LOCUS       sequence-226948-       14617 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  miniTn7 plasmid to deliver constitutively expressed fluorescent 
            protein genes in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14617)
  AUTHORS   Schlechter RO, Jun H, Bernach M, Oso S, Boyd E, Munoz-Lintz DA, 
            Dobson RCJ, Remus DM, Remus-Emsermann MNP
  TITLE     Chromatic Bacteria - A Broad Host-Range Plasmid and Chromosomal 
            Insertion Toolbox for Fluorescent Protein Expression in Bacteria.
  JOURNAL   Front Microbiol. 2018 Dec 12;9:3052. doi: 10.3389/fmicb.2018.03052. 
            eCollection 2018.
  PUBMED    30631309
REFERENCE   2  (bases 1 to 14617)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol."; date: "2018-12-12"; volume: "9:3052. doi"; pages: " 
            10.3389/fmicb.2018.03052. eCollection 2018"
COMMENT     Sequence Label: pMRE-Tn7-132
FEATURES             Location/Qualifiers
     source          1..14617
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(1..3,4..516)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIE"
     primer_bind     complement(43..64)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(304..323)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     651..672
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      701..795
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             839..1498
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(905..924)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1563..1649
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(2078..2956)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        2983..3267
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     3190..3209
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     primer_bind     complement(9958..9975)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      10128..10214
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      10306..10333
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        10352..10443
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10444..10512,10513..11304)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10662..10681)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     complement(11433..11452)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     oriT            11475..11584
                     /note="incP origin of transfer"
     primer_bind     complement(11622..11641)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     rep_origin      12324..12546
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             12594..13544
                     /codon_start=1
                     /gene="rep101 encoding an A56V mutation"
                     /product="temperature-sensitive version of the RepA protein
                     needed for replication with the pSC101 origin (Armstrong et
                     al., 1984)"
                     /label=Rep101(Ts)
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYVQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     mobile_element  13977..14142
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 atgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
       61 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
      121 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
      181 gtgaccaccc tgacctacgg cgtgcagtgc ttcgcccgct accccgacca catgaagcag
      241 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
      301 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
