LOCUS       sequence-22695-e         737 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Novitch BG, Wichterle H, Jessell TM, Sockanathan S
  TITLE     A requirement for retinoic acid-mediated transcriptional activation 
            in ventral neural patterning and motor neuron specification.
  JOURNAL   Neuron. 2003 Sep 25. 40(1):81-95.
  PUBMED    14527435
REFERENCE   2  (bases 1 to 737)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2003 Sep 25. 40(1):81-95."
COMMENT     Sequence Label: pCIG-VP16 RARa [TJ#389]
FEATURES             Location/Qualifiers
     source          1..737
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     2..18
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     3..19
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             37..270
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
ORIGIN
        1 ctcgaggtcg acggtatcga taagcttgcc accatggccc ccccgaccga tgtcagcctg
       61 ggggacgagc tccacttaga cggcgaggac gtggcgatgg cgcatgccga cgcgctagac
      121 gatttcgatc tggacatgtt gggggacggg gattccccgg gtccgggatt taccccccac
      181 gactccgccc cctacggcgc tctggatatg gccgacttcg agtttgagca gatgtttacc
      241 gatgcccttg gaattgacga gtacggtggg aagcttctag gtaccagatc cagcaacagc
      301 agctcctgcc cgacacctgg gggcgggcac ctcaatgggt acccggtgcc tccctacgcc
      361 ttcttcttcc cccctatgct gggtggactc tccccgccag gcgctctgac cactctccag
      421 caccagcttc cagttagtgg atatagcaca ccatccccag ccaccattga gacccagagc
      481 agcagttctg aagagatagt gcccagccct ccctcgccac cccctctacc ccgcatctac
      541 aagccttgct ttgtctgtca ggacaagtcc tcaggctacc actatggggt cagcgcctgt
      601 gagggctgca agggcttctt ccgccgcagc atccagaaga acatggtgta cacgtgtcac
      661 cgggacaaga actgcatcat caacaaggtg acccggaacc gctgccagta ctgccgactg
      721 cagaagtgct ttgaagt
//
