LOCUS       sequence-226818-        5353 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AtAG promoter Golden Gate Level 0.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5353)
  AUTHORS   Castel B, Tomlinson L, Locci F, Yang Y, Jones JDG
  TITLE     Optimization of T-DNA architecture for Cas9-mediated mutagenesis in 
            Arabidopsis.
  JOURNAL   PLoS One. 2019 Jan 9;14(1):e0204778. doi: 
            10.1371/journal.pone.0204778. eCollection 2019.
  PUBMED    30625150
REFERENCE   2  (bases 1 to 5353)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2019-01-9"; volume: "14(1):e0204778. doi"; pages: " 
            10.1371/journal.pone.0204778. eCollection 2019"
COMMENT     Sequence Label: pICSL12022
FEATURES             Location/Qualifiers
     source          1..5353
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(24..42)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        110..214
                     /gene="bla"
                     /label=AmpR promoter
     CDS             436..1227
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1398..1986
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1887..1906
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2140..2157
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        5293..5339
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
ORIGIN
        1 gtggctcttc agtggacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
       61 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
      121 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      181 ataaatgctt caataatatt gaaaaaggaa gagtatgcgc tcacgcaact ggtccagaac
      241 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg
      301 tttttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
      361 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
      421 acaaagttaa acatcatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
      481 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
      541 tccgcagtgg atggcggcct gaagccacac agcgatattg atttgctggt tacggtgacc
      601 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
      661 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
      721 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
      781 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
      841 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
      901 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
      961 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     1021 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     1081 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     1141 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccatta cgtaaaaggc
     1201 gagatcacca aggtagtcgg caaataactg tcagaccaag tttactcata tatactttag
     1261 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     1321 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     1381 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     1441 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     1501 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     1561 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     1621 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     1681 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     1741 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     1801 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     1861 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     1921 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     1981 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     2041 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     2101 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     2161 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatca
     2221 ctctgtggtc tcaggagctt aatgtacgct taaatctgca ttttgataag taataaaaaa
