LOCUS       sequence-226915-       15855 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus transfer vector for expression of huntingtin protein in 
            insect and mammalian cellscells as well as in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15855)
  AUTHORS   Harding RJ, Loppnau P, Ackloo S, Lemak A, Hutchinson A, Hunt B, 
            Holehouse AS, Ho JC, Fan L, Toledo-Sherman L, Seitova A, Arrowsmith 
            CH
  TITLE     Design and characterization of mutant and wildtype huntingtin 
            proteins produced from a toolkit of scalable eukaryotic expression 
            systems.
  JOURNAL   J Biol Chem. 2019 Apr 26;294(17):6986-7001. doi: 
            10.1074/jbc.RA118.007204. Epub 2019 Mar 6.
  PUBMED    30842263
REFERENCE   2  (bases 1 to 15855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.007204"; journalName: "J Biol Chem"; date: 
            "2019-04-26- 26"; volume: "294"; issue: "17"; pages: "6986-7001"
COMMENT     Sequence Label: pBacMam2-DiEx-LIC-C-flag_huntingtin_full-lengt...
FEATURES             Location/Qualifiers
     source          1..15855
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      102..556
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(189..208)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     398..419
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        582..686
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(687..755,756..1547)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(905..924)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1718..2306
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2207..2226
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2460..2477
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  2611..2835
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2902..3435)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3624..3629,3630..3646,3647..3652))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     polyA_signal    4160..4208
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     4233..4252
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     enhancer        4313..4692
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4693..4896
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4847..4867
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        4943..5052
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     regulatory      13908..13917
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             14603..14626
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             14648..14677
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             14678..14701
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(14791..14811)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(15209..15220)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    15445..15579
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(15482..15501)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     15536..15555
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  15608..15773
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga
       61 ctgaatatca acgcttattt gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
      121 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
      181 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
      241 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
      301 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
      361 ttttcgccct ttgacgttgg agtccacgtt cttaatagtg gactcttgtt ccaaactgga
      421 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
      481 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
