LOCUS       sequence-226945-       14654 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  miniTn7 plasmid to deliver constitutively expressed fluorescent 
            protein genes in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14654)
  AUTHORS   Schlechter RO, Jun H, Bernach M, Oso S, Boyd E, Munoz-Lintz DA, 
            Dobson RCJ, Remus DM, Remus-Emsermann MNP
  TITLE     Chromatic Bacteria - A Broad Host-Range Plasmid and Chromosomal 
            Insertion Toolbox for Fluorescent Protein Expression in Bacteria.
  JOURNAL   Front Microbiol. 2018 Dec 12;9:3052. doi: 10.3389/fmicb.2018.03052. 
            eCollection 2018.
  PUBMED    30631309
REFERENCE   2  (bases 1 to 14654)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol."; date: "2018-12-12"; volume: "9:3052. doi"; pages: " 
            10.3389/fmicb.2018.03052. eCollection 2018"
COMMENT     Sequence Label: pMRE-Tn7-133
FEATURES             Location/Qualifiers
     source          1..14654
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(1..3,4..6,7..717)
                     /codon_start=1
                     /product="monomeric optimized variant of EYFP, identical to
                     mVenus but with L68V (Kremers et al., 2006)"
                     /label=SYFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(46..67)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(307..326)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     654..675
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      738..832
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             876..1535
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(942..961)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1600..1686
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     mobile_element  1797..1962
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     CDS             complement(2395..3345)
                     /codon_start=1
                     /gene="rep101 encoding an A56V mutation"
                     /product="temperature-sensitive version of the RepA protein
                     needed for replication with the pSC101 origin (Armstrong et
                     al., 1984)"
                     /label=Rep101(Ts)
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYVQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      3393..3615
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     primer_bind     4298..4317
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     oriT            4355..4464
                     /note="incP origin of transfer"
     primer_bind     4487..4506
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             complement(join(4635..5426,5427..5495))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5258..5277
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5496..5587)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      complement(5606..5633)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5725..5811)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     5964..5981
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     promoter        complement(12672..12956)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(12730..12749)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             12983..13861
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
       61 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      121 ggcaagctga ccctgaagct gatctgcacc accggcaagc tgcccgtgcc ctggcccacc
      181 ctcgtgacca ccctgggcta cggcgtgcag tgcttcgccc gctaccccga ccacatgaag
