181 tctgctgatt cttggcgtgc tgctcaccct gaggtatttg gtgcccttgc tgcagcagca
     6241 ggtcaaggac acaagcctga aaggcagctt cggagtgaca aggaaagaaa tggaagtctc
     6301 tccttctgca gagcagcttg tccaggttta tgaactgacg ttacatcata cacagcacca
     6361 agaccacaat gttgtgaccg gagccctgga gctgttgcag cagctcttca gaacgcctcc
     6421 acccgagctt ctgcaaaccc tgaccgcagt cgggggcatt gggcagctca ccgctgctaa
     6481 ggaggagtct ggtggccgaa gccgtagtgg gagtattgtg gaacttatag ctggaggggg
     6541 ttcctcatgc agccctgtcc tttcaagaaa acaaaaaggc aaagtgctct taggagaaga
     6601 agaagccttg gaggatgact ctgaatcgag atcggatgtc agcagctctg ccttaacagc
     6661 ctcagtgaag gatgagatca gtggagagct ggctgcttct tcaggggttt ccactccagg
     6721 gtcagcaggt catgacatca tcacagaaca gccacggtca cagcacacac tgcaggcgga
     6781 ctcagtggat ctggccagct gtgacttgac aagctctgcc actgatgggg atgaggagga
     6841 tatcttgagc cacagctcca gccaggtcag cgccgtccca tctgaccctg ccatggacct
     6901 gaatgatggg acccaggcct cgtcgcccat cagcgacagc tcccagacca ccaccgaagg
     6961 gcctgattca gctgttaccc cttcagacag ttctgaaatt gtgttagacg gtaccgacaa
     7021 ccagtatttg ggcctgcaga ttggacagcc ccaggatgaa gatgaggaag ccacaggtat
     7081 tcttcctgat gaagcctcgg aggccttcag gaactcttcc atggcccttc aacaggcaca
     7141 tttattgaaa aacatgagtc actgcaggca gccttctgac agcagtgttg ataaatttgt
     7201 gttgagagat gaagctactg aaccgggtga tcaagaaaac aagccttgcc gcatcaaagg
     7261 tgacattgga cagtccactg atgatgactc tgcacctctt gtccattgtg tccgcctttt
     7321 atctgcttcg tttttgctaa cagggggaaa aaatgtgctg gttccggaca gggatgtgag
     7381 ggtcagcgtg aaggccctgg ccctcagctg tgtgggagca gctgtggccc tccacccgga
     7441 atctttcttc agcaaactct ataaagttcc tcttgacacc acggaatacc ctgaggaaca
     7501 gtatgtctca gacatcttga actacatcga tcatggagac ccacaggttc gaggagccac
     7561 tgccattctc tgtgggaccc tcatctgctc catcctcagc aggtcccgct tccacgtggg
     7621 agattggatg ggcaccatta gaaccctcac aggaaataca ttttctttgg cggattgcat
     7681 tcctttgctg cggaaaacac tgaaggatga gtcttctgtt acttgcaagt tagcttgtac
     7741 agctgtgagg aactgtgtca tgagtctctg cagcagcagc tacagtgagt taggactgca
     7801 gctgatcatc gatgtgctga ctctgaggaa cagttcctat tggctggtga ggacagagct
     7861 tctggaaacc cttgcagaga ttgacttcag gctggtgagc tttttggagg caaaagcaga
     7921 aaacttacac agaggggctc atcattatac agggctttta aaactgcaag aacgagtgct
     7981 caataatgtt gtcatccatt tgcttggaga tgaagacccc agggtgcgac atgttgccgc
     8041 agcatcacta attaggcttg tcccaaagct gttttataaa tgtgaccaag gacaagctga
     8101 tccagtagtg gccgtggcaa gagatcaaag cagtgtttac ctgaaacttc tcatgcatga
     8161 gacgcagcct ccatctcatt tctccgtcag cacaataacc agaatatata gaggctataa
     8221 cctactacca agcataacag acgtcactat ggaaaataac ctttcaagag ttattgcagc
     8281 agtttctcat gaactaatca catcaaccac cagagcactc acatttggat gctgtgaagc
     8341 tttgtgtctt ctttccactg ccttcccagt ttgcatttgg agtttaggtt ggcactgtgg
     8401 agtgcctcca ctgagtgcct cagatgagtc taggaagagc tgtaccgttg ggatggccac
     8461 aatgattctg accctgctct cgtcagcttg gttcccattg gatctctcag cccatcaaga
     8521 tgctttgatt ttggccggaa acttgcttgc agccagtgct cccaaatctc tgagaagttc
