LOCUS       sequence-226942-       14632 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  miniTn7 plasmid to deliver constitutively expressed fluorescent 
            protein genes in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14632)
  AUTHORS   Schlechter RO, Jun H, Bernach M, Oso S, Boyd E, Munoz-Lintz DA, 
            Dobson RCJ, Remus DM, Remus-Emsermann MNP
  TITLE     Chromatic Bacteria - A Broad Host-Range Plasmid and Chromosomal 
            Insertion Toolbox for Fluorescent Protein Expression in Bacteria.
  JOURNAL   Front Microbiol. 2018 Dec 12;9:3052. doi: 10.3389/fmicb.2018.03052. 
            eCollection 2018.
  PUBMED    30631309
REFERENCE   2  (bases 1 to 14632)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol."; date: "2018-12-12"; volume: "9:3052. doi"; pages: " 
            10.3389/fmicb.2018.03052. eCollection 2018"
COMMENT     Sequence Label: pMRE-Tn7-135
FEATURES             Location/Qualifiers
     source          1..14632
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..696
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet-I
                     /note="matures faster than mScarlet"
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(134..152)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     402..421
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      717..811
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             855..1514
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(921..940)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1579..1665
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(2094..2972)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        2999..3283
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     3206..3225
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     primer_bind     complement(9974..9991)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      10144..10230
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      10322..10349
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        10368..10459
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10460..10528,10529..11320)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10678..10697)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     complement(11449..11468)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     oriT            11491..11600
                     /note="incP origin of transfer"
     primer_bind     complement(11638..11657)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     rep_origin      12340..12562
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             12610..13560
                     /codon_start=1
                     /gene="rep101 encoding an A56V mutation"
                     /product="temperature-sensitive version of the RepA protein
                     needed for replication with the pSC101 origin (Armstrong et
                     al., 1984)"
                     /label=Rep101(Ts)
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYVQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     mobile_element  13993..14158
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 atggtgagca agggcgaggc agtgatcaag gagttcatgc ggttcaaggt gcacatggag
       61 ggctccatga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
      121 ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt ctcctgggac
      181 atcctgtccc ctcagttcat gtacggctcc agggccttca tcaagcaccc cgccgacatc
      241 cccgactact ataagcagtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
      301 gaggacggcg gcgccgtgac cgtgacccag gacacctccc tggaggacgg caccctgatc
