LOCUS       sequence-226825-       10475 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GFP^SEC^3xFlag vector with ccdB markers for cloning homology arms.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10475)
  AUTHORS   Dickinson DJ, Pani AM, Heppert JK, Higgins CD, Goldstein B
  TITLE     Streamlined Genome Engineering with a Self-Excising Drug Selection 
            Cassette.
  JOURNAL   Genetics. 2015 Jun 3. pii: genetics.115.178335.
  PUBMED    26044593
REFERENCE   2  (bases 1 to 10475)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2015 Jun 3. pii: genetics.115.178335."
COMMENT     Sequence Label: pDD282
FEATURES             Location/Qualifiers
     source          1..10475
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1..18)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1..17)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10..32)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(226..245)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     345..367
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(405..423)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        491..595
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(596..664,665..1456)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(814..833)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1627..2215
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2116..2135
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2369..2386
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2503..2524
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2539..2544,2545..2562,2563..2569)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2577..2593
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2582..2604
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2601..2617
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2601..2617
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2889..3194
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             complement(3242..3265)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(3269..3292)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(3296..3319)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    3338..3371
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    3724..3748
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(3749..4774)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(3749..4774)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(4777..4801)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      6719..7042
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     CDS             complement(8859..8888)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    8898..8931
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(8949..8969)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(9905..10210)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
ORIGIN
        1 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
       61 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
      121 cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt attttctcct
      181 tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga
      241 tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
      301 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
      361 tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct
      421 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
      481 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
      541 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
      601 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
      661 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
      721 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
      781 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
      841 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