      241 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
      301 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      361 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      421 aagctggagt acaactacaa cagccacaac gtctatatca ccgccgacaa gcagaagaac
      481 ggcatcaagg ccaacttcaa gatccgccac aacatcgagg acggcggcgt gcagctcgcc
      541 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
      601 tacctgagct accagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc
      661 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa
      721 gtaattagct gagcttggac tcctgttgat agatccagta atgacctcag aactccatct
      781 ggatttgttc agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaagcta
      841 gcttggcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata
      901 ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg
      961 ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa
     1021 agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc ctgatgaatg
     1081 ctcatccgga atttcgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc
     1141 acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat
     1201 accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg tgttacggtg
     1261 aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtc tcagccaatc
     1321 cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac ttcttcgccc
     1381 ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg ccgctggcga
     1441 ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt aatgaattac
     1501 aacagtactg cgatgagtgg cagggcgggg cgtaattttt ttaaggcagt tattggtgcc
     1561 cttaaacgcc tggggtaatg actctctagc ttgaggcatc aaataaaacg aaaggctcag
     1621 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg
     1681 acaaatccgc cgctaggagc ttgcggccgc caggggatct gattaattaa aacgtggctt
     1741 actaggatcc gatatcattt aaatctaggg ataacagggt aatactagtg tcgaccaacc
     1801 agataagtga aatctagttc caaactattt tgtcattttt aattttcgta ttagcttacg
     1861 acgctacacc cagttcccat ctattttgtc actcttccct aaataatcct taaaaactcc
     1921 atttccaccc ctcccagttc ccaactattt tgtccgccca caggctgaaa gcgctatttc
     1981 ttccagaatt gccatgattt tttccccacg ggaggcgtca ctggctcccg tgttgtcggc
     2041 agctttgatt cgataagcag catcgcctgt ttcaggctgt ctatgtgtga ctgttgagct
     2101 gtaacaagtt gtctcaggtg ttcaatttca tgttctagtt gctttgtttt actggtttca
     2161 cctgttctat taggtgttac atgctgttca tctgttacat tgtcgatctg ttcatggtga
     2221 acagctttaa atgcaccaaa aactcgtaaa agctctgatg tatctatctt ttttacaccg
     2281 ttttcatctg tgcatatgga cagttttccc tttgatatgt aacggtgaac agttgttcta
     2341 cttttgtttg ttagtcttga tgcttcactg atagatacaa gagccataag aacctcagat
     2401 ccttccgtat ttagccagta tgttctctag tgtggttcgt tgtttttgcg tgagccatga
     2461 gaacgaacca ttgagatcat acttactttg catgtcactc aaaaattttg cctcaaaact
     2521 ggtgagctga atttttgcag ttaaagcatc gtgtagtgtt tttcttagtc cgttatgtag
     2581 gtaggaatct gatgtaatgg ttgttggtat tttgtcacca ttcattttta tctggttgtt
     2641 ctcaagttcg gttacgagat ccatttgtct atctagttca acttggaaaa tcaacgtatc
     2701 agtcgggcgg cctcgcttat caaccaccaa tttcatattg ctgtaagtgt ttaaatcttt
     2761 acttattggt ttcaaaaccc attggttaag ccttttaaac tcatggtagt tattttcaag
     2821 cattaacatg aacttaaatt catcaaggct aatctctata tttgccttgt gagttttctt
     2881 ttgtgttagt tcttttaata accactcata aatcctcata gagtatttgt tttcaaaaga
     2941 cttaacatgt tccagattat attttatgaa tttttttaac tggaaaagat aaggcaatat
     3001 ctcttcacta aaaactaatt ctaatttttc gcttgagaac ttggcatagt ttgtccactg
     3061 gaaaatctca aagcctttaa ccaaaggatt cctgatttcc acagttctcg tcatcagctc