      361 tacaaggtga agctccgcgg caccaacttc cctcctgacg gccccgtaat gcagaagaag
      421 acaatgggct gggaagcgtc caccgagcgg ttgtaccccg aggacggcgt gctgaagggc
      481 gacattaaga tggccctgcg cctgaaggac ggcggccgct acctggcgga cttcaagacc
      541 acctacaagg ccaagaagcc cgtgcagatg cccggcgcct acaacgtcga ccgcaagttg
      601 gacatcacct cccacaacga ggactacacc gtggtggaac agtacgaacg ctccgagggc
      661 cgccactcca ccggcggcat ggacgagctg tacaagtaag taattagctg agcttggact
      721 cctgttgata gatccagtaa tgacctcaga actccatctg gatttgttca gaacgctcgg
      781 ttgccgccgg gcgtttttta ttggtgagaa tccaagctag cttggcgaga ttttcaggag
      841 ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat atatcccaat
      901 ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc tataaccaga
      961 ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag cacaagtttt
     1021 atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa tttcgtatgg
     1081 caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac accgttttcc
     1141 atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat ttccggcagt
     1201 ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc tatttcccta
     1261 aagggtttat tgagaatatg tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt
     1321 ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc atgggcaaat
     1381 attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat catgccgttt
     1441 gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc gatgagtggc
     1501 agggcggggc gtaatttttt taaggcagtt attggtgccc ttaaacgcct ggggtaatga
     1561 ctctctagct tgaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
     1621 tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gctaggagct
     1681 tgcggccgcc aggggtcaat agcggccgcc actcgagcac ctaggaggcg cgccacggcc
     1741 gcgtcgaccc cacgcccctc tttaatacga cgggcaattt gcacttcaga aaatgaagag
     1801 tttgctttag ccataacaaa agtccagtat gctttttcac agcataactg gactgatttc
     1861 agtttacaac tattctgtct agtttaagac tttattgtca tagtttagat ctattttgtt
     1921 cagtttaaga ctttattgtc cgcccacagg ccacgatgcg tccggcgtag aggatctgct
     1981 catgtttgac agcttatcat cgatgcataa tgtgcctgtc aaatggacga agcagggatt
     2041 ctgcaaaccc tatgctactc cgtcaagccg tcaattgtct gattcgttac caattatgac
     2101 aacttgacgg ctacatcatt cactttttct tcacaaccgg cacggaactc gctcgggctg
     2161 gccccggtgc attttttaaa tacccgcgag aaatagagtt gatcgtcaaa accaacattg
     2221 cgaccgacgg tggcgatagg catccgggtg gtgctcaaaa gcagcttcgc ctggctgata
     2281 cgttggtcct cgcgccagct taagacgcta atccctaact gctggcggaa aagatgtgac
     2341 agacgcgacg gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa attgctgtct
     2401 gccaggtgat cgctgatgta ctgacaagcc tcgcgtaccc gattatccat cggtggatgg
     2461 agcgactcgt taatcgcttc catgcgccgc agtaacaatt gctcaagcag atttatcgcc
     2521 agcagctccg aatagcgccc ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg
     2581 ctgaaatgcg gctggtgcgc ttcatccggg cgaaagaacc ccgtattggc aaatattgac
     2641 ggccagttaa gccattcatg ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac
     2701 cattcgcgag cctccggatg acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa
     2761 atatcacccg gtcggcaaac aaattctcgt ccctgatttt tcaccacccc ctgaccgcga
     2821 atggtgagat tgagaatata acctttcatt cccagcggtc ggtcgataaa aaaatcgaga
     2881 taaccgttgg cctcaatcgg cgttaaaccc gccaccagat gggcattaaa cgagtatccc
     2941 ggcagcaggg gatcattttg cgcttcagcc atacttttca tactcccgcc attcagagaa
     3001 gaaaccaatt gtccatattg catcagacat tgccgtcact gcgtctttta ctggctcttc
     3061 tcgctaacca aaccggtaac cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa
     3121 gccatgacaa aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt ccacattgat
     3181 tatttgcacg gcgtcacact ttgctatgcc atagcatttt tatccataag attagcggat
     3241 cctacctgac gctttttatc gcaactctct actgtttctc catacccgtt tttttgggct
     3301 agcgaattcg agctcggtac ccaagtctta aactagacag aatagttgta aactgaaatc
     3361 agtccagtta tgctgtgaaa aagcatactg gacttttgtt atggctaaag caaactcttc
     3421 attttctgaa gtgcaaattg cccgtcgtat taaagagggg cgtggccaag ggcatggtaa
     3481 agactatatt ccatggctaa cagtacaaga agttccttct tcaggtcgtt cccaccgtat
     3541 ttattctcat aagacgggac gagtccatca tttgctatct gacttagagc ttgctgtttt
     3601 tctcagtctt gagtgggaga gcagcgtgct agatatacgc gagcagttcc ccttattacc
     3661 tagtgatacc aggcagattg caatagatag tggtattaag catcctgtta ttcgtggtgt
     3721 agatcaggtt atgtctactg attttttagt ggactgcaaa gatggtcctt ttgagcagtt
     3781 tgctattcaa gtcaaacctg cagcagcctt acaagacgag cgtaccttag aaaaactaga
     3841 actagagcgt cgctattggc agcaaaagca aattccttgg ttcattttta ctgataaaga
     3901 aataaatccc gtagtaaaag aaaatattga atggctttat tcagtgaaaa cagaagaagt
     3961 ttctgcggag cttttagcac aactatcccc attggcccat atcctgcaag aaaaaggaga
     4021 tgaaaacatt atcaatgtct gtaagcaggt tgatattgct tatgatttgg agttaggcaa
     4081 aacattgagt gagatacgag ccttaaccgc aaatggtttt attaagttca atatttataa
     4141 gtctttcagg gcaaataagt gtgcagatct ctgtattagc caagtagtga atatggagga
     4201 gttgcgctat gtggcaaatt aatgaggttg tgctatttga taatgatccg tatcgcattt
     4261 tggctataga ggatggccaa gttgtctgga tgcaaataag cgctgataaa ggagttccac
     4321 aagctagggc tgagttgttg ctaatgcagt atttagatga aggccgctta gttagaactg
     4381 atgaccctta tgtacatctt gatttagaag agccgtctgt agattctgtc agcttccaga
     4441 agcgcgagga ggattatcga aaaattcttc ctattattaa tagtaaggat cgtttcgacc
     4501 ctaaagtcag aagcgaactc gttgagcatg tggtccaaga acataaggtt actaaggcta
     4561 cagtttataa gttgttacgc cgttactggc agcgtggtca aacgcctaat gcattaattc
     4621 ctgactacaa aaacagcggt gcaccagggg aaagacgttc agcgacagga acagcaaaga
     4681 ttggccgagc cagagaatat ggtaagggtg aaggaaccaa ggtaacgccc gagattgaac
     4741 gcctttttag gttgaccata gaaaagcacc tgttaaatca aaaaggtaca aagaccaccg
     4801 ttgcctatag acgatttgtg gacttgtttg ctcagtattt tcctcgcatt ccccaagagg
     4861 attacccaac actacgtcag tttcgttatt tttatgatcg agaataccct aaagctcagc
     4921 gcttaaagtc tagagttaaa gcaggggtat ataaaaaaga cgtacgaccc ttaagtagta
     4981 cagccacttc tcaggcgtta ggccctggga gtcgttatga gattgatgcc acgattgctg
     5041 atatttattt agtggatcat catgatcgcc aaaaaatcat aggaagacca acgctttaca
     5101 ttgtgattga tgtgtttagt cggatgatca cgggctttta tatcggcttt gaaaatccgt
     5161 cttatgtggt ggcgatgcag gcttttgtaa atgcttgctc tgacaaaacg gccatttgtg
     5221 cccagcatga tattgagatt agtagctcag actggccgtg tgtaggtttg ccagatgtgt
     5281 tgctagcgga ccgtggcgaa ttaatgagtc atcaggtcga agccttagtt tctagtttta
     5341 atgtgcgagt ggaaagtgct ccacctagac gtggcgatgc taaaggcata gtggaaagca
     5401 cttttagaac actacaagcc gagtttaagt cctttgcacc tggcattgta gagggcagtc
     5461 ggatcaaaag ccatggtgaa acagactata ggttagatgc atctctgtcg gtatttgagt
     5521 tcacacaaat tattttgcgt acgatcttat tcagaaataa ccatctggtg atggataaat
     5581 acgatcgaga tgctgatttt cctacagatt taccgtctat tcctgtccag ctatggcaat
     5641 ggggtatgca gcatcgtaca ggtagtttaa gggctgtgga gcaagagcag ttgcgagtag
     5701 cgttactgcc tcgccgaaag gtctctattt cttcatttgg cgttaatttg tggggtttgt
     5761 attactcggg gtcagagatt ctgcgtgagg gttggttgca gcggagcact gatatagcta
     5821 gacctcaaca tttagaagcg gcttatgacc cagtgctggt tgatacgatt tatttgtttc
     5881 cgcaagttgg cagccgtgta ttttggcgct gtaatctgac ggaacgtagt cggcagttta
