LOCUS       sequence-227089-        8816 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of human Epac1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8816)
  AUTHORS   Wang Z, Dillon TJ, Pokala V, Mishra S, Labudda K, Hunter B, Stork PJ
  TITLE     Rap1-mediated activation of extracellular signal-regulated kinases 
            by cyclic AMP is dependent on the mode of Rap1 activation.
  JOURNAL   Mol Cell Biol. 2006 Mar;26(6):2130-45. doi: 
            10.1128/MCB.26.6.2130-2145.2006.
  PUBMED    16507992
REFERENCE   2  (bases 1 to 8816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.26.6.2130-2145"; journalName: "Mol Cell Biol"; date: 
            "2006-03"; volume: "26"; issue: "6"; pages: "2130-45"
COMMENT     Sequence Label: GFP-Epac1 CAAX
FEATURES             Location/Qualifiers
     source          1..8816
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        83..462
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        463..666
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     617..637
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     711..730
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        711..729
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(764..766,767..769,770..1480)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(764..766,767..769,770..1480)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(809..830)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1070..1089)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1417..1438
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        complement(4217..4235)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4218..4235)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4255..4272)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4261..4485
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4531..4959
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4618..4637)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4828..4849
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4968..4988)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4973..5302
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5153..5288
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5215..5234
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5369..6163
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5423..5442)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6033..6052
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6337..6458
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6374..6393)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6428..6447
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6507..6523)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6507..6523)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6520..6542)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6531..6547
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6555..6561,6562..6579,6580..6585))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6600..6621
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6738..6755)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6909..7497)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6989..7008)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7668..8459,8460..8528))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8291..8310
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8529..8633)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8708..8727)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat
       61 gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata gtaatcaatt
      121 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
      181 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
      241 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
      301 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
      361 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
      421 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
      481 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
      541 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
      601 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
      661 agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat taatacgact
      721 cactataggg agacccaagc ttggtaccga gctcggatcc accatggtga gcaagggcga
      781 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
      841 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
      901 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
      961 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     1021 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     1081 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     1141 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     1201 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     1261 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     1321 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     1381 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     1441 cgccgccggg atcactctcg gcatggacga gctgtacaag gggaattcaa tggtgttgag
     1501 aaggatgcac cggccccgaa gctgctccta ccagctgctg ctggagcacc agcgtccgag
     1561 ctgcatccag gggctgcgct ggacaccact caccaacagc gaggagtccc tggatttcag
     1621 cgagagcctg gagcaggcct ccacagagcg ggtgctcagg gctgggaggc agctgcatcg
     1681 gcatctgctg gccacctgcc caaacctcac ccgagaccgg aagtaccacc ttaggctcta
     1741 tcggcagtgc tgctctggcc gggagctggt ggatgggatc ttggccctgg gacttggggt
     1801 ccattcccgg agccaagttg tgggaatctg ccaggtgctg ctggatgaag gtgccctctg
     1861 ccatgtgaaa cacgactggg ccttccagga ccgagatgcc caattctacc ggttccccgg
     1921 gcccgagccc gagcccgtgg gaactcatga gatggaggag gagttggccg aagctgtggc
     1981 cctgctctcc cagcgggggc ctgacgccct gctcactgtg gcacttcgaa agcccccagg
     2041 tcagcgcacg gatgaagagc tggacctcat ctttgaggag ctgctgcaca tcaaggctgt
     2101 ggcccacctc tccaactcgg tgaagcgaga attagcggct gttctgctct ttgaaccaca
     2161 cagcaaggca gggaccgtgt tgttcagcca gggggacaag ggcacttcgt ggtacattat
     2221 ctggaaggga tctgtcaacg tggtgaccca tggcaagggg ctggtgacca ccctgcatga
     2281 gggagatgat tttggacagc tggctctggt gaatgatgca ccccgggcag ccaccatcat
     2341 cctgcgagaa gacaactgtc atttcctgcg tgtggacaag caggacttca accgtatcat
     2401 caaggatgtg gaggcaaaga ccatgcggct ggaagaacat ggcaaagtgg tgctggtgct
     2461 ggagagagcc tctcagggcg ccggcccttc ccgaccccca accccaggca ggaaccggta
     2521 tacagtgatg tctggcaccc cagagaagat cctagagctt ctgttggagg ccatgggacc
     2581 agattccagt gctcatgacc caacagagac attcctcagc gacttcctcc tgacccacag
     2641 ggtcttcatg cccagcgccc aactctgcgc tgcccttctg caccacttcc atgtggagcc
     2701 tgcgggtggc agcgagcagg agcgcagcac ctacgtctgc aacaagaggc agcagatctt