      901 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
      961 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     1021 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     1081 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     1141 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     1201 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     1261 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     1321 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     1381 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     1441 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     1501 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     1561 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     1621 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     1681 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     1741 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     1801 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     1861 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     1921 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     1981 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     2041 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     2101 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     2161 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     2221 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     2281 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     2341 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     2401 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     2461 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
     2521 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
     2581 gagcggataa caatttcaca caggaaacag ctatgaccat ttatcgattt cctaggaacc
     2641 cgaagtatgt caaaaagagg tatgctatga agcagcgtat tacagtgaca gttgacagcg
     2701 acagctatca gttgctcaag gcatatatga tgtcaatatc tccggttcgg taagcacaac
     2761 catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg
     2821 gatggctgag gtcgcccggt ttattgaaat gaacggctct tttgccgacg agaacagggg
     2881 ctggtgaaat gcagtttaag gtttacacct ataaaagaga gagccgttat cgtctgtttg
     2941 tggatgtaca gagtgatatt attgacacgc ccgggcgacg gatggtgatc cccctggcca
     3001 gtgcacgtct gctgtcagat aaagtccccc gtgaacttta cccggtggtg catatcgggg
     3061 atgaaagctg gcgcatgatg accaccgata tggccagtgt gccggtctcc gttatcgggg
     3121 aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt
     3181 tctggggaat ataaatgtca ggctccctta tacacagcca gtctgcaggt cgaactagtc
     3241 tcttgtcatc gtcatccttg taatcacgct tgtcgtcgtc gtccttgtag tcacgcttat
     3301 cgtcatcgtc tttataatca gatccggctc cctgaaaata acttcgtata atgtatgcta
     3361 tacgaagtta ttaaaaattg cggtcataaa ctgaaacgta acatatgata aggtattttg
     3421 tgtgcggttt tttctatgat gtttcaaaaa aatttatcag aagtaaaaaa cataagaagt
     3481 gtacacagaa cattgtgtag attttgggat agaattatta ttaatggggg gagggagcac
     3541 aatttttttc gactttttat tacgaattaa ttctattttt gaatctacac aatttcattg
     3601 ttagaggtga cttaaaagaa gctaaaaaac aaagaaatta agagaagttt tttgataata
     3661 acaaaaatag ggggtgggag cacagggaga aagagcatgt agggatgttg aagagtaatt
     3721 ggacccactt tgtacaagaa agctgggtct attcctttgc cctcggacga gtgctggggc
     3781 gtcggtttcc actatcggcg agtacttcta cacagccatc ggtccagacg gccgcgcttc
     3841 tgcgggcgat ttgtgtacgc ccgacagtcc cggctccgga tcggacgatt gcgtcgcatc
     3901 gaccctgcgc ccaagctgca tcatcgaaat tgccgtcaac caagctctga tagagttggt
     3961 caagaccaat gcggagcata tacgcccgga gccgcggcga tcctgcaagc tccggatgcc
     4021 tccgctcgaa gtagcgcgtc tgctgctcca tacaagccaa ccacggcctc cagaagaaga
     4081 tgttggcgac ctcgtattgg gaatccccga acatcgcctc gctccagtca atgaccgctg
     4141 ttatgcggcc attgtccgtc aggacattgt tggagccgaa atccgcgtgc acgaggtgcc
     4201 ggacttcggg gcagtcctcg gcccaaagca tcagctcatc gagagcctgc gcgacggacg
     4261 cactgacggt gtcgtccatc acagtttgcc agtgatacac atggggatca gcaatcgcgc