      361 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
      421 ctggagtaca actacaacag ccacaacgtc tatatcaccg ccgacaagca gaagaacggc
      481 atcaaggcca acttcaagat ccgccacaac atcgaggacg gcggcgtgca gctcgccgac
      541 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
      601 ctgagcaccc agtccaagct gagcaaagac cccaacgaga agcgcgatca catggtcctg
      661 ctggagttcg tgaccgccgc cgggtaatta gctgagcttg gactcctgtt gatagatcca
      721 gtaatgacct cagaactcca tctggatttg ttcagaacgc tcggttgccg ccgggcgttt
      781 tttattggtg agaatccaag ctagcttggc gagattttca ggagctaagg aagctaaaat
      841 ggagaaaaaa atcactggat ataccaccgt tgatatatcc caatggcatc gtaaagaaca
      901 ttttgaggca tttcagtcag ttgctcaatg tacctataac cagaccgttc agctggatat
      961 tacggccttt ttaaagaccg taaagaaaaa taagcacaag ttttatccgg cctttattca
     1021 cattcttgcc cgcctgatga atgctcatcc ggaatttcgt atggcaatga aagacggtga
     1081 gctggtgata tgggatagtg ttcacccttg ttacaccgtt ttccatgagc aaactgaaac
     1141 gttttcatcg ctctggagtg aataccacga cgatttccgg cagtttctac acatatattc
     1201 gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt ttattgagaa
     1261 tatgtttttc gtctcagcca atccctgggt gagtttcacc agttttgatt taaacgtggc
     1321 caatatggac aacttcttcg cccccgtttt caccatgggc aaatattata cgcaaggcga
     1381 caaggtgctg atgccgctgg cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt
     1441 cggcagaatg cttaatgaat tacaacagta ctgcgatgag tggcagggcg gggcgtaatt
     1501 tttttaaggc agttattggt gcccttaaac gcctggggta atgactctct agcttgaggc
     1561 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
     1621 cggtgaacgc tctcctgagt aggacaaatc cgccgctagg agcttgcggc cgccaggggt
     1681 caatagcggc cgccactcga gcacctagga ggcgcgccac ggccgcgtcg accccacgcc
     1741 cctctttaat acgacgggca atttgcactt cagaaaatga agagtttgct ttagccataa
     1801 caaaagtcca gtatgctttt tcacagcata actggactga tttcagttta caactattct
     1861 gtctagttta agactttatt gtcatagttt agatctattt tgttcagttt aagactttat
     1921 tgtccgccca caggccacga tgcgtccggc gtagaggatc tgctcatgtt tgacagctta
     1981 tcatcgatgc ataatgtgcc tgtcaaatgg acgaagcagg gattctgcaa accctatgct
     2041 actccgtcaa gccgtcaatt gtctgattcg ttaccaatta tgacaacttg acggctacat
     2101 cattcacttt ttcttcacaa ccggcacgga actcgctcgg gctggccccg gtgcattttt
     2161 taaatacccg cgagaaatag agttgatcgt caaaaccaac attgcgaccg acggtggcga
     2221 taggcatccg ggtggtgctc aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc
     2281 agcttaagac gctaatccct aactgctggc ggaaaagatg tgacagacgc gacggcgaca
     2341 agcaaacatg ctgtgcgacg ctggcgatat caaaattgct gtctgccagg tgatcgctga
     2401 tgtactgaca agcctcgcgt acccgattat ccatcggtgg atggagcgac tcgttaatcg
     2461 cttccatgcg ccgcagtaac aattgctcaa gcagatttat cgccagcagc tccgaatagc
     2521 gcccttcccc ttgcccggcg ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt
     2581 gcgcttcatc cgggcgaaag aaccccgtat tggcaaatat tgacggccag ttaagccatt
     2641 catgccagta ggcgcgcgga cgaaagtaaa cccactggtg ataccattcg cgagcctccg
     2701 gatgacgacc gtagtgatga atctctcctg gcgggaacag caaaatatca cccggtcggc
     2761 aaacaaattc tcgtccctga tttttcacca ccccctgacc gcgaatggtg agattgagaa
     2821 tataaccttt cattcccagc ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa
     2881 tcggcgttaa acccgccacc agatgggcat taaacgagta tcccggcagc aggggatcat
     2941 tttgcgcttc agccatactt ttcatactcc cgccattcag agaagaaacc aattgtccat
     3001 attgcatcag acattgccgt cactgcgtct tttactggct cttctcgcta accaaaccgg
     3061 taaccccgct tattaaaagc attctgtaac aaagcgggac caaagccatg acaaaaacgc
     3121 gtaacaaaag tgtctataat cacggcagaa aagtccacat tgattatttg cacggcgtca
