LOCUS       sequence-227078-        3164 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses aa257 to C-terminus of Cre recombinase fused to pMag CID.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Weinberg BH, Cho JH, Agarwal Y, Pham NTH, Caraballo LD, Walkosz M, 
            Ortega C, Trexler M, Tague N, Law B, Benman WKJ, Letendre J, Beal J,
            Wong WW
  TITLE     High-performance chemical- and light-inducible recombinases in 
            mammalian cells and mice.
  JOURNAL   Nat Commun. 2019 Oct 24;10(1):4845. doi: 10.1038/s41467-019-12800-7.
  PUBMED    31649244
REFERENCE   2  (bases 1 to 3164)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-10-24"; volume: "10(1):4845. doi"; pages: " 
            10.1038/s41467-019-12800-7"
COMMENT     Sequence Label: pBW2698_pCAG-PV1-pMag-L1-iCre-257C-BGHpA
FEATURES             Location/Qualifiers
     source          1..3164
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     466..488
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     550..569
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1433..1453
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1463..1687
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(1927..1946)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    1992..2047
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2046..2065)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(2408..2424)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2408..2424)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2421..2443)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2432..2448
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2456..2462,2463..2480,2481..2486))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2501..2522
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      2607..2741
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    2761..2895
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2798..2817)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2852..2871
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2963..2980)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg
       61 ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg
      121 gggcggggcc gcctcgggcc ggggagggct cgggggaggg gcgcggcggc ccccggagcg
      181 ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga
      241 gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc
      301 gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg
      361 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct
      421 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc
      481 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac
      541 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattgatta
      601 attcgagcga aggcgcgccc attactcgca tccattctca ggctgtctcg tctcgtctcg
      661 cacgcgtgcc gccaccatgc atactcttta tgcccccggt ggatatgaca ttatgggata
      721 tctgaggcag atcaggaacc ggccaaaccc gcaggtggaa ctgggccccg tggatacatc
      781 ctgcgccttg attctttgtg acctgaaaca gaaagacacc ccgatagttt acgcgagtga
      841 agccttcctc tacatgacag gttacagcaa cgcagaggtg ctgggccgga attgccggtt
      901 tctgcaaagc cctgacggca tggtgaagcc caagagcacc cggaagtacg tggatagtaa
      961 cacaatcaat actatgcgca aggcaatcga caggaatgcc gaggtgcagg ttgaagtagt
     1021 caattttaaa aagaatggac agcgatttgt taatttcctg actatgatac ctgttaggga
     1081 cgaaacaggc gagtatcgat actctatggg attccagtgc gaaacagaat ccggagggtc
     1141 tggctccgga tcaagtggtg gcagcggtac ctccacccgg gccctggaag ggatctttga
     1201 ggccacccac cgcctgatct atggtgccaa ggatgactct gggcagagat acctggcctg
     1261 gtctggccac tctgccagag tgggtgctgc cagggacatg gccagggctg gtgtgtccat
     1321 ccctgaaatc atgcaggctg gtggctggac caatgtgaac attgtgatga actacatcag
     1381 aaacctggac tctgagactg gggccatggt gaggctgctc gaggatgggg accccaagaa
     1441 aaagcggaag gtgtgagaat tcctgtgcct tctagttgcc agccatctgt tgtttgcccc
     1501 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     1561 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     1621 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     1681 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
     1741 tgtagcgtgt accgctggga gttcgtagac ggaaacaaac gcagaatcca agcgcggccg
     1801 cccaggatac atagattacc acaactccga gcccttccac cgctagcgca ctcctcaggt
     1861 gcaggctgcc tatcagaagg tggtggctgg tgtggccaat gccctggctc acaaatacca
     1921 ctgagatctt tttccctctg ccaaaaatta tggggacatc atgaagcccc ttgagcatct
     1981 gacttctggc taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg
     2041 tctctcactc ggaaggacat atgggagggc aaatcattta aaacatcaga atgagtattt
     2101 ggtttagagt ttggcaacat atgcccatat gctggctgcc atgaacaaag gttggctata
     2161 aagaggtcat cagtatatga aacagccccc tgctgtccat tccttattcc atagaaaagc
     2221 cttgacttga ggttagattt tttttatatt ttgttttgtg ttattttttt ctttaacatc
     2281 cctaaaattt tccttacatg ttttactagc cagatttttc ctcctctcct gactactccc
     2341 agtcatagct gtccctcttc tcttatggag atccctcgac ctgcagccca agcttggcgt
     2401 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     2461 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     2521 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagcggatcc
     2581 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccagtt ccgcccattc
     2641 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     2701 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     2761 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     2821 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     2881 tatcatgtct ggatccgctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     2941 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     3001 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     3061 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     3121 cgttgctggc gtttttccat aggctccgcc cccctgacga gcat
//