     4321 atatgaaatc acgccatgta gtgtattgac cgattccttg cggtccgaat gggccgaacc
     4381 cgctcgtctg gctaagatcg gccgcagcga tcgcatccat ggcctccgcg accggctgca
     4441 gaacagcggg cagttcggtt tcaggcaggt cttgcaacgt gacaccctgt gcacggcggg
     4501 agatgcaata ggtcaggctc tcgctgaatt ccccaatgtc aagcacttcc ggaatcggga
     4561 gcgcggccga tgcaaagtgc cgataaacat aacgatcttt gtagaaacca tcggcgcagc
     4621 tatttacccg caggacatat ccacgccctc ctacatcgaa gctgaaagca cgagattctt
     4681 cgccctccga gagctgcatc aggtcggaga cgctgtcgaa cttttcgatc agaaacttct
     4741 cgacagacgt cgcggtgagt tcaggctttt tcatggagcc tgcttttttg tacaaacttg
     4801 cggcaccgcc tgacatatta ccttaaaatt caaaaattaa tttcagatca gtcataacca
     4861 agaaaacaaa ataaacgtta ggacagaaaa ttcaaattaa ttaaaaatta tgctgaataa
     4921 gaaaattaca tgcaaagcct tattttcctc atagaaacag ttgaataaca tattttttaa
     4981 ttgaaaacat agcaaaattc gtggtaaaat ttgtcaaaac cttggaaaaa atcgaaaaaa
     5041 gaacatttcc cgatgcattt tcctatttct cgttgccgac aacgtataaa cgttgaatac
     5101 tcagtcaacg aatttggctg ctggcttgga ctctcgcacg gaagagacgc gtgtgtagat
     5161 ttacgacgaa agcaaaaatg atcaccaaaa acggaacgtt gagctggacg gaaatagtgg
     5221 taaagtgaca tgattatagt ttgaagattt ctaatttcac aattagagca aatgttgttc
     5281 ggtatttatt ttcaacggta tttatactat tttccacctt tttctagaac attcgagctg
     5341 cttgttgcaa aaggagggcg actcacattc ggtacatgga aaagtagtgt acacaataaa
     5401 gagacccaga tacattttcc gtctgcgtct ctttgcaccc accgggagta ttttcaaacg
     5461 aatgcatcta ggaccttcta gaacattctg taaggctgca gaatgcgggt atataaggaa
     5521 agcgggctca gaggaagcca acacgctttg ttctagtgca tctaaaaaac ttcgaaaata
     5581 tgtcaaatct tctcactgtt catcaaaatc ttccagcact tccagttgac gcgacaagtg
     5641 atgaagttcg caagaacctc atggatatgt tccgtgacag acaagccttc tccgagcaca
     5701 cctggaagat gcttctctca gtgtgccgtt cttgggctgc ctggtgcaag cttaacaacc
     5761 gcaagtggtt cccagccgag ccagaagacg ttcgtgacta cttgctctac ctccaagctc
     5821 gtggattggc ggtgaagacg atccaacaac atctcggaca gctcaatatg ctccatcgcc
     5881 gatctggact tccacgccca tcagattcta atgccgtatc tctcgtaatg agacgtatcc
     5941 gcaaggaaaa tgttgatgct ggagagagag ccaagcaagc cctcgccttc gaaaggtaag
     6001 tttaaatatt aatgtattca atcctaagac ttgacgaaat tttcagaacc gacttcgatc
     6061 aggtccgttc tcttatggaa aattccgatc gttgccagga catccgaaac ctcgccttcc
     6121 tcggaattgc ttacaacaca cttctccgta tcgccgaaat tgcccgcatt cgcgtcaagg
     6181 atattagtcg cacagacgga ggaagaatgc tcatccacat cggacgtaca aaaacgctgg
     6241 tctcgactgc cggagtcgaa aaagcgctct cccttggagt taccaagctc gttgagcgct
     6301 ggatttcggt ttccggagtc gccgatgatc caaacaacta cctgttctgt cgtgttcgca
     6361 agaacggagt tgctgccccg agtgcaacat cacaactctc gactcgagct cttgaaggta
     6421 agtttaaaca acatattagc taagaataag ctgaaaataa attttcagga atcttcgaag
     6481 ctacccaccg tctcatctac ggagccaagg atgattccgg acaacgttac ttggcttggt
     6541 ctggacattc cgcccgtgtc ggagctgccc gtgacatggc tcgcgctgga gtttccatcc
     6601 cagagattat gcaagccgga ggttggacca acgtcaacat tgtgatgaac tatattagaa
     6661 acttggattc tgaaactggc gccatggttc gtcttctcga agacggtgat taagataaat
     6721 gcaaaatcct ttcaagcatt cccttcttct ctatcactct tctttctttt tgtcaaaaaa
     6781 ttctctcgct aatttatttg cttttttaat gttattattt tatgactttt tatagtcact
     6841 gaaaagtttg catctgagtg aagtgaatgc tatcaaaatg tgattctgtc tgatgtactt
     6901 tcacaatctc tcttcaattc tattttgaag tgctttaaac ccgaaaggtt gagaaaaatg
     6961 cgagcgctca aatatttgta ttgtgttcgt tgagtgaccc aacaaaaaga ggaaacttta
     7021 ttgtgccgcc aagaaaaaag tctcactgaa tctcaaatat tttattagaa aacaccaaca
     7081 atttagatat ttctgtatcc acggttggcg ttgacagcct ctgatggtgg agacttgtct
     7141 gggcactggc agtactcggc atcctttcct ggtcttccgt ctggtccagt ctctccctgg
     7201 gttccgtctg gtccttgacg tccttgcttt ccagatggtc cttctggtcc aacggttcct
     7261 ggctctcctg gtggtccgtg aagaccgttc tgtccagctg gtcctgttgg tccttgatct
     7321 cctcttggtc cctttggtcc tggtcttccg attggatgct cggcgtcgcg tcccttctct
