LOCUS       sequence-227110-        8816 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of human Epac1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8816)
  AUTHORS   Wang Z, Dillon TJ, Pokala V, Mishra S, Labudda K, Hunter B, Stork PJ
  TITLE     Rap1-mediated activation of extracellular signal-regulated kinases 
            by cyclic AMP is dependent on the mode of Rap1 activation.
  JOURNAL   Mol Cell Biol. 2006 Mar;26(6):2130-45. doi: 
            10.1128/MCB.26.6.2130-2145.2006.
  PUBMED    16507992
REFERENCE   2  (bases 1 to 8816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.26.6.2130-2145"; journalName: "Mol Cell Biol"; date: 
            "2006-03"; volume: "26"; issue: "6"; pages: "2130-45"
COMMENT     Sequence Label: GFP-Epac1 SAAX
FEATURES             Location/Qualifiers
     source          1..8816
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        139..518
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        519..722
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     673..693
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     767..786
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        767..785
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(820..822,823..825,826..1536)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(820..822,823..825,826..1536)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(865..886)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1126..1145)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1473..1494
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        complement(4273..4291)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4274..4291)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4311..4328)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4317..4541
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4587..5015
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4674..4693)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4884..4905
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5024..5044)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5029..5358
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5209..5344
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5271..5290
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5425..6219
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5479..5498)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6089..6108
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6393..6514
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6430..6449)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6484..6503
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6563..6579)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6563..6579)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6576..6598)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6587..6603
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6611..6617,6618..6635,6636..6641))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6656..6677
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6794..6811)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6965..7553)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7045..7064)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7724..8515,8516..8584))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8347..8366
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8585..8689)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8764..8783)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga
       61 ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac
      121 gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg
      181 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
      241 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
      301 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
      361 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
      421 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
      481 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
      541 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
      601 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
      661 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
      721 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
      781 atagggagac ccaagcttgg taccgagctc ggatccacca tggtgagcaa gggcgaggag
      841 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
      901 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
      961 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
     1021 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     1081 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     1141 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     1201 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     1261 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
     1321 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     1381 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc
     1441 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     1501 gccgggatca ctctcggcat ggacgagctg tacaagggga attcaatggt gttgagaagg
     1561 atgcaccggc cccgaagctg ctcctaccag ctgctgctgg agcaccagcg tccgagctgc
     1621 atccaggggc tgcgctggac accactcacc aacagcgagg agtccctgga tttcagcgag
     1681 agcctggagc aggcctccac agagcgggtg ctcagggctg ggaggcagct gcatcggcat
     1741 ctgctggcca cctgcccaaa cctcacccga gaccggaagt accaccttag gctctatcgg
     1801 cagtgctgct ctggccggga gctggtggat gggatcttgg ccctgggact tggggtccat
     1861 tcccggagcc aagttgtggg aatctgccag gtgctgctgg atgaaggtgc cctctgccat
     1921 gtgaaacacg actgggcctt ccaggaccga gatgcccaat tctaccggtt ccccgggccc
     1981 gagcccgagc ccgtgggaac tcatgagatg gaggaggagt tggccgaagc tgtggccctg
     2041 ctctcccagc gggggcctga cgccctgctc actgtggcac ttcgaaagcc cccaggtcag
     2101 cgcacggatg aagagctgga cctcatcttt gaggagctgc tgcacatcaa ggctgtggcc
     2161 cacctctcca actcggtgaa gcgagaatta gcggctgttc tgctctttga accacacagc
     2221 aaggcaggga ccgtgttgtt cagccagggg gacaagggca cttcgtggta cattatctgg
     2281 aagggatctg tcaacgtggt gacccatggc aaggggctgg tgaccaccct gcatgaggga
     2341 gatgattttg gacagctggc tctggtgaat gatgcacccc gggcagccac catcatcctg
     2401 cgagaagaca actgtcattt cctgcgtgtg gacaagcagg acttcaaccg tatcatcaag
     2461 gatgtggagg caaagaccat gcggctggaa gaacatggca aagtggtgct ggtgctggag
     2521 agagcctctc agggcgccgg cccttcccga cccccaaccc caggcaggaa ccggtataca
     2581 gtgatgtctg gcaccccaga gaagatccta gagcttctgt tggaggccat gggaccagat
     2641 tccagtgctc atgacccaac agagacattc ctcagcgact tcctcctgac ccacagggtc
     2701 ttcatgccca gcgcccaact ctgcgctgcc cttctgcacc acttccatgt ggagcctgcg