      541 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
      601 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
      661 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
      721 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
      781 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
      841 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
      901 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
      961 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     1021 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     1081 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     1141 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     1201 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     1261 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     1321 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     1381 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     1441 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     1501 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     1561 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     1621 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     1681 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     1741 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     1801 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     1861 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     1921 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     1981 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     2041 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     2101 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     2161 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     2221 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     2281 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     2341 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     2401 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     2461 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc
     2521 tgtgcggtat ttcacaccgc atagaccagc cgcgtaacct ggcaaaatcg gttacggttg
     2581 agtaataaat ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa
     2641 caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga
     2701 aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact
     2761 cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg
     2821 gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc
     2881 gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac
     2941 ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc
     3001 ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttcaaga gattgatgag
     3061 cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat
     3121 ctcactacgc ggctgctcaa acttgggcag aacgtaagcc gcgagagcgc caacaaccgc
     3181 ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta
     3241 atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag
     3301 atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat
     3361 gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt
     3421 gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg
     3481 cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga
     3541 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg
     3601 agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc
     3661 gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg
     3721 aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg
     3781 cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg cccttgcttc
     3841 aggagatcgg tagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag
     3901 tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag gactctagct
     3961 atagttctag tggttggcta cgtacccgta gtggctatgg cagggcttgc cgccccgacg
     4021 ttggctgcga gccctgggcc ttcacccgaa cttgggggtt ggggtgggga aaaggaagaa
     4081 acgcgggcgt attggtccca atggggtctc ggtggggtat cgacagagtg ccagccctgg
     4141 gaccgaaccc cgcgtttatg aacaaacgac ccaacacccg tgcgttttat tctgtctttt
     4201 tattgccgtc atagcgcggg ttccttccgg tattgtctcc ttccgtgttt cagttagcct
     4261 cccccatctc ccggtaccgc atgcggatct gtacgggcca gatatacgcg ttgacattga
     4321 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     4381 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     4441 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     4501 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     4561 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     4621 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     4681 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     4741 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     4801 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     4861 aggcgtgtac ggtgggaggt ctatataagc agagctctct ggctaactag agaacccact
     4921 gcttactggc ttatcgaata cggaccttta attcaaccca acacaatata ttatagttaa
     4981 ataagaatta ttatcaaatc atttgtatat taattaaaat actatactgt aaattacatt
     5041 ttatttacaa tcactcgacg gatccttaag aaggagatat actatggcga ccctggaaaa
     5101 gctgatgaag gccttcgagt ccctcaagtc cttccaacag cagcaacagc aacaacagca
     5161 gcaacagcaa caacagcagc aacagcaaca acagcagcaa cagcaacaac agcagcaaca
     5221 gcaacaacag cagcaacagc aacaacagca gcaacagcaa caacagcagc aacagcaacc
     5281 gccaccgccg ccgccgccgc cgccgcctcc tcagcttcct cagccgccgc cgcaggcaca
     5341 gccgctgctg cctcagccgc agccgccccc gccgccgccc ccgccgccac ccggcccggc
     5401 tgtggctgag gagccgctgc accgaccaaa gaaagaactt tcagctacca agaaagaccg
     5461 tgtgaatcat tgtctgacaa tatgtgaaaa catagtggca cagtctgtca gaaattctcc
     5521 agaatttcag aaacttctgg gcatcgctat ggaacttttt ctgctgtgca gtgatgacgc
     5581 agagtcagat gtcaggatgg tggctgacga atgcctcaac aaagttatca aagctttgat
     5641 ggattctaat cttccaaggt tacagctcga gctctataag gaaattaaaa agaatggtgc
     5701 ccctcggagt ttgcgtgctg ccctgtggag gtttgctgag ctggctcacc tggttcggcc
     5761 tcagaaatgc aggccttacc tggtgaacct tctgccgtgc ctgactcgaa caagcaagag
     5821 acccgaagaa tcagtccagg agaccttggc tgcagctgtt cccaaaatta tggcttcttt
     5881 tggcaatttt gcaaatgaca atgaaattaa ggttttgtta aaggccttca tagcgaacct
     5941 gaagtcaagc tcccccacca ttcggcggac agcggctgga tcagcagtga gcatctgcca
     6001 gcactcaaga aggacacaat atttctatag ttggctacta aatgtgctct taggcttact
     6061 cgttcctgtc gaggatgaac actccactct gctgattctt ggcgtgctgc tcaccctgag
     6121 gtatttggtg cccttgctgc agcagcaggt caaggacaca agcctgaaag gcagcttcgg
     6181 agtgacaagg aaagaaatgg aagtctctcc ttctgcagag cagcttgtcc aggtttatga
     6241 actgacgtta catcatacac agcaccaaga ccacaatgtt gtgaccggag ccctggagct
     6301 gttgcagcag ctcttcagaa cgcctccacc cgagcttctg caaaccctga ccgcagtcgg
     6361 gggcattggg cagctcaccg ctgctaagga ggagtctggt ggccgaagcc gtagtgggag
     6421 tattgtggaa cttatagctg gagggggttc ctcatgcagc cctgtccttt caagaaaaca
     6481 aaaaggcaaa gtgctcttag gagaagaaga agccttggag gatgactctg aatcgagatc
     6541 ggatgtcagc agctctgcct taacagcctc agtgaaggat gagatcagtg gagagctggc
     6601 tgcttcttca ggggtttcca ctccagggtc agcaggtcat gacatcatca cagaacagcc
     6661 acggtcacag cacacactgc aggcggactc agtggatctg gccagctgtg acttgacaag
     6721 ctctgccact gatggggatg aggaggatat cttgagccac agctccagcc aggtcagcgc
     6781 cgtcccatct gaccctgcca tggacctgaa tgatgggacc caggcctcgt cgcccatcag
     6841 cgacagctcc cagaccacca ccgaagggcc tgattcagct gttacccctt cagacagttc
     6901 tgaaattgtg ttagacggta ccgacaacca gtatttgggc ctgcagattg gacagcccca
