LOCUS       sequence-226921-       16128 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus transfer vector for expression of huntingtin protein in 
            insect and mammalian cellscells as well as in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16128)
  AUTHORS   Harding RJ, Loppnau P, Ackloo S, Lemak A, Hutchinson A, Hunt B, 
            Holehouse AS, Ho JC, Fan L, Toledo-Sherman L, Seitova A, Arrowsmith 
            CH
  TITLE     Design and characterization of mutant and wildtype huntingtin 
            proteins produced from a toolkit of scalable eukaryotic expression 
            systems.
  JOURNAL   J Biol Chem. 2019 Apr 26;294(17):6986-7001. doi: 
            10.1074/jbc.RA118.007204. Epub 2019 Mar 6.
  PUBMED    30842263
REFERENCE   2  (bases 1 to 16128)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.007204"; journalName: "J Biol Chem"; date: 
            "2019-04-26- 26"; volume: "294"; issue: "17"; pages: "6986-7001"
COMMENT     Sequence Label: pBacMam2-DiEx-LIC-N-flag_huntingtin_full-lengt...
FEATURES             Location/Qualifiers
     source          1..16128
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      102..556
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(189..208)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     398..419
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        582..686
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(687..755,756..1547)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(905..924)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1718..2306
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2207..2226
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2460..2477
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  2611..2835
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2902..3435)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3624..3629,3630..3646,3647..3652))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     polyA_signal    4160..4208
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     4233..4252
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     enhancer        4313..4692
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4693..4896
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4847..4867
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        4943..5052
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     CDS             5087..5110
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      14217..14226
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             14921..14950
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             14951..14974
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(15064..15084)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(15482..15493)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    15718..15852
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(15755..15774)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     15809..15828
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  15881..16046
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga
       61 ctgaatatca acgcttattt gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
      121 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
      181 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
      241 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
      301 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
      361 ttttcgccct ttgacgttgg agtccacgtt cttaatagtg gactcttgtt ccaaactgga
      421 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
      481 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
      541 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
