LOCUS       sequence-226972-       10407 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express Sec7-iGFPx6 at the endogenous locus via pop-in pop-out 
            mutagenesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10407)
  AUTHORS   Casler JC, Papanikou E, Barrero JJ, Glick BS
  TITLE     Maturation-driven transport and AP-1-dependent recycling of a 
            secretory cargo in the Golgi.
  JOURNAL   J Cell Biol. 2019 May 6;218(5):1582-1601. doi: 
            10.1083/jcb.201807195. Epub 2019 Mar 11.
  PUBMED    30858194
REFERENCE   2  (bases 1 to 10407)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201807195"; journalName: "J Cell Biol"; date: 
            "2019-05-6- 6"; volume: "218"; issue: "5"; pages: "1582-1601"
COMMENT     Sequence Label: pSec7-iGFPx6
FEATURES             Location/Qualifiers
     source          1..10407
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7065..7081)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7065..7081)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7078..7100)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7089..7105
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7113..7119,7120..7137,7138..7143))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7158..7179
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7296..7313)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7467..8055)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7547..7566)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8226..9017,9018..9086))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8849..8868
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9087..9191)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             complement(9321..10121)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQLN"
     promoter        complement(10122..10337)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     10380..10398
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt ctattgttgt caaatgtgtt
      421 ttacagcttt tgatgataga gctgctcaac gaattattcg agaacgaaga ttttgcccac
      481 tgtatccctt acaaggaagc cattagaatt acaagattgt tggagaaatc gtacgaattt
      541 tctcgtgact ttaatgaaga ttatgggtta agaacaagac tagtagaggc tcgtgtagtt
      601 gataaaatac caaacctgtt gaaacaagaa acgagtgctg ctgcagttct ccttgacatt
      661 atgtttcagt tgtatctgaa cgatgatgag aagaaggctg atttaataac tcgtctgatc
      721 accatttgca tccaggtcgt agagggttat gtttctttgg atgatagaac catggaacgt
      781 agtatcaatg cctggcgttc agtgatagtt gaaatattac aaggttacta cgaatttgat
      841 gatgaagact ttagactata ctgtcctgct atgtatgctc tggtgattca aatattagat
      901 aagagcgttc caacggagtt gagacatgct ataaaacaat ttctaagcag agttggtgaa
      961 ttataccttt ctacggatcc aggaagttcg gggtccagcg gttcttcagg cgctagcatg
     1021 gatagcactg agagcctttt cactggagtt gtcccaattc ttgttgaatt agatggtgat
     1081 gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa
     1141 cttaccctta aatttatttg cactactgga aaactacctg ttccttggcc aacacttgtc
     1201 actactttaa cttatggtgt tcagtgcttt tcaagatacc cggatcatat gaaacagcat
     1261 gactttttca agagtgccat gcccgaaggt tatgtacagg aaagaactat atttttcaaa
     1321 gatgacggga actacaagac acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat
     1381 agaatcgagt taaaaggtat tgattttaaa gaagatggaa acattcttgg gcacaaattg
     1441 gaatacaact ataactcaca caatgtatac atcatggcag acaaacaaaa gaatggaatc
     1501 aaagctaact tcaaaattag acacaacatt gaagatggaa gcgttcaact agcagaccat
     1561 tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg
     1621 tccacacaat ctgccctttc gaaagatccc aacgaaaaga gagaccacat ggtccttaaa
     1681 gagtttgtaa cagctgctgg gattacacat ggcagctccg gctccagcgg gtctagcatg
     1741 gatagcactg agagcctttt cactggagtt gtcccaattc ttgttgaatt agatggtgat
     1801 gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa
     1861 cttaccctta aatttatttg cactactgga aaactacctg ttccttggcc aacacttgtc
     1921 actactttaa cttatggtgt tcagtgcttt tcaagatacc cggatcatat gaaacagcat
     1981 gactttttca agagtgccat gcccgaaggt tatgtacagg aaagaactat atttttcaaa
     2041 gatgacggga actacaagac acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat
     2101 agaatcgagt taaaaggtat tgattttaaa gaagatggaa acattcttgg gcacaaattg
     2161 gaatacaact ataactcaca caatgtatac atcatggcag acaaacaaaa gaatggaatc
     2221 aaagctaact tcaaaattag acacaacatt gaagatggaa gcgttcaact agcagaccat
     2281 tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg
     2341 tccacacaat ctgccctttc gaaagatccc aacgaaaaga gagaccacat ggtccttaaa
     2401 gagtttgtaa cagctgctgg gattacacat ggcagctccg gctccagcgg gtctagcatg
     2461 gatagcactg agagcctttt cactggagtt gtcccaattc ttgttgaatt agatggtgat
     2521 gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa
     2581 cttaccctta aatttatttg cactactgga aaactacctg ttccttggcc aacacttgtc
     2641 actactttaa cttatggtgt tcagtgcttt tcaagatacc cggatcatat gaaacagcat
     2701 gactttttca agagtgccat gcccgaaggt tatgtacagg aaagaactat atttttcaaa
     2761 gatgacggga actacaagac acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat
     2821 agaatcgagt taaaaggtat tgattttaaa gaagatggaa acattcttgg gcacaaattg
     2881 gaatacaact ataactcaca caatgtatac atcatggcag acaaacaaaa gaatggaatc
     2941 aaagctaact tcaaaattag acacaacatt gaagatggaa gcgttcaact agcagaccat
     3001 tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg
     3061 tccacacaat ctgccctttc gaaagatccc aacgaaaaga gagaccacat ggtccttaaa
     3121 gagtttgtaa cagctgctgg gattacacat ggcagctccg gctccagcgg gtctagcatg
     3181 gatagcactg agagcctttt cactggagtt gtcccaattc ttgttgaatt agatggtgat
     3241 gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa
     3301 cttaccctta aatttatttg cactactgga aaactacctg ttccttggcc aacacttgtc
     3361 actactttaa cttatggtgt tcagtgcttt tcaagatacc cggatcatat gaaacagcat
     3421 gactttttca agagtgccat gcccgaaggt tatgtacagg aaagaactat atttttcaaa
     3481 gatgacggga actacaagac acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat
     3541 agaatcgagt taaaaggtat tgattttaaa gaagatggaa acattcttgg gcacaaattg
     3601 gaatacaact ataactcaca caatgtatac atcatggcag acaaacaaaa gaatggaatc
     3661 aaagctaact tcaaaattag acacaacatt gaagatggaa gcgttcaact agcagaccat
     3721 tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg
     3781 tccacacaat ctgccctttc gaaagatccc aacgaaaaga gagaccacat ggtccttaaa
     3841 gagtttgtaa cagctgctgg gattacacat ggcagctccg gctccagcgg gtctagcatg
     3901 gatagcactg agagcctttt cactggagtt gtcccaattc ttgttgaatt agatggtgat
     3961 gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa
     4021 cttaccctta aatttatttg cactactgga aaactacctg ttccttggcc aacacttgtc
     4081 actactttaa cttatggtgt tcagtgcttt tcaagatacc cggatcatat gaaacagcat
     4141 gactttttca agagtgccat gcccgaaggt tatgtacagg aaagaactat atttttcaaa
     4201 gatgacggga actacaagac acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat
     4261 agaatcgagt taaaaggtat tgattttaaa gaagatggaa acattcttgg gcacaaattg
     4321 gaatacaact ataactcaca caatgtatac atcatggcag acaaacaaaa gaatggaatc
     4381 aaagctaact tcaaaattag acacaacatt gaagatggaa gcgttcaact agcagaccat
     4441 tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg
     4501 tccacacaat ctgccctttc gaaagatccc aacgaaaaga gagaccacat ggtccttaaa
     4561 gagtttgtaa cagctgctgg gattacacat ggcagctccg gctccagcgg gtctagcatg
     4621 gatagcactg agagcctttt cactggagtt gtcccaattc ttgttgaatt agatggtgat
     4681 gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa
     4741 cttaccctta aatttatttg cactactgga aaactacctg ttccttggcc aacacttgtc
     4801 actactttaa cttatggtgt tcagtgcttt tcaagatacc cggatcatat gaaacagcat
     4861 gactttttca agagtgccat gcccgaaggt tatgtacagg aaagaactat atttttcaaa
     4921 gatgacggga actacaagac acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat
     4981 agaatcgagt taaaaggtat tgattttaaa gaagatggaa acattcttgg gcacaaattg
     5041 gaatacaact ataactcaca caatgtatac atcatggcag acaaacaaaa gaatggaatc
     5101 aaagctaact tcaaaattag acacaacatt gaagatggaa gcgttcaact agcagaccat
     5161 tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg
     5221 tccacacaat ctgccctttc gaaagatccc aacgaaaaga gagaccacat ggtccttaaa
     5281 gagtttgtaa cagctgctgg gattacacat ggcagctccg gctccagcgg gtaaagcggc
     5341 cgggctttgt tgaatggtcc agcagattaa aatatgctta gttgtagaac acagaaaaaa
     5401 tgagaaggtt gatatacttt tactcatatt taccctgatt taatggcggt gatccttttt
     5461 agattcgttt aattcataaa caaggtacat catatagcaa taacaaatat gtaaactatg
     5521 tagaaagact tatcaagttt agccatcttg gtcctcattc atgggcattt tatactaact
     5581 tcatttttgc cgaaataaga aaggatgtaa tcttaaccta aaatatgtaa gcaattaagg
     5641 gtgcacgtag ttacttgcga acaaatagga aaagaaagaa acaagcacgg atactgaatg
     5701 actgagtgta ttactacacg atttatctca acgaatttaa gtgtggagga cgatcggctg
     5761 cacgtaggag tcaacgtttt tttactgtgt tcatcacaaa accattaagc tgagtcatcc
     5821 atattgcaaa caataaggta gcaccgcttc catcaaaaca ctagaacata ccgatgtttt
     5881 cacggattta ttggaaacaa atagcagatg agggcgtttt tacttgtaat gaaggcgaat
     5941 gagaaggcaa ggcttacagg cttgttgttt atgccaagca tcaatactcg ctatggacga
     6001 gcgccatcag cgataacaac aaagtttgca agtgcttgct tggatataaa aggcttgacg
     6061 cttaagataa atagggccat acttgcttgc ttttactatt tttgttcctc ataagcctga
     6121 ttgactacgc tattataaac ccctacatta tcctataatc aacttcactt gtgtaacgtt
     6181 gaatgagttc aactgagtaa tactcggtcg gaaatatacg ctccatctgg caggaaacat
     6241 aggctgcatt tatatcttgc acaattatgc ccttcgtagg tgtatgtttt catgttttcc