     3121 tctggttgct ttagctaata caccataagc attttcccta ctgatgttca tcatctgaac
     3181 gtattggtta taagtgaacg ataccgtccg ttctttcctt gtagggtttt caatcgtggg
     3241 gttgagtagt gccacacagc ataaaattag cttggtttca tgctccgtta agtcatagcg
     3301 actaatcgct agttcatttg ctttgaaaac aactaattca gacatacatc tcaattggtc
     3361 taggtgattt taatcactat accaattgag atgggctagt caatgataat tactagtcct
     3421 tttcctttga gttgtgggta tctgtaaatt ctgctagacc tttgctggaa aacttgtaaa
     3481 ttctgctaga ccctctgtaa attccgctag acctttgtgt gttttttttg tttatattca
     3541 agtggttata atttatagaa taaagaaaga ataaaaaaag ataaaaagaa tagatcccag
     3601 ccctgtgtat aactcactac tttagtcagt tccgcagtat tacaaaagga tgtcgcaaac
     3661 gctgtttgct cctctacaaa acagacctta aaaccctaaa ggcttaagta gcaccctcgc
     3721 aagctcgggc aaatcgctga atattccttt tgtctccgac catcaggcac ctgagtcgct
     3781 gtctttttcg tgacattcag ttcgctgcgc tcacggctct ggcagtgaat gggggtaaat
     3841 ggcactacag gcgcctttta tggattcatg caaggaaact acccataata caagaaaagc
     3901 ccgtcacggg cttctcaggg cgttttatgg cgggtctgct atgtggtgct atctgacttt
     3961 ttgctgttca gcagttcctg ccctctgatt ttccagtctg accacttcgg attatcccgt
     4021 gacaggtcat tcagactggc taatgcaccc agtaaggcag cggtatcatc aacaggctta
     4081 cccgtcttac tgtcggggat cgacgctctc ccttatgcga ctcctgcatt aggaagcagc
     4141 ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga
     4201 tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc
     4261 tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc
     4321 cagcaaccgc acctgtggcg ccggtgatgc cgccgggcag gataggtgaa gtaggcccac
     4381 ccgcgagcgg gtgttccttc ttcactgtcc cttattcgca cctggcggtg ctcaacggga
     4441 atcctgctct gcgaggctgg ccggcggcga acgtggcgag aaaggaaggg aagaaagcga
     4501 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
     4561 ccgccgcgct taatgcgccg ctacagggcg cgtaaatcaa tctaaagtat atatgagtaa
     4621 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     4681 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     4741 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     4801 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     4861 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     4921 aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt
     4981 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     5041 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     5101 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     5161 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     5221 cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga
     5281 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     5341 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     5401 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     5461 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     5521 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     5581 aaacaaaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct tctgcttaat
     5641 ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc gttgcttcgc
     5701 aacgttcaaa tccgctcccg gcggatttgt cctactcagg agagcgttca ccgacaaaca
     5761 acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt gatgcctggc
     5821 agttccctac tctcgcatgg ggagacccca cactaccatc ggcgctacgg cgtttcactt
     5881 ctgagttcgg catggggtca ggtgggacca ccgcgctact gccgccaggc aaattctgtt
     5941 ttatcagacc gcttctgcgt tctgatttaa tctgtatcag gctgaaaatc ttctctcatc
     6001 cgccaaaaca gccaagcttg catgcctgca ggtcgactct agaggatccc ctaaaaataa
     6061 tgaccgagct agctctaact gtgggatgtg ataacgagtc ttgctatatg gtgtttcagc
     6121 ttcgtaaata aatgaactct gcgctccatc cttgttgcgt acggaagcaa aggacgagag