     2761 gcggctggtc agccagtggg tggccctgta tggctccatg ctccacactg accctgtggc
     2821 caccagcttc ctccagaaac tctcagacct ggtgggcagg gacacccgac tcagcaacct
     2881 gctgagggag cagtggccag agaggcggcg atgccacagg ttggagaatg gctgtgggaa
     2941 tgcatctcct cagatgaagg cccggaactt gcctgtttgg ctccccaacc aggacgagcc
     3001 ccttcctggc agcagctgtg ccatccaagt tggggataaa gtcccctatg acatctgccg
     3061 gccagaccac tcagtgttga ccctgcagct gcctgtgaca gcctccgtga gagaggtgat
     3121 ggcagcgttg gcccaggagg atggctggac caaggggcag gtgctggtga aggtcaattc
     3181 tgcaggtgat gccattggcc tgcagccaga tgcccgtggt gtggccacat ctctggggct
     3241 caatgagcgt ctctttgttg tcaacccaca ggaagcgcat gagctgatcc cacaccctga
     3301 ccagctgggg cccactgtgg gctctgctga ggggctggac ctggtgagtg ccaaggacct
     3361 ggcaggccag ctgacggacc acgactggag cctcttcaac agtatccacc aggtggagct
     3421 gatccactat gtgctgggcc cccagcatct gcgggatgtc accaccgcca acctggagcg
     3481 cttcatgcgc cgcttcaatg agctgcagta ctgggtggcc accgagctgt gtctctgccc
     3541 cgtgcccggc ccccgggccc agctgctcag gaagttcatt aagctggcgg cccacctcaa
     3601 ggagcagaag aatctcaatt ccttctttgc cgtcatgttt ggcctcagca actcggccat
     3661 cagccgccta gcccacacct gggagcggct gccccacaaa gtccggaagc tgtactccgc
     3721 cctcgagagg ctgctggatc cctcatggaa ccaccgggta taccgactgg ccctcgccaa
     3781 gctctcccct cctgtcatcc ccttcatgcc ccttcttctc aaagacatga ccttcattca
     3841 tgagggaaac cacacactag tggagaatct catcaacttt gagaagatga gaatgatggc
     3901 cagagccgcg cggatgctgc accactgccg aagccacaac cctgtgcctc tctcaccact
     3961 cagaagccga gtttcccacc tccacgagga cagccaggtg gcgaggattt ccacatgctc
     4021 ggagcagtcc ctgagcaccc ggagtccagc cagcacctgg gcttatgtcc agcagctgaa
     4081 ggtcattgac aaccagcggg aactctcccg cctctcccga gagctggagc cagtcgagaa
     4141 gatgagcaaa gatggtaaaa agaagaaaaa gaagtcaaag acaaagtgtg taattatgta
     4201 atctagaggg ccctattcta tagtgtcacc taaatgctag agctcgctga tcagcctcga
     4261 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     4321 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     4381 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     4441 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
     4501 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg
     4561 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     4621 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     4681 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     4741 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     4801 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     4861 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     4921 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca
     4981 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     5041 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     5101 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     5161 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     5221 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
     5281 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga
     5341 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc
     5401 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
     5461 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
     5521 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc
     5581 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
     5641 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
     5701 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
     5761 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
     5821 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
     5881 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
     5941 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
     6001 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
     6061 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
     6121 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc
     6181 gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc
     6241 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
     6301 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat
     6361 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     6421 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc
     6481 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     6541 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     6601 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     6661 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     6721 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     6781 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     6841 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     6901 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     6961 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     7021 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     7081 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     7141 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     7201 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     7261 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     7321 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     7381 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     7441 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     7501 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     7561 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     7621 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     7681 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     7741 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     7801 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     7861 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     7921 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     7981 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     8041 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     8101 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     8161 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     8221 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     8281 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     8341 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     8401 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     8461 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     8521 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     8581 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     8641 ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtgca
     8701 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg
     8761 tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggc
//
