LOCUS       sequence-227017-        5207 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector to express GFP (under the control of the DSCP and an array of
            4 UAS sites).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5207)
  AUTHORS   Arnold CD, Nemcko F, Woodfin AR, Wienerroither S, Vlasova A, 
            Schleiffer A, Pagani M, Rath M, Stark A
  TITLE     A high-throughput method to identify trans-activation domains within
            transcription factor sequences.
  JOURNAL   EMBO J. 2018 Aug 15;37(16). pii: embj.201798896. doi: 
            10.15252/embj.201798896. Epub 2018 Jul 13.
  PUBMED    30006452
REFERENCE   2  (bases 1 to 5207)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2018-08-15"; volume: "37(16). pii: embj.201798896. doi"; 
            pages: " 10.15252/embj.201798896. Epub 2018 Jul 13"
COMMENT     Sequence Label: pGL4.26_4xUAS_DSCP_GFP
FEATURES             Location/Qualifiers
     source          1..5207
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(11..802,803..871))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    976..1024
                     /note="synthetic polyadenylation signal"
     misc_feature    1038..1129
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     1078..1097
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     protein_bind    1176..1200
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1305..1329)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1526..1535
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1565..1593)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2188..2208
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     polyA_signal    2291..2412
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2302..2321)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2356..2375
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        2606..2963
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        2606..2797
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(2629..2649)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      2814..2949
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2876..2895
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             2994..4031
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     EVGRV"
     RBS             4010..4018
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    4055..4103
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(4259..4276)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4430..5018)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4510..4529)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 actgcagtgg ttaccagtgc ttgatcagtg aggcaccgat ctcagcgatc tgcctatttc
       61 gttcgtccat agtggcctga ctccccgtcg tgtagatcac tacgattcgt gagggcttac
      121 catcaggccc cagcgcagca atgatgccgc gagagccgcg ttcaccggcc cccgatttgt
      181 cagcaatgaa ccagccagca gggagggccg agcgaagaag tggtcctgct actttgtccg
      241 cctccatcca gtctatgagc tgctgtcgtg atgctagagt aagaagttcg ccagtgagta
      301 gtttccgaag agttgtggcc attgctactg gcatcgtggt atcacgctcg tcgttcggta
      361 tggcttcgtt caactctggt tcccagcggt caagccgggt cacatgatca cccatattat
      421 gaagaaatgc agtcagctcc ttagggcctc cgatcgttgt cagaagtaag ttggccgcgg
      481 tgttgtcgct catggtaatg gcagcactac acaattctct taccgtcatg ccatccgtaa
      541 gatgcttttc cgtgaccggc gagtactcaa ccaagtcgtt ttgtgagtag tgtatacggc
      601 gaccaagctg ctcttgcccg gcgtctatac gggacaacac cgcgccacat agcagtactt
      661 tgaaagtgct catcatcggg aatcgttctt cggggcggaa agactcaagg atcttgccgc
      721 tattgagatc cagttcgata tagcccactc ttgcacccag ttgatcttca gcatctttta
      781 ctttcaccag cgtttcgggg tgtgcaaaaa caggcaagca aaatgccgca aagaagggaa
      841 tgagtgcgac acgaaaatgt tggatgctca tactcgtcct ttttcaatat tattgaagca
      901 tttatcaggg ttactagtac gtctctcaag gataagtaag taatattaag gtacgggagg
      961 tattggacag gccgcaataa aatatcttta ttttcattac atctgtgtgt tggttttttg
     1021 tgtgaatcga tagtactaac atacgctctc catcaaaaca aaacgaaaca aaacaaacta
     1081 gcaaaatagg ctgtccccag tgcaagtgca ggtgccagaa catttctctg gcctaactgg
     1141 ccggtacctg agctcgctag cctcgaggat atcaaacaag tttgtacaaa aaagcaggct
     1201 tccgggagta ctgtcctccg agcggagtac tgtcctccga gcgggaagct tgcatgcctg
     1261 caggtcggag tactgtcctc cgagcggagt actgtcctcc gaggacccag ctttcttgta
     1321 caaagtggta tatcaagatc tgcgatcgag ctcgcccggg gatcgagcgc agcggtataa
     1381 aagggcgcgg ggtggctgag agcatcagtt gtgaatgaat gttcgagccg agcagacgtg