     6961 ggatgaagat gaggaagcca caggtattct tcctgatgaa gcctcggagg ccttcaggaa
     7021 ctcttccatg gcccttcaac aggcacattt attgaaaaac atgagtcact gcaggcagcc
     7081 ttctgacagc agtgttgata aatttgtgtt gagagatgaa gctactgaac cgggtgatca
     7141 agaaaacaag ccttgccgca tcaaaggtga cattggacag tccactgatg atgactctgc
     7201 acctcttgtc cattgtgtcc gccttttatc tgcttcgttt ttgctaacag ggggaaaaaa
     7261 tgtgctggtt ccggacaggg atgtgagggt cagcgtgaag gccctggccc tcagctgtgt
     7321 gggagcagct gtggccctcc acccggaatc tttcttcagc aaactctata aagttcctct
     7381 tgacaccacg gaataccctg aggaacagta tgtctcagac atcttgaact acatcgatca
     7441 tggagaccca caggttcgag gagccactgc cattctctgt gggaccctca tctgctccat
     7501 cctcagcagg tcccgcttcc acgtgggaga ttggatgggc accattagaa ccctcacagg
     7561 aaatacattt tctttggcgg attgcattcc tttgctgcgg aaaacactga aggatgagtc
     7621 ttctgttact tgcaagttag cttgtacagc tgtgaggaac tgtgtcatga gtctctgcag
     7681 cagcagctac agtgagttag gactgcagct gatcatcgat gtgctgactc tgaggaacag
     7741 ttcctattgg ctggtgagga cagagcttct ggaaaccctt gcagagattg acttcaggct
     7801 ggtgagcttt ttggaggcaa aagcagaaaa cttacacaga ggggctcatc attatacagg
     7861 gcttttaaaa ctgcaagaac gagtgctcaa taatgttgtc atccatttgc ttggagatga
     7921 agaccccagg gtgcgacatg ttgccgcagc atcactaatt aggcttgtcc caaagctgtt
     7981 ttataaatgt gaccaaggac aagctgatcc agtagtggcc gtggcaagag atcaaagcag
     8041 tgtttacctg aaacttctca tgcatgagac gcagcctcca tctcatttct ccgtcagcac
     8101 aataaccaga atatatagag gctataacct actaccaagc ataacagacg tcactatgga
     8161 aaataacctt tcaagagtta ttgcagcagt ttctcatgaa ctaatcacat caaccaccag
     8221 agcactcaca tttggatgct gtgaagcttt gtgtcttctt tccactgcct tcccagtttg
     8281 catttggagt ttaggttggc actgtggagt gcctccactg agtgcctcag atgagtctag
     8341 gaagagctgt accgttggga tggccacaat gattctgacc ctgctctcgt cagcttggtt
     8401 cccattggat ctctcagccc atcaagatgc tttgattttg gccggaaact tgcttgcagc
     8461 cagtgctccc aaatctctga gaagttcatg ggcctctgaa gaagaagcca acccagcagc
     8521 caccaagcaa gaggaggtct ggccagccct gggggaccgg gccctggtgc ccatggtgga
     8581 gcagctcttc tctcacctgc tgaaggtgat taacatttgt gcccacgtcc tggatgacgt
     8641 ggctcctgga cccgcaataa aggcagcctt gccttctcta acaaaccccc cttctctaag
     8701 tcccatccga cgaaagggga aggagaaaga accaggagaa caagcatctg taccgttgag
     8761 tcccaagaaa ggcagtgagg ccagtgcagc ttctagacaa tctgatacct caggtcctgt
     8821 tacaacaagt aaatcctcat cactggggag tttctatcat cttccttcat acctcaaact
     8881 gcatgatgtc ctgaaagcta cacacgctaa ctacaaggtc acgctggatc ttcagaacag
     8941 cacggaaaag tttggagggt ttcttcgctc agccttggat gttctttctc agatactaga
     9001 gctggccaca ctgcaggaca ttgggaagtg tgttgaagag atcctaggat acctgaaatc
     9061 ctgctttagt cgagaaccaa tgatggcaac tgtttgtgtt caacaattgt tgaagactct
     9121 ctttggcaca aacttggcct cccagtttga tggcttatct tccaacccca gcaagtcaca
     9181 aggccgagca cagcgccttg gctcctccag tgtgaggcca ggcttgtacc actactgctt
     9241 catggccccg tacacccact tcacccaggc cctcgctgac gccagcctga ggaacatggt
     9301 gcaggcggag caggagaacg acacctcggg atggtttgat gtcctccaga aagtgtctac
     9361 ccagttgaag acaaacctca cgagtgtcac aaagaaccgt gcagataaga atgctattca
     9421 taatcacatt cgtttgtttg aacctcttgt tataaaagct ttaaaacagt acacgactac
     9481 aacatgtgtg cagttacaga agcaggtttt agatttgctg gcgcagctgg ttcagttacg
     9541 ggttaattac tgtcttctgg attcagatca ggtgtttatt ggctttgtat tgaaacagtt
     9601 tgaatacatt gaagtgggcc agttcaggga atcagaggca atcattccaa acatcttttt
     9661 cttcttggta ttactatctt atgaacgcta tcattcaaaa cagatcattg gaattcctaa
     9721 aatcattcag ctctgtgatg gcatcatggc cagtggaagg aaggctgtga cacatgccat
     9781 accggctctg cagcccatag tccacgacct ctttgtatta agaggaacaa ataaagctga
     9841 tgcaggaaaa gagcttgaaa cccaaaaaga ggtggtggtg tcaatgttac tgagactcat
