LOCUS       sequence-226815-        3580 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AtUBI10 promoter Golden Gate Level 0.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3580)
  AUTHORS   Castel B, Tomlinson L, Locci F, Yang Y, Jones JDG
  TITLE     Optimization of T-DNA architecture for Cas9-mediated mutagenesis in 
            Arabidopsis.
  JOURNAL   PLoS One. 2019 Jan 9;14(1):e0204778. doi: 
            10.1371/journal.pone.0204778. eCollection 2019.
  PUBMED    30625150
REFERENCE   2  (bases 1 to 3580)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2019-01-9"; volume: "14(1):e0204778. doi"; pages: " 
            10.1371/journal.pone.0204778. eCollection 2019"
COMMENT     Sequence Label: pICSL12015
FEATURES             Location/Qualifiers
     source          1..3580
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      12..600
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     501..520
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     754..771
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2218..2236)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2304..2408
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2630..3421
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
       61 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
      121 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
      181 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
      241 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
      301 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
      361 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
      421 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
      481 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
      541 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
      601 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
      661 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      721 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      781 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atcactctgt
      841 ggtctcagga ggtcgagctg caggtcaacg gatcaggata ttcttgttta agatgttgaa
      901 ctctatggag gtttgtatga actgatgatc taggaccgga taagttccct tcttcatagc
      961 gaacttattc aaagaatgtt ttgtgtatca ttcttgttac attgttatta atgaaaaaat
     1021 attattggtc attggactga acacgagtgt taaatatgga ccaggcccca aataagatcc
     1081 attgatatat gaattaaata acaagaataa atcgagtcac caaaccactt gcctttttta
     1141 acgagacttg ttcaccaact tgatacaaaa gtcattatcc tatgcaaatc aataatcata
     1201 caaaaatatc caataacact aaaaaattaa aagaaatgga taatttcaca atatgttata
     1261 cgataaagaa gttacttttc caagaaattc actgatttta taagcccact tgcattagat
     1321 aaatggcaaa aaaaaacaaa aaggaaaaga aataaagcac gaagaattct agaaaatacg
     1381 aaatacgctt caatgcagtg ggacccacgg ttcaattatt gccaattttc agctccaccg
     1441 tatatttaaa aaataaaacg ataatgctaa aaaaatataa atcgtaacga tcgttaaatc
     1501 tcaacggctg gatcttatga cgaccgttag aaattgtggt tgtcgacgag tcagtaataa
     1561 acggcgtcaa agtggttgca gccggcacac acgagtcgtg tttatcaact caaagcacaa
     1621 atacttttcc tcaacctaaa aataaggcaa ttagccaaaa acaactttgc gtgtaaacaa
     1681 cgctcaatac acgtgtcatt ttattattag ctattgcttc accgccttag ctttctcgtg
     1741 acctagtcgt cctcgtcttt tcttcttctt cttctataaa acaataccca aagagctctt
     1801 cttcttcaca attcagattt caatttctca aaatcttaaa aactttctct caattctctc
     1861 taccgtgatc aaggtaaatt tctgtgttcc ttattctctc aaaatcttcg attttgtttt
     1921 cgttcgatcc caatttcgta tatgttcttt ggtttagatt ctgttaatct tagatcgaac
     1981 acgattttct gggtttgatc gttagatatc atcttaattc tcgattaggg tttcatagat
     2041 atcatccgat ttgttcaaat aatttgagtt ttgtcgaata attactcttc gatttgtgat
     2101 ttctatctag atctggtgtt agtttctagt ttgtgcgatc gaatttgtcg attaatctga
     2161 gtttttctga ttaacaggaa tgtgagacca cgaagtggct cttcagtgga cgaaagggcc
     2221 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     2281 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     2341 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     2401 ggaagagtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg
     2461 gcgcagtggc ggttttcatg gcttgttatg actgtttttt tttggggtac agtctatgcc
     2521 tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag
     2581 caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatca tgagggaagc
     2641 ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct
     2701 cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc
     2761 acacagcgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg
     2821 agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg
     2881 cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa
     2941 gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc
     3001 agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc
     3061 cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga
     3121 ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg
     3181 aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc
     3241 gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat
     3301 acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga
     3361 tcagttggaa gaatttgtcc attacgtaaa aggcgagatc accaaggtag tcggcaaata
     3421 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     3481 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     3541 gttttcgttc cactgagcgt cagaccccgt agaaaagatc
//
