LOCUS       sequence-226940-       15482 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  miniTn7 plasmid to deliver constitutively expressed fluorescent 
            protein genes in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15482)
  AUTHORS   Schlechter RO, Jun H, Bernach M, Oso S, Boyd E, Munoz-Lintz DA, 
            Dobson RCJ, Remus DM, Remus-Emsermann MNP
  TITLE     Chromatic Bacteria - A Broad Host-Range Plasmid and Chromosomal 
            Insertion Toolbox for Fluorescent Protein Expression in Bacteria.
  JOURNAL   Front Microbiol. 2018 Dec 12;9:3052. doi: 10.3389/fmicb.2018.03052. 
            eCollection 2018.
  PUBMED    30631309
REFERENCE   2  (bases 1 to 15482)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol."; date: "2018-12-12"; volume: "9:3052. doi"; pages: " 
            10.3389/fmicb.2018.03052. eCollection 2018"
COMMENT     Sequence Label: pMRE-Tn7-146
FEATURES             Location/Qualifiers
     source          1..15482
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..735
                     /codon_start=1
                     /product="far-red fluorescent protein derived from 
                     mNeptune, with further red-shifted spectra"
                     /label=mCardinal
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTTEGEGKPYEGTQTQ
                     RIKVVEGGPLPFAFDILATCFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTVTQDTSLQDGCLIYNVKLRGVNFPSNGPVMQKKTLGWEATTETLYPADGGLEGRCD
                     MALKLVGGGHLHCNLKTTYRSKKPAKNLKMPGVYFVDRRLERIKEADNETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
     terminator      752..846
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             890..1549
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(956..975)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1614..1700
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(2129..3007)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        3034..3318
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     3241..3260
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     primer_bind     complement(10009..10026)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      10179..10265
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      10357..10384
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        10403..10494
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10495..10563,10564..11355)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10713..10732)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     complement(11484..11503)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     oriT            11526..11635
                     /note="incP origin of transfer"
     primer_bind     complement(11673..11692)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     rep_origin      12375..12597
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             12645..13595
                     /codon_start=1
                     /gene="rep101 encoding an A56V mutation"
                     /product="temperature-sensitive version of the RepA protein
                     needed for replication with the pSC101 origin (Armstrong et
                     al., 1984)"
                     /label=Rep101(Ts)
