LOCUS       sequence-226932-       12106 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Sox10-MCS5-GFP and a constitutive EF1-alpha-mCherry reporter 
            plasmid. Construct generates constitutive red fluorescence and basal
            promoter cfos conjugated SOXMCS5 enhancer driven GFP expression in a
            cell..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12106)
  AUTHORS   Pol SU, Lang JK, O'Bara MA, Cimato TR, McCallion AS, Sim FJ
  TITLE     Sox10-MCS5 enhancer dynamically tracks human oligodendrocyte 
            progenitor fate.
  JOURNAL   Exp Neurol. 2013 Sep;247:694-702. doi: 
            10.1016/j.expneurol.2013.03.010. Epub 2013 Mar 16.
  PUBMED    23507034
REFERENCE   2  (bases 1 to 12106)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Exp 
            Neurol."; date: "2013-09"; volume: "247:694-702. doi"; pages: " 
            10.1016/j.expneurol.2013.03.010. Epub 2013 Mar 16"
COMMENT     Sequence Label: LV-Sox10MCS5-GFP-EF1a-mCherry
FEATURES             Location/Qualifiers
     source          1..12106
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             85..718
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    765..890
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1383..1616
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1801..1845
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2138..2255
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    complement(2861..2885)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(3050..3061)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     regulatory      3122..3131
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3128..3130,3131..3133,3134..3847)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFRIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3173..3194)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3434..3453)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3781..3802
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        3878..4089
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             4105..4815
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4250..4268)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4518..4537
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    4841..5429
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4894..4914)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5312..5323)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5610..5790
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7318..7337)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7551..7570)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7670..7692
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7730..7748)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7816..7920
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7921..7989,7990..8781)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8139..8158)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8952..9540
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9441..9460
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9694..9711
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9781..9801)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9786..10115
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9966..10101
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          11249..11314
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11444..11464
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11889..12023
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11926..11945)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11980..11999
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta
       61 aatatgtact acaaacttag tagttggaag ggctaattca ctcccaaaga agacaagata
      121 tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag aactacacac
      181 cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
      241 agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga
      301 gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc
      361 tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc
      421 gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga
      481 ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg
      541 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      601 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      661 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      721 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      781 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      841 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      901 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
      961 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1021 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1081 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1141 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1201 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1261 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1321 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1381 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1441 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1501 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1561 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1621 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1681 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1741 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1801 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1861 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1921 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     1981 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2041 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
