LOCUS       sequence-227066-        6595 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  miniTn5 plasmid to deliver constitutively expressed fluorescent 
            protein genes in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6595)
  AUTHORS   Schlechter RO, Jun H, Bernach M, Oso S, Boyd E, Munoz-Lintz DA, 
            Dobson RCJ, Remus DM, Remus-Emsermann MNP
  TITLE     Chromatic Bacteria - A Broad Host-Range Plasmid and Chromosomal 
            Insertion Toolbox for Fluorescent Protein Expression in Bacteria.
  JOURNAL   Front Microbiol. 2018 Dec 12;9:3052. doi: 10.3389/fmicb.2018.03052. 
            eCollection 2018.
  PUBMED    30631309
REFERENCE   2  (bases 1 to 6595)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol."; date: "2018-12-12"; volume: "9:3052. doi"; pages: " 
            10.3389/fmicb.2018.03052. eCollection 2018"
COMMENT     Sequence Label: pMRE-Tn5-146
FEATURES             Location/Qualifiers
     source          1..6595
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1426..1520
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             1564..2223
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1630..1649)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      2288..2374
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(4118..4136)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4204..4308
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4309..4377,4378..5169)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4527..4546)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     oriT            5408..5517
                     /note="incP origin of transfer"
     primer_bind     6071..6087
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6071..6087
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        join(6208..6213,6214..6230,6231..6236)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             join(6425..6595,1..363)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 tcccaacatc agccggactc cgattacctc gggaacttgc tccgtagtaa gacattcatc
       61 gcgcttgctg ccttcgacca agaagcggtt gttggcgctc tcgcggctta cgttctgccc
      121 aagtttgagc agccgcgtag tgagatctat atctatgatc tcgcagtctc cggcgagcac
      181 cggaggcagg gcattgccac cgcgctcatc aatctcctca agcatgaggc caacgcgctt
      241 ggtgcttatg tgatctacgt gcaagcagat tacggtgacg atcccgcagt ggctctctat
      301 acaaagttgg gcatacggga agaagtgatg cactttgata tcgacccaag taccgccacc
      361 taaagctcgc acgctgccgc aagcactcag ggcgcaaggg ctgctaaagg aagcggaaca
      421 cgtagaaagc cagtccgcag aaacggtgct gaccccggat gaatgtcagc tactgggcta
      481 tctggacaag ggaaaacgca agcgcaaaga gaaagcaggt agcttgcagt gggcttacat
      541 ggcgatagct agactgggcg gttttatgga cagcaagcga accggaattg ccagctgggg
      601 cgccctctgg taaggttggg aatccctgca aagtaaactg gatggctttc ttgccgccaa
      661 ggatctgatg gcgcagggga tcaagatctg atcaagagac aggatactag tggaggaaga
      721 aaaaatggtg agcaagggcg aggagctgat caaggagaac atgcacatga agctgtacat
      781 ggaaggcacc gtgaacaacc accacttcaa gtgcaccacc gaaggggagg gcaagcccta
      841 cgagggcacc cagacccaga ggattaaggt ggtggaggga ggccccctgc cgttcgcatt
      901 cgacatcctg gccacctgct ttatgtacgg gagcaagacc ttcatcaacc acacccaggg
      961 catccccgat ttctttaagc agtccttccc tgagggcttc acatgggaga gagtcaccac
     1021 atacgaagac gggggcgtgc ttaccgttac ccaggacacc agcctccagg acggctgctt
     1081 gatctacaac gtcaagctca gaggggtgaa cttcccatcc aacggccctg tgatgcagaa
     1141 gaaaacactc ggctgggagg ccaccaccga gaccctgtac cccgctgacg gcggcctgga
     1201 aggcagatgc gacatggccc tgaagctcgt gggcgggggc cacctgcact gcaacctgaa
     1261 gaccacatac agatccaaga aacccgctaa gaacctcaag atgcccggcg tctactttgt
     1321 ggaccgcaga ctggaaagaa tcaaggaggc cgacaatgag acctacgtcg agcagcacga
     1381 ggtggctgtg gccagatact gcgacctcct aattagctga gcttggactc ctgttgatag
     1441 atccagtaat gacctcagaa ctccatctgg atttgttcag aacgctcggt tgccgccggg
     1501 cgttttttat tggtgagaat ccaagctagc ttggcgagat tttcaggagc taaggaagct
     1561 aaaatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa
     1621 gaacattttg aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg
     1681 gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt
     1741 attcacattc ttgcccgcct gatgaatgct catccggaat ttcgtatggc aatgaaagac
     1801 ggtgagctgg tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact
     1861 gaaacgtttt catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata
     1921 tattcgcaag atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt
     1981 gagaatatgt ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac
     2041 gtggccaata tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa
     2101 ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc
     2161 catgtcggca gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg
     2221 taattttttt aaggcagtta ttggtgccct taaacgcctg gggtaatgac tctctagctt
     2281 gaggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     2341 tttgtcggtg aacgctctcc tgagtaggac aaatccgccg ctaggagctt gcggccgcca
     2401 ggatatcgac ccaagtaccg ccacctaaca attcgttcaa gccgagatcg gcttcccggc
