LOCUS       sequence-226939-        4084 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FtsY gene cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4084)
  AUTHORS   Diebold-Durand ML, Burmann F, Gruber S
  TITLE     High-Throughput Allelic Replacement Screening in Bacillus subtilis.
  JOURNAL   Methods Mol Biol. 2019;2004:49-61. doi: 10.1007/978-1-4939-9520-2_5.
  PUBMED    31147909
REFERENCE   2  (bases 1 to 4084)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2019"; volume: "2004:49-61. doi"; pages: " 
            10.1007/978-1-4939-9520-2_5"
COMMENT     Sequence Label: pJet1.2 ftsY
FEATURES             Location/Qualifiers
     source          1..4084
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     305..324
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        305..323
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(join(1916..1922,1923..1940,1941..1946))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    1961..1982
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2102..2119)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2273..2861)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2353..2372)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3032..3823,3824..3892))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3655..3674
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3893..3997)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4065..4083
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gcccctgcag ccgaattata ttatttttgc caaataattt ttaacaaaag ctctgaagtc
       61 ttcttcattt aaattcttag atgatacttc atctggaaaa ttgtcccaat tagtagcatc
      121 acgctgtgag taagttctaa accatttttt tattgttgta ttatctctaa tcttactact
      181 cgatgagttt tcggtattat ctctattttt aacttggagc aggttccatt cattgttttt
      241 ttcatcatag tgaataaaat caactgcttt aacacttgtg cctgaacacc atatccatcc
      301 ggcgtaatac gactcactat agggagagcg gccgccagat cttccggatg gctcgagttt
      361 ttcagcaaga tggtctcacc tgcgaggaaa gaggttaaaa gatgagcttt tttaaaaaat
      421 taaaagagaa aatcacaaaa cagacagatt ccgtatctga aaagtttaag gatggccttg
      481 aaaaaacaag aaactccttt caaaacaaag tgaatgatct tgtatcccgt taccgtaaag
      541 tggatgagga tttcttcgaa gagcttgaag aggttcttat cagcgcggat gtcggtttta
      601 caaccgttat ggaattaata gatgagctga aaaaagaagt caaacgcaga aatattcaag
      661 atccaaagga agtccagtca gtgatttctg agaaactggt cgagatttat aacagcggag
      721 atgagcaaat ttcagaactg aacatccagg atgggcgttt aaacgtaatc cttctggtag
      781 gtgtaaacgg cgtcgggaaa acaacaacga tcggaaagct tgctcataaa atgaaacaag
      841 aaggaaaatc tgttgtactt gccgccggag acacttttag agcgggagcc attgaacagc
      901 tggaagtatg gggagagcgt acaggagtgc ctgtcattaa gcagacggca ggaagcgatc
      961 cggcggctgt catctacgat gctgttcatg ctgcgaaagc aagaaatgcc gatgtattaa
     1021 tttgtgatac ggcagggcgt ctccaaaaca aagtaaatct catgaaagag cttgaaaaag
     1081 taaaacgtgt tatcgaaaga gaagttcctg aagctccgca tgaggtgctg cttgcccttg
     1141 atgccacgac cggccaaaat gcaatggctc aggcaaaaga attctctaaa gcaacaaatg
     1201 ttaccggcat tgctttaacg aagcttgacg gtacggcaaa aggcggtatc gtccttgcga
     1261 ttcgcaacga gcttcacatc ccggttaaac tagtcggttt aggagaaaaa gttgatgacc
     1321 ttcaggaatt tgatccagaa tcctatgtgt acggactctt ttcagattta gtggaaaaag
     1381 ccgacgatta agaaaaaggc cccaacatct tggggccttt ttctttttta tcttcttact
     1441 tgataaccct aaagttatgg aaataagact aataggagac catctttcta gaagatctcc
     1501 tacaatattc tcagctgcca tggaaaatcg atgttcttct tttattctct caagattttc
     1561 aggctgtata ttaaaactta tattaagaac tatgctaacc acctcatcag gaaccgttgt
     1621 aggtggcgtg ggttttcttg gcaatcgact ctcatgaaaa ctacgagcta aatattcaat
     1681 atgttcctct tgaccaactt tattctgcat tttttttgaa cgaggtttag agcaagcttc
     1741 aggaaactga gacaggaatt ttattaaaaa tttaaatttt gaagaaagtt cagggttaat
     1801 agcatccatt ttttgctttg caagttcctc agcattctta acaaaagacg tctcttttga
     1861 catgtttaaa gtttaaacct cctgtgtgaa attattatcc gctcataatt ccacacatta
     1921 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     1981 taattgcgtt gcgctcactg ccaattgctt tccagtcggg aaacctgtcg tgccagctgc
     2041 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     2101 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     2161 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     2221 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     2281 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     2341 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     2401 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     2461 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     2521 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     2581 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     2641 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     2701 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     2761 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     2821 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     2881 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     2941 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     3001 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     3061 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     3121 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     3181 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     3241 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     3301 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     3361 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     3421 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     3481 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     3541 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     3601 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     3661 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     3721 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     3781 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     3841 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     3901 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     3961 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     4021 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     4081 ggcc
//