     2101 tatcgccgaa ttcacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
     2161 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     2221 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
     2281 atccactttg gctgatacgc gtactagtct gggtccctcc ccacccagtc gccacccacg
     2341 cacactttcc ctctcgcctg gggcagctgg tgggaggggg ctgggcccgg ctgggcaact
     2401 gggggaggct gggaaagtcc aggctgtccc tcttgggggc ggggggtggg acacgggggc
     2461 tgtgtgctgg gatgctcact gggatggagg gagggagggt cgacagcccc tacagctgcg
     2521 gtccccttcc cagcagcctg cccaggcaca gaaaggtctc tttgttctct gcctttaaag
     2581 ggtaagagaa acacttctgc atggcaaatt agcccaaaaa tgtatttctc taaagcaccc
     2641 gattgtctcc ccagtctgca gctgcaggga agtcccaggg agctggggag gggcggcttt
     2701 ggggtgaccc tgggggacag ggagaggagg tggggaaaga ggggccccat cttgccttcc
     2761 tgcctctgct ctcagactct cagtcacagg agggcacagc tggagagggt cttgcggtta
     2821 tggagacaat tcacccctgt attacaagag agggaaactg agcctgcttt tttgtacaaa
     2881 cttgtgatat ccagtgacgt aggaagtcca tccattcaca gcgcttctat aaaggcgcca
     2941 gctgaggcgc ctactactcc aaccgcgact gcagcgagca actgagaaga ctggatagag
     3001 ccggcggttc cgcgaacgag cagtgaccgc gctcccaccc agctctgctc tgcagctccc
     3061 accagtgtct acccctggac cccttgccgg gctttcccca aacttcgagg atccaccggt
     3121 cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     3181 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     3241 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     3301 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
     3361 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     3421 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     3481 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     3541 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     3601 gaagaacggc atcaaggtga acttcaggat ccgccacaac atcgaggacg gcagcgtgca
     3661 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     3721 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     3781 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     3841 caagtaaact agtcgtgagg ctccgatgcc cgtcagtggg cagagcgcac atcgcccaca
     3901 gtccccgaga agttgggggg aggggtcggc aattgaaccg gtgcctagag aaggtggcgc
     3961 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga
     4021 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc
     4081 agaacacagg cgcgccgcta gcccatggtg agcaagggcg aggaggataa catggccatc
     4141 atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc
     4201 gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag
     4261 gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac
     4321 ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc
     4381 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg
     4441 acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc
     4501 aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc
     4561 gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg
     4621 aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg
     4681 cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacttccca caacgaggac
     4741 tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac
     4801 gagctgtaca agtaactcga gctcgcgaaa gcttatcgat aatcaacctc tggattacaa
     4861 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     4921 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     4981 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     5041 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     5101 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     5161 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     5221 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
     5281 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     5341 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     5401 tcggatctcc ctttgggccg cctccccgca tcgataccgt cgactctaga ggatccccgg
     5461 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     5521 aaggggggac tggaagggct aattcactcc caacgaagac aaaatcgtcg agagatgctg
     5581 catataagca gctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     5641 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     5701 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     5761 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt
     5821 atttataact tgcaaagaaa tgaatatcag agagtgagag gccttgacat tataatagat
     5881 ttagcaggaa ttgaactagg agtggagcac acaggcaaag ctgcagaagt acttggaaga
     5941 agccaccaga gatactcacg attctgcaca tacctggcta atcccagatc ctaaggatta
     6001 cattaagttt actaacattt atataatgat ttatagttta aagtataaac ttatctaatt
     6061 tactattctg acagatatta attaatcctc aaatatcata agagatgatt actattatcc
     6121 ccatttaaca caagaggaaa ctgagaggga aagatgttga agtaattttc ccacaattac
     6181 agcatccgtt agttacgact ctatgatctt ctgacacaaa ttccatttac tcctcaccct
     6241 atgactcagt cgaatatatc aaagttatgg acattatgct aagtaacaaa ttaccctttt
     6301 atatagtaaa tactgagtag attgagagaa gaaattgttt gcaaacctga atagcttcaa
     6361 gaagaagaga agtgaggata agaataacag ttgtcattta acaagtttta acaagtaact
     6421 tggttagaaa gggattcaaa tgcataaagc aagggataaa tttttctggc aacaagacta
     6481 tacaatataa ccttaaatat gacttcaaat aattgttgga acttgataaa actaattaaa
     6541 tattattgaa gattatcaat attataaatg taatttactt ttaaaaaggg aacatagaaa
     6601 tgtgtatcat tagagtagaa aacaatcctt attatcacaa tttgtcaaaa caagtttgtt
     6661 attaacacaa gtagaatact gcattcaatt aagttgactg cagattttgt gttttgttaa
     6721 aattagaaag agataacaac aatttgaatt attgaaagta acatgtaaat agttctacat
     6781 acgttctttt gacatcttgt tcaatcattg atcgaagttc tttatcttgg aagaatttgt
     6841 tccaaagact ctgaaataag gaaaacaatc tattatatag tctcacacct ttgttttact
     6901 tttagtgatt tcaatttaat aatgtaaatg gttaaaattt attcttctct gagatcattt
     6961 cacattgcag atagaaaacc tgagactggg gtaattttta ttaaaatcta atttaatctc