      601 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
      661 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
      721 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
      781 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
      841 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
      901 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
      961 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     1021 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     1081 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     1141 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     1201 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     1261 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     1321 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     1381 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     1441 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     1501 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     1561 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     1621 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     1681 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     1741 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     1801 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     1861 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     1921 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     1981 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     2041 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     2101 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     2161 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     2221 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     2281 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     2341 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     2401 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     2461 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc
     2521 tgtgcggtat ttcacaccgc atagaccagc cgcgtaacct ggcaaaatcg gttacggttg
     2581 agtaataaat ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa
     2641 caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga
     2701 aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact
     2761 cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg
     2821 gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc
     2881 gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac
     2941 ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc
     3001 ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttcaaga gattgatgag
     3061 cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat
     3121 ctcactacgc ggctgctcaa acttgggcag aacgtaagcc gcgagagcgc caacaaccgc
     3181 ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta
     3241 atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag
     3301 atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat
     3361 gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt
     3421 gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg
     3481 cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga
     3541 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg
     3601 agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc
     3661 gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg
     3721 aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg
     3781 cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg cccttgcttc
     3841 aggagatcgg tagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag
     3901 tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag gactctagct
     3961 atagttctag tggttggcta cgtacccgta gtggctatgg cagggcttgc cgccccgacg
