LOCUS       sequence-226951-        5914 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N-terminus to aa326 of TP901 integrase fused to GID1 CID.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5914)
  AUTHORS   Weinberg BH, Cho JH, Agarwal Y, Pham NTH, Caraballo LD, Walkosz M, 
            Ortega C, Trexler M, Tague N, Law B, Benman WKJ, Letendre J, Beal J,
            Wong WW
  TITLE     High-performance chemical- and light-inducible recombinases in 
            mammalian cells and mice.
  JOURNAL   Nat Commun. 2019 Oct 24;10(1):4845. doi: 10.1038/s41467-019-12800-7.
  PUBMED    31649244
REFERENCE   2  (bases 1 to 5914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-10-24"; volume: "10(1):4845. doi"; pages: " 
            10.1038/s41467-019-12800-7"
COMMENT     Sequence Label: pSD35_pCAG-PV1-TP901-N326-L1-FRB-NLS-BGHpA
FEATURES             Location/Qualifiers
     source          1..5914
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     466..488
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     550..569
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1697..1972
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRIS"
     CDS             1973..1993
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2003..2227
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(2467..2486)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2532..2587
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2586..2605)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(2948..2964)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2948..2964)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2961..2983)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2972..2988
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2996..3002,3003..3020,3021..3026))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3041..3062
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      3147..3281
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3301..3435
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3338..3357)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3392..3411
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3503..3520)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3674..4262)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3754..3773)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4433..5224,5225..5293))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5056..5075
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5294..5398)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        5429..5808
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg
       61 ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg
      121 gggcggggcc gcctcgggcc ggggagggct cgggggaggg gcgcggcggc ccccggagcg
      181 ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga
      241 gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc
      301 gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg
      361 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct
      421 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc
      481 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac
      541 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattgatta
      601 attcgagcga aggcgcgccc attactcgca tccattctca ggctgtctcg tctcgtctcg
      661 cacgcgtgcc gccaccatga ccaagaaggt ggccatctac accagagtgt ccaccaccaa
      721 ccaggccgag gaaggcttca gcatcgacga gcagatcgac cggctgacca aatacgccga
      781 ggccatggga tggcaggtgt ccgataccta caccgacgcc ggctttagcg gcgccaagct
      841 ggaaagaccc gccatgcagc ggctgatcaa cgacatcgag aacaaggcct tcgacaccgt
      901 gctggtgtac aagctggaca ggctgagcag aagcgtgcgg gacaccctgt acctcgtgaa
      961 ggacgtgttc accaagaaca agatcgactt catcagcctg aacgagagca tcgacaccag
     1021 cagcgctatg ggcagcctgt tcctgaccat cctgagcgcc atcaacgagt tcgagcgcga
     1081 gaacatcaaa gaacggatga ccatgggcaa gctgggcaga gccaagagcg gcaagagcat
     1141 gatgtggacc aagaccgcct tcggctacta ccacaacaga aagaccggca tcctggaaat
     1201 agtgccactg caggccacca tcgtggaaca gatcttcacc gactacctga gcggcatctc
     1261 cctgaccaag ctgagagaca agctgaacga gtccggccac atcggcaagg acatcccttg
     1321 gagctaccgg accctgcggc agaccctgga caaccctgtg tactgcggct acatcaagtt
     1381 caaggactcc ctgttcgagg gcatgcacaa gcccatcatc ccttacgaga catacctgaa
     1441 ggtgcagaaa gagctggaag agagacagca gcagacctac gagcggaaca acaaccccag
     1501 acccttccag gccaagtaca tgctgtccgg catggccaga tgcggctact gtggcgcccc
     1561 tctgaagatc gtgctgggcc acaagagaaa ggacggcagc cggaccatga agtaccactg
     1621 cgccaaccgg ttccctagaa agaccaaggg catctccgga gggtctggct ccggatcaag
     1681 tggtggcagc ggtaccatcc tctggcatga gatgtggcat gaaggcctgg aagaggcatc
     1741 tcgtttgtac tttggggaaa ggaacgtgaa aggcatgttt gaggtgctgg agcccttgca
     1801 tgctatgatg gaacggggcc cccagactct gaaggaaaca tcctttaatc aggcctatgg
     1861 tcgagattta atggaggccc aagagtggtg caggaagtac atgaaatcag ggaatgtcaa
     1921 ggacctcctc caagcctggg acctctatta tcatgtgttc cgacgaatct cacccaagaa
     1981 aaagcggaag gtgtgagaat tcctgtgcct tctagttgcc agccatctgt tgtttgcccc
     2041 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     2101 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     2161 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     2221 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
     2281 tgtagcgtgt accgctggga gttcgtagac ggaaacaaac gcagaatcca agcgcggccg
     2341 cccaggatac atagattacc acaactccga gcccttccac cgctagcgca ctcctcaggt
     2401 gcaggctgcc tatcagaagg tggtggctgg tgtggccaat gccctggctc acaaatacca
     2461 ctgagatctt tttccctctg ccaaaaatta tggggacatc atgaagcccc ttgagcatct
     2521 gacttctggc taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg
     2581 tctctcactc ggaaggacat atgggagggc aaatcattta aaacatcaga atgagtattt
     2641 ggtttagagt ttggcaacat atgcccatat gctggctgcc atgaacaaag gttggctata
     2701 aagaggtcat cagtatatga aacagccccc tgctgtccat tccttattcc atagaaaagc
     2761 cttgacttga ggttagattt tttttatatt ttgttttgtg ttattttttt ctttaacatc
     2821 cctaaaattt tccttacatg ttttactagc cagatttttc ctcctctcct gactactccc
     2881 agtcatagct gtccctcttc tcttatggag atccctcgac ctgcagccca agcttggcgt
     2941 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     3001 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     3061 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagcggatcc
     3121 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccagtt ccgcccattc
     3181 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     3241 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     3301 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     3361 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     3421 tatcatgtct ggatccgctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     3481 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     3541 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     3601 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     3661 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     3721 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     3781 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     3841 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     3901 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     3961 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     4021 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     4081 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     4141 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     4201 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     4261 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     4321 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     4381 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     4441 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     4501 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     4561 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     4621 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     4681 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     4741 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     4801 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     4861 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     4921 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     4981 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     5041 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     5101 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     5161 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     5221 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     5281 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     5341 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     5401 catttccccg aaaagtgcca cctgggtcga cattgattat tgactagtta ttaatagtaa
     5461 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
     5521 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
     5581 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
     5641 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
     5701 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
     5761 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt cgaggtgagc
     5821 cccacgttct gcttcactct ccccatctcc cccccctccc cacccccaat tttgtattta
     5881 tttatttttt aattattttg tgcagcgatg gggg
//