     9901 ccagtaccat caggtgttgg agatgttcat tcttgtcctg cagcagtgcc acaaggagaa
     9961 tgaagacaag tggaagcgac tgtctcgaca gatagctgac atcatcctcc caatgttagc
    10021 caaacagcag atgcacattg actctcatga agcccttgga gtgttaaata cattatttga
    10081 gattttggcc ccttcctccc tccgtccggt agacatgctt ttacggagta tgttcgtcac
    10141 tccaaacaca atggcgtccg tgagcactgt tcaactgtgg atatcgggaa ttctggccat
    10201 tttgagggtt ctgatttccc agtcaactga agatattgtt ctttctcgta ttcaggagct
    10261 ctccttctct ccgtatttaa tctcctgtac agtaattaat aggttaagag atggggacag
    10321 tacttcaacg ctagaagaac acagtgaagg gaaacaaata aagaatttgc cagaagaaac
    10381 attttcaagg tttctattac aactggttgg tattctttta gaagacattg ttacaaaaca
    10441 gctgaaggtg gaaatgagtg agcagcaaca tactttctat tgccaggaac taggcacact
    10501 gctaatgtgt ctgatccaca tcttcaagtc tggaatgttc cggagaatca cagcagctgc
    10561 cactaggctg ttccgcagtg atggctgtgg cggcagtttc tacaccctgg acagcttgaa
    10621 cttgcgggct cgttccatga tcaccaccca cccggccctg gtgctgctct ggtgtcagat
    10681 actgctgctt gtcaaccaca ccgactaccg ctggtgggca gaagtgcagc agaccccgaa
    10741 aagacacagt ctgtccagca caaagttact tagtccccag atgtctggag aagaggagga
    10801 ttctgacttg gcagccaaac ttggaatgtg caatagagaa atagtacgaa gaggggctct
    10861 cattctcttc tgtgattatg tctgtcagaa cctccatgac tccgagcact taacgtggct
    10921 cattgtaaat cacattcaag atctgatcag cctttcccac gagcctccag tacaggactt
    10981 catcagtgcc gttcatcgga actctgctgc cagcggcctg ttcatccagg caattcagtc
    11041 tcgttgtgaa aacctttcaa ctccaaccat gctgaagaaa actcttcagt gcttggaggg
    11101 gatccatctc agccagtcgg gagctgtgct cacgctgtat gtggacaggc ttctgtgcac
    11161 ccctttccgt gtgctggctc gcatggtcga catccttgct tgtcgccggg tagaaatgct
    11221 tctggctgca aatttacaga gcagcatggc ccagttgcca atggaagaac tcaacagaat
    11281 ccaggaatac cttcagagca gcgggctcgc tcagagacac caaaggctct attccctgct
    11341 ggacaggttt cgtctctcca ccatgcaaga ctcacttagt ccctctcctc cagtctcttc
    11401 ccacccgctg gacggggatg ggcacgtgtc actggaaaca gtgagtccgg acaaagactg
    11461 gtacgttcat cttgtcaaat cccagtgttg gaccaggtca gattctgcac tgctggaagg
    11521 tgcagagctg gtgaatcgga ttcctgctga agatatgaat gccttcatga tgaactcgga
    11581 gttcaaccta agcctgctag ctccatgctt aagcctaggg atgagtgaaa tttctggtgg
    11641 ccagaagagt gccctttttg aagcagcccg tgaggtgact ctggcccgtg tgagcggcac
    11701 cgtgcagcag ctccctgctg tccatcatgt cttccagccc gagctgcctg cagaaccggc
    11761 ggcctactgg agcaagttga atgatctgtt tggggatgct gcactgtatc agtccctgcc
    11821 cactctggcc cgggccctgg cacagtacct ggtggtggtc tccaaactgc ccagtcattt
    11881 gcaccttcct cctgagaaag agaaggacat tgtgaaattc gtggtggcaa cccttgaggc
    11941 cctgtcctgg catttgatcc atgagcagat cccgctgagt ctggatctcc aggcagggct
    12001 ggactgctgc tgcctggccc tgcagctgcc tggcctctgg agcgtggtct cctccacaga
    12061 gtttgtgacc cacgcctgct ccctcatcca ctgtgtgcac ttcatcctgg aggccgttgc
    12121 agtgcagcct ggagagcagc ttcttagtcc agaaagaagg acaaataccc caaaagccat
    12181 cagcgaggag gaggaggaag tagatccaaa cacacagaat cctaagtata tcactgcagc
    12241 ctgtgagatg gtggcagaaa tggtggagtc tctgcagtcg gtgttggcct tgggtcataa
    12301 aaggaatagc ggcgtgccgg cgtttctcac gccattgctc aggaacatca tcatcagcct
    12361 ggcccgcctg ccccttgtca acagctacac acgtgtgccc ccactggtgt ggaagcttgg
    12421 atggtcaccc aaaccgggag gggattttgg cacagcattc cctgagatcc ccgtggagtt
    12481 cctccaggaa aaggaagtct ttaaggagtt catctaccgc atcaacacac taggctggac
    12541 cagtcgtact cagtttgaag aaacttgggc caccctcctt ggtgtcctgg tgacgcagcc
    12601 cctcgtgatg gagcaggagg agagcccacc agaagaagac acagagagga cccagatcaa
    12661 cgtcctggcc gtgcaggcca tcacctcact ggtgctcagt gcaatgactg tgcctgtggc
    12721 cggcaaccca gctgtaagct gcttggagca gcagccccgg aacaagcctc tgaaagctct
    12781 cgacaccagg tttgggagga agctgagcat tatcagaggg attgtggagc aagagattca