     7021 agaaacacat ctttattcta acatcaattt ttccagtttg atattatcat ataaagtcag
     7081 ccttcctcat ctgcaggttc cacaacaaaa atccaaccaa ctgtggatca aaaatattgg
     7141 gaaaaaatta aaaatagcaa tacaacaata aaaaaataca aatcagaaaa acagcacagt
     7201 ataacaactt tatttagcat ttacaatcta ttaggtatta taagtaatct agccagatcc
     7261 tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct
     7321 atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt
     7381 gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct
     7441 tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct
     7501 tcctaatgca ggagtcgcat aagggagagc gtcgaatggt gcactctcag tacaatctgc
     7561 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
     7621 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     7681 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
     7741 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     7801 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     7861 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     7921 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     7981 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     8041 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     8101 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     8161 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     8221 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     8281 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     8341 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     8401 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     8461 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     8521 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     8581 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     8641 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     8701 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     8761 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     8821 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     8881 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     8941 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     9001 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     9061 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     9121 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     9181 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     9241 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     9301 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     9361 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     9421 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     9481 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     9541 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     9601 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     9661 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     9721 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgt
     9781 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     9841 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     9901 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     9961 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
    10021 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
    10081 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct tggacacaag acaggcttgc
    10141 gagatatgtt tgagaatacc actttatccc gcgtcaggga gaggcagtgc gtaaaaagac
    10201 gcggactcat gtgaaatact ggtttttagt gcgccagatc tctataatct cgcgcaacct
    10261 attttcccct cgaacacttt ttaagccgta gataaacagg ctgggacact tcacatgagc
    10321 gaaaaataca tcgtcacctg ggacatgttg cagatccatg cacgtaaact cgcaagccga
    10381 ctgatgcctt ctgaacaatg gaaaggcatt attgccgtaa gccgtggcgg tctgtaccgg
    10441 gtgcgttact ggcgcgtgaa ctgggtattc gtcatgtcga taccgtttgt atttccagct
    10501 acgatcacga caaccagcgc gagcttaaag tgctgaaacg cgcagaaggc gatggcgaag
    10561 gcttcatcgt tattgatgac ctggtggata ccggtggtac tgcggttgcg attcgtgaaa
    10621 tgtatccaaa agcgcacttt gtcaccatct tcgcaaaacc ggctggtcgt ccgctggttg
    10681 atgactatgt tgttgatatc ccgcaagata cctggattga acagccgtgg gatatgggcg
    10741 tcgtattcgt cccgccaatc tccggtcgct aatcttttca acgcctggca ctgccgggcg
    10801 ttgttctttt taacttcagg cgggttacaa tagtttccag taagtattct ggaggctgca
    10861 tccatgacac aggcaaacct gagcgaaacc ctgttcaaac cccgctttaa acatcctgaa
    10921 acctcgacgc tagtccgccg ctttaatcac ggcgcacaac cgcctgtgca gtcggccctt
    10981 gatggtaaaa ccatccctca ctggtatcgc atgattaacc gtctgatgtg gatctggcgc
    11041 ggcattgacc cacgcgaaat cctcgacgtc caggcacgta ttgtgatgag cgatgccgaa
    11101 cgtaccgacg atgatttata cgatacggtg attggctacc gtggcggcaa ctggatttat
    11161 gagtgggccc cggatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa
    11221 actacctaca gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt
    11281 taaactactg attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg
    11341 ggagcagtgg tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc
    11401 tagtgatgat gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa
    11461 ggtagaagac cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt
    11521 tagtaataga actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct
    11581 atacaagaaa attatggaaa aatattctgt aacctttata agtaggcata acagttataa
    11641 tcataacata ctgttttttc ttactccaca caggcataga gtgtctgcta ttaataacta
    11701 tgctcaaaaa ttgtgtacct ttagcttttt aatttgtaaa ggggttaata aggaatattt
    11761 gatgtatagt gccttgacta gagatcataa tcagccatac cacatttgta gaggttttac
    11821 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
    11881 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
    11941 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
    12001 atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac
    12061 ttcccacccc ataccctatt accactgcca attacctgtg gtttca
//
