LOCUS       sequence-226860-       10420 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Nfia and blasticidin resistance gene under control of tetO
            sequence..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10420)
  AUTHORS   Canals I, Ginisty A, Quist E, Timmerman R, Fritze J, Miskinyte G, 
            Monni E, Hansen MG, Hidalgo I, Bryder D, Bengzon J, Ahlenius H
  TITLE     Rapid and efficient induction of functional astrocytes from human 
            pluripotent stem cells.
  JOURNAL   Nat Methods. 2018 Sep;15(9):693-696. doi: 10.1038/s41592-018-0103-2.
            Epub 2018 Aug 20.
  PUBMED    30127505
REFERENCE   2  (bases 1 to 10420)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-018-0103-2"; journalName: "Nat Methods"; date: 
            "2018-09"; volume: "15"; issue: "9"; pages: "693-696"
COMMENT     Sequence Label: tetO.Nfia.Blast
FEATURES             Location/Qualifiers
     source          1..10420
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..39
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     36..60
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1693..1746
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1759..2157
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    2196..2784
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2249..2269)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2667..2678)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2787..2803)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2788..2804)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3309..3489
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3515..3532)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3521..3745
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3791..4219
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3878..3897)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4088..4109
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4228..4248)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4233..4562
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4413..4548
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4475..4494
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4610..4657
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4676..5050
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5180..5301
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5217..5236)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5271..5290
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5350..5366)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5350..5366)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5363..5385)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5374..5390
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5398..5404,5405..5422,5423..5428))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5443..5464
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5581..5598)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5752..6340)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5832..5851)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6511..7302,7303..7371))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7134..7153
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7372..7476)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7551..7570)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7742..8121
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8123..8321
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             8339..8519
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8566..8691
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9184..9417
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9602..9646
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9944..10061
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(10118..10136,10137..10159,10160..10178,10179..10201,
                     10202..10220,10221..10243,10244..10262,10263..10285,
                     10286..10304,10305..10327,10328..10346,10347..10369,
                     10370..10388)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
ORIGIN
        1 ggtaggcgtg tacggtggga ggcctatata agcagagctc gtttagtgaa ccgtcagatc
       61 gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga ccgatccagc
      121 ctccgcggcc ccgaattcat gtattctccg ctctgtctca cccaggatga gtttcatcct
      181 ttcattgaag cacttctgcc ccatgtccgc gccttcgcct acacatggtt caacctgcag
      241 gcccgaaagc ggaaatactt caaaaaacat gagaagcgca tgtcgaaaga agaggagagg
      301 gccgtgaagg atgaactgct aagcgagaag cccgaggtca agcagaagtg ggcttcccga
      361 cttctggcca agttacggaa agatatccga cccgagtacc gagaggattt tgttcttaca
      421 gttacaggga aaaaacctcc atgctgtgtt ctttccaacc ctgatcagaa aggcaagatg
      481 cggagaattg actgcctccg ccaggcagat aaagtatgga ggttggacct cgtcatggtg
      541 atcttgttca aaggtattcc gctggaaagt actgatggcg agcgccttgt aaagagtcca
      601 cagtgctcta atccagggct ctgtgtccag ccccatcaca taggggtttc tgtaaaggaa
      661 ctcgatttat atttggcata ctttgtacat gcagcagatt caagtcaatc tgaaagtccc
      721 agccagccaa gtgaagctga cattaaggac cagccagaaa atggacattt gggcttccag
      781 gacagctttg tcacatcagg tgttttcagt gtgactgagc tagtaagagt gtcacaaaca
      841 ccaatagctg caggaaccgg ccccaatttt tctctctctg atttggaaag ttcttcatac
      901 tacagcatga gtccaggagc aatgaggagg tctctgccca gcacatcctc taccagctct
      961 acaaagcgcc tcaagtctgt ggaggatgaa atggacagtc ctggtgaaga accattttac
     1021 acaggccaag ggcgctcccc agggagtggc agccagtcca gtggatggca tgaagtagag
     1081 ccaggcttgc catctccaag cacactgaag aagtctgaga agtctggttt cagcagcccc
     1141 tccccttcgc agacctcctc cctgggaaca gcattcacac agcatcaccg acctgtcatt
     1201 acaggaccca gagcaagtcc acatgcgacg ccatcgactc tccactttcc aacgtcaccc
     1261 atcatccagc agcctgggcc ttacttctca cacccagcca tccgttacca ccctcaggag
     1321 acgctgaaag agtttgtcca acttgtctgt cctgatgctg gtcagcaagc tggacaggtg
     1381 gggttcctca atcccaatgg aagcagtcaa ggcaaggtgc acaacccatt cctccccacc
     1441 ccaatgttgc cgccgccgcc accaccaccg atggccaggc ctgtgcctct gcccatgcca
     1501 gacaccaagc ctccaaccac atcaacagaa ggaggtgcag cctcccccac ctcaccgacc
     1561 tactcgacac ccagcacctc ccccgcaaac cgattcgtca gtgttggacc acgagatcca
     1621 agctttgtaa atatccctca acagacacag tcctggtacc tgggataagg atccatggct
     1681 agcggctccg gcgagggcag gggaagtctt ctaacatgcg gggacgtgga ggaaaatccc
     1741 ggcccagcat gctctagaat ggccaagcct ttgtctcaag aagaatccac cctcattgaa
     1801 agagcaacgg ctacaatcaa cagcatcccc atctctgaag actacagcgt cgccagcgca
     1861 gctctctcta gcgacggccg catcttcact ggtgtcaatg tatatcattt tactggggga
     1921 ccttgtgcag aactcgtggt gctgggcact gctgctgctg cggcagctgg caacctgact
     1981 tgtatcgtcg cgatcggaaa tgagaacagg ggcatcttga gcccctgcgg acggtgccga
     2041 caggtgcttc tcgatctgca tcctgggatc aaagccatag tgaaggacag tgatggacag
     2101 ccgacggcag ttgggattcg tgaattgctg ccctctggtt atgtgtggga gggctaatta
     2161 attaagctag ctaattcgat atcaagctta tcgataatca acctctggat tacaaaattt
     2221 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     2281 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     2341 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     2401 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     2461 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     2521 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     2581 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
     2641 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     2701 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     2761 tctccctttg ggccgcctcc ccgcatcgat accgtcgacc tcgagaccta gaaaaacatg
     2821 gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac
     2881 aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt
     2941 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     3001 ttcactccca acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact
     3061 tccctgattg gcagaactac acaccagggc cagggatcag atatccactg acctttggat