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYVQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     mobile_element  14028..14193
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     promoter        join(14371..14376,14377..14393,14394..14399)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             14588..15121
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 atggtgagca agggcgagga gctgatcaag gagaacatgc acatgaagct gtacatggaa
       61 ggcaccgtga acaaccacca cttcaagtgc accaccgaag gggagggcaa gccctacgag
      121 ggcacccaga cccagaggat taaggtggtg gagggaggcc ccctgccgtt cgcattcgac
      181 atcctggcca cctgctttat gtacgggagc aagaccttca tcaaccacac ccagggcatc
      241 cccgatttct ttaagcagtc cttccctgag ggcttcacat gggagagagt caccacatac
      301 gaagacgggg gcgtgcttac cgttacccag gacaccagcc tccaggacgg ctgcttgatc
      361 tacaacgtca agctcagagg ggtgaacttc ccatccaacg gccctgtgat gcagaagaaa
      421 acactcggct gggaggccac caccgagacc ctgtaccccg ctgacggcgg cctggaaggc
      481 agatgcgaca tggccctgaa gctcgtgggc gggggccacc tgcactgcaa cctgaagacc
      541 acatacagat ccaagaaacc cgctaagaac ctcaagatgc ccggcgtcta ctttgtggac
      601 cgcagactgg aaagaatcaa ggaggccgac aatgagacct acgtcgagca gcacgaggtg
      661 gctgtggcca gatactgcga cctccctagc aaactggggc acaaacttaa tggcatggac
      721 gagctgtaca agtaataatt agctgagctt ggactcctgt tgatagatcc agtaatgacc
      781 tcagaactcc atctggattt gttcagaacg ctcggttgcc gccgggcgtt ttttattggt
      841 gagaatccaa gctagcttgg cgagattttc aggagctaag gaagctaaaa tggagaaaaa
      901 aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc
      961 atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt
     1021 tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc
     1081 ccgcctgatg aatgctcatc cggaatttcg tatggcaatg aaagacggtg agctggtgat
     1141 atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc
     1201 gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt
     1261 ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt
     1321 cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga
     1381 caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct
     1441 gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat
     1501 gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaat ttttttaagg
     1561 cagttattgg tgcccttaaa cgcctggggt aatgactctc tagcttgagg catcaaataa
     1621 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
     1681 ctctcctgag taggacaaat ccgccgctag gagcttgcgg ccgccagggg tcaatagcgg
     1741 ccgccactcg agcacctagg aggcgcgcca cggccgcgtc gaccccacgc ccctctttaa
     1801 tacgacgggc aatttgcact tcagaaaatg aagagtttgc tttagccata acaaaagtcc
     1861 agtatgcttt ttcacagcat aactggactg atttcagttt acaactattc tgtctagttt
     1921 aagactttat tgtcatagtt tagatctatt ttgttcagtt taagacttta ttgtccgccc
     1981 acaggccacg atgcgtccgg cgtagaggat ctgctcatgt ttgacagctt atcatcgatg
     2041 cataatgtgc ctgtcaaatg gacgaagcag ggattctgca aaccctatgc tactccgtca
     2101 agccgtcaat tgtctgattc gttaccaatt atgacaactt gacggctaca tcattcactt
     2161 tttcttcaca accggcacgg aactcgctcg ggctggcccc ggtgcatttt ttaaataccc
     2221 gcgagaaata gagttgatcg tcaaaaccaa cattgcgacc gacggtggcg ataggcatcc
     2281 gggtggtgct caaaagcagc ttcgcctggc tgatacgttg gtcctcgcgc cagcttaaga
     2341 cgctaatccc taactgctgg cggaaaagat gtgacagacg cgacggcgac aagcaaacat