     5941 aaggtctctc attttgggag gtttgggata tacaagcaca agaaaaacac aataaagcca
     6001 atgcgaagca ggatgagtta actaaacgca gggagcttga ggcgtttatt cagcaaacca
     6061 ttcagaaagc gaataagtta acgcccagta ctactgagcc caaatcaaca cgcattaagc
     6121 agattaaaac taataaaaaa gaagccgtga cctcggagcg taaaaaacgt gcggagcatt
     6181 tgaagccaag ctcttcaggt gatgaggcta aagttattcc tttcaacgca gtggaagcgg
     6241 atgatcaaga agattacagc ctacccacat acgtgcctga attatttcag gatccaccag
     6301 aaaaggatga gtcatgagtg ctacccggat tcaagcagtt tatcgtgata cgggggtaga
     6361 ggcttatcgt gataatcctt ttatcgaggc cttaccacca ttacaagagt cagtgaatag
     6421 tgctgcatca ctgaaatcct ctttacagct tacttcctct gacttgcaaa agtcccgtgt
     6481 tatcagagct cataccattt gtcgtattcc agatgactat tttcagccat taggtacgca
     6541 tttgctacta agtgagcgta tttcggtcat gattcgaggt ggctacgtag gcagaaatcc
     6601 taaaacagga gatttacaaa agcatttaca aaatggttat gagcgtgttc aaacgggaga
     6661 gttggagaca tttcgctttg aggaggcacg atctacggca caaagcttat tgttaattgg
     6721 ttgttctggt agtgggaaga cgacctctct tcatcgtatt ctagccacgt atcctcaggt
     6781 gatttaccat cgtgaactca atgtagagca ggtggtgtat ttgaaaatag actgctcgca
     6841 taatggttcg ctaaaagaaa tctgcttgaa ttttttcaga gcgttggatc gagccttggg
     6901 ctcgaactat gagcgtcgtt atggcttaaa acgtcatggt atagaaacca tgttggcttt
     6961 gatgtcgcaa atagccaatg cacatgcttt agggttgttg gttattgatg aaattcagca
     7021 tttaagccgc tctcgttcgg gtggatctca agagatgctg aacttttttg tgacgatggt
     7081 gaatattatt ggcgtaccag tgatgttgat tggtacccct aaagcacgag agatttttga
     7141 ggctgatttg cggtctgcac gtagaggggc agggtttgga gctatattct gggatcctat
     7201 acaacaaacg caacgtggaa agcccaatca agagtggatc gcttttacgg ataatctttg
     7261 gcaattacag cttttacaac gcaaagatgc gctgttatcg gatgaggtcc gtgatgtgtg
     7321 gtatgagcta agccaaggag tgatggacat tgtagtaaaa ctttttgtac tcgctcagct
     7381 ccgtgcgcta gctttaggca atgagcgtat taccgctggt ttattgcggc aagtgtatca
     7441 agatgagtta aagcctgtgc accccatgct agaggcatta cgctcgggta tcccagaacg
     7501 cattgctcgt tattctgatc tagtcgttcc cgagattgat aaacggttaa tccaacttca
     7561 gctagatatc gcagcgatac aagaacaaac accagaagaa aaagcccttc aagagttaga
     7621 taccgaagat cagcgtcatt tatatctgat gctgaaagag gattacgatt caagcctgtt
     7681 aattcccact attaaaaaag cgtttagcca gaatccaacg atgacaagac aaaagttact
     7741 gcctcttgtt ttgcagtggt tgatggaagg cgaaacggta gtgtcagaac tagaaaagcc
     7801 ctccaagagt aaaaaggttt cggctataaa ggtagtcaag cccagcgact gggatagctt
     7861 gcctgatacg gatttacgtt atatctattc acaacgccaa cctgaaaaaa ccatgcatga
     7921 acggttaaaa gggaaagggg taatagtgga tatggcgagc ttatttaaac aagcaggtta
     7981 gccatgagaa actttcctgt tccgtactcg aatgagctga tttatagcac tattgcacgg
     8041 gcaggcgttt atcaagggat tgttagtcct aagcagctgt tggatgaggt gtatggcaac
     8101 cgcaaggtgg tcgctacctt aggtctgccc tcgcatttag gtgtgatagc aagacatcta
     8161 catcaaacag gacgttacgc tgttcagcag cttatttatg agcatacctt attcccttta
     8221 tatgctccgt ttgtaggcaa ggagcgccga gacgaagcta ttcggttaat ggagtaccaa
     8281 gcgcaaggtg cggtgcattt aatgctagga gtcgctgctt ctagagttaa gagcgataac
     8341 cgctttagat actgccctga ttgcgttgct cttcagctaa ataggtatgg ggaagccttt
     8401 tggcaacgag attggtattt gcccgctttg ccatattgtc caaaacacgg tgctttagtc
     8461 ttctttgata gagctgtaga tgatcaccga catcaatttt gggctttggg tcatactgag
     8521 ctgctttcag actaccccaa agactcccta tctcaattaa cagcactagc tgcttatata
     8581 gcccctctgt tagatgctcc acgagcgcaa gagctttccc caagccttga gcagtggacg
     8641 ctgttttatc agcgcttagc gcaggatcta gggctaacca aaagcaagca cattcgtcat
     8701 gacttggtgg cggagagagt gaggcagact tttagtgatg aggcactaga gaaactggat
