LOCUS       sequence-226923-       15974 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus transfer vector for expression of huntingtin protein in 
            insect and mammalian cellscells as well as in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15974)
  AUTHORS   Harding RJ, Loppnau P, Ackloo S, Lemak A, Hutchinson A, Hunt B, 
            Holehouse AS, Ho JC, Fan L, Toledo-Sherman L, Seitova A, Arrowsmith 
            CH
  TITLE     Design and characterization of mutant and wildtype huntingtin 
            proteins produced from a toolkit of scalable eukaryotic expression 
            systems.
  JOURNAL   J Biol Chem. 2019 Apr 26;294(17):6986-7001. doi: 
            10.1074/jbc.RA118.007204. Epub 2019 Mar 6.
  PUBMED    30842263
REFERENCE   2  (bases 1 to 15974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.007204"; journalName: "J Biol Chem"; date: 
            "2019-04-26- 26"; volume: "294"; issue: "17"; pages: "6986-7001"
COMMENT     Sequence Label: pBacMam2-DiEx-LIC-N-flag_huntingtin_full-lengt...
FEATURES             Location/Qualifiers
     source          1..15974
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      102..556
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(189..208)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     398..419
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        582..686
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(687..755,756..1547)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(905..924)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1718..2306
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2207..2226
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2460..2477
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  2611..2835
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2902..3435)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3624..3629,3630..3646,3647..3652))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     polyA_signal    4160..4208
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     4233..4252
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     enhancer        4313..4692
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4693..4896
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4847..4867
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        4943..5052
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     CDS             5087..5110
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      14025..14034
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             14767..14796
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             14797..14820
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(14910..14930)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(15328..15339)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    15564..15698
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(15601..15620)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     15655..15674
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  15727..15892
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga
       61 ctgaatatca acgcttattt gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
      121 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
      181 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
      241 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
      301 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
      361 ttttcgccct ttgacgttgg agtccacgtt cttaatagtg gactcttgtt ccaaactgga
      421 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
      481 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
      541 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