     3121 ggtgctacaa gctagtacca gttgagcaag agaaggtaga agaagccaat gaaggagaga
     3181 acacccgctt gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtat
     3241 tagagtggag gtttgacagc cgcctagcat ttcatcacat ggcccgagag ctgcatccgg
     3301 actgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     3361 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     3421 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     3481 tctctagcag ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag
     3541 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     3601 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     3661 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     3721 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg
     3781 gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     3841 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     3901 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     3961 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     4021 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     4081 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     4141 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     4201 caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     4261 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     4321 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     4381 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     4441 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     4501 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     4561 aaaagctccc gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta
     4621 atcatcggca tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc
     4681 caagttgacc agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt
     4741 ctggaccgac cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt
     4801 ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac
     4861 cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt
     4921 gtccacgaac ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg
     4981 ggggcgggag ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga
     5041 gcaggactga cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     5101 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     5161 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa
     5221 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     5281 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc
     5341 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     5401 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     5461 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     5521 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     5581 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     5641 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     5701 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     5761 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     5821 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     5881 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     5941 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     6001 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     6061 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     6121 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     6181 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     6241 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     6301 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     6361 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     6421 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     6481 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     6541 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     6601 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     6661 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     6721 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     6781 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     6841 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     6901 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     6961 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     7021 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     7081 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     7141 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     7201 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     7261 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     7321 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     7381 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     7441 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     7501 tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc
     7561 tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag
     7621 tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag
     7681 aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt
     7741 tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc
     7801 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     7861 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     7921 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     7981 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
     8041 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
     8101 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
     8161 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     8221 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
     8281 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gcgcgttttg cctgtactgg
     8341 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     8401 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     8461 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     8521 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
     8581 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
     8641 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
     8701 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
     8761 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
     8821 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
     8881 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
     8941 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
     9001 aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga
     9061 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     9121 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     9181 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     9241 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     9301 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     9361 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     9421 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     9481 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     9541 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     9601 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     9661 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     9721 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     9781 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
     9841 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatcggcact
     9901 gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa agaaaagggg
     9961 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
    10021 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
    10081 gcagagatcc agtttggtta gatctcgagt ttaccactcc ctatcagtga tagagaaaag
    10141 tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca
    10201 ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga
    10261 gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt
    10321 ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt
    10381 gatagagaaa agtgaaagtc gagctcggta cccgggtcga
//