     2401 gctgtgcgac gctggcgata tcaaaattgc tgtctgccag gtgatcgctg atgtactgac
     2461 aagcctcgcg tacccgatta tccatcggtg gatggagcga ctcgttaatc gcttccatgc
     2521 gccgcagtaa caattgctca agcagattta tcgccagcag ctccgaatag cgcccttccc
     2581 cttgcccggc gttaatgatt tgcccaaaca ggtcgctgaa atgcggctgg tgcgcttcat
     2641 ccgggcgaaa gaaccccgta ttggcaaata ttgacggcca gttaagccat tcatgccagt
     2701 aggcgcgcgg acgaaagtaa acccactggt gataccattc gcgagcctcc ggatgacgac
     2761 cgtagtgatg aatctctcct ggcgggaaca gcaaaatatc acccggtcgg caaacaaatt
     2821 ctcgtccctg atttttcacc accccctgac cgcgaatggt gagattgaga atataacctt
     2881 tcattcccag cggtcggtcg ataaaaaaat cgagataacc gttggcctca atcggcgtta
     2941 aacccgccac cagatgggca ttaaacgagt atcccggcag caggggatca ttttgcgctt
     3001 cagccatact tttcatactc ccgccattca gagaagaaac caattgtcca tattgcatca
     3061 gacattgccg tcactgcgtc ttttactggc tcttctcgct aaccaaaccg gtaaccccgc
     3121 ttattaaaag cattctgtaa caaagcggga ccaaagccat gacaaaaacg cgtaacaaaa
     3181 gtgtctataa tcacggcaga aaagtccaca ttgattattt gcacggcgtc acactttgct
     3241 atgccatagc atttttatcc ataagattag cggatcctac ctgacgcttt ttatcgcaac
     3301 tctctactgt ttctccatac ccgttttttt gggctagcga attcgagctc ggtacccaag
     3361 tcttaaacta gacagaatag ttgtaaactg aaatcagtcc agttatgctg tgaaaaagca
     3421 tactggactt ttgttatggc taaagcaaac tcttcatttt ctgaagtgca aattgcccgt
     3481 cgtattaaag aggggcgtgg ccaagggcat ggtaaagact atattccatg gctaacagta
     3541 caagaagttc cttcttcagg tcgttcccac cgtatttatt ctcataagac gggacgagtc
     3601 catcatttgc tatctgactt agagcttgct gtttttctca gtcttgagtg ggagagcagc
     3661 gtgctagata tacgcgagca gttcccctta ttacctagtg ataccaggca gattgcaata
     3721 gatagtggta ttaagcatcc tgttattcgt ggtgtagatc aggttatgtc tactgatttt
     3781 ttagtggact gcaaagatgg tccttttgag cagtttgcta ttcaagtcaa acctgcagca
     3841 gccttacaag acgagcgtac cttagaaaaa ctagaactag agcgtcgcta ttggcagcaa
     3901 aagcaaattc cttggttcat ttttactgat aaagaaataa atcccgtagt aaaagaaaat
     3961 attgaatggc tttattcagt gaaaacagaa gaagtttctg cggagctttt agcacaacta
     4021 tccccattgg cccatatcct gcaagaaaaa ggagatgaaa acattatcaa tgtctgtaag
     4081 caggttgata ttgcttatga tttggagtta ggcaaaacat tgagtgagat acgagcctta
     4141 accgcaaatg gttttattaa gttcaatatt tataagtctt tcagggcaaa taagtgtgca
     4201 gatctctgta ttagccaagt agtgaatatg gaggagttgc gctatgtggc aaattaatga
     4261 ggttgtgcta tttgataatg atccgtatcg cattttggct atagaggatg gccaagttgt
     4321 ctggatgcaa ataagcgctg ataaaggagt tccacaagct agggctgagt tgttgctaat
     4381 gcagtattta gatgaaggcc gcttagttag aactgatgac ccttatgtac atcttgattt
     4441 agaagagccg tctgtagatt ctgtcagctt ccagaagcgc gaggaggatt atcgaaaaat
     4501 tcttcctatt attaatagta aggatcgttt cgaccctaaa gtcagaagcg aactcgttga
     4561 gcatgtggtc caagaacata aggttactaa ggctacagtt tataagttgt tacgccgtta
     4621 ctggcagcgt ggtcaaacgc ctaatgcatt aattcctgac tacaaaaaca gcggtgcacc
     4681 aggggaaaga cgttcagcga caggaacagc aaagattggc cgagccagag aatatggtaa
     4741 gggtgaagga accaaggtaa cgcccgagat tgaacgcctt tttaggttga ccatagaaaa
     4801 gcacctgtta aatcaaaaag gtacaaagac caccgttgcc tatagacgat ttgtggactt
     4861 gtttgctcag tattttcctc gcattcccca agaggattac ccaacactac gtcagtttcg
     4921 ttatttttat gatcgagaat accctaaagc tcagcgctta aagtctagag ttaaagcagg