     8581 atgggcctct gaagaagaag ccaacccagc agccaccaag caagaggagg tctggccagc
     8641 cctgggggac cgggccctgg tgcccatggt ggagcagctc ttctctcacc tgctgaaggt
     8701 gattaacatt tgtgcccacg tcctggatga cgtggctcct ggacccgcaa taaaggcagc
     8761 cttgccttct ctaacaaacc ccccttctct aagtcccatc cgacgaaagg ggaaggagaa
     8821 agaaccagga gaacaagcat ctgtaccgtt gagtcccaag aaaggcagtg aggccagtgc
     8881 agcttctaga caatctgata cctcaggtcc tgttacaaca agtaaatcct catcactggg
     8941 gagtttctat catcttcctt catacctcaa actgcatgat gtcctgaaag ctacacacgc
     9001 taactacaag gtcacgctgg atcttcagaa cagcacggaa aagtttggag ggtttcttcg
     9061 ctcagccttg gatgttcttt ctcagatact agagctggcc acactgcagg acattgggaa
     9121 gtgtgttgaa gagatcctag gatacctgaa atcctgcttt agtcgagaac caatgatggc
     9181 aactgtttgt gttcaacaat tgttgaagac tctctttggc acaaacttgg cctcccagtt
     9241 tgatggctta tcttccaacc ccagcaagtc acaaggccga gcacagcgcc ttggctcctc
     9301 cagtgtgagg ccaggcttgt accactactg cttcatggcc ccgtacaccc acttcaccca
     9361 ggccctcgct gacgccagcc tgaggaacat ggtgcaggcg gagcaggaga acgacacctc
     9421 gggatggttt gatgtcctcc agaaagtgtc tacccagttg aagacaaacc tcacgagtgt
     9481 cacaaagaac cgtgcagata agaatgctat tcataatcac attcgtttgt ttgaacctct
     9541 tgttataaaa gctttaaaac agtacacgac tacaacatgt gtgcagttac agaagcaggt
     9601 tttagatttg ctggcgcagc tggttcagtt acgggttaat tactgtcttc tggattcaga
     9661 tcaggtgttt attggctttg tattgaaaca gtttgaatac attgaagtgg gccagttcag
     9721 ggaatcagag gcaatcattc caaacatctt tttcttcttg gtattactat cttatgaacg
     9781 ctatcattca aaacagatca ttggaattcc taaaatcatt cagctctgtg atggcatcat
     9841 ggccagtgga aggaaggctg tgacacatgc cataccggct ctgcagccca tagtccacga
     9901 cctctttgta ttaagaggaa caaataaagc tgatgcagga aaagagcttg aaacccaaaa
     9961 agaggtggtg gtgtcaatgt tactgagact catccagtac catcaggtgt tggagatgtt
    10021 cattcttgtc ctgcagcagt gccacaagga gaatgaagac aagtggaagc gactgtctcg
    10081 acagatagct gacatcatcc tcccaatgtt agccaaacag cagatgcaca ttgactctca
    10141 tgaagccctt ggagtgttaa atacattatt tgagattttg gccccttcct ccctccgtcc
    10201 ggtagacatg cttttacgga gtatgttcgt cactccaaac acaatggcgt ccgtgagcac
    10261 tgttcaactg tggatatcgg gaattctggc cattttgagg gttctgattt cccagtcaac
    10321 tgaagatatt gttctttctc gtattcagga gctctccttc tctccgtatt taatctcctg
    10381 tacagtaatt aataggttaa gagatgggga cagtacttca acgctagaag aacacagtga
    10441 agggaaacaa ataaagaatt tgccagaaga aacattttca aggtttctat tacaactggt
    10501 tggtattctt ttagaagaca ttgttacaaa acagctgaag gtggaaatga gtgagcagca
    10561 acatactttc tattgccagg aactaggcac actgctaatg tgtctgatcc acatcttcaa
    10621 gtctggaatg ttccggagaa tcacagcagc tgccactagg ctgttccgca gtgatggctg
    10681 tggcggcagt ttctacaccc tggacagctt gaacttgcgg gctcgttcca tgatcaccac
    10741 ccacccggcc ctggtgctgc tctggtgtca gatactgctg cttgtcaacc acaccgacta
    10801 ccgctggtgg gcagaagtgc agcagacccc gaaaagacac agtctgtcca gcacaaagtt
    10861 acttagtccc cagatgtctg gagaagagga ggattctgac ttggcagcca aacttggaat
    10921 gtgcaataga gaaatagtac gaagaggggc tctcattctc ttctgtgatt atgtctgtca
    10981 gaacctccat gactccgagc acttaacgtg gctcattgta aatcacattc aagatctgat