     2281 taatgataag ggtcaaatcg accacttgca cagttaagtg attctaatac gaaaccttaa
     2341 aagcaaacat cggttctttt gagtcagaac aaatgcaact taatgtgaca catgatgtga
     2401 agaaaaaaca aaagtaatat aagaaaacaa ttaaatagtt aataaaatat ttccttaaag
     2461 ttgtaacaaa taaagaatca ttttatgaaa caatatgaac cctaaataaa ttaaaattcc
     2521 tctgaaacct taaatttatc gagctagtga ttggctgcca actgccatgc tggcaaaatt
     2581 agagtgacat gattggtctg aacatgtcta gggtttcaga catgtgacat gtgtcaacaa
     2641 cccattaaca cattgggtat aaatccaata gacatttgat agtattaaaa ttgtaaccat
     2701 tggattaaat ttaaacgtga tggatgtaac taaatgactt gtccgagtaa catcacaacg
     2761 ttccatactt tccttatttg gaatataatt aaatttacca tttattcttt tttcttgagt
     2821 ttcctgtata tgtacttgta catagatata tatacacaaa tacgtattac aatgacatat
     2881 tatagacttt gatgtctgaa ctctcaacct tctcgatgga gagatcatga ccgtagattt
     2941 ttttggatcg tagaaggcag accaaactct taaactattg gatccgtact aaaaatctca
     3001 ctttcctctc agtacccata atgagagaga aaatgataaa aatccctaac attattctct
     3061 ctctagaaga aaaaaaagat acttcaaaaa gaaagagaaa ttgcataaat ctatctacac
     3121 caaagatgtt gaagcaattc caatgctata cttctatgcc aaatccattt attcagtgat
     3181 cattaatctt tttacttcca agaaatatga acaatttagt atccttataa tttttgtctc
     3241 tatatatgta atatgaacat tgggtattga ccaaatgaga aatctaatat taaatggtca
     3301 aaagtagtaa tatgatgaca tttttgaatt tataaatagg ttccaaatta attcattatg
     3361 acataaaacc ttcttgtcag aagtcaagaa ctgaaactaa caaaacttta taataaatag
     3421 taaaaataca agtgaaaaat aaaaagaaat aatatctgag tgatgacgtg atcaaagatt
     3481 ctttaacaaa gacaacaaat cttacagaca caaaaaccta atcttgcgct caattccaac
     3541 ctctaaaaaa acctcaaaaa tcttataaaa gaaaataaat aaagaaacga aactctgatt
     3601 tcgtagagta cccatgggat atataaaaag aaattagtag gtaaatgaga actaattttg
     3661 attgactgat ttaatttgaa gtcgttgtta gcttttcttg ttttggacat gagaattata
     3721 tatttcagga catgagagtt gacaactgta aacgattaag aaaactgatc ttttaatttt
     3781 caaacaccat ttaatcttga catgttttat gttttggtgg agaagaaagt aatcacgtgg
     3841 gactctctac taataagtat ttggaaattg cgtgtcgaat tagagattac tagtttgagt
     3901 aatgtagttc gaaatgagat tagttatttt aaattttaaa aagagtaatt ttaaggaata
     3961 acaaaaaaga gtccccataa gctaatttgt cttaattacc tccttgtttc attgactatt
     4021 tgaaatcttg aaaattcagt tgaaatttca aatctatgtt tcttttgacc acttctaaac
     4081 taatcttagc tcatatataa ttttccaaaa ctacaaaaat aacactaaca tttaacattc
     4141 tcaagagaaa acaaaaacaa aaacttagat aaccatctaa attgtcctcc tacatgtacg
     4201 tataagttcc attattttct atcactcata taagttaaat tttcatgaaa actcaaaaat
     4261 ctatctagtt tcaccttatt cactctcact taccatcaca tgtgtttgta tcaaatatat
     4321 gatatgatat aattcatgag agagaaagag aggtagagat aagaaaggaa agtaagagaa
     4381 agaagagaag aaaaagagag acacagacat taacaacaat ggaggatgga tgatcacaaa
     4441 acagaagata tgacctcata gtccttcctt actctctccc caatttgttt cccaaaactt
     4501 acttttatag tcataaaaat caagttttta cctattacaa caccagatct ataaatatat
     4561 ctaaatcttc aagtacttgt tagtaaggaa aatagaaagt tataagattt ttttattatt
     4621 ataataacag aaatgagtga agaaagaaca cccaacaaag tgaatcttag ttctacaaaa
     4681 ctgaatctaa aactccacat tagaaaaaaa cctgattgtt tcttatttct tttcatttat
     4741 tatctaactc tcactcagat ctcctttaac tttgtaccat ttccctcact tcatatatct
     4801 atatataaca aattctctct tttttattta agtctaaggg aaaattaata tacacataaa
     4861 gaacaagaaa ttagatctag aaatttgtta ccaataacca caaagtaaat aaaataaaca
     4921 tatcaaacaa atattcccac taatgttagt atgtttatat atatgtgtgg aatatgaagg
     4981 aaaaaagtga aaaataatcc tacccacaag aggatacaag aagaacagaa tcaaatctag
     5041 cttagaagat cgaaaaccct cgtaaaagat ttatgcttta acataaacat aaaaccctaa
     5101 atcggacttt aaaaatcatt taattattaa taaagtaaga acagagcacg agaagaagaa
     5161 gaaacctgtt gttagagtac tcatagagac gaccacggct agagaaaacg atgagtgcga
     5221 cttcagcatc acagagaaca gagagctcgt aagcttgcat gcctgcaggt cgactctaga
     5281 ggatccggta ccgcaagacc cttcctctat ataaggaagt tcatttcatt tggagagtac
     5341 ttgagaccac gaa
//