     6181 attaggaaaa tcgattattt tggctcgttg aagatccgat gcttcaaact caatcacacg
     6241 atccgcctta tttatttgct ttgttgtact gttaaccacc ttagctcgac ttaataaagg
     6301 caaatgagta aatcgagtcc atttccgttc ttgcattgga ttaaaccaaa caaaaatacg
     6361 ccactcctta tgacccgagt tacggaataa atgaccaata tgtacaacct gcacattgtc
     6421 attaaatgta gctagcctaa ccactcactc ttccccataa accatttcca agaatctttg
     6481 tgcttcctca gttatccgct ctttagataa cgttgcactt cttaataatc gccagcgcct
     6541 aagctcaaca tcttcctgtt taagcttaat aaaagcttgg ctaatccgtc taatttgata
     6601 atcttccaca ctctctgagt aacgctttaa gcaaagcgct accaaaggca gtttctgtag
     6661 attttttgca agagaggttc cgttaggagt ttgctttaac agccagctcg atgtcgctct
     6721 tgggtgatca aggctactat ctagacgctt tatgattctt aatagttgtc gtacagcaat
     6781 tcgatctctt tggttccaat caactctagg ggcgggtgcc agacgctctt gttgatgctg
     6841 ttgattccag tgaactagcc aatccctgtc atgtcggtaa agccaagcgt atagcacccc
     6901 accctctaaa gactgtcttg ccgccttaat tccttggtat ttatgcacta gttgctgcca
     6961 gtctttatgc ttaacagata aatcttcaga gttaggttgc acagactggc taacaggtct
     7021 tgtcgttata gagtgctcag taagagcact tgcctgctgt agcgcttcta taaccgttag
     7081 ttttggcaat aaggcttgcc acacaatact atgctgtaaa taactaaagg cttttctatg
     7141 cttacggaat atacttttca gccaacacgt gtccttgttc tctgccaact ttaaatccag
     7201 tttctctagt gcctcatcac taaaagtctg cctcactctc tccgccacca agtcatgacg
     7261 aatgtgcttg cttttggtta gccctagatc ctgcgctaag cgctgataaa acagcgtcca
     7321 ctgctcaagg cttggggaaa gctcttgcgc tcgtggagca tctaacagag gggctatata
     7381 agcagctagt gctgttaatt gagataggga gtctttgggg tagtctgaaa gcagctcagt
     7441 atgacccaaa gcccaaaatt gatgtcggtg atcatctaca gctctatcaa agaagactaa
     7501 agcaccgtgt tttggacaat atggcaaagc gggcaaatac caatctcgtt gccaaaaggc
     7561 ttccccatac ctatttagct gaagagcaac gcaatcaggg cagtatctaa agcggttatc
     7621 gctcttaact ctagaagcag cgactcctag cattaaatgc accgcacctt gcgcttggta
     7681 ctccattaac cgaatagctt cgtctcggcg ctccttgcct acaaacggag catataaagg
     7741 gaataaggta tgctcataaa taagctgctg aacagcgtaa cgtcctgttt gatgtagatg
     7801 tcttgctatc acacctaaat gcgagggcag acctaaggta gcgaccacct tgcggttgcc
     7861 atacacctca tccaacagct gcttaggact aacaatccct tgataaacgc ctgcccgtgc
     7921 aatagtgcta taaatcagct cattcgagta cggaacagga aagtttctca tggctaacct
     7981 gcttgtttaa ataagctcgc catatccact attacccctt tcccttttaa ccgttcatgc
     8041 atggtttttt caggttggcg ttgtgaatag atataacgta aatccgtatc aggcaagcta
     8101 tcccagtcgc tgggcttgac tacctttata gccgaaacct ttttactctt ggagggcttt
     8161 tctagttctg acactaccgt ttcgccttcc atcaaccact gcaaaacaag aggcagtaac
     8221 ttttgtcttg tcatcgttgg attctggcta aacgcttttt taatagtggg aattaacagg
     8281 cttgaatcgt aatcctcttt cagcatcaga tataaatgac gctgatcttc ggtatctaac
     8341 tcttgaaggg ctttttcttc tggtgtttgt tcttgtatcg ctgcgatatc tagctgaagt
     8401 tggattaacc gtttatcaat ctcgggaacg actagatcag aataacgagc aatgcgttct
     8461 gggatacccg agcgtaatgc ctctagcatg gggtgcacag gctttaactc atcttgatac
     8521 acttgccgca ataaaccagc ggtaatacgc tcattgccta aagctagcgc acggagctga
     8581 gcgagtacaa aaagttttac tacaatgtcc atcactcctt ggcttagctc ataccacaca
     8641 tcacggacct catccgataa cagcgcatct ttgcgttgta aaagctgtaa ttgccaaaga
     8701 ttatccgtaa aagcgatcca ctcttgattg ggctttccac gttgcgtttg ttgtatagga
     8761 tcccagaata tagctccaaa ccctgcccct ctacgtgcag accgcaaatc agcctcaaaa
     8821 atctctcgtg ctttaggggt accaatcaac atcactggta cgccaataat attcaccatc
     8881 gtcacaaaaa agttcagcat ctcttgagat ccacccgaac gagagcggct taaatgctga
     8941 atttcatcaa taaccaacaa ccctaaagca tgtgcattgg ctatttgcga catcaaagcc
