LOCUS       sequence-227039-       12112 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses FsRT-Cas1-Cas2 under pT7lac, Fluc under pLuxR and Rluc 
            under ptetA promoter, encodes FsCRISPR array 2, compatible with 
            SENECA acquisition readout.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 4453 to 4487)
  AUTHORS   Schmidt F, Cherepkova MY, Platt RJ
  TITLE     Transcriptional recording by CRISPR spacer acquisition from RNA.
  JOURNAL   Nature. 2018 Oct;562(7727):380-385. doi: 10.1038/s41586-018-0569-1. 
            Epub 2018 Oct 3.
  PUBMED    30283135
REFERENCE   2  (bases 1 to 12112)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-018-0569-1"; journalName: "Nature"; date: "2018-10";
            volume: "562"; issue: "7727"; pages: "380-385"
COMMENT     Sequence Label: pFS_0414_pET30_MCS-BBaJ23100-LuxR-B0015term-pL...
FEATURES             Location/Qualifiers
     source          1..12112
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(210..1025)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(210..1025)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     933..952
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1017..1036
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      1147..1735
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1636..1655
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1889..1906
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    1921..2060
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(1986..2005)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2146..2168
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2162..2353)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(3162..4244)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     4206..4225
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(4245..4322)
                     /gene="lacI"
                     /label=lacI promoter
     promoter        4453..4487
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     RBS             4496..4507
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      5286..5357
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5373..5400
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             5483..7135
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(5549..5567)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      7152..7238
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     RBS             7313..7324
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             7331..7954
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(7404..7423)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     terminator      7999..8070
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      8086..8113
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     protein_bind    8128..8146
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8153..8171
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             8190..9125
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     terminator      9147..9233
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     9351..9370
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     9458..9477
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9458..9476
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    9477..9501
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             9516..9538
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(11791..11809)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      11805..11852
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ggccgctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
       61 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag
      121 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
      181 caaatatgta tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac
      241 tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat
      301 gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg
      361 attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta
      421 tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc
      481 atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca
      541 tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg
      601 ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca
      661 tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg
      721 gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc
      781 ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg
      841 gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat
