LOCUS       sequence-227088-        6299 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses aa28 to C-terminus of Flp recombinase fused to pMag CID.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6299)
  AUTHORS   Weinberg BH, Cho JH, Agarwal Y, Pham NTH, Caraballo LD, Walkosz M, 
            Ortega C, Trexler M, Tague N, Law B, Benman WKJ, Letendre J, Beal J,
            Wong WW
  TITLE     High-performance chemical- and light-inducible recombinases in 
            mammalian cells and mice.
  JOURNAL   Nat Commun. 2019 Oct 24;10(1):4845. doi: 10.1038/s41467-019-12800-7.
  PUBMED    31649244
REFERENCE   2  (bases 1 to 6299)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-10-24"; volume: "10(1):4845. doi"; pages: " 
            10.1038/s41467-019-12800-7"
COMMENT     Sequence Label: pBW2666_pCAG-PV1-pMag-L1-FlpO-28C-NLS-BGHpA
FEATURES             Location/Qualifiers
     source          1..6299
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     466..488
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     550..569
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             2360..2380
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2390..2614
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(2854..2873)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2919..2974
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2973..2992)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3335..3351)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3335..3351)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3348..3370)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3359..3375
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3383..3389,3390..3407,3408..3413))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3428..3449
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      3534..3668
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3688..3822
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3725..3744)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3779..3798
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3890..3907)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4061..4649)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4141..4160)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4820..5611,5612..5680))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5443..5462
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5681..5785)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        5816..6195
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg
       61 ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg
      121 gggcggggcc gcctcgggcc ggggagggct cgggggaggg gcgcggcggc ccccggagcg
      181 ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga
      241 gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc
      301 gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg
      361 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct
      421 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc
      481 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac
      541 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattgatta
      601 attcgagcga aggcgcgccc attactcgca tccattctca ggctgtctcg tctcgtctcg
      661 cacgcgtgcc gccaccatgc atactcttta tgcccccggt ggatatgaca ttatgggata
      721 tctgaggcag atcaggaacc ggccaaaccc gcaggtggaa ctgggccccg tggatacatc
      781 ctgcgccttg attctttgtg acctgaaaca gaaagacacc ccgatagttt acgcgagtga
      841 agccttcctc tacatgacag gttacagcaa cgcagaggtg ctgggccgga attgccggtt
      901 tctgcaaagc cctgacggca tggtgaagcc caagagcacc cggaagtacg tggatagtaa
      961 cacaatcaat actatgcgca aggcaatcga caggaatgcc gaggtgcagg ttgaagtagt
     1021 caattttaaa aagaatggac agcgatttgt taatttcctg actatgatac ctgttaggga
     1081 cgaaacaggc gagtatcgat actctatggg attccagtgc gaaacagaat ccggagggtc
     1141 tggctccgga tcaagtggtg gcagcggtac cggcgagaag atcgccagct gtgccgccga
     1201 gctgacctac ctgtgctgga tgatcaccca caacggcacc gccatcaaga gggccacctt
     1261 catgagctac aacaccatca tcagcaacag cctgagcttc gacatcgtga acaagagcct
     1321 gcagttcaag tacaagaccc agaaggccac catcctggag gccagcctga agaagctgat
     1381 ccccgcctgg gagttcacca tcatccctta caacggccag aagcaccaga gcgacatcac
     1441 cgacatcgtg tccagcctgc agctgcagtt cgagagcagc gaggaggccg acaagggcaa
     1501 cagccacagc aagaagatgc tgaaggccct gctgtccgag ggcgagagca tctgggagat
     1561 caccgagaag atcctgaaca gcttcgagta caccagcagg ttcaccaaga ccaagaccct
     1621 gtaccagttc ctgttcctgg ccacattcat caactgcggc aggttcagcg acatcaagaa
     1681 cgtggacccc aagagcttca agctggtgca gaacaagtac ctgggcgtga tcattcagtg
     1741 cctggtgacc gagaccaaga caagcgtgtc caggcacatc tactttttca gcgccagagg
     1801 caggatcgac cccctggtgt acctggacga gttcctgagg aacagcgagc ccgtgctgaa
     1861 gagagtgaac aggaccggca acagcagcag caacaagcag gagtaccagc tgctgaagga
     1921 caacctggtg cgcagctaca acaaggccct gaagaagaac gccccctacc ccatcttcgc
     1981 tatcaagaac ggccctaaga gccacatcgg caggcacctg atgaccagct ttctgagcat
     2041 gaagggcctg accgagctga caaacgtggt gggcaactgg agcgacaaga gggcctccgc
     2101 cgtggccagg accacctaca cccaccagat caccgccatc cccgaccact acttcgccct
     2161 ggtgtccagg tactacgcct acgaccccat cagcaaggag atgatcgccc tgaaggacga
     2221 gaccaacccc atcgaggagt ggcagcacat cgagcagctg aagggcagcg ccgagggcag
     2281 catcagatac cccgcctgga acggcatcat cagccaggag gtgctggact acctgagcag
     2341 ctacatcaac aggcggatcc ccaagaaaaa gcggaaggtg tgagaattcc tgtgccttct
     2401 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     2461 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     2521 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     2581 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     2641 ggctctaggg ggtatcccca cgcgccctgt agcgtgtacc gctgggagtt cgtagacgga
     2701 aacaaacgca gaatccaagc gcggccgccc aggatacata gattaccaca actccgagcc
     2761 cttccaccgc tagcgcactc ctcaggtgca ggctgcctat cagaaggtgg tggctggtgt
     2821 ggccaatgcc ctggctcaca aataccactg agatcttttt ccctctgcca aaaattatgg
     2881 ggacatcatg aagccccttg agcatctgac ttctggctaa taaaggaaat ttattttcat
     2941 tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg ggagggcaaa
     3001 tcatttaaaa catcagaatg agtatttggt ttagagtttg gcaacatatg cccatatgct
     3061 ggctgccatg aacaaaggtt ggctataaag aggtcatcag tatatgaaac agccccctgc
     3121 tgtccattcc ttattccata gaaaagcctt gacttgaggt tagatttttt ttatattttg
     3181 ttttgtgtta tttttttctt taacatccct aaaattttcc ttacatgttt tactagccag
     3241 atttttcctc ctctcctgac tactcccagt catagctgtc cctcttctct tatggagatc
     3301 cctcgacctg cagcccaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     3361 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     3421 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     3481 cgggaaacct gtcgtgccag cggatccgca tctcaattag tcagcaacca tagtcccgcc
     3541 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     3601 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     3661 tggaggccta ggcttttgca aaaagctaac ttgtttattg cagcttataa tggttacaaa
     3721 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     3781 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tccgctgcat taatgaatcg
     3841 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     3901 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     3961 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     4021 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     4081 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     4141 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     4201 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     4261 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     4321 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     4381 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     4441 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     4501 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     4561 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     4621 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     4681 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     4741 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     4801 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     4861 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     4921 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     4981 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     5041 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     5101 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     5161 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     5221 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     5281 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     5341 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     5401 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     5461 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     5521 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     5581 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     5641 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     5701 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     5761 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gggtcgacat
     5821 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
     5881 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
     5941 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
     6001 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
     6061 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
     6121 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
     6181 atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc
     6241 ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc agcgatggg
//