     4021 ttggctgcga gccctgggcc ttcacccgaa cttgggggtt ggggtgggga aaaggaagaa
     4081 acgcgggcgt attggtccca atggggtctc ggtggggtat cgacagagtg ccagccctgg
     4141 gaccgaaccc cgcgtttatg aacaaacgac ccaacacccg tgcgttttat tctgtctttt
     4201 tattgccgtc atagcgcggg ttccttccgg tattgtctcc ttccgtgttt cagttagcct
     4261 cccccatctc ccggtaccgc atgcggatct gtacgggcca gatatacgcg ttgacattga
     4321 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     4381 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     4441 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     4501 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     4561 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     4621 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     4681 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     4741 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     4801 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     4861 aggcgtgtac ggtgggaggt ctatataagc agagctctct ggctaactag agaacccact
     4921 gcttactggc ttatcgaata cggaccttta attcaaccca acacaatata ttatagttaa
     4981 ataagaatta ttatcaaatc atttgtatat taattaaaat actatactgt aaattacatt
     5041 ttatttacaa tcactcgacg gatccttaag aaggagatat actatggact acaaagacga
     5101 tgacgacaag atggcgaccc tggaaaagct gatgaaggcc ttcgagtccc tcaagtcctt
     5161 ccaacagcag caacagcaac aacagcagca acagcaacaa cagcagcaac agcaacaaca
     5221 gcagcaacag caacaacagc agcaacagca acaacagcag caacagcaac aacagcagca
     5281 acagcaacaa cagcagcaac agcaacaaca gcagcaacag caacaacagc agcaacagca
     5341 acaacagcag caacagcaac aacagcagca acagcaacaa caacagcagc aacagcaaca
     5401 acagcagcaa cagcaacaac agcagcaaca gcaacaacag cagcaacagc aacaacagca
     5461 gcaacagcaa caacagcagc aacagcaaca acagcaacaa cagcagcaac agcaacaaca
     5521 gcagcaacag caacaacagc agcaacagca acaacagcag caacagcaac aacagcagca
     5581 acagcaaccg ccaccgccgc cgccgccgcc gccgcctcct cagcttcctc agccgccgcc
     5641 gcaggcacag ccgctgctgc ctcagccgca gccgcccccg ccgccgcccc cgccgccacc
     5701 cggcccggct gtggctgagg agccgctgca ccgaccaaag aaagaacttt cagctaccaa
     5761 gaaagaccgt gtgaatcatt gtctgacaat atgtgaaaac atagtggcac agtctgtcag
     5821 aaattctcca gaatttcaga aacttctggg catcgctatg gaactttttc tgctgtgcag
     5881 tgatgacgca gagtcagatg tcaggatggt ggctgacgaa tgcctcaaca aagttatcaa
     5941 agctttgatg gattctaatc ttccaaggtt acagctcgag ctctataagg aaattaaaaa
     6001 gaatggtgcc cctcggagtt tgcgtgctgc cctgtggagg tttgctgagc tggctcacct
     6061 ggttcggcct cagaaatgca ggccttacct ggtgaacctt ctgccgtgcc tgactcgaac
     6121 aagcaagaga cccgaagaat cagtccagga gaccttggct gcagctgttc ccaaaattat
     6181 ggcttctttt ggcaattttg caaatgacaa tgaaattaag gttttgttaa aggccttcat
     6241 agcgaacctg aagtcaagct cccccaccat tcggcggaca gcggctggat cagcagtgag
     6301 catctgccag cactcaagaa ggacacaata tttctatagt tggctactaa atgtgctctt
     6361 aggcttactc gttcctgtcg aggatgaaca ctccactctg ctgattcttg gcgtgctgct
     6421 caccctgagg tatttggtgc ccttgctgca gcagcaggtc aaggacacaa gcctgaaagg
     6481 cagcttcgga gtgacaagga aagaaatgga agtctctcct tctgcagagc agcttgtcca
     6541 ggtttatgaa ctgacgttac atcatacaca gcaccaagac cacaatgttg tgaccggagc
     6601 cctggagctg ttgcagcagc tcttcagaac gcctccaccc gagcttctgc aaaccctgac
     6661 cgcagtcggg ggcattgggc agctcaccgc tgctaaggag gagtctggtg gccgaagccg
     6721 tagtgggagt attgtggaac ttatagctgg agggggttcc tcatgcagcc ctgtcctttc
     6781 aagaaaacaa aaaggcaaag tgctcttagg agaagaagaa gccttggagg atgactctga
     6841 atcgagatcg gatgtcagca gctctgcctt aacagcctca gtgaaggatg agatcagtgg
     6901 agagctggct gcttcttcag gggtttccac tccagggtca gcaggtcatg acatcatcac
     6961 agaacagcca cggtcacagc acacactgca ggcggactca gtggatctgg ccagctgtga
     7021 cttgacaagc tctgccactg atggggatga ggaggatatc ttgagccaca gctccagcca