    11041 cagcctttcc cacgagcctc cagtacagga cttcatcagt gccgttcatc ggaactctgc
    11101 tgccagcggc ctgttcatcc aggcaattca gtctcgttgt gaaaaccttt caactccaac
    11161 catgctgaag aaaactcttc agtgcttgga ggggatccat ctcagccagt cgggagctgt
    11221 gctcacgctg tatgtggaca ggcttctgtg cacccctttc cgtgtgctgg ctcgcatggt
    11281 cgacatcctt gcttgtcgcc gggtagaaat gcttctggct gcaaatttac agagcagcat
    11341 ggcccagttg ccaatggaag aactcaacag aatccaggaa taccttcaga gcagcgggct
    11401 cgctcagaga caccaaaggc tctattccct gctggacagg tttcgtctct ccaccatgca
    11461 agactcactt agtccctctc ctccagtctc ttcccacccg ctggacgggg atgggcacgt
    11521 gtcactggaa acagtgagtc cggacaaaga ctggtacgtt catcttgtca aatcccagtg
    11581 ttggaccagg tcagattctg cactgctgga aggtgcagag ctggtgaatc ggattcctgc
    11641 tgaagatatg aatgccttca tgatgaactc ggagttcaac ctaagcctgc tagctccatg
    11701 cttaagccta gggatgagtg aaatttctgg tggccagaag agtgcccttt ttgaagcagc
    11761 ccgtgaggtg actctggccc gtgtgagcgg caccgtgcag cagctccctg ctgtccatca
    11821 tgtcttccag cccgagctgc ctgcagaacc ggcggcctac tggagcaagt tgaatgatct
    11881 gtttggggat gctgcactgt atcagtccct gcccactctg gcccgggccc tggcacagta
    11941 cctggtggtg gtctccaaac tgcccagtca tttgcacctt cctcctgaga aagagaagga
    12001 cattgtgaaa ttcgtggtgg caacccttga ggccctgtcc tggcatttga tccatgagca
    12061 gatcccgctg agtctggatc tccaggcagg gctggactgc tgctgcctgg ccctgcagct
    12121 gcctggcctc tggagcgtgg tctcctccac agagtttgtg acccacgcct gctccctcat
    12181 ccactgtgtg cacttcatcc tggaggccgt tgcagtgcag cctggagagc agcttcttag
    12241 tccagaaaga aggacaaata ccccaaaagc catcagcgag gaggaggagg aagtagatcc
    12301 aaacacacag aatcctaagt atatcactgc agcctgtgag atggtggcag aaatggtgga
    12361 gtctctgcag tcggtgttgg ccttgggtca taaaaggaat agcggcgtgc cggcgtttct
    12421 cacgccattg ctcaggaaca tcatcatcag cctggcccgc ctgccccttg tcaacagcta
    12481 cacacgtgtg cccccactgg tgtggaagct tggatggtca cccaaaccgg gaggggattt
    12541 tggcacagca ttccctgaga tccccgtgga gttcctccag gaaaaggaag tctttaagga
    12601 gttcatctac cgcatcaaca cactaggctg gaccagtcgt actcagtttg aagaaacttg
    12661 ggccaccctc cttggtgtcc tggtgacgca gcccctcgtg atggagcagg aggagagccc
    12721 accagaagaa gacacagaga ggacccagat caacgtcctg gccgtgcagg ccatcacctc
    12781 actggtgctc agtgcaatga ctgtgcctgt ggccggcaac ccagctgtaa gctgcttgga
    12841 gcagcagccc cggaacaagc ctctgaaagc tctcgacacc aggtttggga ggaagctgag
    12901 cattatcaga gggattgtgg agcaagagat tcaagcaatg gtttcaaaga gagagaatat
    12961 tgccacccat catttatatc aggcatggga tcctgtccct tctctgtctc cggctactac
    13021 aggtgccctc atcagccacg agaagctgct gctgcagatc aaccccgagc gggagctggg
    13081 gagcatgagc tacaaactcg gccaggtgtc catacactcc gtgtggctag ggaacagcat
    13141 cacacccctg agggaggagg aatgggacga ggaagaggag gaggaggccg acgcccctgc
    13201 accttcgtca ccacccacgt ctccagtcaa ctccaggaaa caccgggctg gagttgacat
    13261 ccactcctgt tcgcagtttt tgcttgagtt gtacagccgc tggatcctgc cgtccagctc
    13321 agccaggagg accccggcca tcctgatcag tgaggtggtc agatcccttc tagtggtctc
    13381 agacttgttc accgagcgca accagtttga gctgatgtat gtgacgctga cagaactgcg