      901 cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa
      961 tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg
     1021 ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa
     1081 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     1141 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     1201 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     1261 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     1321 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     1381 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     1441 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     1501 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     1561 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     1621 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     1681 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     1741 cagcaacgcg gcctttttac ggttcctggt cttttgctgg ccttttgctc acatgttctt
     1801 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     1861 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     1921 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca atggtgcact
     1981 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac
     2041 gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     2101 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     2161 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag
     2221 cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt
     2281 tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt
     2341 cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac
     2401 cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac
     2461 tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca
     2521 ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc
     2581 agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca
     2641 gactttacga aacacggaaa ccgaagatca ttcatgttgt tgctcaggtc gcagacgttt
     2701 tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa
     2761 ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg
     2821 gggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag
     2881 tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca
     2941 tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct
     3001 gtcctacgag ttgcatgata aagaagatag tcataagtgc ggcgacgata gtcatgcccc
     3061 gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgagatcccg
     3121 gtgcctaatg agtgagctaa cttacattaa ttgcgttgcg ctcactgccc gctttccagt
     3181 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     3241 tgcgtattgg gcgccagggt ggtttttctt ttcaccagtg agacgggcaa cagctgattg
     3301 cccttcaccg cctggccctg agagagttgc agcaagcggt ccacgctggt ttgccccagc
     3361 aggcgaaaat cctgtttgat ggtggttaac ggcgggatat aacatgagct atcttcggta
     3421 tcgtcgtatc ccactaccga gatgtccgca ccaacgcgca gcccggactc ggtaatggcg
     3481 cgcattgcgc ccagcgccat ctgatcgttg gcaaccagca tcgcagtggg aacgatgccc
     3541 tcattcagca tttgcatggt ttgttgaaaa ccggacatgg cactccagtc gccttcccgt
     3601 tccgctatcg gctgaatttg attgcgagtg agatatttat gccagccagc cagacgcaga
     3661 cgcgccgaga cagaacttaa tgggcccgct aacagcgcga tttgctggtg acccaatgcg
     3721 accagatgct ccacgcccag tcgcgtacca tcttcatggg agaaaataat actgttgatg
     3781 ggtgtctggt cagagacatc aagaaataac gccggaacat tagtgcaggc agcttccaca
     3841 gcaatggcat cctggtcatc cagcggatag ttaatgatca gcccactgac gcgttgcgcg
     3901 agaagattgt gcaccgccgc tttacaggct tcgacgccgc ttcgttctac catcgacacc
     3961 accacgctgg cacccagttg atcggcgcga gatttaatcg ccgcgacaat ttgcgacggc
     4021 gcgtgcaggg ccagactgga ggtggcaacg ccaatcagca acgactgttt gcccgccagt
     4081 tgttgtgcca cgcggttggg aatgtaattc agctccgcca tcgccgcttc cactttttcc
     4141 cgcgttttcg cagaaacgtg gctggcctgg ttcaccacgc gggaaacggt ctgataagag
     4201 acaccggcat actctgcgac atcgtataac gttactggtt tcacattcac caccctgaat
     4261 tgactctctt ccgggcgcta tcatgccata ccgcgaaagg ttttgcgcca ttcgatggtg
     4321 tccgggatct cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag
     4381 gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaactagt atacagtgac
     4441 tcttggcgcg ccttgacggc tagctcagtc ctaggtacag tgctagctac tagagaaaga
     4501 ggagaaatac tagatgaaaa acataaatgc cgacgacaca tacagaataa ttaataaaat
     4561 taaagcttgt agaagcaata atgatattaa tcaatgctta tctgatatga ctaaaatggt
     4621 acattgtgaa tattatttac tcgcgatcat ttatcctcat tctatggtta aatctgatat
     4681 ttcaatccta gataattacc ctaaaaaatg gaggcaatat tatgatgacg ctaatttaat
     4741 aaaatatgat cctatagtag attattctaa ctccaatcat tcaccaatta attggaatat