     7081 ggtcagcgcc gtcccatctg accctgccat ggacctgaat gatgggaccc aggcctcgtc
     7141 gcccatcagc gacagctccc agaccaccac cgaagggcct gattcagctg ttaccccttc
     7201 agacagttct gaaattgtgt tagacggtac cgacaaccag tatttgggcc tgcagattgg
     7261 acagccccag gatgaagatg aggaagccac aggtattctt cctgatgaag cctcggaggc
     7321 cttcaggaac tcttccatgg cccttcaaca ggcacattta ttgaaaaaca tgagtcactg
     7381 caggcagcct tctgacagca gtgttgataa atttgtgttg agagatgaag ctactgaacc
     7441 gggtgatcaa gaaaacaagc cttgccgcat caaaggtgac attggacagt ccactgatga
     7501 tgactctgca cctcttgtcc attgtgtccg ccttttatct gcttcgtttt tgctaacagg
     7561 gggaaaaaat gtgctggttc cggacaggga tgtgagggtc agcgtgaagg ccctggccct
     7621 cagctgtgtg ggagcagctg tggccctcca cccggaatct ttcttcagca aactctataa
     7681 agttcctctt gacaccacgg aataccctga ggaacagtat gtctcagaca tcttgaacta
     7741 catcgatcat ggagacccac aggttcgagg agccactgcc attctctgtg ggaccctcat
     7801 ctgctccatc ctcagcaggt cccgcttcca cgtgggagat tggatgggca ccattagaac
     7861 cctcacagga aatacatttt ctttggcgga ttgcattcct ttgctgcgga aaacactgaa
     7921 ggatgagtct tctgttactt gcaagttagc ttgtacagct gtgaggaact gtgtcatgag
     7981 tctctgcagc agcagctaca gtgagttagg actgcagctg atcatcgatg tgctgactct
     8041 gaggaacagt tcctattggc tggtgaggac agagcttctg gaaacccttg cagagattga
     8101 cttcaggctg gtgagctttt tggaggcaaa agcagaaaac ttacacagag gggctcatca
     8161 ttatacaggg cttttaaaac tgcaagaacg agtgctcaat aatgttgtca tccatttgct
     8221 tggagatgaa gaccccaggg tgcgacatgt tgccgcagca tcactaatta ggcttgtccc
     8281 aaagctgttt tataaatgtg accaaggaca agctgatcca gtagtggccg tggcaagaga
     8341 tcaaagcagt gtttacctga aacttctcat gcatgagacg cagcctccat ctcatttctc
     8401 cgtcagcaca ataaccagaa tatatagagg ctataaccta ctaccaagca taacagacgt
     8461 cactatggaa aataaccttt caagagttat tgcagcagtt tctcatgaac taatcacatc
     8521 aaccaccaga gcactcacat ttggatgctg tgaagctttg tgtcttcttt ccactgcctt
     8581 cccagtttgc atttggagtt taggttggca ctgtggagtg cctccactga gtgcctcaga
     8641 tgagtctagg aagagctgta ccgttgggat ggccacaatg attctgaccc tgctctcgtc
     8701 agcttggttc ccattggatc tctcagccca tcaagatgct ttgattttgg ccggaaactt
     8761 gcttgcagcc agtgctccca aatctctgag aagttcatgg gcctctgaag aagaagccaa
     8821 cccagcagcc accaagcaag aggaggtctg gccagccctg ggggaccggg ccctggtgcc
     8881 catggtggag cagctcttct ctcacctgct gaaggtgatt aacatttgtg cccacgtcct
     8941 ggatgacgtg gctcctggac ccgcaataaa ggcagccttg ccttctctaa caaacccccc
     9001 ttctctaagt cccatccgac gaaaggggaa ggagaaagaa ccaggagaac aagcatctgt
     9061 accgttgagt cccaagaaag gcagtgaggc cagtgcagct tctagacaat ctgatacctc
     9121 aggtcctgtt acaacaagta aatcctcatc actggggagt ttctatcatc ttccttcata
     9181 cctcaaactg catgatgtcc tgaaagctac acacgctaac tacaaggtca cgctggatct
     9241 tcagaacagc acggaaaagt ttggagggtt tcttcgctca gccttggatg ttctttctca
     9301 gatactagag ctggccacac tgcaggacat tgggaagtgt gttgaagaga tcctaggata
     9361 cctgaaatcc tgctttagtc gagaaccaat gatggcaact gtttgtgttc aacaattgtt
     9421 gaagactctc tttggcacaa acttggcctc ccagtttgat ggcttatctt ccaaccccag
     9481 caagtcacaa ggccgagcac agcgccttgg ctcctccagt gtgaggccag gcttgtacca
     9541 ctactgcttc atggccccgt acacccactt cacccaggcc ctcgctgacg ccagcctgag
     9601 gaacatggtg caggcggagc aggagaacga cacctcggga tggtttgatg tcctccagaa
     9661 agtgtctacc cagttgaaga caaacctcac gagtgtcaca aagaaccgtg cagataagaa
     9721 tgctattcat aatcacattc gtttgtttga acctcttgtt ataaaagctt taaaacagta
     9781 cacgactaca acatgtgtgc agttacagaa gcaggtttta gatttgctgg cgcagctggt
     9841 tcagttacgg gttaattact gtcttctgga ttcagatcag gtgtttattg gctttgtatt
     9901 gaaacagttt gaatacattg aagtgggcca gttcagggaa tcagaggcaa tcattccaaa
     9961 catctttttc ttcttggtat tactatctta tgaacgctat cattcaaaac agatcattgg
    10021 aattcctaaa atcattcagc tctgtgatgg catcatggcc agtggaagga aggctgtgac