    12841 agcaatggtt tcaaagagag agaatattgc cacccatcat ttatatcagg catgggatcc
    12901 tgtcccttct ctgtctccgg ctactacagg tgccctcatc agccacgaga agctgctgct
    12961 gcagatcaac cccgagcggg agctggggag catgagctac aaactcggcc aggtgtccat
    13021 acactccgtg tggctaggga acagcatcac acccctgagg gaggaggaat gggacgagga
    13081 agaggaggag gaggccgacg cccctgcacc ttcgtcacca cccacgtctc cagtcaactc
    13141 caggaaacac cgggctggag ttgacatcca ctcctgttcg cagtttttgc ttgagttgta
    13201 cagccgctgg atcctgccgt ccagctcagc caggaggacc ccggccatcc tgatcagtga
    13261 ggtggtcaga tcccttctag tggtctcaga cttgttcacc gagcgcaacc agtttgagct
    13321 gatgtatgtg acgctgacag aactgcgaag ggtgcaccct tcagaagacg agatcctcgc
    13381 tcagtacctg gtgcctgcca cctgcaaggc agctgccgtc cttgggatgg acaaggccgt
    13441 ggcggagcct gtcagccgcc tgctggagag cacgctcagg agcagccacc tgcccagcag
    13501 ggttggagcc ctgcacggca tcctctatgt gctggagtgc gacctgctgg acgacactgc
    13561 caagcagctc atcccggtca tcagcgacta tctcctctcc aacctgaaag ggatcgccca
    13621 ctgcgtgaac attcacagcc agcagcacgt actggtcatg tgtgccactg cgttttacct
    13681 cattgagaac tatcctctgg acgtagggcc ggaattttca gcatcaataa tacagatgtg
    13741 tggggtgatg ctgtctggaa gtgaggagtc caccccctcc atcatttacc actgtgccct
    13801 cagaggcctg gagcgcctcc tgctctctga gcagctctcc cgcctggatg cagaatcgct
    13861 ggtcaagctg agtgtggaca gagtgaacgt gcacagcccg caccgggcca tggcggctct
    13921 gggcctgatg ctcacctgca tgtacacagg aaaggagaaa gtcagtccgg gtagaacttc
    13981 agaccctaat cctgcagccc ccgacagcga gtcagtgatt gttgctatgg agcgggtatc
    14041 tgttcttttt gataggatca ggaaaggctt tccttgtgaa gccagagtgg tggccaggat
    14101 cctgccccag tttctagacg acttcttccc accccaggac atcatgaaca aagtcatcgg
    14161 agagtttctg tccaaccagc agccataccc ccagttcatg gccaccgtgg tgtataaggt
    14221 gtttcagact ctgcacagca ccgggcagtc gtccatggtc cgggactggg tcatgctgtc
    14281 cctctccaac ttcacgcaga gggccccggt cgccatggcc acgtggagcc tctcctgctt
    14341 ctttgtcagc gcgtccacca gcccgtgggt cgcggcgatc ctcccacatg tcatcagcag
    14401 gatgggcaag ctggagcagg tggacgtgaa ccttttctgc ctggtcgcca cagacttcta
    14461 cagacaccag atagaggagg agctcgaccg cagggccttc cagtctgtgc ttgaggtggt
    14521 tgcagcccca ggaagcccat atcaccggct gctgacttgt ttacgaaatg tccacaaggt
    14581 caccacctgc ggtggaagcg gtgactacaa agacgatgac gacaagtaaa acctctactt
    14641 ccaatcgcac catcatcacc atcaccatca ccaccatgat tacaaggatg acgacgataa
    14701 gtgaggcggc cgccaacttg atatcaagct tatcgataat caacctctgg attacaaaat
    14761 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
    14821 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
    14881 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
    14941 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
    15001 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
    15061 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
    15121 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct
    15181 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
    15241 cgggcgcccc cctcctcacg gcgagcgaag cttgtcgaga agtactagag gatctctaga
    15301 gcctgcagtc tcgacaagct tgtcgagaag tactagagga tcataatcag ccataccaca
    15361 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
    15421 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
    15481 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
    15541 ttgtccaaac tcatcaatgt atcttatcat gtctggatct gatcactgct tgagcctagg
    15601 agatccgaac cagataagtg aaatctagtt ccaaactatt ttgtcatttt taattttcgt
    15661 attagcttac gacgctacac ccagttccca tctattttgt cactcttccc taaataatcc
    15721 ttaaaaactc catttccacc cctcccagtt cccaactatt ttgtccgccc acagcggggc
    15781 atttttcttc ctgttatgtt tttaatcaaa catcctgcca actccatgtg acaaaccgtc
    15841 atcttcggct acttt
//
