LOCUS       sequence-227067-        7099 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N-terminus to aa468 of Bxb1 integrase fused to GID1 CID.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7099)
  AUTHORS   Weinberg BH, Cho JH, Agarwal Y, Pham NTH, Caraballo LD, Walkosz M, 
            Ortega C, Trexler M, Tague N, Law B, Benman WKJ, Letendre J, Beal J,
            Wong WW
  TITLE     High-performance chemical- and light-inducible recombinases in 
            mammalian cells and mice.
  JOURNAL   Nat Commun. 2019 Oct 24;10(1):4845. doi: 10.1038/s41467-019-12800-7.
  PUBMED    31649244
REFERENCE   2  (bases 1 to 7099)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-10-24"; volume: "10(1):4845. doi"; pages: " 
            10.1038/s41467-019-12800-7"
COMMENT     Sequence Label: pBW2121_pCAG-PV1-Bxb1-N468-L1-GID1-NLS-BGHpA
FEATURES             Location/Qualifiers
     source          1..7099
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     466..488
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     550..569
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             3158..3178
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    3188..3412
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(3652..3671)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3717..3772
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3771..3790)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4133..4149)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4133..4149)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4146..4168)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4157..4173
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4181..4187,4188..4205,4206..4211))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4226..4247
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      4332..4466
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4486..4620
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4523..4542)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4577..4596
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4688..4705)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4859..5447)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4939..4958)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5618..6409,6410..6478))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6241..6260
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6479..6583)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        6614..6993
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg
       61 ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg
      121 gggcggggcc gcctcgggcc ggggagggct cgggggaggg gcgcggcggc ccccggagcg
      181 ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga
      241 gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc
      301 gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg
      361 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct
      421 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc
      481 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac
      541 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattgatta
      601 attcgagcga aggcgcgccc attactcgca tccattctca ggctgtctcg tctcgtctcg
      661 cacgcgtgcc gccaccatgc gagccctggt ggtcattcgc ctgagcagag tcacagacgc
      721 tactacaagc cctgagcggc agctggagtc ctgtcagcag ctgtgcgcac agcgaggatg
      781 ggatgtggtc ggagtggcag aggatctgga cgtgagcggg gctgtcgatc cattcgaccg
      841 aaagcggaga cccaacctgg cacgatggct ggctttcgag gaacagccct ttgatgtgat
      901 cgtcgcctac agagtggaca ggctgacacg ctcaattcga catctgcagc agctggtgca
      961 ttgggccgag gatcacaaga aactggtggt cagcgcaact gaagcccact tcgacaccac
     1021 aactcctttt gccgctgtgg tcatcgcact gatgggcacc gtggcccaga tggagctgga
     1081 agctatcaag gagcgaaacc ggagcgcagc ccatttcaat attcgggccg ggaaatacag
     1141 aggcagcctg cccccttggg gctatctgcc tacccgggtg gatggggagt ggagactggt
     1201 gccagacccc gtccagagag agaggattct ggaagtgtac cacagagtgg tggacaacca
     1261 cgaaccactg catctggtgg cccacgatct gaataggcgc ggagtcctgt ctccaaagga
     1321 ctattttgct cagctgcagg gaagggagcc acagggacga gaatggagtg ctaccgcact
     1381 gaagcggtct atgatcagtg aggctatgct gggctatgca actctgaatg ggaaaaccgt
     1441 gagagacgat gacggagcac cactggtgcg ggctgagcct attctgacaa gagagcagct
     1501 ggaagctctg agggcagaac tggtgaaaac cagtagggcc aagcctgctg tgtcaacacc
     1561 aagcctgctg ctgcgagtgc tgttctgcgc agtctgtggc gagccagcat acaaatttgc
     1621 cggcggggga aggaagcatc cccgctatcg atgccggagc atggggttcc ctaagcactg
     1681 tggaaacggc actgtggcta tggccgaatg ggacgccttt tgtgaggaac aggtgctgga
     1741 tctgctgggg gacgcagagc gcctggaaaa agtgtgggtc gctggaagcg attccgctgt
     1801 ggagctggca gaagtcaatg ccgagctggt ggacctgacc tccctgatcg gatctcctgc
     1861 atacagggca ggctccccac agcgagaagc tctggatgca cgaattgctg cactggcagc
     1921 tcgacaggag gaactggagg ggctggaagc cagaccctct ggatgggagt ggcgagaaac
     1981 aggccagcgg tttggggatt ggtggaggga gcaggacaca gcagccaaga acacttggct
     2041 gagatccatg aatgtcaggc tgactttcga cgtgcgagga tccggagggt ctggctccgg
     2101 atcaagtggt ggcagcggta ccatggccgc cagcgacgaa gtgaacctga tcgagagcag
     2161 aaccgtggtg cccctgaaca cctgggtgct gatctccaac ttcaaggtgg cctacaacat
     2221 cctgcggagg cccgacggca ccttcaacag acacctggcc gagtacctgg accggaaagt
     2281 gaccgccaac gccaaccctg tggacggcgt gttcagcttc gacgtgctga tcgaccggcg