     4981 ggtatataaa aaagacgtac gacccttaag tagtacagcc acttctcagg cgttaggccc
     5041 tgggagtcgt tatgagattg atgccacgat tgctgatatt tatttagtgg atcatcatga
     5101 tcgccaaaaa atcataggaa gaccaacgct ttacattgtg attgatgtgt ttagtcggat
     5161 gatcacgggc ttttatatcg gctttgaaaa tccgtcttat gtggtggcga tgcaggcttt
     5221 tgtaaatgct tgctctgaca aaacggccat ttgtgcccag catgatattg agattagtag
     5281 ctcagactgg ccgtgtgtag gtttgccaga tgtgttgcta gcggaccgtg gcgaattaat
     5341 gagtcatcag gtcgaagcct tagtttctag ttttaatgtg cgagtggaaa gtgctccacc
     5401 tagacgtggc gatgctaaag gcatagtgga aagcactttt agaacactac aagccgagtt
     5461 taagtccttt gcacctggca ttgtagaggg cagtcggatc aaaagccatg gtgaaacaga
     5521 ctataggtta gatgcatctc tgtcggtatt tgagttcaca caaattattt tgcgtacgat
     5581 cttattcaga aataaccatc tggtgatgga taaatacgat cgagatgctg attttcctac
     5641 agatttaccg tctattcctg tccagctatg gcaatggggt atgcagcatc gtacaggtag
     5701 tttaagggct gtggagcaag agcagttgcg agtagcgtta ctgcctcgcc gaaaggtctc
     5761 tatttcttca tttggcgtta atttgtgggg tttgtattac tcggggtcag agattctgcg
     5821 tgagggttgg ttgcagcgga gcactgatat agctagacct caacatttag aagcggctta
     5881 tgacccagtg ctggttgata cgatttattt gtttccgcaa gttggcagcc gtgtattttg
     5941 gcgctgtaat ctgacggaac gtagtcggca gtttaaaggt ctctcatttt gggaggtttg
     6001 ggatatacaa gcacaagaaa aacacaataa agccaatgcg aagcaggatg agttaactaa
     6061 acgcagggag cttgaggcgt ttattcagca aaccattcag aaagcgaata agttaacgcc
     6121 cagtactact gagcccaaat caacacgcat taagcagatt aaaactaata aaaaagaagc
     6181 cgtgacctcg gagcgtaaaa aacgtgcgga gcatttgaag ccaagctctt caggtgatga
     6241 ggctaaagtt attcctttca acgcagtgga agcggatgat caagaagatt acagcctacc
     6301 cacatacgtg cctgaattat ttcaggatcc accagaaaag gatgagtcat gagtgctacc
     6361 cggattcaag cagtttatcg tgatacgggg gtagaggctt atcgtgataa tccttttatc
     6421 gaggccttac caccattaca agagtcagtg aatagtgctg catcactgaa atcctcttta
     6481 cagcttactt cctctgactt gcaaaagtcc cgtgttatca gagctcatac catttgtcgt
     6541 attccagatg actattttca gccattaggt acgcatttgc tactaagtga gcgtatttcg
     6601 gtcatgattc gaggtggcta cgtaggcaga aatcctaaaa caggagattt acaaaagcat
     6661 ttacaaaatg gttatgagcg tgttcaaacg ggagagttgg agacatttcg ctttgaggag
     6721 gcacgatcta cggcacaaag cttattgtta attggttgtt ctggtagtgg gaagacgacc
     6781 tctcttcatc gtattctagc cacgtatcct caggtgattt accatcgtga actcaatgta
     6841 gagcaggtgg tgtatttgaa aatagactgc tcgcataatg gttcgctaaa agaaatctgc
     6901 ttgaattttt tcagagcgtt ggatcgagcc ttgggctcga actatgagcg tcgttatggc
     6961 ttaaaacgtc atggtataga aaccatgttg gctttgatgt cgcaaatagc caatgcacat
     7021 gctttagggt tgttggttat tgatgaaatt cagcatttaa gccgctctcg ttcgggtgga
     7081 tctcaagaga tgctgaactt ttttgtgacg atggtgaata ttattggcgt accagtgatg
     7141 ttgattggta cccctaaagc acgagagatt tttgaggctg atttgcggtc tgcacgtaga
     7201 ggggcagggt ttggagctat attctgggat cctatacaac aaacgcaacg tggaaagccc
     7261 aatcaagagt ggatcgcttt tacggataat ctttggcaat tacagctttt acaacgcaaa
     7321 gatgcgctgt tatcggatga ggtccgtgat gtgtggtatg agctaagcca aggagtgatg
     7381 gacattgtag taaaactttt tgtactcgct cagctccgtg cgctagcttt aggcaatgag
     7441 cgtattaccg ctggtttatt gcggcaagtg tatcaagatg agttaaagcc tgtgcacccc
     7501 atgctagagg cattacgctc gggtatccca gaacgcattg ctcgttattc tgatctagtc