      601 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
      661 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
      721 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
      781 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
      841 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
      901 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
      961 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     1021 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     1081 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     1141 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     1201 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     1261 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     1321 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     1381 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     1441 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     1501 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     1561 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     1621 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     1681 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     1741 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     1801 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     1861 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     1921 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     1981 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     2041 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     2101 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     2161 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     2221 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     2281 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     2341 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     2401 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     2461 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc
     2521 tgtgcggtat ttcacaccgc atagaccagc cgcgtaacct ggcaaaatcg gttacggttg
     2581 agtaataaat ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa
     2641 caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga
     2701 aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact
     2761 cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg
     2821 gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc
     2881 gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac
     2941 ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc
     3001 ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttcaaga gattgatgag
     3061 cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat
     3121 ctcactacgc ggctgctcaa acttgggcag aacgtaagcc gcgagagcgc caacaaccgc
     3181 ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta
     3241 atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag
     3301 atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat
     3361 gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt
     3421 gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg
     3481 cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga
     3541 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg
     3601 agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc
     3661 gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg
     3721 aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg
     3781 cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg cccttgcttc
     3841 aggagatcgg tagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag
     3901 tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag gactctagct
     3961 atagttctag tggttggcta cgtacccgta gtggctatgg cagggcttgc cgccccgacg
     4021 ttggctgcga gccctgggcc ttcacccgaa cttgggggtt ggggtgggga aaaggaagaa
     4081 acgcgggcgt attggtccca atggggtctc ggtggggtat cgacagagtg ccagccctgg
     4141 gaccgaaccc cgcgtttatg aacaaacgac ccaacacccg tgcgttttat tctgtctttt
     4201 tattgccgtc atagcgcggg ttccttccgg tattgtctcc ttccgtgttt cagttagcct
     4261 cccccatctc ccggtaccgc atgcggatct gtacgggcca gatatacgcg ttgacattga
     4321 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     4381 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     4441 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     4501 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     4561 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     4621 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     4681 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     4741 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     4801 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     4861 aggcgtgtac ggtgggaggt ctatataagc agagctctct ggctaactag agaacccact
     4921 gcttactggc ttatcgaata cggaccttta attcaaccca acacaatata ttatagttaa
     4981 ataagaatta ttatcaaatc atttgtatat taattaaaat actatactgt aaattacatt
     5041 ttatttacaa tcactcgacg gatccttaag aaggagatat actatggact acaaagacga
     5101 tgacgacaag atggcgaccc tggaaaagct gatgaaggcc ttcgagtccc tcaagtcctt
     5161 ccaacagcag caacagcaac aacagcagca acagcaacaa cagcagcaac agcaacaaca
     5221 gcagcaacag caacaacagc agcaacagca acaacagcag caacagcaac aacagcagca
     5281 acagcaacaa cagcagcaac agcaacaaca gcagcaacag caacaacagc agcaacagca
     5341 acaacagcag caacagcaac aacagcagca acagcaacaa cagcagcaac agcaaccgcc
     5401 accgccgccg ccgccgccgc cgcctcctca gcttcctcag ccgccgccgc aggcacagcc
     5461 cctgctgcct cagccgcagc cgcccccgcc gccgcccccg ccgccacccg gcccggctgt
     5521 ggctgaggag ccgctgcacc gaccaaagaa agaactttca gctaccaaga aagaccgtgt
     5581 gaatcattgt ctgacaatat gtgaaaacat agtggcacag tctgtcagaa attctccaga
     5641 atttcagaaa cttctgggca tcgctatgga actttttctg ctgtgcagtg atgacgcaga
     5701 gtcagatgtc aggatggtgg ctgacgaatg cctcaacaaa gttatcaaag ctttgatgga
     5761 ttctaatctt ccaaggttac agctcgagct ctataaggaa attaaaaaga atggtgcccc
     5821 tcggagtttg cgtgctgccc tgtggaggtt tgctgagctg gctcacctgg ttcggcctca
     5881 gaaatgcagg ccttacctgg tgaaccttct gccgtgcctg actcgaacaa gcaagagacc
     5941 cgaagaatca gtccaggaga ccttggctgc agctgttccc aaaattatgg cttcttttgg
     6001 caattttgca aatgacaatg aaattaaggt tttgttaaag gccttcatag cgaacctgaa
     6061 gtcaagctcc cccaccattc ggcggacagc ggctggatca gcagtgagca tctgccagca
     6121 ctcaagaagg acacaatatt tctatagttg gctactaaat gtgctcttag gcttactcgt
     6181 tcctgtcgag gatgaacact ccactctgct gattcttggc gtgctgctca ccctgaggta
     6241 tttggtgccc ttgctgcagc agcaggtcaa ggacacaagc ctgaaaggca gcttcggagt
     6301 gacaaggaaa gaaatggaag tctctccttc tgcagagcag cttgtccagg tttatgaact
     6361 gacgttacat catacacagc accaagacca caatgttgtg accggagccc tggagctgtt
     6421 gcagcagctc ttcagaacgc ctccacccga gcttctgcaa accctgaccg cagtcggggg
     6481 cattgggcag ctcaccgctg ctaaggagga gtctggtggc cgaagccgta gtgggagtat
     6541 tgtggaactt atagctggag ggggttcctc atgcagccct gtcctttcaa gaaaacaaaa
     6601 aggcaaagtg ctcttaggag aagaagaagc cttggaggat gactctgaat cgagatcgga
     6661 tgtcagcagc tctgccttaa cagcctcagt gaaggatgag atcagtggag agctggctgc
     6721 ttcttcaggg gtttccactc cagggtcagc aggtcatgac atcatcacag aacagccacg
     6781 gtcacagcac acactgcagg cggactcagt ggatctggcc agctgtgact tgacaagctc
     6841 tgccactgat ggggatgagg aggatatctt gagccacagc tccagccagg tcagcgccgt
     6901 cccatctgac cctgccatgg acctgaatga tgggacccag gcctcgtcgc ccatcagcga
     6961 cagctcccag accaccaccg aagggcctga ttcagctgtt accccttcag acagttctga