     3181 cactttgcta tgccatagca tttttatcca taagattagc ggatcctacc tgacgctttt
     3241 tatcgcaact ctctactgtt tctccatacc cgtttttttg ggctagcgaa ttcgagctcg
     3301 gtacccaagt cttaaactag acagaatagt tgtaaactga aatcagtcca gttatgctgt
     3361 gaaaaagcat actggacttt tgttatggct aaagcaaact cttcattttc tgaagtgcaa
     3421 attgcccgtc gtattaaaga ggggcgtggc caagggcatg gtaaagacta tattccatgg
     3481 ctaacagtac aagaagttcc ttcttcaggt cgttcccacc gtatttattc tcataagacg
     3541 ggacgagtcc atcatttgct atctgactta gagcttgctg tttttctcag tcttgagtgg
     3601 gagagcagcg tgctagatat acgcgagcag ttccccttat tacctagtga taccaggcag
     3661 attgcaatag atagtggtat taagcatcct gttattcgtg gtgtagatca ggttatgtct
     3721 actgattttt tagtggactg caaagatggt ccttttgagc agtttgctat tcaagtcaaa
     3781 cctgcagcag ccttacaaga cgagcgtacc ttagaaaaac tagaactaga gcgtcgctat
     3841 tggcagcaaa agcaaattcc ttggttcatt tttactgata aagaaataaa tcccgtagta
     3901 aaagaaaata ttgaatggct ttattcagtg aaaacagaag aagtttctgc ggagctttta
     3961 gcacaactat ccccattggc ccatatcctg caagaaaaag gagatgaaaa cattatcaat
     4021 gtctgtaagc aggttgatat tgcttatgat ttggagttag gcaaaacatt gagtgagata
     4081 cgagccttaa ccgcaaatgg ttttattaag ttcaatattt ataagtcttt cagggcaaat
     4141 aagtgtgcag atctctgtat tagccaagta gtgaatatgg aggagttgcg ctatgtggca
     4201 aattaatgag gttgtgctat ttgataatga tccgtatcgc attttggcta tagaggatgg
     4261 ccaagttgtc tggatgcaaa taagcgctga taaaggagtt ccacaagcta gggctgagtt
     4321 gttgctaatg cagtatttag atgaaggccg cttagttaga actgatgacc cttatgtaca
     4381 tcttgattta gaagagccgt ctgtagattc tgtcagcttc cagaagcgcg aggaggatta
     4441 tcgaaaaatt cttcctatta ttaatagtaa ggatcgtttc gaccctaaag tcagaagcga
     4501 actcgttgag catgtggtcc aagaacataa ggttactaag gctacagttt ataagttgtt
     4561 acgccgttac tggcagcgtg gtcaaacgcc taatgcatta attcctgact acaaaaacag
     4621 cggtgcacca ggggaaagac gttcagcgac aggaacagca aagattggcc gagccagaga
     4681 atatggtaag ggtgaaggaa ccaaggtaac gcccgagatt gaacgccttt ttaggttgac
     4741 catagaaaag cacctgttaa atcaaaaagg tacaaagacc accgttgcct atagacgatt
     4801 tgtggacttg tttgctcagt attttcctcg cattccccaa gaggattacc caacactacg
     4861 tcagtttcgt tatttttatg atcgagaata ccctaaagct cagcgcttaa agtctagagt
     4921 taaagcaggg gtatataaaa aagacgtacg acccttaagt agtacagcca cttctcaggc
     4981 gttaggccct gggagtcgtt atgagattga tgccacgatt gctgatattt atttagtgga
     5041 tcatcatgat cgccaaaaaa tcataggaag accaacgctt tacattgtga ttgatgtgtt
     5101 tagtcggatg atcacgggct tttatatcgg ctttgaaaat ccgtcttatg tggtggcgat
     5161 gcaggctttt gtaaatgctt gctctgacaa aacggccatt tgtgcccagc atgatattga
     5221 gattagtagc tcagactggc cgtgtgtagg tttgccagat gtgttgctag cggaccgtgg
     5281 cgaattaatg agtcatcagg tcgaagcctt agtttctagt tttaatgtgc gagtggaaag
     5341 tgctccacct agacgtggcg atgctaaagg catagtggaa agcactttta gaacactaca
     5401 agccgagttt aagtcctttg cacctggcat tgtagagggc agtcggatca aaagccatgg
     5461 tgaaacagac tataggttag atgcatctct gtcggtattt gagttcacac aaattatttt
     5521 gcgtacgatc ttattcagaa ataaccatct ggtgatggat aaatacgatc gagatgctga
     5581 ttttcctaca gatttaccgt ctattcctgt ccagctatgg caatggggta tgcagcatcg
     5641 tacaggtagt ttaagggctg tggagcaaga gcagttgcga gtagcgttac tgcctcgccg
     5701 aaaggtctct atttcttcat ttggcgttaa tttgtggggt ttgtattact cggggtcaga
     5761 gattctgcgt gagggttggt tgcagcggag cactgatata gctagacctc aacatttaga
     5821 agcggcttat gacccagtgc tggttgatac gatttatttg tttccgcaag ttggcagccg
     5881 tgtattttgg cgctgtaatc tgacggaacg tagtcggcag tttaaaggtc tctcattttg
     5941 ggaggtttgg gatatacaag cacaagaaaa acacaataaa gccaatgcga agcaggatga