     7561 gttcccgaga ttgataaacg gttaatccaa cttcagctag atatcgcagc gatacaagaa
     7621 caaacaccag aagaaaaagc ccttcaagag ttagataccg aagatcagcg tcatttatat
     7681 ctgatgctga aagaggatta cgattcaagc ctgttaattc ccactattaa aaaagcgttt
     7741 agccagaatc caacgatgac aagacaaaag ttactgcctc ttgttttgca gtggttgatg
     7801 gaaggcgaaa cggtagtgtc agaactagaa aagccctcca agagtaaaaa ggtttcggct
     7861 ataaaggtag tcaagcccag cgactgggat agcttgcctg atacggattt acgttatatc
     7921 tattcacaac gccaacctga aaaaaccatg catgaacggt taaaagggaa aggggtaata
     7981 gtggatatgg cgagcttatt taaacaagca ggttagccat gagaaacttt cctgttccgt
     8041 actcgaatga gctgatttat agcactattg cacgggcagg cgtttatcaa gggattgtta
     8101 gtcctaagca gctgttggat gaggtgtatg gcaaccgcaa ggtggtcgct accttaggtc
     8161 tgccctcgca tttaggtgtg atagcaagac atctacatca aacaggacgt tacgctgttc
     8221 agcagcttat ttatgagcat accttattcc ctttatatgc tccgtttgta ggcaaggagc
     8281 gccgagacga agctattcgg ttaatggagt accaagcgca aggtgcggtg catttaatgc
     8341 taggagtcgc tgcttctaga gttaagagcg ataaccgctt tagatactgc cctgattgcg
     8401 ttgctcttca gctaaatagg tatggggaag ccttttggca acgagattgg tatttgcccg
     8461 ctttgccata ttgtccaaaa cacggtgctt tagtcttctt tgatagagct gtagatgatc
     8521 accgacatca attttgggct ttgggtcata ctgagctgct ttcagactac cccaaagact
     8581 ccctatctca attaacagca ctagctgctt atatagcccc tctgttagat gctccacgag
     8641 cgcaagagct ttccccaagc cttgagcagt ggacgctgtt ttatcagcgc ttagcgcagg
     8701 atctagggct aaccaaaagc aagcacattc gtcatgactt ggtggcggag agagtgaggc
     8761 agacttttag tgatgaggca ctagagaaac tggatttaaa gttggcagag aacaaggaca
     8821 cgtgttggct gaaaagtata ttccgtaagc atagaaaagc ctttagttat ttacagcata
     8881 gtattgtgtg gcaagcctta ttgccaaaac taacggttat agaagcgcta cagcaggcaa
     8941 gtgctcttac tgagcactct ataacgacaa gacctgttag ccagtctgtg caacctaact
     9001 ctgaagattt atctgttaag cataaagact ggcagcaact agtgcataaa taccaaggaa
     9061 ttaaggcggc aagacagtct ttagagggtg gggtgctata cgcttggctt taccgacatg
     9121 acagggattg gctagttcac tggaatcaac agcatcaaca agagcgtctg gcacccgccc
     9181 ctagagttga ttggaaccaa agagatcgaa ttgctgtacg acaactatta agaatcataa
     9241 agcgtctaga tagtagcctt gatcacccaa gagcgacatc gagctggctg ttaaagcaaa
     9301 ctcctaacgg aacctctctt gcaaaaaatc tacagaaact gcctttggta gcgctttgct
     9361 taaagcgtta ctcagagagt gtggaagatt atcaaattag acggattagc caagctttta
     9421 ttaagcttaa acaggaagat gttgagctta ggcgctggcg attattaaga agtgcaacgt
     9481 tatctaaaga gcggataact gaggaagcac aaagattctt ggaaatggtt tatggggaag
     9541 agtgagtggt taggctagct acatttaatg acaatgtgca ggttgtacat attggtcatt
     9601 tattccgtaa ctcgggtcat aaggagtggc gtatttttgt ttggtttaat ccaatgcaag
     9661 aacggaaatg gactcgattt actcatttgc ctttattaag tcgagctaag gtggttaaca
     9721 gtacaacaaa gcaaataaat aaggcggatc gtgtgattga gtttgaagca tcggatcttc
     9781 aacgagccaa aataatcgat tttcctaatc tctcgtcctt tgcttccgta cgcaacaagg
     9841 atggagcgca gagttcattt atttacgaag ctgaaacacc atatagcaag actcgttatc
     9901 acatcccaca gttagagcta gctcggtcat tatttttagg ggatcctcta gagtcgacct
     9961 gcaggcatgc aagcttggct gttttggcgg atgagagaag attttcagcc tgatacagat
    10021 taaatcagaa cgcagaagcg gtctgataaa acagaatttg cctggcggca gtagcgcggt
    10081 ggtcccacct gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg atggtagtgt