     7021 aattgtgtta gacggtaccg acaaccagta tttgggcctg cagattggac agccccagga
     7081 tgaagatgag gaagccacag gtattcttcc tgatgaagcc tcggaggcct tcaggaactc
     7141 ttccatggcc cttcaacagg cacatttatt gaaaaacatg agtcactgca ggcagccttc
     7201 tgacagcagt gttgataaat ttgtgttgag agatgaagct actgaaccgg gtgatcaaga
     7261 aaacaagcct tgccgcatca aaggtgacat tggacagtcc actgatgatg actctgcacc
     7321 tcttgtccat tgtgtccgcc ttttatctgc ttcgtttttg ctaacagggg gaaaaaatgt
     7381 gctggttccg gacagggatg tgagggtcag cgtgaaggcc ctggccctca gctgtgtggg
     7441 agcagctgtg gccctccacc cggaatcttt cttcagcaaa ctctataaag ttcctcttga
     7501 caccacggaa taccctgagg aacagtatgt ctcagacatc ttgaactaca tcgatcatgg
     7561 agacccacag gttcgaggag ccactgccat tctctgtggg accctcatct gctccatcct
     7621 cagcaggtcc cgcttccacg tgggagattg gatgggcacc attagaaccc tcacaggaaa
     7681 tacattttct ttggcggatt gcattccttt gctgcggaaa acactgaagg atgagtcttc
     7741 tgttacttgc aagttagctt gtacagctgt gaggaactgt gtcatgagtc tctgcagcag
     7801 cagctacagt gagttaggac tgcagctgat catcgatgtg ctgactctga ggaacagttc
     7861 ctattggctg gtgaggacag agcttctgga aacccttgca gagattgact tcaggctggt
     7921 gagctttttg gaggcaaaag cagaaaactt acacagaggg gctcatcatt atacagggct
     7981 tttaaaactg caagaacgag tgctcaataa tgttgtcatc catttgcttg gagatgaaga
     8041 ccccagggtg cgacatgttg ccgcagcatc actaattagg cttgtcccaa agctgtttta
     8101 taaatgtgac caaggacaag ctgatccagt agtggccgtg gcaagagatc aaagcagtgt
     8161 ttacctgaaa cttctcatgc atgagacgca gcctccatct catttctccg tcagcacaat
     8221 aaccagaata tatagaggct ataacctact accaagcata acagacgtca ctatggaaaa
     8281 taacctttca agagttattg cagcagtttc tcatgaacta atcacatcaa ccaccagagc
     8341 actcacattt ggatgctgtg aagctttgtg tcttctttcc actgccttcc cagtttgcat
     8401 ttggagttta ggttggcact gtggagtgcc tccactgagt gcctcagatg agtctaggaa
     8461 gagctgtacc gttgggatgg ccacaatgat tctgaccctg ctctcgtcag cttggttccc
     8521 attggatctc tcagcccatc aagatgcttt gattttggcc ggaaacttgc ttgcagccag
     8581 tgctcccaaa tctctgagaa gttcatgggc ctctgaagaa gaagccaacc cagcagccac
     8641 caagcaagag gaggtctggc cagccctggg ggaccgggcc ctggtgccca tggtggagca
     8701 gctcttctct cacctgctga aggtgattaa catttgtgcc cacgtcctgg atgacgtggc
     8761 tcctggaccc gcaataaagg cagccttgcc ttctctaaca aacccccctt ctctaagtcc
     8821 catccgacga aaggggaagg agaaagaacc aggagaacaa gcatctgtac cgttgagtcc
     8881 caagaaaggc agtgaggcca gtgcagcttc tagacaatct gatacctcag gtcctgttac
     8941 aacaagtaaa tcctcatcac tggggagttt ctatcatctt ccttcatacc tcaaactgca
     9001 tgatgtcctg aaagctacac acgctaacta caaggtcacg ctggatcttc agaacagcac
     9061 ggaaaagttt ggagggtttc ttcgctcagc cttggatgtt ctttctcaga tactagagct
     9121 ggccacactg caggacattg ggaagtgtgt tgaagagatc ctaggatacc tgaaatcctg
     9181 ctttagtcga gaaccaatga tggcaactgt ttgtgttcaa caattgttga agactctctt
     9241 tggcacaaac ttggcctccc agtttgatgg cttatcttcc aaccccagca agtcacaagg
     9301 ccgagcacag cgccttggct cctccagtgt gaggccaggc ttgtaccact actgcttcat
     9361 ggccccgtac acccacttca cccaggccct cgctgacgcc agcctgagga acatggtgca
     9421 ggcggagcag gagaacgaca cctcgggatg gtttgatgtc ctccagaaag tgtctaccca
     9481 gttgaagaca aacctcacga gtgtcacaaa gaaccgtgca gataagaatg ctattcataa
     9541 tcacattcgt ttgtttgaac ctcttgttat aaaagcttta aaacagtaca cgactacaac
     9601 atgtgtgcag ttacagaagc aggttttaga tttgctggcg cagctggttc agttacgggt
     9661 taattactgt cttctggatt cagatcaggt gtttattggc tttgtattga aacagtttga
     9721 atacattgaa gtgggccagt tcagggaatc agaggcaatc attccaaaca tctttttctt
     9781 cttggtatta ctatcttatg aacgctatca ttcaaaacag atcattggaa ttcctaaaat
     9841 cattcagctc tgtgatggca tcatggccag tggaaggaag gctgtgacac atgccatacc
     9901 ggctctgcag cccatagtcc acgacctctt tgtattaaga ggaacaaata aagctgatgc