    10081 acatgccata ccggctctgc agcccatagt ccacgacctc tttgtattaa gaggaacaaa
    10141 taaagctgat gcaggaaaag agcttgaaac ccaaaaagag gtggtggtgt caatgttact
    10201 gagactcatc cagtaccatc aggtgttgga gatgttcatt cttgtcctgc agcagtgcca
    10261 caaggagaat gaagacaagt ggaagcgact gtctcgacag atagctgaca tcatcctccc
    10321 aatgttagcc aaacagcaga tgcacattga ctctcatgaa gcccttggag tgttaaatac
    10381 attatttgag attttggccc cttcctccct ccgtccggta gacatgcttt tacggagtat
    10441 gttcgtcact ccaaacacaa tggcgtccgt gagcactgtt caactgtgga tatcgggaat
    10501 tctggccatt ttgagggttc tgatttccca gtcaactgaa gatattgttc tttctcgtat
    10561 tcaggagctc tccttctctc cgtatttaat ctcctgtaca gtaattaata ggttaagaga
    10621 tggggacagt acttcaacgc tagaagaaca cagtgaaggg aaacaaataa agaatttgcc
    10681 agaagaaaca ttttcaaggt ttctattaca actggttggt attcttttag aagacattgt
    10741 tacaaaacag ctgaaggtgg aaatgagtga gcagcaacat actttctatt gccaggaact
    10801 aggcacactg ctaatgtgtc tgatccacat cttcaagtct ggaatgttcc ggagaatcac
    10861 agcagctgcc actaggctgt tccgcagtga tggctgtggc ggcagtttct acaccctgga
    10921 cagcttgaac ttgcgggctc gttccatgat caccacccac ccggccctgg tgctgctctg
    10981 gtgtcagata ctgctgcttg tcaaccacac cgactaccgc tggtgggcag aagtgcagca
    11041 gaccccgaaa agacacagtc tgtccagcac aaagttactt agtccccaga tgtctggaga
    11101 agaggaggat tctgacttgg cagccaaact tggaatgtgc aatagagaaa tagtacgaag
    11161 aggggctctc attctcttct gtgattatgt ctgtcagaac ctccatgact ccgagcactt
    11221 aacgtggctc attgtaaatc acattcaaga tctgatcagc ctttcccacg agcctccagt
    11281 acaggacttc atcagtgccg ttcatcggaa ctctgctgcc agcggcctgt tcatccaggc
    11341 aattcagtct cgttgtgaaa acctttcaac tccaaccatg ctgaagaaaa ctcttcagtg
    11401 cttggagggg atccatctca gccagtcggg agctgtgctc acgctgtatg tggacaggct
    11461 tctgtgcacc cctttccgtg tgctggctcg catggtcgac atccttgctt gtcgccgggt
    11521 agaaatgctt ctggctgcaa atttacagag cagcatggcc cagttgccaa tggaagaact
    11581 caacagaatc caggaatacc ttcagagcag cgggctcgct cagagacacc aaaggctcta
    11641 ttccctgctg gacaggtttc gtctctccac catgcaagac tcacttagtc cctctcctcc
    11701 agtctcttcc cacccgctgg acggggatgg gcacgtgtca ctggaaacag tgagtccgga
    11761 caaagactgg tacgttcatc ttgtcaaatc ccagtgttgg accaggtcag attctgcact
    11821 gctggaaggt gcagagctgg tgaatcggat tcctgctgaa gatatgaatg ccttcatgat
    11881 gaactcggag ttcaacctaa gcctgctagc tccatgctta agcctaggga tgagtgaaat
    11941 ttctggtggc cagaagagtg ccctttttga agcagcccgt gaggtgactc tggcccgtgt
    12001 gagcggcacc gtgcagcagc tccctgctgt ccatcatgtc ttccagcccg agctgcctgc
    12061 agaaccggcg gcctactgga gcaagttgaa tgatctgttt ggggatgctg cactgtatca
    12121 gtccctgccc actctggccc gggccctggc acagtacctg gtggtggtct ccaaactgcc
    12181 cagtcatttg caccttcctc ctgagaaaga gaaggacatt gtgaaattcg tggtggcaac
    12241 ccttgaggcc ctgtcctggc atttgatcca tgagcagatc ccgctgagtc tggatctcca
    12301 ggcagggctg gactgctgct gcctggccct gcagctgcct ggcctctgga gcgtggtctc
    12361 ctccacagag tttgtgaccc acgcctgctc cctcatccac tgtgtgcact tcatcctgga
    12421 ggccgttgca gtgcagcctg gagagcagct tcttagtcca gaaagaagga caaatacccc
    12481 aaaagccatc agcgaggagg aggaggaagt agatccaaac acacagaatc ctaagtatat
    12541 cactgcagcc tgtgagatgg tggcagaaat ggtggagtct ctgcagtcgg tgttggcctt
    12601 gggtcataaa aggaatagcg gcgtgccggc gtttctcacg ccattgctca ggaacatcat
    12661 catcagcctg gcccgcctgc cccttgtcaa cagctacaca cgtgtgcccc cactggtgtg
    12721 gaagcttgga tggtcaccca aaccgggagg ggattttggc acagcattcc ctgagatccc
    12781 cgtggagttc ctccaggaaa aggaagtctt taaggagttc atctaccgca tcaacacact
    12841 aggctggacc agtcgtactc agtttgaaga aacttgggcc accctccttg gtgtcctggt
    12901 gacgcagccc ctcgtgatgg agcaggagga gagcccacca gaagaagaca cagagaggac
    12961 ccagatcaac gtcctggccg tgcaggccat cacctcactg gtgctcagtg caatgactgt
    13021 gcctgtggcc ggcaacccag ctgtaagctg cttggagcag cagccccgga acaagcctct