    10141 ggggtctccc catgcgagag tagggaactg ccaggcatca aataaaacga aaggctcagt
    10201 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga
    10261 caaatccgcc gggagcggat ttgaacgttg cgaagcaacg gcccggaggg tggcgggcag
    10321 gacgcccgcc ataaactgcc aggcatcaaa ttaagcagaa ggccatcctg acggatggcc
    10381 tttttgcgtt tctacaaact cttttgttta tttttctaaa tacattcaaa tatgtatccg
    10441 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
    10501 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
    10561 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
    10621 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
    10681 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt
    10741 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
    10801 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
    10861 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
    10921 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
    10981 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca
    11041 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
    11101 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
    11161 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
    11221 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
    11281 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
    11341 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttacgc
    11401 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
    11461 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
    11521 cgccgccggc cagcctcgca gagcaggatt cccgttgagc accgccaggt gcgaataagg
    11581 gacagtgaag aaggaacacc cgctcgcggg tgggcctact tcacctatcc tgcccggcgg
    11641 catcaccggc gccacaggtg cggttgctgg cgcctatatc gccgacatca ccgatgggga
    11701 agatcgggct cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg
    11761 ccccgtggcc gggggactgt tgggcgccat ctccttgcat gcaccattcc ttgcggcggc
    11821 ggtgctcaac ggcctcaacc tactactggg ctgcttccta atgcaggagt cgcataaggg
    11881 agagcgtcga tccccgacag taagacgggt aagcctgttg atgataccgc tgccttactg
    11941 ggtgcattag ccagtctgaa tgacctgtca cgggataatc cgaagtggtc agactggaaa
    12001 atcagagggc aggaactgct gaacagcaaa aagtcagata gcaccacata gcagacccgc
    12061 cataaaacgc cctgagaagc ccgtgacggg cttttcttgt attatgggta gtttccttgc
    12121 atgaatccat aaaaggcgcc tgtagtgcca tttaccccca ttcactgcca gagccgtgag
    12181 cgcagcgaac tgaatgtcac gaaaaagaca gcgactcagg tgcctgatgg tcggagacaa
    12241 aaggaatatt cagcgatttg cccgagcttg cgagggtgct acttaagcct ttagggtttt
    12301 aaggtctgtt ttgtagagga gcaaacagcg tttgcgacat ccttttgtaa tactgcggaa
    12361 ctgactaaag tagtgagtta tacacagggc tgggatctat tctttttatc tttttttatt
    12421 ctttctttat tctataaatt ataaccactt gaatataaac aaaaaaaaca cacaaaggtc
    12481 tagcggaatt tacagagggt ctagcagaat ttacaagttt tccagcaaag gtctagcaga
    12541 atttacagat acccacaact caaaggaaaa ggactagtaa ttatcattga ctagcccatc
    12601 tcaattggta tagtgattaa aatcacctag accaattgag atgtatgtct gaattagttg
    12661 ttttcaaagc aaatgaacta gcgattagtc gctatgactt aacggagcat gaaaccaagc
    12721 taattttatg ctgtgtggca ctactcaacc ccacgattga aaaccctaca aggaaagaac
    12781 ggacggtatc gttcacttat aaccaatacg ttcagatgat gaacatcagt agggaaaatg
    12841 cttatggtgt attagctaaa gcaaccagag agctgatgac gagaactgtg gaaatcagga