     9961 aggaaaagag cttgaaaccc aaaaagaggt ggtggtgtca atgttactga gactcatcca
    10021 gtaccatcag gtgttggaga tgttcattct tgtcctgcag cagtgccaca aggagaatga
    10081 agacaagtgg aagcgactgt ctcgacagat agctgacatc atcctcccaa tgttagccaa
    10141 acagcagatg cacattgact ctcatgaagc ccttggagtg ttaaatacat tatttgagat
    10201 tttggcccct tcctccctcc gtccggtaga catgctttta cggagtatgt tcgtcactcc
    10261 aaacacaatg gcgtccgtga gcactgttca actgtggata tcgggaattc tggccatttt
    10321 gagggttctg atttcccagt caactgaaga tattgttctt tctcgtattc aggagctctc
    10381 cttctctccg tatttaatct cctgtacagt aattaatagg ttaagagatg gggacagtac
    10441 ttcaacgcta gaagaacaca gtgaagggaa acaaataaag aatttgccag aagaaacatt
    10501 ttcaaggttt ctattacaac tggttggtat tcttttagaa gacattgtta caaaacagct
    10561 gaaggtggaa atgagtgagc agcaacatac tttctattgc caggaactag gcacactgct
    10621 aatgtgtctg atccacatct tcaagtctgg aatgttccgg agaatcacag cagctgccac
    10681 taggctgttc cgcagtgatg gctgtggcgg cagtttctac accctggaca gcttgaactt
    10741 gcgggctcgt tccatgatca ccacccaccc ggccctggtg ctgctctggt gtcagatact
    10801 gctgcttgtc aaccacaccg actaccgctg gtgggcagaa gtgcagcaga ccccgaaaag
    10861 acacagtctg tccagcacaa agttacttag tccccagatg tctggagaag aggaggattc
    10921 tgacttggca gccaaacttg gaatgtgcaa tagagaaata gtacgaagag gggctctcat
    10981 tctcttctgt gattatgtct gtcagaacct ccatgactcc gagcacttaa cgtggctcat
    11041 tgtaaatcac attcaagatc tgatcagcct ttcccacgag cctccagtac aggacttcat
    11101 cagtgccgtt catcggaact ctgctgccag cggcctgttc atccaggcaa ttcagtctcg
    11161 ttgtgaaaac ctttcaactc caaccatgct gaagaaaact cttcagtgct tggaggggat
    11221 ccatctcagc cagtcgggag ctgtgctcac gctgtatgtg gacaggcttc tgtgcacccc
    11281 tttccgtgtg ctggctcgca tggtcgacat ccttgcttgt cgccgggtag aaatgcttct
    11341 ggctgcaaat ttacagagca gcatggccca gttgccaatg gaagaactca acagaatcca
    11401 ggaatacctt cagagcagcg ggctcgctca gagacaccaa aggctctatt ccctgctgga
    11461 caggtttcgt ctctccacca tgcaagactc acttagtccc tctcctccag tctcttccca
    11521 cccgctggac ggggatgggc acgtgtcact ggaaacagtg agtccggaca aagactggta
    11581 cgttcatctt gtcaaatccc agtgttggac caggtcagat tctgcactgc tggaaggtgc
    11641 agagctggtg aatcggattc ctgctgaaga tatgaatgcc ttcatgatga actcggagtt
    11701 caacctaagc ctgctagctc catgcttaag cctagggatg agtgaaattt ctggtggcca
    11761 gaagagtgcc ctttttgaag cagcccgtga ggtgactctg gcccgtgtga gcggcaccgt
    11821 gcagcagctc cctgctgtcc atcatgtctt ccagcccgag ctgcctgcag aaccggcggc
    11881 ctactggagc aagttgaatg atctgtttgg ggatgctgca ctgtatcagt ccctgcccac
    11941 tctggcccgg gccctggcac agtacctggt ggtggtctcc aaactgccca gtcatttgca
    12001 ccttcctcct gagaaagaga aggacattgt gaaattcgtg gtggcaaccc ttgaggccct
    12061 gtcctggcat ttgatccatg agcagatccc gctgagtctg gatctccagg cagggctgga
    12121 ctgctgctgc ctggccctgc agctgcctgg cctctggagc gtggtctcct ccacagagtt
    12181 tgtgacccac gcctgctccc tcatccactg tgtgcacttc atcctggagg ccgttgcagt
    12241 gcagcctgga gagcagcttc ttagtccaga aagaaggaca aataccccaa aagccatcag
    12301 cgaggaggag gaggaagtag atccaaacac acagaatcct aagtatatca ctgcagcctg
    12361 tgagatggtg gcagaaatgg tggagtctct gcagtcggtg ttggccttgg gtcataaaag
    12421 gaatagcggc gtgccggcgt ttctcacgcc attgctcagg aacatcatca tcagcctggc
    12481 ccgcctgccc cttgtcaaca gctacacacg tgtgccccca ctggtgtgga agcttggatg
    12541 gtcacccaaa ccgggagggg attttggcac agcattccct gagatccccg tggagttcct
    12601 ccaggaaaag gaagtcttta aggagttcat ctaccgcatc aacacactag gctggaccag
    12661 tcgtactcag tttgaagaaa cttgggccac cctccttggt gtcctggtga cgcagcccct
    12721 cgtgatggag caggaggaga gcccaccaga agaagacaca gagaggaccc agatcaacgt
    12781 cctggccgtg caggccatca cctcactggt gctcagtgca atgactgtgc ctgtggccgg
    12841 caacccagct gtaagctgct tggagcagca gccccggaac aagcctctga aagctctcga