     1441 ccgctgcctt cgttaatatc ctttgaataa gccaactttg aatcacaaga cgcataccaa
     1501 acggcattcc ggtactgttg gtaaagccac catggctagc aaaggagaag aactcttcac
     1561 tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aacggccaca agttctctgt
     1621 cagtggagag ggtgaaggtg atgcaacata cggaaaactt accctgaagt tcatctgcac
     1681 tactggcaaa ctgcctgttc cctggccaac actagtcact actctgtgct atggtgttca
     1741 atgcttttca agatacccgg atcatatgaa acggcatgac tttttcaaga gtgccatgcc
     1801 cgaaggttat gtacaggaaa ggaccatctt cttcaaagat gacggcaact acaagacacg
     1861 tgctgaagtc aagtttgaag gtgataccct tgttaataga atcgagttaa aaggtattga
     1921 cttcaaggaa gatggcaaca ttctgggaca caaattggaa tacaactata actcacacaa
     1981 tgtatacatc atggcagaca aacaaaagaa tggaatcaaa gcgaacttca agacccgcca
     2041 caacattgaa gatggaagcg ttcaactagc agaccattat caacaaaata ctccaattgg
     2101 cgatggccct gtccttttac cagacaacca ttacctgtcc acacaatctg ccctttcgaa
     2161 agatcccaac gaaaagagag accacatggt ccttcttgag tttgtaacag ctgctgggat
     2221 tacacatggc atggatgaac tgtacaactg atctagagtc ggggcggccg gccgcttcga
     2281 gcagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
     2341 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
     2401 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg
     2461 tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtaaaatcga taaggatccg
     2521 tttgcgtatt gggcgctctt ccgctgatct gcgcagcacc atggcctgaa ataacctctg
     2581 aaagaggaac ttggttagct accttctgag gcggaaagaa ccagctgtgg aatgtgtgtc
     2641 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     2701 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     2761 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     2821 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     2881 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
     2941 ttggaggcct aggcttttgc aaaaagctcg attcttctga cactagcgcc accatgaaga
     3001 agcccgaact caccgctacc agcgttgaaa aatttctcat cgagaagttc gacagtgtga
     3061 gcgacctgat gcagttgtcg gagggcgaag agagccgagc cttcagcttc gatgtcggcg
     3121 gacgcggcta tgtactgcgg gtgaatagct gcgctgatgg cttctacaaa gaccgctacg
     3181 tgtaccgcca cttcgccagc gctgcactac ccatccccga agtgttggac atcggcgagt
     3241 tcagcgagag cctgacatac tgcatcagta gacgcgccca aggcgttact ctccaagacc
     3301 tccccgaaac agagctgcct gctgtgttac agcctgtcgc cgaagctatg gatgctattg
     3361 ccgccgccga cctcagtcaa accagcggct tcggcccatt cgggccccaa ggcatcggcc
     3421 agtacacaac ctggcgggat ttcatttgcg ccattgctga tccccatgtc taccactggc
     3481 agaccgtgat ggacgacacc gtgtccgcca gcgtagctca agccctggac gaactgatgc
     3541 tgtgggccga agactgtccc gaggtgcgcc acctcgtcca tgccgacttc ggcagcaaca
     3601 acgtcctgac cgacaacggc cgcatcaccg ccgtaatcga ctggtccgaa gctatgttcg
     3661 gggacagtca gtacgaggtg gccaacatct tcttctggcg gccctggctg gcttgcatgg
     3721 agcagcagac tcgctacttc gagcgccggc atcccgagct ggccggcagc cctcgtctgc
     3781 gagcctacat gctgcgcatc ggcctggatc agctctacca gagcctcgtg gacggcaact
     3841 tcgacgatgc tgcctgggct caaggccgct gcgatgccat cgtccgcagc ggggccggca
     3901 ccgtcggtcg cacacaaatc gctcgccgga gcgcagccgt atggaccgac ggctgcgtcg
     3961 aggtgctggc cgacagcggc aaccgccggc ccagtacacg accgcgcgct aaggaggtag
     4021 gtcgagttta aactctagaa ccggtcatgg ccgcaataaa atatctttat tttcattaca
     4081 tctgtgtgtt ggttttttgt gtgttcgaac tagatgctgt cgaccgatgc ccttgagagc
     4141 cttcaaccca gtcagctcct tccggtgggc gcggggcatg actatcgtcg ccgcacttat
     4201 gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct tccgcttcct
     4261 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     4321 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     4381 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     4441 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     4501 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     4561 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     4621 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     4681 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     4741 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     4801 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     4861 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     4921 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     4981 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     5041 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     5101 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     5161 gtatatatga gtaaacttgg tctgacagcg gccgcaaatg ctaaacc
//