    13441 aagggtgcac ccttcagaag acgagatcct cgctcagtac ctggtgcctg ccacctgcaa
    13501 ggcagctgcc gtccttggga tggacaaggc cgtggcggag cctgtcagcc gcctgctgga
    13561 gagcacgctc aggagcagcc acctgcccag cagggttgga gccctgcacg gcatcctcta
    13621 tgtgctggag tgcgacctgc tggacgacac tgccaagcag ctcatcccgg tcatcagcga
    13681 ctatctcctc tccaacctga aagggatcgc ccactgcgtg aacattcaca gccagcagca
    13741 cgtactggtc atgtgtgcca ctgcgtttta cctcattgag aactatcctc tggacgtagg
    13801 gccggaattt tcagcatcaa taatacagat gtgtggggtg atgctgtctg gaagtgagga
    13861 gtccaccccc tccatcattt accactgtgc cctcagaggc ctggagcgcc tcctgctctc
    13921 tgagcagctc tcccgcctgg atgcagaatc gctggtcaag ctgagtgtgg acagagtgaa
    13981 cgtgcacagc ccgcaccggg ccatggcggc tctgggcctg atgctcacct gcatgtacac
    14041 aggaaaggag aaagtcagtc cgggtagaac ttcagaccct aatcctgcag cccccgacag
    14101 cgagtcagtg attgttgcta tggagcgggt atctgttctt tttgatagga tcaggaaagg
    14161 ctttccttgt gaagccagag tggtggccag gatcctgccc cagtttctag acgacttctt
    14221 cccaccccag gacatcatga acaaagtcat cggagagttt ctgtccaacc agcagccata
    14281 cccccagttc atggccaccg tggtgtataa ggtgtttcag actctgcaca gcaccgggca
    14341 gtcgtccatg gtccgggact gggtcatgct gtccctctcc aacttcacgc agagggcccc
    14401 ggtcgccatg gccacgtgga gcctctcctg cttctttgtc agcgcgtcca ccagcccgtg
    14461 ggtcgcggcg atcctcccac atgtcatcag caggatgggc aagctggagc aggtggacgt
    14521 gaaccttttc tgcctggtcg ccacagactt ctacagacac cagatagagg aggagctcga
    14581 ccgcagggcc ttccagtctg tgcttgaggt ggttgcagcc ccaggaagcc catatcaccg
    14641 gctgctgact tgtttacgaa atgtccacaa ggtcaccacc tgcggtggaa gcggtgacta
    14701 caaagacgat gacgacaagt aaaacctcta cttccaatcg caccatcatc accatcacca
    14761 tcaccaccat gattacaagg atgacgacga taagtgaggc ggccgccaac ttgatatcaa
    14821 gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
    14881 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
    14941 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
    15001 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
    15061 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
    15121 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
    15181 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
    15241 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
    15301 ttcggccctc aatccagcgg accttccttc ccgcgggcgc ccccctcctc acggcgagcg
    15361 aagcttgtcg agaagtacta gaggatctct agagcctgca gtctcgacaa gcttgtcgag
    15421 aagtactaga ggatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
    15481 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
    15541 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
    15601 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
    15661 catgtctgga tctgatcact gcttgagcct aggagatccg aaccagataa gtgaaatcta
    15721 gttccaaact attttgtcat ttttaatttt cgtattagct tacgacgcta cacccagttc
    15781 ccatctattt tgtcactctt ccctaaataa tccttaaaaa ctccatttcc acccctccca
    15841 gttcccaact attttgtccg cccacagcgg ggcatttttc ttcctgttat gtttttaatc
    15901 aaacatcctg ccaactccat gtgacaaacc gtcatcttcg gctacttt
//