     7381 cctggtggtc ctggctctcc gatctttccg atttgaccgg atgatccttg ctctcctgga
     7441 tgtcctggtt gtccatctct tcctgggttt ccattttgtc ctcttggtcc tggaagtcca
     7501 cgtggtcctg gtcttccaag agctcctggt ggtccaactg gtccttgtgg gcaagtgaag
     7561 catccgacag actcgcgttg tggagcgata tcatcagctc caactcctgg aacaccgtca
     7621 agtcctggaa ctccgtcgag tcctggaact cctggaactc cctttggtcc ggatggtcca
     7681 gctgggcact tgttatcagc attgcagttg caggttggtc catttccatt ggctccattt
     7741 ggagtctttg gctggttgaa gactggtgga actgctggtg gttgacctgg tggagcagat
     7801 ggggatccat cagcatgtgg agttggggta gcgttggtct cttcatattg acaacggaca
     7861 cgcttgctag atcttccgat gacaactatg tccttccaca atccattggt agattgctag
     7921 aaaattaaaa attatttaaa attcaaaata taatttaact cactctgaac acattcatct
     7981 cggtatcaag cttctctctg aatccatcaa cctccgagta gatggttgac atggtcatga
     8041 agcataccat aagagtggcg acagtgaccg aagccgtcac ataacacgca agttttacag
     8101 acatctctga aattatcaaa tttaggattt caaaataatt cggccaaaga aatgtaactc
     8161 acctagtctt cgagaagcag agacttgtcg atttcctacc ggattcgtct ccttttatta
     8221 ggaaatcttt ccgcaagttt tcaagttttt tttgtttttt ttttacatta ccgacgagac
     8281 agtgtgcgag acgcagagac gataaaagac tggataagaa cgggagataa ggagtacgga
     8341 tgtaggtgag gaatgtggcc gaaaggacag atgggcttta ttcgggaaga ttggttggga
     8401 aatgaaccag agaagaatga ctgaataaag ttgttgaaac ttggtgagaa tggaagatag
     8461 acaatcgagg gcagctagtt tccttcctcc tctatatatt tttatgtttt cttttaaacc
     8521 ttcctccata tttctgaatt gaatgttgtt gcatattgtg tgtccttatt tcatttccat
     8581 catccttttg tgtgtttccc ccttccacca attcccccca cgtcctaatg aaatgaaagc
     8641 gagaagataa ataaacttgc acattgtgtt cagatgagag gagcggaata cgaattggga
     8701 gacggaaaga gaaaaaaaaa tgtttagatt tggattgaat taatttttta gaaagcaaga
     8761 aaaaaaacac aaagagaata aataaataat aggaaaaaaa acaaatattc agtatttaaa
     8821 atttgaaaat tcaacgacgt tggcgtcgat catccttaga ggtcctcctc ggagataagc
     8881 ttttgctctc cctgaaaata acttcgtata atgtatgcta tacgaagtta ttttaaactt
     8941 acctttccgg attgaaagta cagattctcc ttgtagagct cgtccattcc gtgggtgatt
     9001 ccggcggcgg tgacgaactc gaggaggacc atgtggtcac gcttctcgtt tgggtccttg
     9061 gagagggcgg attgggtgga gaggtagtgg ttgtctggga ggaggactgg tccgtctccg
     9121 attggggtgt tttgttggta gtggtcggcg agttggacgg atccgtcctc gatgttgtga
     9181 cggatctgaa aatttaaatc agattagtta gttagtaaaa tcatgtttaa acttaccttg
     9241 aagttgacct tgattccgtt cttttgcttg tcggccatga tgtagacgtt gtgggagttg
     9301 tagttgtact cgagcttgtg tccgaggatg tttccgtcct ccttgaagtc gattccctga
     9361 aaatttaaat atgtatggtt agttagtacc gaactgttta aacttacctt gagctcgata
     9421 cggttgacga gggtgtctcc ctcgaacttg acctcggcac gggtcttgta gtttccgtcg
     9481 tcctgaaaat ttaaataatc agtagttagt atatatatgt ttaaacttac cttgaagaag
     9541 atggtacgct cttggacgta tccctctggc atggcggact tgaagaagtc gtgacgcttc
     9601 atgtggtctg ggtaacggga gaagcattgg actccgtagc agaaggtggt gacgagggtt
     9661 ggccatggga ctgggagctt tccggtggtg cagatgaact tgagggtgag ctttccgtag
     9721 gtggcgtctc cctctccctc tccggagacg gagaacttgt gtccgttgac gtctccgtcg
     9781 agctcgacga ggattgggac aactccagtg aacaattctt ctcctttact catcgatgct
     9841 cctgaggctc ccgatgctcc ctaggtcgac ctgcagactg gctgtgtata agggagcctg
     9901 acatttatat tccccagaac atcaggttaa tggcgttttt gatgtcattt tcgcggtggc
     9961 tgagatcagc cacttcttcc ccgataacgg agaccggcac actggccata tcggtggtca
    10021 tcatgcgcca gctttcatcc ccgatatgca ccaccgggta aagttcacgg gggactttat
    10081 ctgacagcag acgtgcactg gccaggggga tcaccatccg tcgcccgggc gtgtcaataa
    10141 tatcactctg tacatccaca aacagacgat aacggctctc tcttttatag gtgtaaacct
    10201 taaactgcat ttcaccagcc cctgttctcg tcggcaaaag agccgttcat ttcaataaac
    10261 cgggcgacct cagccatccc ttcctgattt tccgctttcc agcgttcggc acgcagacga
    10321 cgggcttcat tctgcatggt tgtgcttacc gaaccggaga tattgacatc atatatgcct
    10381 tgagcaactg atagctgtcg ctgtcaactg tcactgtaat acgctgcttc atagcatacc
    10441 tctttttgac atacttcggg taactagtgc cggcg
//
