LOCUS       sequence-226973-        9076 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses FsRT-Cas1-Cas2 under pT7lac and sfGFP under ptetA 
            promoter, encodes FsCRISPR array 2, compatible with SENECA 
            acquisition readout.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9076)
  AUTHORS   Schmidt F, Cherepkova MY, Platt RJ
  TITLE     Transcriptional recording by CRISPR spacer acquisition from RNA.
  JOURNAL   Nature. 2018 Oct;562(7727):380-385. doi: 10.1038/s41586-018-0569-1. 
            Epub 2018 Oct 3.
  PUBMED    30283135
REFERENCE   2  (bases 1 to 9076)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-018-0569-1"; journalName: "Nature"; date: "2018-10";
            volume: "562"; issue: "7727"; pages: "380-385"
COMMENT     Sequence Label: 
            pFS_0271_pET30_pCat-tetR-Term-ptetA-sfGFP-rrnBterm_Fusicatenibacter_
            saccharivorans_ARRAY2_FaqI
FEATURES             Location/Qualifiers
     source          1..9076
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(210..1025)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(210..1025)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     933..952
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1017..1036
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      1147..1735
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1636..1655
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1889..1906
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    1921..2060
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(1986..2005)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2146..2168
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2162..2353)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(3162..4244)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     4206..4225
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(4245..4322)
                     /gene="lacI"
                     /label=lacI promoter
     RBS             4476..4487
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             4494..5117
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(4567..4586)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     terminator      5162..5233
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5249..5276
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     protein_bind    5291..5309
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5316..5334
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             join(5373..5375,5376..6089)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             5373..6086
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(5403..5431)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     6026..6046
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      6111..6197
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     6315..6334
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     6422..6441
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6422..6440
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    6441..6465
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             6480..6502
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(8755..8773)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      8769..8816
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ggccgctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
       61 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag
      121 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
      181 caaatatgta tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac
      241 tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat
      301 gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg
      361 attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta
      421 tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc
      481 atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca
      541 tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg
      601 ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca
      661 tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg
      721 gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc
      781 ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg
      841 gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat
      901 cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa
      961 tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg
     1021 ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa
     1081 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     1141 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     1201 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     1261 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     1321 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     1381 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     1441 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     1501 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     1561 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     1621 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     1681 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     1741 cagcaacgcg gcctttttac ggttcctggt cttttgctgg ccttttgctc acatgttctt
     1801 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     1861 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     1921 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca atggtgcact
     1981 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac
     2041 gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     2101 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     2161 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag
     2221 cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt
     2281 tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt
     2341 cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac
     2401 cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac
     2461 tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca
     2521 ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc
     2581 agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca
     2641 gactttacga aacacggaaa ccgaagatca ttcatgttgt tgctcaggtc gcagacgttt
     2701 tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa
     2761 ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg
     2821 gggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag
     2881 tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca
     2941 tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct
     3001 gtcctacgag ttgcatgata aagaagatag tcataagtgc ggcgacgata gtcatgcccc
     3061 gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgagatcccg
     3121 gtgcctaatg agtgagctaa cttacattaa ttgcgttgcg ctcactgccc gctttccagt
     3181 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     3241 tgcgtattgg gcgccagggt ggtttttctt ttcaccagtg agacgggcaa cagctgattg
     3301 cccttcaccg cctggccctg agagagttgc agcaagcggt ccacgctggt ttgccccagc
     3361 aggcgaaaat cctgtttgat ggtggttaac ggcgggatat aacatgagct atcttcggta
     3421 tcgtcgtatc ccactaccga gatgtccgca ccaacgcgca gcccggactc ggtaatggcg
     3481 cgcattgcgc ccagcgccat ctgatcgttg gcaaccagca tcgcagtggg aacgatgccc
     3541 tcattcagca tttgcatggt ttgttgaaaa ccggacatgg cactccagtc gccttcccgt
     3601 tccgctatcg gctgaatttg attgcgagtg agatatttat gccagccagc cagacgcaga
     3661 cgcgccgaga cagaacttaa tgggcccgct aacagcgcga tttgctggtg acccaatgcg
     3721 accagatgct ccacgcccag tcgcgtacca tcttcatggg agaaaataat actgttgatg
     3781 ggtgtctggt cagagacatc aagaaataac gccggaacat tagtgcaggc agcttccaca
     3841 gcaatggcat cctggtcatc cagcggatag ttaatgatca gcccactgac gcgttgcgcg
     3901 agaagattgt gcaccgccgc tttacaggct tcgacgccgc ttcgttctac catcgacacc
     3961 accacgctgg cacccagttg atcggcgcga gatttaatcg ccgcgacaat ttgcgacggc
     4021 gcgtgcaggg ccagactgga ggtggcaacg ccaatcagca acgactgttt gcccgccagt
     4081 tgttgtgcca cgcggttggg aatgtaattc agctccgcca tcgccgcttc cactttttcc
     4141 cgcgttttcg cagaaacgtg gctggcctgg ttcaccacgc gggaaacggt ctgataagag
     4201 acaccggcat actctgcgac atcgtataac gttactggtt tcacattcac caccctgaat
     4261 tgactctctt ccgggcgcta tcatgccata ccgcgaaagg ttttgcgcca ttcgatggtg
     4321 tccgggatct cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag
     4381 gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaccggtg gcacgtaaga
     4441 ggttccaact ttcaccataa tgaaacatac tagagaaaga ggagaaatac tagatgtcca
     4501 gattagataa aagtaaagtg attaacagcg cattagagct gcttaatgag gtcggaatcg
     4561 aaggtttaac aacccgtaaa ctcgcccaga agctaggtgt agagcagcct acattgtatt
     4621 ggcatgtaaa aaataagcgg gctttgctcg acgccttagc cattgagatg ttagataggc
     4681 accatactca cttttgccct ttagaagggg aaagctggca agatttttta cgtaataacg
     4741 ctaaaagttt tagatgtgct ttactaagtc atcgcgatgg agcaaaagta catttaggta
     4801 cacggcctac agaaaaacag tatgaaactc tcgaaaatca attagccttt ttatgccaac
     4861 aaggtttttc actagagaat gcattatatg cactcagcgc tgtggggcat tttactttag
     4921 gttgcgtatt ggaagatcaa gagcatcaag tcgctaaaga agaaagggaa acacctacta
     4981 ctgatagtat gccgccatta ttacgacaag ctatcgaatt atttgatcac caaggtgcag
     5041 agccagcctt cttattcggc cttgaattga tcatatgcgg attagaaaaa caacttaaat
     5101 gtgaaagtgg gtcctaataa cactgatagt gctagtgtag atcactacta gagccaggca
     5161 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
     5221 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta
     5281 tatactagag tccctatcag tgatagagat tgacatccct atcagtgata gagatactga
     5341 gcactactag agaagcttgc ggaggagcat gcatgagcaa aggagaagaa cttttcactg
     5401 gagttgtccc aattcttgtt gaattagatg gtgatgttaa tgggcacaaa ttttctgtcc
     5461 gtggagaggg tgaaggtgat gctacaaacg gaaaactcac ccttaaattt atttgcacta
     5521 ctggaaaact acctgttccg tggccaacac ttgtcactac tctgacctat ggtgttcaat
     5581 gcttttcccg ttatccggat cacatgaaac ggcatgactt tttcaagagt gccatgcccg
     5641 aaggttatgt acaggaacgc actatatctt tcaaagatga cgggacctac aagacgcgtg
     5701 ctgaagtcaa gtttgaaggt gatacccttg ttaatcgtat cgagttaaag ggtattgatt
     5761 ttaaagaaga tggaaacatt cttggacaca aactcgagta caactttaac tcacacaatg
     5821 tatacatcac ggcagacaaa caaaagaatg gaatcaaagc taacttcaaa attcgccaca
     5881 acgttgaaga tggttccgtt caactagcag accattatca acaaaatact ccaattggcg
     5941 atggccctgt ccttttacca gacaaccatt acctgtcgac acaatctgtc ctttcgaaag
     6001 atcccaacga aaagcgtgac cacatggtcc ttcttgagtt tgtaactgct gctgggatta
     6061 cacatggcat ggatgaactc tacaaatgat aggatcggat ctccaggcat caaataaaac
     6121 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
     6181 tcctgagtag gacaaatccg gatcccatgc aaggagatgg cgcccaacag tcccccggcc
     6241 acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc
     6301 cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg
     6361 gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tcgatctcga tcccgcgaaa
     6421 ttaatacgac tcactatagg ggaattgtga gcggataaca attcccctct agaaataatt
     6481 ttgtttaact ttaagaagga gatatacata tgttcactat agacgagatg ctatcaaaga
     6541 acaaccaaag attggctttc gagcacttcg ctactaagaa cgacggatgt ggccctgatg
     6601 gtatgcacgt atctgagctg gagaaatatt ggcgtatgaa tcacgatcag atcatctccg
     6661 acttgaagaa ccaagagtac caacccggca ttattttgat tagagaacac atgaacaaaa
     6721 caggtaagag aaggaacatt gcatctctca acgtgatcga caggttcata actcgactgc
     6781 tgtcgcaaaa gttgaataga tacttagccc ccattttctg cgagaactca tacgcttacc
     6841 aagactcaaa aggggtgatg cccgccgtac taaaagccaa ggagtacgtc gagttgggta
     6901 tgagacacgt tatcgagata gacctcaaga attacttcga cacgatcccg ttagagaact
     6961 taatcccaga gatcgagcgt tacataacag atgaggcagt gctgcacttg attaaacagt
     7021 acttattctg tgacatctcg ttcgagggta agatatcacg taaaactcaa gggatcgttc
     7081 agggtaacgc aatttcccca atactatcca acctctacct gaacgacttc gacaaggagc
     7141 tggacgagtc gaagctgtgc tggatcagat acgccgacaa catatacatc tatatggaca
     7201 gctacgagaa ggccctactg gtttattccg agcttaccga gagacttgag agaaggaaat
     7261 taaccgttaa caaggagaaa tcaggggtgt ttgatgttag tacccgttct atactaggtt
     7321 acgacatcct gatcagaaac aagaaggttg acgtgcgtaa acacatctac aagtctgtta
     7381 accagtactc caactggcac gactctcgat tggagttcat caacggtcgt taccacatca
     7441 cttccgacgg tatcctcaat agacaagact tcggtctact gttcgagaat gagcagaaga
     7501 agcactacat acctgttgag gtttctgacc agttgaatat ttacggaaac gttacgctcg
     7561 ccagcaacgt tcttcaatca ttctccaacc gagagatcaa agtgtccttc ttcgacaagt
     7621 acgggagact tattggatct ttcctgcccg agaaaactaa gaaaagcgcc gagataatac
     7681 tggttcagtc taagaactac cttaacgagg acgttaggat ggacacggcc cgacgtatgg
     7741 agatcgccgg tttgcataac atcagagcca acctcagata ctacgacaag aagcacaaag
     7801 gtgactttaa ggaaaaggta gacgccattt ctggctatat tgacgccctc aatagagcac
     7861 catccgtgaa cgacatgatg ctcttagagg ctaaagctcg tcagctgtac tacacatgct
     7921 tcaaccagat cttagagaca tccgactttc agttcgagaa gcgtaccaag cgaccaccaa
     7981 aagacgccat caacgcctgt atttcattcg gcaacacatt actgtacaac ttgtttgtga
     8041 atatcatttg gaagaaggga cttgatccaa ggttcggggt tgttcacgcc agcaacaagc
     8101 gtaatcagtc ccttaacctg gattttgctg acatcttcaa gcctatagtc atagatagga
     8161 tcatcttcac aatgatcaac aagaagatgc tgaccttgtt gacggacttc gagacttcta
     8221 accagggagt ttacctttcc cgagagggga agaacatctt cttgcagatg tacgaagaaa
     8281 agctcaaatc cagaatcact atcaagggaa aggagatgag ttactatcag ttgctagaat
     8341 cagaggtgca gaactacaag aacttcatcc tgaccggcga gacatacaag ccgtacaagt
     8401 actactagcg tacgttaact ttaagaagga gaacttaaga tgtatgtcat tctagtttac
     8461 gacattcacc agaagcgagt aggtaaggct ctaaagattt gtaggaagta cctgatccac
     8521 atccagaagt cagttttcga aggcaacata acggagagta agctaaaggc cttaaaagag
     8581 gagctggggc acctgatcga cactcagatg gactcggtaa ttatctacca cctggactcc
     8641 gtgaagtaca ctaagaagga gcagatcggc atcgtccaat cgacatccaa tgtcatttga
     8701 ctcgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg
     8761 agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga
     8821 aaggaggaac tatatccgga taccaggtgc ggggaagctt atgttccata gcaaaaagtc
     8881 ggtcagtctc gtggctgaaa tcatgagttc cacaaaatgg ctgaaattca aggaaaatca
     8941 ggaatctcag aaaaacgatc gaccgacttt ttcgataaaa tggttgcaaa aatgagaaaa
     9001 atctgattta atagaatctg aaaacagcgg aaatgctgtt gtcgtacttt acctaaaagg
     9061 aattgaaacg tcccgc
//