    12901 caccaggttt gggaggaagc tgagcattat cagagggatt gtggagcaag agattcaagc
    12961 aatggtttca aagagagaga atattgccac ccatcattta tatcaggcat gggatcctgt
    13021 cccttctctg tctccggcta ctacaggtgc cctcatcagc cacgagaagc tgctgctgca
    13081 gatcaacccc gagcgggagc tggggagcat gagctacaaa ctcggccagg tgtccataca
    13141 ctccgtgtgg ctagggaaca gcatcacacc cctgagggag gaggaatggg acgaggaaga
    13201 ggaggaggag gccgacgccc ctgcaccttc gtcaccaccc acgtctccag tcaactccag
    13261 gaaacaccgg gctggagttg acatccactc ctgttcgcag tttttgcttg agttgtacag
    13321 ccgctggatc ctgccgtcca gctcagccag gaggaccccg gccatcctga tcagtgaggt
    13381 ggtcagatcc cttctagtgg tctcagactt gttcaccgag cgcaaccagt ttgagctgat
    13441 gtatgtgacg ctgacagaac tgcgaagggt gcacccttca gaagacgaga tcctcgctca
    13501 gtacctggtg cctgccacct gcaaggcagc tgccgtcctt gggatggaca aggccgtggc
    13561 ggagcctgtc agccgcctgc tggagagcac gctcaggagc agccacctgc ccagcagggt
    13621 tggagccctg cacggcatcc tctatgtgct ggagtgcgac ctgctggacg acactgccaa
    13681 gcagctcatc ccggtcatca gcgactatct cctctccaac ctgaaaggga tcgcccactg
    13741 cgtgaacatt cacagccagc agcacgtact ggtcatgtgt gccactgcgt tttacctcat
    13801 tgagaactat cctctggacg tagggccgga attttcagca tcaataatac agatgtgtgg
    13861 ggtgatgctg tctggaagtg aggagtccac cccctccatc atttaccact gtgccctcag
    13921 aggcctggag cgcctcctgc tctctgagca gctctcccgc ctggatgcag aatcgctggt
    13981 caagctgagt gtggacagag tgaacgtgca cagcccgcac cgggccatgg cggctctggg
    14041 cctgatgctc acctgcatgt acacaggaaa ggagaaagtc agtccgggta gaacttcaga
    14101 ccctaatcct gcagcccccg acagcgagtc agtgattgtt gctatggagc gggtatctgt
    14161 tctttttgat aggatcagga aaggctttcc ttgtgaagcc agagtggtgg ccaggatcct
    14221 gccccagttt ctagacgact tcttcccacc ccaggacatc atgaacaaag tcatcggaga
    14281 gtttctgtcc aaccagcagc cataccccca gttcatggcc accgtggtgt ataaggtgtt
    14341 tcagactctg cacagcaccg ggcagtcgtc catggtccgg gactgggtca tgctgtccct
    14401 ctccaacttc acgcagaggg ccccggtcgc catggccacg tggagcctct cctgcttctt
    14461 tgtcagcgcg tccaccagcc cgtgggtcgc ggcgatcctc ccacatgtca tcagcaggat
    14521 gggcaagctg gagcaggtgg acgtgaacct tttctgcctg gtcgccacag acttctacag
    14581 acaccagata gaggaggagc tcgaccgcag ggccttccag tctgtgcttg aggtggttgc
    14641 agccccagga agcccatatc accggctgct gacttgktwr csmmakgwms mcmakrtcac
    14701 crsctgctga cttgtttacg aaatgtccac aaggtcacca cctgctaaaa cctctacttc
    14761 caatcgcacc atcatcacca tcaccatcac caccatgatt acaaggatga cgacgataag
    14821 tgaggcggcc gccaacttga tatcaagctt atcgataatc aacctctgga ttacaaaatt
    14881 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
    14941 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
    15001 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
    15061 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
    15121 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
    15181 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
    15241 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
    15301 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
    15361 gggcgccccc ctcctcacgg cgagcgaagc ttgtcgagaa gtactagagg atctctagag
    15421 cctgcagtct cgacaagctt gtcgagaagt actagaggat cataatcagc cataccacat
    15481 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
    15541 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
    15601 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
    15661 tgtccaaact catcaatgta tcttatcatg tctggatctg atcactgctt gagcctagga
    15721 gatccgaacc agataagtga aatctagttc caaactattt tgtcattttt aattttcgta
    15781 ttagcttacg acgctacacc cagttcccat ctattttgtc actcttccct aaataatcct
    15841 taaaaactcc atttccaccc ctcccagttc ccaactattt tgtccgccca cagcggggca
    15901 tttttcttcc tgttatgttt ttaatcaaac atcctgccaa ctccatgtga caaaccgtca
    15961 tcttcggcta cttt
//