    13081 gaaagctctc gacaccaggt ttgggaggaa gctgagcatt atcagaggga ttgtggagca
    13141 agagattcaa gcaatggttt caaagagaga gaatattgcc acccatcatt tatatcaggc
    13201 atgggatcct gtcccttctc tgtctccggc tactacaggt gccctcatca gccacgagaa
    13261 gctgctgctg cagatcaacc ccgagcggga gctggggagc atgagctaca aactcggcca
    13321 ggtgtccata cactccgtgt ggctagggaa cagcatcaca cccctgaggg aggaggaatg
    13381 ggacgaggaa gaggaggagg aggccgacgc ccctgcacct tcgtcaccac ccacgtctcc
    13441 agtcaactcc aggaaacacc gggctggagt tgacatccac tcctgttcgc agtttttgct
    13501 tgagttgtac agccgctgga tcctgccgtc cagctcagcc aggaggaccc cggccatcct
    13561 gatcagtgag gtggtcagat cccttctagt ggtctcagac ttgttcaccg agcgcaacca
    13621 gtttgagctg atgtatgtga cgctgacaga actgcgaagg gtgcaccctt cagaagacga
    13681 gatcctcgct cagtacctgg tgcctgccac ctgcaaggca gctgccgtcc ttgggatgga
    13741 caaggccgtg gcggagcctg tcagccgcct gctggagagc acgctcagga gcagccacct
    13801 gcccagcagg gttggagccc tgcacggcat cctctatgtg ctggagtgcg acctgctgga
    13861 cgacactgcc aagcagctca tcccggtcat cagcgactat ctcctctcca acctgaaagg
    13921 gatcgcccac tgcgtgaaca ttcacagcca gcagcacgta ctggtcatgt gtgccactgc
    13981 gttttacctc attgagaact atcctctgga cgtagggccg gaattttcag catcaataat
    14041 acagatgtgt ggggtgatgc tgtctggaag tgaggagtcc accccctcca tcatttacca
    14101 ctgtgccctc agaggcctgg agcgcctcct gctctctgag cagctctccc gcctggatgc
    14161 agaatcgctg gtcaagctga gtgtggacag agtgaacgtg cacagcccgc accgggccat
    14221 ggcggctctg ggcctgatgc tcacctgcat gtacacagga aaggagaaag tcagtccggg
    14281 tagaacttca gaccctaatc ctgcagcccc cgacagcgag tcagtgattg ttgctatgga
    14341 gcgggtatct gttctttttg ataggatcag gaaaggcttt ccttgtgaag ccagagtggt
    14401 ggccaggatc ctgccccagt ttctagacga cttcttccca ccccaggaca tcatgaacaa
    14461 agtcatcgga gagtttctgt ccaaccagca gccatacccc cagttcatgg ccaccgtggt
    14521 gtataaggtg tttcagactc tgcacagcac cgggcagtcg tccatggtcc gggactgggt
    14581 catgctgtcc ctctccaact tcacgcagag ggccccggtc gccatggcca cgtggagcct
    14641 ctcctgcttc tttgtcagcg cgtccaccag cccgtgggtc gcggcgatcc tcccacatgt
    14701 catcagcagg atgggcaagc tggagcaggt ggacgtgaac cttttctgcc tggtcgccac
    14761 agacttctac agacaccaga tagaggagga gctcgaccgc agggccttcc agtctgtgct
    14821 tgaggtggtt gcagccccag gaagcccata tcaccggctg ctgacttgtt tacgaaatgt
    14881 ccacaaggtc accacctgct aaaacctcta cttccaatcg caccatcatc accatcacca
    14941 tcaccaccat gattacaagg atgacgacga taagtgaggc ggccgccaac ttgatatcaa
    15001 gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
    15061 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
    15121 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
    15181 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
    15241 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
    15301 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
    15361 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
    15421 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
    15481 ttcggccctc aatccagcgg accttccttc ccgcgggcgc ccccctcctc acggcgagcg
    15541 aagcttgtcg agaagtacta gaggatctct agagcctgca gtctcgacaa gcttgtcgag
    15601 aagtactaga ggatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
    15661 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
    15721 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
    15781 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
    15841 catgtctgga tctgatcact gcttgagcct aggagatccg aaccagataa gtgaaatcta
    15901 gttccaaact attttgtcat ttttaatttt cgtattagct tacgacgcta cacccagttc
    15961 ccatctattt tgtcactctt ccctaaataa tccttaaaaa ctccatttcc acccctccca
    16021 gttcccaact attttgtccg cccacagcgg ggcatttttc ttcctgttat gtttttaatc
    16081 aaacatcctg ccaactccat gtgacaaacc gtcatcttcg gctacttt
//