     2761 ggtggcagcg agcaggagcg cagcacctac gtctgcaaca agaggcagca gatcttgcgg
     2821 ctggtcagcc agtgggtggc cctgtatggc tccatgctcc acactgaccc tgtggccacc
     2881 agcttcctcc agaaactctc agacctggtg ggcagggaca cccgactcag caacctgctg
     2941 agggagcagt ggccagagag gcggcgatgc cacaggttgg agaatggctg tgggaatgca
     3001 tctcctcaga tgaaggcccg gaacttgcct gtttggctcc ccaaccagga cgagcccctt
     3061 cctggcagca gctgtgccat ccaagttggg gataaagtcc cctatgacat ctgccggcca
     3121 gaccactcag tgttgaccct gcagctgcct gtgacagcct ccgtgagaga ggtgatggca
     3181 gcgttggccc aggaggatgg ctggaccaag gggcaggtgc tggtgaaggt caattctgca
     3241 ggtgatgcca ttggcctgca gccagatgcc cgtggtgtgg ccacatctct ggggctcaat
     3301 gagcgtctct ttgttgtcaa cccacaggaa gcgcatgagc tgatcccaca ccctgaccag
     3361 ctggggccca ctgtgggctc tgctgagggg ctggacctgg tgagtgccaa ggacctggca
     3421 ggccagctga cggaccacga ctggagcctc ttcaacagta tccaccaggt ggagctgatc
     3481 cactatgtgc tgggccccca gcatctgcgg gatgtcacca ccgccaacct ggagcgcttc
     3541 atgcgccgct tcaatgagct gcagtactgg gtggccaccg agctgtgtct ctgccccgtg
     3601 cccggccccc gggcccagct gctcaggaag ttcattaagc tggcggccca cctcaaggag
     3661 cagaagaatc tcaattcctt ctttgccgtc atgtttggcc tcagcaactc ggccatcagc
     3721 cgcctagccc acacctggga gcggctgccc cacaaagtcc ggaagctgta ctccgccctc
     3781 gagaggctgc tggatccctc atggaaccac cgggtatacc gactggccct cgccaagctc
     3841 tcccctcctg tcatcccctt catgcccctt cttctcaaag acatgacctt cattcatgag
     3901 ggaaaccaca cactagtgga gaatctcatc aactttgaga agatgagaat gatggccaga
     3961 gccgcgcgga tgctgcacca ctgccgaagc cacaaccctg tgcctctctc accactcaga
     4021 agccgagttt cccacctcca cgaggacagc caggtggcga ggatttccac atgctcggag
     4081 cagtccctga gcacccggag tccagccagc acctgggctt atgtccagca gctgaaggtc
     4141 attgacaacc agcgggaact ctcccgcctc tcccgagagc tggagccagt cgagaagatg
     4201 agcaaagatg gtaaaaagaa gaaaaagaag tcaaagacaa agtctgtaat tatgtaatct
     4261 agagggccct attctatagt gtcacctaaa tgctagagct cgctgatcag cctcgactgt
     4321 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     4381 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     4441 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     4501 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
     4561 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
     4621 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     4681 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     4741 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     4801 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     4861 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     4921 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     4981 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta
     5041 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     5101 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     5161 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     5221 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     5281 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     5341 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa
     5401 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
     5461 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     5521 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
     5581 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg
     5641 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     5701 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
     5761 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
     5821 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     5881 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     5941 aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
     6001 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     6061 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     6121 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     6181 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa
     6241 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct
     6301 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
     6361 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt
     6421 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     6481 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta
     6541 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     6601 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     6661 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     6721 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     6781 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     6841 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     6901 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     6961 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     7021 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     7081 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     7141 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     7201 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     7261 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     7321 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     7381 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     7441 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     7501 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     7561 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     7621 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     7681 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     7741 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     7801 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     7861 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     7921 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     7981 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     8041 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     8101 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     8161 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     8221 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     8281 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     8341 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     8401 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     8461 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     8521 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     8581 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     8641 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     8701 gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat ggtgcactct
     8761 cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtg
//