     4801 atttgaaaac aatgctgtaa ataaaaaatc tccaaatgta attaaagaag cgaaaacatc
     4861 aggtcttatc actgggttta gtttccctat tcatacggct aacaatggct tcggaatgct
     4921 tagttttgca cattcagaaa aagacaacta tatagatagt ttatttttac atgcgtgtat
     4981 gaacatacca ttaattgttc cttctctagt tgataattat cgaaaaataa atatagcaaa
     5041 taataaatca aacaacgatt taaccaaaag agaaaaagaa tgtttagcgt gggcatgcga
     5101 aggaaaaagc tcttgggata tttcaaaaat attaggttgc agtgagcgta ctgtcacttt
     5161 ccatttaacc aatgcgcaaa tgaaactcaa tacaacaaac cgctgccaaa gtatttctaa
     5221 agcaatttta acaggagcaa ttgattgccc atactttaaa aattaataat actagagcca
     5281 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
     5341 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg
     5401 tttataatta aaggcatgaa ttcacctgta ggatcgtaca ggtttacgca agaaaatggt
     5461 ttgttatagt cgaataaata ctatggaaga tgccaaaaac ataaagaaag gcccggcgcc
     5521 attctatccg ctggaagatg gaaccgctgg agagcaactg cataaggcta tgaagagata
     5581 cgccctggtt cctggaacaa ttgcttttac agatgcacat atcgaggtgg acatcactta
     5641 cgctgagtac ttcgaaatgt ccgttcggtt ggcagaagct atgaaacgat atgggctgaa
     5701 tacaaatcac agaatcgtcg tatgcagtga aaactctctt caattcttta tgccggtgtt
     5761 gggcgcgtta tttatcggag ttgcagttgc gcccgcgaac gacatttata atgaacgtga
     5821 attgctcaac agtatgggca tttcgcagcc taccgtggtg ttcgtttcca aaaaggggtt
     5881 gcaaaaaatt ttgaacgtgc aaaaaaagct cccaatcatc caaaaaatta ttatcatgga
     5941 ttctaaaacg gattaccagg gatttcagtc gatgtacacg ttcgtcacat ctcatctacc
     6001 tcccggtttt aatgaatacg attttgtgcc agagtccttc gatagggaca agacaattgc
     6061 actgatcatg aactcctctg gatctactgg tctgcctaaa ggtgtcgctc tgcctcatag
     6121 aactgcctgc gtgagattct cgcatgccag agatcctatt tttggcaatc aaatcattcc
     6181 ggatactgcg attttaagtg ttgttccatt ccatcacggt tttggaatgt ttactacact
     6241 cggatatttg atatgtggat ttcgagtcgt cttaatgtat agatttgaag aagagctgtt
     6301 tctgaggagc cttcaggatt acaagattca aagtgcgctg ctggtgccaa ccctattctc
     6361 cttcttcgcc aaaagcactc tgattgacaa atacgattta tctaatttac acgaaattgc
     6421 ttctggtggc gctcccctct ctaaggaagt cggggaagcg gttgccaaga ggttccatct
     6481 gccaggtatc aggcaaggat atgggctcac tgagactaca tcagctattc tgattacacc
     6541 cgagggggat gataaaccgg gcgcggtcgg taaagttgtt ccattttttg aagcgaaggt
     6601 tgtggatctg gataccggga aaacgctggg cgttaatcaa agaggcgaac tgtgtgtgag
     6661 aggtcctatg attatgtccg gttatgtaaa caatccggaa gcgaccaacg ccttgattga
     6721 caaggatgga tggctacatt ctggagacat agcttactgg gacgaagacg aacacttctt
     6781 catcgttgac cgcctgaagt ctctgattaa gtacaaaggc tatcaggtgg ctcccgctga
     6841 attggaatcc atcttgctcc aacaccccaa catcttcgac gcaggtgtcg caggtcttcc
     6901 cgacgatgac gccggtgaac ttcccgccgc cgttgttgtt ttggagcacg gaaagacgat
     6961 gacggaaaaa gagatcgtgg attacgtcgc cagtcaagta acaaccgcga aaaagttgcg
     7021 cggaggagtt gtgtttgtgg acgaagtacc gaaaggtctt accggaaaac tcgacgcaag
     7081 aaaaatcaga gagatcctca taaaggccaa gaagggcgga aagatcgccg tgtaatgacg
     7141 attaaaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     7201 gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgccctag acagctgggg
     7261 tcgactggca cgtaagaggt tccaactttc accataatga aacatactag agaaagagga
     7321 gaaatactag atgtccagat tagataaaag taaagtgatt aacagcgcat tagagctgct
     7381 taatgaggtc ggaatcgaag gtttaacaac ccgtaaactc gcccagaagc taggtgtaga
     7441 gcagcctaca ttgtattggc atgtaaaaaa taagcgggct ttgctcgacg ccttagccat
     7501 tgagatgtta gataggcacc atactcactt ttgcccttta gaaggggaaa gctggcaaga
     7561 ttttttacgt aataacgcta aaagttttag atgtgcttta ctaagtcatc gcgatggagc
     7621 aaaagtacat ttaggtacac ggcctacaga aaaacagtat gaaactctcg aaaatcaatt
     7681 agccttttta tgccaacaag gtttttcact agagaatgca ttatatgcac tcagcgctgt
     7741 ggggcatttt actttaggtt gcgtattgga agatcaagag catcaagtcg ctaaagaaga
     7801 aagggaaaca cctactactg atagtatgcc gccattatta cgacaagcta tcgaattatt
     7861 tgatcaccaa ggtgcagagc cagccttctt attcggcctt gaattgatca tatgcggatt
     7921 agaaaaacaa cttaaatgtg aaagtgggtc ctaataacac tgatagtgct agtgtagatc
     7981 actactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
     8041 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
     8101 gggcctttct gcgtttatat actagagtcc ctatcagtga tagagattga catccctatc
     8161 agtgatagag atactgagca ctactagaga tggcttccaa ggtgtacgac cccgagcaac
     8221 gcaaacgcat gatcactggg cctcagtggt gggctcgctg caagcaaatg aacgtgctgg
     8281 actccttcat caactactat gattccgaga agcacgccga gaacgccgtg atttttctgc
     8341 atggtaacgc tgcctccagc tacctgtgga ggcacgtcgt gcctcacatc gagcccgtgg
     8401 ctagatgcat catccctgat ctgatcggaa tgggtaagtc cggcaagagc gggaatggct