     9001 aacatggttt ctataccatg acgttttaag ccataacgac gctcatagtt cgagcccaag
     9061 gctcgatcca acgctctgaa aaaattcaag cagatttctt ttagcgaacc attatgcgag
     9121 cagtctattt tcaaatacac cacctgctct acattgagtt cacgatggta aatcacctga
     9181 ggatacgtgg ctagaatacg atgaagagag gtcgtcttcc cactaccaga acaaccaatt
     9241 aacaataagc tttgtgccgt agatcgtgcc tcctcaaagc gaaatgtctc caactctccc
     9301 gtttgaacac gctcataacc attttgtaaa tgcttttgta aatctcctgt tttaggattt
     9361 ctgcctacgt agccacctcg aatcatgacc gaaatacgct cacttagtag caaatgcgta
     9421 cctaatggct gaaaatagtc atctggaata cgacaaatgg tatgagctct gataacacgg
     9481 gacttttgca agtcagagga agtaagctgt aaagaggatt tcagtgatgc agcactattc
     9541 actgactctt gtaatggtgg taaggcctcg ataaaaggat tatcacgata agcctctacc
     9601 cccgtatcac gataaactgc ttgaatccgg gtagcactca tgactcatcc ttttctggtg
     9661 gatcctgaaa taattcaggc acgtatgtgg gtaggctgta atcttcttga tcatccgctt
     9721 ccactgcgtt gaaaggaata actttagcct catcacctga agagcttggc ttcaaatgct
     9781 ccgcacgttt tttacgctcc gaggtcacgg cttctttttt attagtttta atctgcttaa
     9841 tgcgtgttga tttgggctca gtagtactgg gcgttaactt attcgctttc tgaatggttt
     9901 gctgaataaa cgcctcaagc tccctgcgtt tagttaactc atcctgcttc gcattggctt
     9961 tattgtgttt ttcttgtgct tgtatatccc aaacctccca aaatgagaga cctttaaact
    10021 gccgactacg ttccgtcaga ttacagcgcc aaaatacacg gctgccaact tgcggaaaca
    10081 aataaatcgt atcaaccagc actgggtcat aagccgcttc taaatgttga ggtctagcta
    10141 tatcagtgct ccgctgcaac caaccctcac gcagaatctc tgaccccgag taatacaaac
    10201 cccacaaatt aacgccaaat gaagaaatag agacctttcg gcgaggcagt aacgctactc
    10261 gcaactgctc ttgctccaca gcccttaaac tacctgtacg atgctgcata ccccattgcc
    10321 atagctggac aggaatagac ggtaaatctg taggaaaatc agcatctcga tcgtatttat
    10381 ccatcaccag atggttattt ctgaataaga tcgtacgcaa aataatttgt gtgaactcaa
    10441 ataccgacag agatgcatct aacctatagt ctgtttcacc atggcttttg atccgactgc
    10501 cctctacaat gccaggtgca aaggacttaa actcggcttg tagtgttcta aaagtgcttt
    10561 ccactatgcc tttagcatcg ccacgtctag gtggagcact ttccactcgc acattaaaac
    10621 tagaaactaa ggcttcgacc tgatgactca ttaattcgcc acggtccgct agcaacacat
    10681 ctggcaaacc tacacacggc cagtctgagc tactaatctc aatatcatgc tgggcacaaa
    10741 tggccgtttt gtcagagcaa gcatttacaa aagcctgcat cgccaccaca taagacggat
    10801 tttcaaagcc gatataaaag cccgtgatca tccgactaaa cacatcaatc acaatgtaaa
    10861 gcgttggtct tcctatgatt ttttggcgat catgatgatc cactaaataa atatcagcaa
    10921 tcgtggcatc aatctcataa cgactcccag ggcctaacgc ctgagaagtg gctgtactac
    10981 ttaagggtcg tacgtctttt ttatataccc ctgctttaac tctagacttt aagcgctgag
    11041 ctttagggta ttctcgatca taaaaataac gaaactgacg tagtgttggg taatcctctt
    11101 ggggaatgcg aggaaaatac tgagcaaaca agtccacaaa tcgtctatag gcaacggtgg
    11161 tctttgtacc tttttgattt aacaggtgct tttctatggt caacctaaaa aggcgttcaa
    11221 tctcgggcgt taccttggtt ccttcaccct taccatattc tctggctcgg ccaatctttg
    11281 ctgttcctgt cgctgaacgt ctttcccctg gtgcaccgct gtttttgtag tcaggaatta
    11341 atgcattagg cgtttgacca cgctgccagt aacggcgtaa caacttataa actgtagcct
    11401 tagtaacctt atgttcttgg accacatgct caacgagttc gcttctgact ttagggtcga
    11461 aacgatcctt actattaata ataggaagaa tttttcgata atcctcctcg cgcttctgga
    11521 agctgacaga atctacagac ggctcttcta aatcaagatg tacataaggg tcatcagttc
    11581 taactaagcg gccttcatct aaatactgca ttagcaacaa ctcagcccta gcttgtggaa
    11641 ctcctttatc agcgcttatt tgcatccaga caacttggcc atcctctata gccaaaatgc
    11701 gatacggatc attatcaaat agcacaacct cattaatttg ccacatagcg caactcctcc
    11761 atattcacta cttggctaat acagagatct gcacacttat ttgccctgaa agacttataa