     6301 attatgtcaa tgtagatgta tctttggaca ggcaactttt ggatcaataa agcacaattt
     6361 ttgagcaatt tgagatatcc agtatttgta cgggagtccc aagattgttt agcctaaaag
     6421 gttgaggact tggattattc ttccatcaag cgcctccacc cctcgcatcc tatttctttt
     6481 gaagcatagg ggacgagact gtctgtaatt tatggcgaag tatgacaaga aagattcaaa
     6541 ctttgtttta cgtatgcccc aataaattct atggtagcac tactagagac gtattttggt
     6601 tttgtaataa ttttcaaact gctcgctatg atttgttaca agaaacaaac aaaagacacg
     6661 accatagggt tcatgttggt ggttgcgaag ccacatcttt aaaacattac tgcaaattca
     6721 gcaggagtga gtagcaaaat cgacaaaaac tagagtacgc aacattcccg gtcgtaaatt
     6781 tcttatcgtg gcgccagaac attcccgtcc aatgatgaga atgtctctaa aacaaatgca
     6841 gtcacagaga ttttctgcga cttctcgagt gagaatgttg cttattggtg ctagtggctt
     6901 gagatcttcg tcaagcaaat tggaatgccc ccagattctc aaacaagcac ggcagaaact
     6961 gaaatacggc caaaattatg aacccttttg tgggatcttt gtagaaatag gacctcagga
     7021 tagtatgatg aagaatgttg gcatgcaagc ttggcgtaat catggtcata gctgtttcct
     7081 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     7141 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     7201 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     7261 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     7321 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     7381 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     7441 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     7501 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     7561 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     7621 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     7681 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     7741 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     7801 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     7861 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     7921 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     7981 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     8041 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     8101 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     8161 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     8221 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     8281 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     8341 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     8401 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     8461 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     8521 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     8581 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     8641 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     8701 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     8761 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     8821 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     8881 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     8941 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     9001 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     9061 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     9121 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     9181 ggggttccgc gcacatttcc ccgaaaagtg ccacctagct ttttctttcc aatttttttt
     9241 ttttcgtcat tataaaaatc attacgaccg agattcccgg gtaataactg atataattaa
     9301 attgaagctc taatttgtga gtttagctgg ccgcatcttc tcaaatatgc ttcccagcct
     9361 gcttttctgt aacgttcacc ctctacctta gcatcccttc cctttgcaaa tagtcctctt
     9421 ccaacaataa taatgtcaga tcctgtagag accacatcat ccacggttct atactgttga
     9481 cccaatgcgt ctcccttgtc atctaaaccc acaccgggtg tcataatcaa ccaatcgtaa
     9541 ccttcatctc ttccacccat gtctctttga gcaataaagc cgataacaaa atctttgtcg
     9601 ctcttcgcaa tgtcaacagt acccttagta tattctccag tagataggga gcccttgcat
     9661 gacaattctg ctaacatcaa aaggcctcta ggttcctttg ttacttcttc tgccgcctgc
     9721 ttcaaaccgc taacaatacc tgggcccacc acaccgtgtg cattcgtaat gtctgcccat
     9781 tctgctattc tgtatacacc cgcagagtac tgcaatttga ctgtattacc aatgtcagca
     9841 aattttctgt cttcgaagag taaaaaattg tacttggcgg ataatgcctt tagcggctta
     9901 actgtgccct ccatggaaaa atcagtcaag atatccacat gtgtttttag taaacaaatt
     9961 ttgggaccta atgcttcaac taactccagt aattccttgg tggtacgaac atccaatgaa
    10021 gcacacaagt ttgtttgctt ttcgtgcatg atattaaata gcttggcagc aacaggacta
    10081 ggatgagtag cagcacgttc cttatatgta gctttcgaca tgatttatct tcgtttcctg
    10141 caggtttttg ttctgtgcag ttgggttaag aatactgggc aatttcatgt ttcttcaaca
    10201 ctacatatgc gtatatatac caatctaagt ctgtgctcct tccttcgttc ttccttctgt
    10261 tcggagatta ccgaatcaaa aaaatttcaa ggaaaccgaa atcaaaaaaa agaataaaaa
    10321 aaaaatgatg aattgaaaag ctctaagaaa ccattattat catgacatta acctataaaa
    10381 ataggcgtat cacgaggccc tttcgtc
//