     6001 gttaactaaa cgcagggagc ttgaggcgtt tattcagcaa accattcaga aagcgaataa
     6061 gttaacgccc agtactactg agcccaaatc aacacgcatt aagcagatta aaactaataa
     6121 aaaagaagcc gtgacctcgg agcgtaaaaa acgtgcggag catttgaagc caagctcttc
     6181 aggtgatgag gctaaagtta ttcctttcaa cgcagtggaa gcggatgatc aagaagatta
     6241 cagcctaccc acatacgtgc ctgaattatt tcaggatcca ccagaaaagg atgagtcatg
     6301 agtgctaccc ggattcaagc agtttatcgt gatacggggg tagaggctta tcgtgataat
     6361 ccttttatcg aggccttacc accattacaa gagtcagtga atagtgctgc atcactgaaa
     6421 tcctctttac agcttacttc ctctgacttg caaaagtccc gtgttatcag agctcatacc
     6481 atttgtcgta ttccagatga ctattttcag ccattaggta cgcatttgct actaagtgag
     6541 cgtatttcgg tcatgattcg aggtggctac gtaggcagaa atcctaaaac aggagattta
     6601 caaaagcatt tacaaaatgg ttatgagcgt gttcaaacgg gagagttgga gacatttcgc
     6661 tttgaggagg cacgatctac ggcacaaagc ttattgttaa ttggttgttc tggtagtggg
     6721 aagacgacct ctcttcatcg tattctagcc acgtatcctc aggtgattta ccatcgtgaa
     6781 ctcaatgtag agcaggtggt gtatttgaaa atagactgct cgcataatgg ttcgctaaaa
     6841 gaaatctgct tgaatttttt cagagcgttg gatcgagcct tgggctcgaa ctatgagcgt
     6901 cgttatggct taaaacgtca tggtatagaa accatgttgg ctttgatgtc gcaaatagcc
     6961 aatgcacatg ctttagggtt gttggttatt gatgaaattc agcatttaag ccgctctcgt
     7021 tcgggtggat ctcaagagat gctgaacttt tttgtgacga tggtgaatat tattggcgta
     7081 ccagtgatgt tgattggtac ccctaaagca cgagagattt ttgaggctga tttgcggtct
     7141 gcacgtagag gggcagggtt tggagctata ttctgggatc ctatacaaca aacgcaacgt
     7201 ggaaagccca atcaagagtg gatcgctttt acggataatc tttggcaatt acagctttta
     7261 caacgcaaag atgcgctgtt atcggatgag gtccgtgatg tgtggtatga gctaagccaa
     7321 ggagtgatgg acattgtagt aaaacttttt gtactcgctc agctccgtgc gctagcttta
     7381 ggcaatgagc gtattaccgc tggtttattg cggcaagtgt atcaagatga gttaaagcct
     7441 gtgcacccca tgctagaggc attacgctcg ggtatcccag aacgcattgc tcgttattct
     7501 gatctagtcg ttcccgagat tgataaacgg ttaatccaac ttcagctaga tatcgcagcg
     7561 atacaagaac aaacaccaga agaaaaagcc cttcaagagt tagataccga agatcagcgt
     7621 catttatatc tgatgctgaa agaggattac gattcaagcc tgttaattcc cactattaaa
     7681 aaagcgttta gccagaatcc aacgatgaca agacaaaagt tactgcctct tgttttgcag
     7741 tggttgatgg aaggcgaaac ggtagtgtca gaactagaaa agccctccaa gagtaaaaag
     7801 gtttcggcta taaaggtagt caagcccagc gactgggata gcttgcctga tacggattta
     7861 cgttatatct attcacaacg ccaacctgaa aaaaccatgc atgaacggtt aaaagggaaa
     7921 ggggtaatag tggatatggc gagcttattt aaacaagcag gttagccatg agaaactttc
     7981 ctgttccgta ctcgaatgag ctgatttata gcactattgc acgggcaggc gtttatcaag
     8041 ggattgttag tcctaagcag ctgttggatg aggtgtatgg caaccgcaag gtggtcgcta
     8101 ccttaggtct gccctcgcat ttaggtgtga tagcaagaca tctacatcaa acaggacgtt
     8161 acgctgttca gcagcttatt tatgagcata ccttattccc tttatatgct ccgtttgtag
     8221 gcaaggagcg ccgagacgaa gctattcggt taatggagta ccaagcgcaa ggtgcggtgc
     8281 atttaatgct aggagtcgct gcttctagag ttaagagcga taaccgcttt agatactgcc
     8341 ctgattgcgt tgctcttcag ctaaataggt atggggaagc cttttggcaa cgagattggt
     8401 atttgcccgc tttgccatat tgtccaaaac acggtgcttt agtcttcttt gatagagctg
     8461 tagatgatca ccgacatcaa ttttgggctt tgggtcatac tgagctgctt tcagactacc
     8521 ccaaagactc cctatctcaa ttaacagcac tagctgctta tatagcccct ctgttagatg
     8581 ctccacgagc gcaagagctt tccccaagcc ttgagcagtg gacgctgttt tatcagcgct
     8641 tagcgcagga tctagggcta accaaaagca agcacattcg tcatgacttg gtggcggaga
     8701 gagtgaggca gacttttagt gatgaggcac tagagaaact ggatttaaag ttggcagaga
     8761 acaaggacac gtgttggctg aaaagtatat tccgtaagca tagaaaagcc tttagttatt