     8761 ttaaagttgg cagagaacaa ggacacgtgt tggctgaaaa gtatattccg taagcataga
     8821 aaagccttta gttatttaca gcatagtatt gtgtggcaag ccttattgcc aaaactaacg
     8881 gttatagaag cgctacagca ggcaagtgct cttactgagc actctataac gacaagacct
     8941 gttagccagt ctgtgcaacc taactctgaa gatttatctg ttaagcataa agactggcag
     9001 caactagtgc ataaatacca aggaattaag gcggcaagac agtctttaga gggtggggtg
     9061 ctatacgctt ggctttaccg acatgacagg gattggctag ttcactggaa tcaacagcat
     9121 caacaagagc gtctggcacc cgcccctaga gttgattgga accaaagaga tcgaattgct
     9181 gtacgacaac tattaagaat cataaagcgt ctagatagta gccttgatca cccaagagcg
     9241 acatcgagct ggctgttaaa gcaaactcct aacggaacct ctcttgcaaa aaatctacag
     9301 aaactgcctt tggtagcgct ttgcttaaag cgttactcag agagtgtgga agattatcaa
     9361 attagacgga ttagccaagc ttttattaag cttaaacagg aagatgttga gcttaggcgc
     9421 tggcgattat taagaagtgc aacgttatct aaagagcgga taactgagga agcacaaaga
     9481 ttcttggaaa tggtttatgg ggaagagtga gtggttaggc tagctacatt taatgacaat
     9541 gtgcaggttg tacatattgg tcatttattc cgtaactcgg gtcataagga gtggcgtatt
     9601 tttgtttggt ttaatccaat gcaagaacgg aaatggactc gatttactca tttgccttta
     9661 ttaagtcgag ctaaggtggt taacagtaca acaaagcaaa taaataaggc ggatcgtgtg
     9721 attgagtttg aagcatcgga tcttcaacga gccaaaataa tcgattttcc taatctctcg
     9781 tcctttgctt ccgtacgcaa caaggatgga gcgcagagtt catttattta cgaagctgaa
     9841 acaccatata gcaagactcg ttatcacatc ccacagttag agctagctcg gtcattattt
     9901 ttaggggatc ctctagagtc gacctgcagg catgcaagct tggctgtttt ggcggatgag
     9961 agaagatttt cagcctgata cagattaaat cagaacgcag aagcggtctg ataaaacaga
    10021 atttgcctgg cggcagtagc gcggtggtcc cacctgaccc catgccgaac tcagaagtga
    10081 aacgccgtag cgccgatggt agtgtggggt ctccccatgc gagagtaggg aactgccagg
    10141 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
    10201 tcggtgaacg ctctcctgag taggacaaat ccgccgggag cggatttgaa cgttgcgaag
    10261 caacggcccg gagggtggcg ggcaggacgc ccgccataaa ctgccaggca tcaaattaag
    10321 cagaaggcca tcctgacgga tggccttttt gcgtttctac aaactctttt gtttattttt
    10381 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
    10441 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
    10501 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
    10561 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
    10621 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
    10681 atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca
    10741 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
    10801 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
    10861 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
    10921 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
    10981 cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg
    11041 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
    11101 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
    11161 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
    11221 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
    11281 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
    11341 atatatactt tagattgatt tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
    11401 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
    11461 tcttcccttc ctttctcgcc acgttcgccg ccggccagcc tcgcagagca ggattcccgt
    11521 tgagcaccgc caggtgcgaa taagggacag tgaagaagga acacccgctc gcgggtgggc
    11581 ctacttcacc tatcctgccc ggcggcatca ccggcgccac aggtgcggtt gctggcgcct
    11641 atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt
    11701 gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct
    11761 tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct
    11821 tcctaatgca ggagtcgcat aagggagagc gtcgatcccc gacagtaaga cgggtaagcc
    11881 tgttgatgat accgctgcct tactgggtgc attagccagt ctgaatgacc tgtcacggga
    11941 taatccgaag tggtcagact ggaaaatcag agggcaggaa ctgctgaaca gcaaaaagtc
    12001 agatagcacc acatagcaga cccgccataa aacgccctga gaagcccgtg acgggctttt
    12061 cttgtattat gggtagtttc cttgcatgaa tccataaaag gcgcctgtag tgccatttac
    12121 ccccattcac tgccagagcc gtgagcgcag cgaactgaat gtcacgaaaa agacagcgac
    12181 tcaggtgcct gatggtcgga gacaaaagga atattcagcg atttgcccga gcttgcgagg
    12241 gtgctactta agcctttagg gttttaaggt ctgttttgta gaggagcaaa cagcgtttgc
    12301 gacatccttt tgtaatactg cggaactgac taaagtagtg agttatacac agggctggga
    12361 tctattcttt ttatcttttt ttattctttc tttattctat aaattataac cacttgaata
    12421 taaacaaaaa aaacacacaa aggtctagcg gaatttacag agggtctagc agaatttaca
    12481 agttttccag caaaggtcta gcagaattta cagataccca caactcaaag gaaaaggact
    12541 agtaattatc attgactagc ccatctcaat tggtatagtg attaaaatca cctagaccaa
    12601 ttgagatgta tgtctgaatt agttgttttc aaagcaaatg aactagcgat tagtcgctat
    12661 gacttaacgg agcatgaaac caagctaatt ttatgctgtg tggcactact caaccccacg
    12721 attgaaaacc ctacaaggaa agaacggacg gtatcgttca cttataacca atacgttcag
    12781 atgatgaaca tcagtaggga aaatgcttat ggtgtattag ctaaagcaac cagagagctg
    12841 atgacgagaa ctgtggaaat caggaatcct ttggttaaag gctttgagat tttccagtgg
    12901 acaaactatg ccaagttctc aagcgaaaaa ttagaattag tttttagtga agagatattg
    12961 ccttatcttt tccagttaaa aaaattcata aaatataatc tggaacatgt taagtctttt
    13021 gaaaacaaat actctatgag gatttatgag tggttattaa aagaactaac acaaaagaaa
    13081 actcacaagg caaatataga gattagcctt gatgaattta agttcatgtt aatgcttgaa
    13141 aataactacc atgagtttaa aaggcttaac caatgggttt tgaaaccaat aagtaaagat
    13201 ttaaacactt acagcaatat gaaattggtg gttgataagc gaggccgccc gactgatacg
    13261 ttgattttcc aagttgaact agatagacaa atggatctcg taaccgaact tgagaacaac
    13321 cagataaaaa tgaatggtga caaaatacca acaaccatta catcagattc ctacctacat
    13381 aacggactaa gaaaaacact acacgatgct ttaactgcaa aaattcagct caccagtttt
    13441 gaggcaaaat ttttgagtga catgcaaagt aagtatgatc tcaatggttc gttctcatgg
    13501 ctcacgcaaa aacaacgaac cacactagag aacatactgg ctaaatacgg aaggatctga
    13561 ggttcttatg gctcttgtat ctatcagtga agcatcaaga ctaacaaaca aaagtagaac
    13621 aactgttcac cgttacatat caaagggaaa actgtccata tgcacagatg aaaacggtgt
    13681 aaaaaagata gatacatcag agcttttacg agtttttggt gcatttaaag ctgttcacca
    13741 tgaacagatc gacaatgtaa cagatgaaca gcatgtaaca cctaatagaa caggtgaaac
    13801 cagtaaaaca aagcaactag aacatgaaat tgaacacctg agacaacttg ttacagctca
    13861 acagtcacac atagacagcc tgaaacaggc gatgctgctt atcgaatcaa agctgccgac
    13921 aacacgggag ccagtgacgc ctcccgtggg gaaaaaatca tggcaattct ggaagaaata
    13981 gcgctttcag cctgtgggcg gacaaaatag ttgggaactg ggaggggtgg aaatggagtt
    14041 tttaaggatt atttagggaa gagtgacaaa atagatggga actgggtgta gcgtcgtaag
    14101 ctaatacgaa aattaaaaat gacaaaatag tttggaacta gatttcactt atctggttgg
    14161 tcgacactag tattaccctg ttatccctag atttaaatga tatcggatcc tagtaagcca
    14221 cgttttaatt aatcagatcc ctaaactgcc aggaattggg gatcggattc gagctcgcac
    14281 gctgccgcaa gcactcaggg cgcaagggct gctaaaggaa gcggaacacg tagaaagcca
    14341 gtccgcagaa acggtgctga ccccggatga atgtcagcta ctgggctatc tggacaaggg
    14401 aaaacgcaag cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg cgatagctag
    14461 actgggcggt cttatggaca gcaagcgaac cggaattgcc agctggggcg ccctctggta
    14521 aggttgggaa gccctgcaaa gtaaactgga tggctttctt gccgccaagg atctgatggc
    14581 gcaggggatc aagatctgat caagagacag gatactagtg gaggaagaaa aa
//