     2461 cgacgcgtca attcgagcgc gtcaattcga gggccgcggc ctaggcggcc agatctgatc
     2521 aagagacagt ctagctagaa ttaattcgag tcgaccgatc ccgtacacaa gtagcgtcct
     2581 gaacggaacc tttcccgttt tccaggatct gatcttccat gtgacctcct aacatggtaa
     2641 cgttcatgat aacttctgct cttcatcgtg cggccgactg ggctaaatct gtgttctctt
     2701 cggcggcgct gggtgatcct cgccgtactg cccgcttggt taacgtcgcc gcccaattgg
     2761 caaaatattc tggtaaatca ataaccatct catcagaggg tagtgaagcc atgcaggaag
     2821 gcgcttaccg atttatccgc aatcccaacg tttctgccga ggcgatcaga aaggctggcg
     2881 ccatgcaaac agtcaagttg gctcaggagt ttcccgaact gctggccatt gaggacacca
     2941 cctctttgag ttatcgccac caggtcgccg aagagcttgg caagctgggc tctattcagg
     3001 ataaatcccg cggatggtgg gttcactccg ttctcttgct cgaggccacc acattccgca
     3061 ccgtaggatt actgcatcag gagtggtgga tgcgcccgga tgaccctgcc gatgcggatg
     3121 aaaaggagag tggcaaatgg ctggcagccg ccgcaactag ccggttacgc atgggcagca
     3181 tgatgagcaa cgtgattgcg gtctgtgacc gcgaagccga tattcatgct tatctgcagg
     3241 acaaactggc gcataacgag cgcttcgtgg tgcgctccaa gcacccacgc aaggacgtag
     3301 agtctgggtt gtatctgtac gaccatctga agaaccaacc ggagttgggt ggctatcaga
     3361 tcagcattcc gcaaaagggc gtggtggata aacgcggtaa acgtaaaaat cgaccagccc
     3421 gcaaggcgag cttgagcctg cgcagtgggc gcatcacgct aaaacagggg aatatcacgc
     3481 tcaacgcggt gctggccgag gagattaacc cgcccaaggg tgagaccccg ttgaaatggt
     3541 tgttgctgac cagcgaaccg gtcgagtcgc tagcccaagc cttgcgcgtc atcgacattt
     3601 atacccatcg ctggcggatc gaggagttcc ataaggcatg gaaaaccgga gcaggagccg
     3661 agaggcaacg catggaggag ccggataatc tggagcggat ggtctcgatc ctctcgtttg
     3721 ttgcggtcag gctgttacag ctcagagaaa gcttcacgct gccgcaagca ctcagggcgc
     3781 aagggctgct aaaggaagcg gaacacgtag aaagccagtc cgcagaaacg gtgctgaccc
     3841 cggatgaatg tcagctactg ggctatctgg acaagggaaa acgcaagcgc aaagagaaag
     3901 caggtagctt gcagtgggct tacatggcga tagctagact gggcggtttt atggacagca
     3961 agcgaaccgg aattgccagc tggggcgccc tctgggaagg ttgggaagcc ctgcaaagta
     4021 aactggatgg ctttcttgcc gccaaggatc tgatggcgca ggggatcaag atctgatcgg
     4081 tcgatcgaat taattcccaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta
     4141 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     4201 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     4261 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     4321 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     4381 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     4441 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     4501 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     4561 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     4621 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     4681 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     4741 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     4801 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     4861 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     4921 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     4981 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     5041 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     5101 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     5161 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     5221 ttttaattta aaaggatcta ggtgaagatc cggggaattg gggatcaatt ccgttttccg
     5281 ctgcataacc ctgcttcggg gtcattatag cgattttttc ggtatatcca tcctttttcg
     5341 cacgatatac aggattttgc caaagggttc gtgtagactt tccttggtgt atccaacggc
     5401 gtcagccggg caggataggt gaagtaggcc cacccgcgag cgggtgttcc ttcttcactg
     5461 tcccttattc gcacctggcg gtgctcaacg ggaatcctgc tctgcgaggc tggccggcta
     5521 ccgccggcgt aacagatgag ggcaagcgga tggctgatga aaccaagcca accaggaagg
     5581 gcagcccacc tatcacggaa ttgatccggc cacgatgcgt ccggcgtaga ggatctgaag
     5641 atcagcagtt caacctgttg atagtacgta ctaagctctc atgtttcacg tactaagctc
     5701 tcatgtttaa cgtactaagc tctcatgttt aacgaactaa accctcatgg ctaacgtact
     5761 aagctctcat gtttcacgta ctaagctctc atgtttgaac aataaaatta atataaatca
     5821 gcaacttaaa tagcctctaa ggttttaagt tttataagaa aaaaaagaat atataaggct
     5881 tttaaagctt ttaaggttta acggttgtgg acaacaagcc agggatgtaa cgcactgaga
     5941 agcccttaga gcctctcaaa gcaattttga gtgacacagg aacacttaac ggctgacatg
     6001 ggaattaatt ctgactctta tacacaagtg cggccctcga attgacgcgc tccaccgcgg
     6061 tggcggccgc tctagaacta gtggatcccc cgggctgcag gaattcgata tccataaact
     6121 gccaggaatt ggggatcgga ttcgagcttt agtacctaga tttagatgtc taaaaagcgg
     6181 ggtaccgagg acgcgtcaat tctcgaattg acataagcct gttcggttcg taaactgtaa
     6241 tgcaagtagc gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt
     6301 aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttgtaca gtctatgcct
     6361 cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc
     6421 aacgatgtta cgcagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt
     6481 aggtggctca agtatgggca tcattcgcac atgtaggctc ggccctgacc aagtcaaatc
     6541 catgcgggct gctcttgatc ttttcggtcg tgagttcgga gacgtagcca cctac
//