    12901 atcctttggt taaaggcttt gagattttcc agtggacaaa ctatgccaag ttctcaagcg
    12961 aaaaattaga attagttttt agtgaagaga tattgcctta tcttttccag ttaaaaaaat
    13021 tcataaaata taatctggaa catgttaagt cttttgaaaa caaatactct atgaggattt
    13081 atgagtggtt attaaaagaa ctaacacaaa agaaaactca caaggcaaat atagagatta
    13141 gccttgatga atttaagttc atgttaatgc ttgaaaataa ctaccatgag tttaaaaggc
    13201 ttaaccaatg ggttttgaaa ccaataagta aagatttaaa cacttacagc aatatgaaat
    13261 tggtggttga taagcgaggc cgcccgactg atacgttgat tttccaagtt gaactagata
    13321 gacaaatgga tctcgtaacc gaacttgaga acaaccagat aaaaatgaat ggtgacaaaa
    13381 taccaacaac cattacatca gattcctacc tacataacgg actaagaaaa acactacacg
    13441 atgctttaac tgcaaaaatt cagctcacca gttttgaggc aaaatttttg agtgacatgc
    13501 aaagtaagta tgatctcaat ggttcgttct catggctcac gcaaaaacaa cgaaccacac
    13561 tagagaacat actggctaaa tacggaagga tctgaggttc ttatggctct tgtatctatc
    13621 agtgaagcat caagactaac aaacaaaagt agaacaactg ttcaccgtta catatcaaag
    13681 ggaaaactgt ccatatgcac agatgaaaac ggtgtaaaaa agatagatac atcagagctt
    13741 ttacgagttt ttggtgcatt taaagctgtt caccatgaac agatcgacaa tgtaacagat
    13801 gaacagcatg taacacctaa tagaacaggt gaaaccagta aaacaaagca actagaacat
    13861 gaaattgaac acctgagaca acttgttaca gctcaacagt cacacataga cagcctgaaa
    13921 caggcgatgc tgcttatcga atcaaagctg ccgacaacac gggagccagt gacgcctccc
    13981 gtggggaaaa aatcatggca attctggaag aaatagcgct ttcagcctgt gggcggacaa
    14041 aatagttggg aactgggagg ggtggaaatg gagtttttaa ggattattta gggaagagtg
    14101 acaaaataga tgggaactgg gtgtagcgtc gtaagctaat acgaaaatta aaaatgacaa
    14161 aatagtttgg aactagattt cacttatctg gttggtcgac actagtatta ccctgttatc
    14221 cctagattta aatgatatcg gatcctagta agccacgttt taattaatca gatcccataa
    14281 actgccagga attggggatc ggattcgagc tttagtacct agatttagat gtctaaaaag
    14341 cggggtaccg aggacgcgtc aattctcgaa ttgacataag cctgttcggt tcgtaaactg
    14401 taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt
    14461 ggtaacggcg cagtggcggt tttcatggct tgttatgact gtttttttgt acagtctatg
    14521 cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc
    14581 agcaacgatg ttacgcagca gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa
    14641 gttaggtggc tcaagtatgg gcatcattcg cacatgtagg ctcggccctg accaagtcaa
    14701 atccatgcgg gctgctcttg atcttttcgg tcgtgagttc ggagacgtag ccacctactc
    14761 ccaacatcag ccggactccg attacctcgg gaacttgctc cgtagtaaga cattcatcgc
    14821 gcttgctgcc ttcgaccaag aagcggttgt tggcgctctc gcggcttacg ttctgcccaa
    14881 gtttgagcag ccgcgtagtg agatctatat ctatgatctc gcagtctccg gcgagcaccg
    14941 gaggcagggc attgccaccg cgctcatcaa tctcctcaag catgaggcca acgcgcttgg
    15001 tgcttatgtg atctacgtgc aagcagatta cggtgacgat cccgcagtgg ctctctatac
    15061 aaagttgggc atacgggaag aagtgatgca ctttgatatc gacccaagta ccgccaccta
    15121 aagctcgcac gctgccgcaa gcactcaggg cgcaagggct gctaaaggaa gcggaacacg
    15181 tagaaagcca gtccgcagaa acggtgctga ccccggatga atgtcagcta ctgggctatc
    15241 tggacaaggg aaaacgcaag cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg
    15301 cgatagctag actgggcggt tttatggaca gcaagcgaac cggaattgcc agctggggcg
    15361 ccctctggta aggttgggaa gccctgcaaa gtaaactgga tggctttctt gccgccaagg
    15421 atctgatggc gcaggggatc aagatctgat caagagacag gatactagtg gaggaagaaa
    15481 aa
//