     8821 tacagcatag tattgtgtgg caagccttat tgccaaaact aacggttata gaagcgctac
     8881 agcaggcaag tgctcttact gagcactcta taacgacaag acctgttagc cagtctgtgc
     8941 aacctaactc tgaagattta tctgttaagc ataaagactg gcagcaacta gtgcataaat
     9001 accaaggaat taaggcggca agacagtctt tagagggtgg ggtgctatac gcttggcttt
     9061 accgacatga cagggattgg ctagttcact ggaatcaaca gcatcaacaa gagcgtctgg
     9121 cacccgcccc tagagttgat tggaaccaaa gagatcgaat tgctgtacga caactattaa
     9181 gaatcataaa gcgtctagat agtagccttg atcacccaag agcgacatcg agctggctgt
     9241 taaagcaaac tcctaacgga acctctcttg caaaaaatct acagaaactg cctttggtag
     9301 cgctttgctt aaagcgttac tcagagagtg tggaagatta tcaaattaga cggattagcc
     9361 aagcttttat taagcttaaa caggaagatg ttgagcttag gcgctggcga ttattaagaa
     9421 gtgcaacgtt atctaaagag cggataactg aggaagcaca aagattcttg gaaatggttt
     9481 atggggaaga gtgagtggtt aggctagcta catttaatga caatgtgcag gttgtacata
     9541 ttggtcattt attccgtaac tcgggtcata aggagtggcg tatttttgtt tggtttaatc
     9601 caatgcaaga acggaaatgg actcgattta ctcatttgcc tttattaagt cgagctaagg
     9661 tggttaacag tacaacaaag caaataaata aggcggatcg tgtgattgag tttgaagcat
     9721 cggatcttca acgagccaaa ataatcgatt ttcctaatct ctcgtccttt gcttccgtac
     9781 gcaacaagga tggagcgcag agttcattta tttacgaagc tgaaacacca tatagcaaga
     9841 ctcgttatca catcccacag ttagagctag ctcggtcatt atttttaggg gatcctctag
     9901 agtcgacctg caggcatgca agcttggctg ttttggcgga tgagagaaga ttttcagcct
     9961 gatacagatt aaatcagaac gcagaagcgg tctgataaaa cagaatttgc ctggcggcag
    10021 tagcgcggtg gtcccacctg accccatgcc gaactcagaa gtgaaacgcc gtagcgccga
    10081 tggtagtgtg gggtctcccc atgcgagagt agggaactgc caggcatcaa ataaaacgaa
    10141 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc
    10201 tgagtaggac aaatccgccg ggagcggatt tgaacgttgc gaagcaacgg cccggagggt
    10261 ggcgggcagg acgcccgcca taaactgcca ggcatcaaat taagcagaag gccatcctga
    10321 cggatggcct ttttgcgttt ctacaaactc ttttgtttat ttttctaaat acattcaaat
    10381 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
    10441 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
    10501 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
    10561 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
    10621 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
    10681 tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
    10741 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
    10801 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
    10861 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
    10921 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
    10981 atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
    11041 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
    11101 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
    11161 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
    11221 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
    11281 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
    11341 gatttacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
    11401 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
    11461 cgccacgttc gccgccggcc agcctcgcag agcaggattc ccgttgagca ccgccaggtg
    11521 cgaataaggg acagtgaaga aggaacaccc gctcgcgggt gggcctactt cacctatcct
    11581 gcccggcggc atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac
    11641 cgatggggaa gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat
    11701 ggtggcaggc cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct
    11761 tgcggcggcg gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc
    11821 gcataaggga gagcgtcgat ccccgacagt aagacgggta agcctgttga tgataccgct
    11881 gccttactgg gtgcattagc cagtctgaat gacctgtcac gggataatcc gaagtggtca
    11941 gactggaaaa tcagagggca ggaactgctg aacagcaaaa agtcagatag caccacatag
    12001 cagacccgcc ataaaacgcc ctgagaagcc cgtgacgggc ttttcttgta ttatgggtag
    12061 tttccttgca tgaatccata aaaggcgcct gtagtgccat ttacccccat tcactgccag
    12121 agccgtgagc gcagcgaact gaatgtcacg aaaaagacag cgactcaggt gcctgatggt
    12181 cggagacaaa aggaatattc agcgatttgc ccgagcttgc gagggtgcta cttaagcctt
    12241 tagggtttta aggtctgttt tgtagaggag caaacagcgt ttgcgacatc cttttgtaat
    12301 actgcggaac tgactaaagt agtgagttat acacagggct gggatctatt ctttttatct
    12361 ttttttattc tttctttatt ctataaatta taaccacttg aatataaaca aaaaaaacac
    12421 acaaaggtct agcggaattt acagagggtc tagcagaatt tacaagtttt ccagcaaagg
    12481 tctagcagaa tttacagata cccacaactc aaaggaaaag gactagtaat tatcattgac
    12541 tagcccatct caattggtat agtgattaaa atcacctaga ccaattgaga tgtatgtctg
    12601 aattagttgt tttcaaagca aatgaactag cgattagtcg ctatgactta acggagcatg
    12661 aaaccaagct aattttatgc tgtgtggcac tactcaaccc cacgattgaa aaccctacaa
    12721 ggaaagaacg gacggtatcg ttcacttata accaatacgt tcagatgatg aacatcagta
    12781 gggaaaatgc ttatggtgta ttagctaaag caaccagaga gctgatgacg agaactgtgg
    12841 aaatcaggaa tcctttggtt aaaggctttg agattttcca gtggacaaac tatgccaagt
    12901 tctcaagcga aaaattagaa ttagttttta gtgaagagat attgccttat cttttccagt
    12961 taaaaaaatt cataaaatat aatctggaac atgttaagtc ttttgaaaac aaatactcta
    13021 tgaggattta tgagtggtta ttaaaagaac taacacaaaa gaaaactcac aaggcaaata
    13081 tagagattag ccttgatgaa tttaagttca tgttaatgct tgaaaataac taccatgagt
    13141 ttaaaaggct taaccaatgg gttttgaaac caataagtaa agatttaaac acttacagca
    13201 atatgaaatt ggtggttgat aagcgaggcc gcccgactga tacgttgatt ttccaagttg
    13261 aactagatag acaaatggat ctcgtaaccg aacttgagaa caaccagata aaaatgaatg
    13321 gtgacaaaat accaacaacc attacatcag attcctacct acataacgga ctaagaaaaa
    13381 cactacacga tgctttaact gcaaaaattc agctcaccag ttttgaggca aaatttttga
    13441 gtgacatgca aagtaagtat gatctcaatg gttcgttctc atggctcacg caaaaacaac
    13501 gaaccacact agagaacata ctggctaaat acggaaggat ctgaggttct tatggctctt
    13561 gtatctatca gtgaagcatc aagactaaca aacaaaagta gaacaactgt tcaccgttac
    13621 atatcaaagg gaaaactgtc catatgcaca gatgaaaacg gtgtaaaaaa gatagataca
    13681 tcagagcttt tacgagtttt tggtgcattt aaagctgttc accatgaaca gatcgacaat
    13741 gtaacagatg aacagcatgt aacacctaat agaacaggtg aaaccagtaa aacaaagcaa
    13801 ctagaacatg aaattgaaca cctgagacaa cttgttacag ctcaacagtc acacatagac
    13861 agcctgaaac aggcgatgct gcttatcgaa tcaaagctgc cgacaacacg ggagccagtg
    13921 acgcctcccg tggggaaaaa atcatggcaa ttctggaaga aatagcgctt tcagcctgtg
    13981 ggcggacaaa atagttggga actgggaggg gtggaaatgg agtttttaag gattatttag
    14041 ggaagagtga caaaatagat gggaactggg tgtagcgtcg taagctaata cgaaaattaa
    14101 aaatgacaaa atagtttgga actagatttc acttatctgg ttggtcgaca ctagtattac
    14161 cctgttatcc ctagatttaa atgatatcgg atcctagtaa gccacgtttt aattaatcag
    14221 atcccataaa ctgccaggaa ttggggatcg gattcgagct cgcacgctgc cgcaagcact
    14281 cagggcgcaa gggctgctaa aggaagcgga acacgtagaa agccagtccg cagaaacggt
    14341 gctgaccccg gatgaatgtc agctactggg ctatctggac aagggaaaac gcaagcgcaa
    14401 agagaaagca ggtagcttgc agtgggctta catggcgata gctagactgg gcggttttat
    14461 ggacagcaag cgaaccggaa ttgccagctg gggcgccctc tggtaaggtt gggaagccct
    14521 gcaaagtaaa ctggatggct ttcttgccgc caaggatctg atggcgcagg ggatcaagat
    14581 ctgatcaaga gacaggatac tagtggagga agaaaaa
//