     2341 gatcaacctg ctgagccggg tgtacagacc cgcctacgcc gatcaggaac agcccccctc
     2401 tatcctggat ctggaaaagc ccgtggatgg cgacatcgtg cccgtgatcc tgttcttcca
     2461 cggcggcagc tttgcccaca gcagcgccaa tagcgccatc tacgacaccc tgtgcagacg
     2521 gctcgtgggc ctgtgcaaat gcgtggtggt gtccgtgaac taccgcagag cccccgagaa
     2581 cccttacccc tgcgcctacg atgatggctg gatcgccctg aactgggtca acagcagaag
     2641 ctggctgaag tccaagaaag acagcaaggt gcacatcttt ctggccggcg atagcagcgg
     2701 cggcaatatc gcccataacg tggccctgag agccggcgag tctggcatcg atgtgctggg
     2761 caatatcctg ctgaacccca tgttcggcgg caacgagcgg accgagagcg agaagtctct
     2821 ggacggcaag tacttcgtga ccgtgcggga ccgggactgg tactggaagg cctttctgcc
     2881 cgagggcgag gacagagagc accccgcctg caatcccttc agccccagag gcaaaagcct
     2941 ggaaggcgtg tccttcccaa agtccctggt ggtggtggcc ggcctggacc tgatcagaga
     3001 ttggcagctg gcctatgccg agggcctgaa gaaagccggc caggaagtga agctgatgca
     3061 cctggaaaag gccaccgtgg gcttctacct gctgcccaac aacaaccact tccacaacgt
     3121 gatggacgag atcagcgcct tcgtgaacgc cgagtgcccc aagaaaaagc ggaaggtgtg
     3181 agaattcctg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     3241 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     3301 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     3361 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     3421 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cgtgtaccgc
     3481 tgggagttcg tagacggaaa caaacgcaga atccaagcgc ggccgcccag gatacataga
     3541 ttaccacaac tccgagccct tccaccgcta gcgcactcct caggtgcagg ctgcctatca
     3601 gaaggtggtg gctggtgtgg ccaatgccct ggctcacaaa taccactgag atctttttcc
     3661 ctctgccaaa aattatgggg acatcatgaa gccccttgag catctgactt ctggctaata
     3721 aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag
     3781 gacatatggg agggcaaatc atttaaaaca tcagaatgag tatttggttt agagtttggc
     3841 aacatatgcc catatgctgg ctgccatgaa caaaggttgg ctataaagag gtcatcagta
     3901 tatgaaacag ccccctgctg tccattcctt attccataga aaagccttga cttgaggtta
     3961 gatttttttt atattttgtt ttgtgttatt tttttcttta acatccctaa aattttcctt
     4021 acatgtttta ctagccagat ttttcctcct ctcctgacta ctcccagtca tagctgtccc
     4081 tcttctctta tggagatccc tcgacctgca gcccaagctt ggcgtaatca tggtcatagc
     4141 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     4201 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     4261 cactgcccgc tttccagtcg ggaaacctgt cgtgccagcg gatccgcatc tcaattagtc
     4321 agcaaccata gtcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     4381 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     4441 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctaactt gtttattgca
     4501 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     4561 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
     4621 cgctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     4681 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     4741 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     4801 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     4861 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     4921 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     4981 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     5041 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     5101 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     5161 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     5221 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     5281 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     5341 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     5401 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     5461 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     5521 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     5581 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     5641 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     5701 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     5761 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     5821 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     5881 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     5941 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     6001 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     6061 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     6121 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     6181 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     6241 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     6301 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     6361 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     6421 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     6481 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     6541 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     6601 tgccacctgg gtcgacattg attattgact agttattaat agtaatcaat tacggggtca
     6661 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
     6721 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
     6781 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
     6841 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
     6901 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
     6961 tacatctacg tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc
     7021 actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta
     7081 ttttgtgcag cgatggggg
//