    11821 atattgaact taataaaacc atttgcggtt aaggctcgta tctcactcaa tgttttgcct
    11881 aactccaaat cataagcaat atcaacctgc ttacagacat tgataatgtt ttcatctcct
    11941 ttttcttgca ggatatgggc caatggggat agttgtgcta aaagctccgc agaaacttct
    12001 tctgttttca ctgaataaag ccattcaata ttttctttta ctacgggatt tatttcttta
    12061 tcagtaaaaa tgaaccaagg aatttgcttt tgctgccaat agcgacgctc tagttctagt
    12121 ttttctaagg tacgctcgtc ttgtaaggct gctgcaggtt tgacttgaat agcaaactgc
    12181 tcaaaaggac catctttgca gtccactaaa aaatcagtag acataacctg atctacacca
    12241 cgaataacag gatgcttaat accactatct attgcaatct gcctggtatc actaggtaat
    12301 aaggggaact gctcgcgtat atctagcacg ctgctctccc actcaagact gagaaaaaca
    12361 gcaagctcta agtcagatag caaatgatgg actcgtcccg tcttatgaga ataaatacgg
    12421 tgggaacgac ctgaagaagg aacttcttgt actgttagcc atggaatata gtctttacca
    12481 tgcccttggc cacgcccctc tttaatacga cgggcaattt gcacttcaga aaatgaagag
    12541 tttgctttag ccataacaaa agtccagtat gctttttcac agcataactg gactgatttc
    12601 agtttacaac tattctgtct agtttaagac ttgggtaccg agctcgaatt cgctagccca
    12661 aaaaaacggg tatggagaaa cagtagagag ttgcgataaa aagcgtcagg taggatccgc
    12721 taatcttatg gataaaaatg ctatggcata gcaaagtgtg acgccgtgca aataatcaat
    12781 gtggactttt ctgccgtgat tatagacact tttgttacgc gtttttgtca tggctttggt
    12841 cccgctttgt tacagaatgc ttttaataag cggggttacc ggtttggtta gcgagaagag
    12901 ccagtaaaag acgcagtgac ggcaatgtct gatgcaatat ggacaattgg tttcttctct
    12961 gaatggcggg agtatgaaaa gtatggctga agcgcaaaat gatcccctgc tgccgggata
    13021 ctcgtttaat gcccatctgg tggcgggttt aacgccgatt gaggccaacg gttatctcga
    13081 tttttttatc gaccgaccgc tgggaatgaa aggttatatt ctcaatctca ccattcgcgg
    13141 tcagggggtg gtgaaaaatc agggacgaga atttgtttgc cgaccgggtg atattttgct
    13201 gttcccgcca ggagagattc atcactacgg tcgtcatccg gaggctcgcg aatggtatca
    13261 ccagtgggtt tactttcgtc cgcgcgccta ctggcatgaa tggcttaact ggccgtcaat
    13321 atttgccaat acggggttct ttcgcccgga tgaagcgcac cagccgcatt tcagcgacct
    13381 gtttgggcaa atcattaacg ccgggcaagg ggaagggcgc tattcggagc tgctggcgat
    13441 aaatctgctt gagcaattgt tactgcggcg catggaagcg attaacgagt cgctccatcc
    13501 accgatggat aatcgggtac gcgaggcttg tcagtacatc agcgatcacc tggcagacag
    13561 caattttgat atcgccagcg tcgcacagca tgtttgcttg tcgccgtcgc gtctgtcaca
    13621 tcttttccgc cagcagttag ggattagcgt cttaagctgg cgcgaggacc aacgtatcag
    13681 ccaggcgaag ctgcttttga gcaccacccg gatgcctatc gccaccgtcg gtcgcaatgt
    13741 tggttttgac gatcaactct atttctcgcg ggtatttaaa aaatgcaccg gggccagccc
    13801 gagcgagttc cgtgccggtt gtgaagaaaa agtgaatgat gtagccgtca agttgtcata
    13861 attggtaacg aatcagacaa ttgacggctt gacggagtag catagggttt gcagaatccc
    13921 tgcttcgtcc atttgacagg cacattatgc atcgatgata agctgtcaaa catgagcaga
    13981 tcctctacgc cggacgcatc gtggcctgtg ggcggacaat aaagtcttaa actgaacaaa
    14041 atagatctaa actatgacaa taaagtctta aactagacag aatagttgta aactgaaatc
    14101 agtccagtta tgctgtgaaa aagcatactg gacttttgtt atggctaaag caaactcttc
    14161 attttctgaa gtgcaaattg cccgtcgtat taaagagggg cgtggggtcg acgcggccgt
    14221 ggcgcgcctc ctaggtgctc gagtggcggc cgctattgac ccataaactg ccaggaattg
    14281 gggatcggat tcgagctcgc acgctgccgc aagcactcag ggcgcaaggg ctgctaaagg
    14341 aagcggaaca cgtagaaagc cagtccgcag aaacggtgct gaccccggat gaatgtcagc
    14401 tactgggcta tctggacaag ggaaaacgca agcgcaaaga gaaagcaggt agcttgcagt
    14461 gggcttacat ggcgatagct agactgggcg gttttatgga cagcaagcga accggaattg
    14521 ccagctgggg cgccctctgg taaggttggg aagccctgca aagtaaactg gatggctttc
    14581 ttgccgccaa ggatctgatg gcgcagggga tcaagatctg atcaagagac aggatactag
    14641 tggaggaaga aaaa
//