     8461 catatcgcct cctggatcac tacaagtacc tcaccgcttg gttcgagctg ctgaaccttc
     8521 caaagaaaat catctttgtg ggccacgact ggggggcttg tctggccttt cactactcct
     8581 acgagcacca agacaagatc aaggccatcg tccatgctga gagtgtcgtg gacgtgatcg
     8641 agtcctggga cgagtggcct gacatcgagg aggatatcgc cctgatcaag agcgaagagg
     8701 gcgagaaaat ggtgcttgag aataacttct tcgtcgagac catgctccca agcaagatca
     8761 tgcggaaact ggagcctgag gagttcgctg cctacctgga gccattcaag gagaagggcg
     8821 aggttagacg gcctaccctc tcctggcctc gcgagatccc tctcgttaag ggaggcaagc
     8881 ccgacgtcgt ccagattgtc cgcaactaca acgcctacct tcgggccagc gacgatctgc
     8941 ctaagatgtt catcgagtcc gaccctgggt tcttttccaa cgctattgtc gagggagcta
     9001 agaagttccc taacaccgag ttcgtgaagg tgaagggcct ccacttcagc caggaggacg
     9061 ctccagatga aatgggtaag tacatcaaga gcttcgtgga gcgcgtgctg aagaacgagc
     9121 agtaatgaga tcggatctcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
     9181 cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccggatc
     9241 ccatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg
     9301 ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg
     9361 gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg
     9421 gcgtagagga tcgagatcga tctcgatccc gcgaaattaa tacgactcac tataggggaa
     9481 ttgtgagcgg ataacaattc ccctctagaa ataattttgt ttaactttaa gaaggagata
     9541 tacatatgtt cactatagac gagatgctat caaagaacaa ccaaagattg gctttcgagc
     9601 acttcgctac taagaacgac ggatgtggcc ctgatggtat gcacgtatct gagctggaga
     9661 aatattggcg tatgaatcac gatcagatca tctccgactt gaagaaccaa gagtaccaac
     9721 ccggcattat tttgattaga gaacacatga acaaaacagg taagagaagg aacattgcat
     9781 ctctcaacgt gatcgacagg ttcataactc gactgctgtc gcaaaagttg aatagatact
     9841 tagcccccat tttctgcgag aactcatacg cttaccaaga ctcaaaaggg gtgatgcccg
     9901 ccgtactaaa agccaaggag tacgtcgagt tgggtatgag acacgttatc gagatagacc
     9961 tcaagaatta cttcgacacg atcccgttag agaacttaat cccagagatc gagcgttaca
    10021 taacagatga ggcagtgctg cacttgatta aacagtactt attctgtgac atctcgttcg
    10081 agggtaagat atcacgtaaa actcaaggga tcgttcaggg taacgcaatt tccccaatac
    10141 tatccaacct ctacctgaac gacttcgaca aggagctgga cgagtcgaag ctgtgctgga
    10201 tcagatacgc cgacaacata tacatctata tggacagcta cgagaaggcc ctactggttt
    10261 attccgagct taccgagaga cttgagagaa ggaaattaac cgttaacaag gagaaatcag
    10321 gggtgtttga tgttagtacc cgttctatac taggttacga catcctgatc agaaacaaga
    10381 aggttgacgt gcgtaaacac atctacaagt ctgttaacca gtactccaac tggcacgact
    10441 ctcgattgga gttcatcaac ggtcgttacc acatcacttc cgacggtatc ctcaatagac
    10501 aagacttcgg tctactgttc gagaatgagc agaagaagca ctacatacct gttgaggttt
    10561 ctgaccagtt gaatatttac ggaaacgtta cgctcgccag caacgttctt caatcattct
    10621 ccaaccgaga gatcaaagtg tccttcttcg acaagtacgg gagacttatt ggatctttcc
    10681 tgcccgagaa aactaagaaa agcgccgaga taatactggt tcagtctaag aactacctta
    10741 acgaggacgt taggatggac acggcccgac gtatggagat cgccggtttg cataacatca
    10801 gagccaacct cagatactac gacaagaagc acaaaggtga ctttaaggaa aaggtagacg
    10861 ccatttctgg ctatattgac gccctcaata gagcaccatc cgtgaacgac atgatgctct
    10921 tagaggctaa agctcgtcag ctgtactaca catgcttcaa ccagatctta gagacatccg
    10981 actttcagtt cgagaagcgt accaagcgac caccaaaaga cgccatcaac gcctgtattt
    11041 cattcggcaa cacattactg tacaacttgt ttgtgaatat catttggaag aagggacttg
    11101 atccaaggtt cggggttgtt cacgccagca acaagcgtaa tcagtccctt aacctggatt
    11161 ttgctgacat cttcaagcct atagtcatag ataggatcat cttcacaatg atcaacaaga
    11221 agatgctgac cttgttgacg gacttcgaga cttctaacca gggagtttac ctttcccgag
    11281 aggggaagaa catcttcttg cagatgtacg aagaaaagct caaatccaga atcactatca
    11341 agggaaagga gatgagttac tatcagttgc tagaatcaga ggtgcagaac tacaagaact
    11401 tcatcctgac cggcgagaca tacaagccgt acaagtacta ctagcgtacg ttaactttaa
    11461 gaaggagaac ttaagatgta tgtcattcta gtttacgaca ttcaccagaa gcgagtaggt
    11521 aaggctctaa agatttgtag gaagtacctg atccacatcc agaagtcagt tttcgaaggc
    11581 aacataacgg agagtaagct aaaggcctta aaagaggagc tggggcacct gatcgacact
    11641 cagatggact cggtaattat ctaccacctg gactccgtga agtacactaa gaaggagcag
    11701 atcggcatcg tccaatcgac atccaatgtc atttgactcg agatccggct gctaacaaag
    11761 cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg
    11821 gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatacc
    11881 aggtgcgggg aagcttatgt tccatagcaa aaagtcggtc agtctcgtgg ctgaaatcat
    11941 gagttccaca aaatggctga aattcaagga aaatcaggaa tctcagaaaa acgatcgacc
    12001 gactttttcg ataaaatggt tgcaaaaatg agaaaaatct gatttaatag aatctgaaaa
    12061 cagcggaaat gctgttgtcg tactttacct aaaaggaatt gaaacgtccc gc
//
