LOCUS       sequence-227215-        5189 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  entry vector for GreenGate cloning method, promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5189)
  AUTHORS   Schurholz AK, Lopez-Salmeron V, Li Z, Forner J, Wenzl C, Gaillochet 
            C, Augustin S, Vilches-Barro A, Fuchs M, Gebert M, Lohmann JU, Greb 
            T, Wolf S
  TITLE     A Comprehensive Toolkit for Inducible, Cell Type-Specific Gene 
            Expression in Arabidopsis.
  JOURNAL   Plant Physiol. 2018 Jul 19. pii: pp.18.00463. doi: 
            10.1104/pp.18.00463.
  PUBMED    30026289
REFERENCE   2  (bases 1 to 5189)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2018 Jul 19. pii: pp.18.00463. doi: 10.1104/pp.18.00463."
COMMENT     Sequence Label: pSW178 - pXPP
FEATURES             Location/Qualifiers
     source          1..5189
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1721..1740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1974..1991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2144..2149,2150..2167,2168..2174)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        2212..2230
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2212..2229
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4775..4794)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4776..4794)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4804..4826)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5020..5039)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5139..5161
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg
     2221 acactataga agtgaagctt ggtctcaacc tggtaccgtg tttggttcgt aattatatac
     2281 ccttccatat tcgaattgga ttcttggtgt tgtatatgtt ttacttttgt actataagtt
     2341 gtaatttccc aatttaaatg ttatgtacta attttatttc gttgtttctt cctttttttc
     2401 cttcggtata tttcgcttat agcagaaaat aacacacata tatatatata acgatataag
     2461 ttataacaca aaatatgatt gtatgtatat caaacaactg aaagagcaaa cacgatttgg
     2521 agactcgtcg ttcagctaaa caaaaaagac atcaaccaaa aacaaagttt atagcatgaa
     2581 gccatgaaca caattcagct ctgttttgtt tcttttttga agaattcgat ttgcattttt
     2641 aggttttgtc atgtgttacg ttcaatatct tagataaaaa catatatgta aaataaaaaa
     2701 tgaaaaacga accaatccgt gatatacata atttagatat gtattatctg cataagaact
     2761 tctgataact taagaacacc tcttacaatt tgggtataaa ctttaaactc aagcattaga
     2821 tttagaagtc gtttacataa tttaactaag caaacgtgtt tgtattagat ccaaagaatt
     2881 aaacagcatg atcgttatta taattagaac tataacttac tattagttga atccagtgac
     2941 taaaataacc ttattttaat ttacaccttt cttagttaaa aaaataaacc aaacactgat
     3001 tcaaaataga aaaataactg tcagagaaga attttctctt taatgacttt tgtattctat
     3061 atgaggattg aacattatgg gttattcata gcaataaaaa cataaccaat gtgaatgtat
     3121 agtttcgatc tttctcgaac aatgatttaa gcacgtgtcg taggttgaat aaataattca
     3181 tagtggaacc atagattagt gacctgtacg agtgattggt ttcagcgtgc agaatccact
     3241 acctcgcttc cactcaattt agtggaccgt cgactctggt gggtttggta gcagcagaag
     3301 ataatgcaca cattacctga atccacattt ttaaaatatg cacttgccag ttctattatc
     3361 ctgaaagttt cgttagaata aaacgcaagc gaaataataa tcctatcaag cattttagat
     3421 aatcactcat tcactctatg acttgtcatt gctgatttgc ggtttgatgc aaaactgtgg
     3481 aatataatga tttaaagaaa tttataatgc gaaaaccttt tcatcgtcgt ggtagaagtg
     3541 aaacctcaat tctttttaca aacgcttttt ttttacctca ccggtggttt aagaaaacct
     3601 tgcgaaataa catggattaa gctaagattt gttcttacct aatttgtaat ttatcacatt
     3661 cacaccccgg attacaattt cattttgcat attaatattg ccaatcaaaa catacattta
     3721 actagactag aaatgtgttt aattaagtat tcgtagttct cttatctaag gtagtatgcg
     3781 taaactatag ggtgaattta atatatatgc tcagaagaat gaaaaataaa gaatctgacc
     3841 atgaagtttt gttatttagt tctctttcat ctgattcaca ctaatgccaa atataccctt
     3901 cgtcacatat acacgttaac aagaaaaata tcaagataaa acaatttata tgcaacaata
     3961 aaaaatgagt ttcggcggta acaagtttag gtagttaatt atttgattag tatgataaac
     4021 atgtctttaa aatatagtag caaggtgaaa gcactattca tcttacgtcg aaaacatgtt
     4081 tctcgtgtta aatcttgtat tatataatat gtattatata ataacatatt tatttatttt
     4141 ggtaatttaa ttattattgt taatttatac attaacaatt tagttatcac cctaacatgt
     4201 cttttaaagt ggtaaattgt atgctactta tagataacta ttatacataa cgaattatta
     4261 ttttttatat catgttaaca ttcagtaact aataaattaa caattatcta ttcttataac
     4321 atattaattt atggattagc ttatttattt atcatgctaa tttgtttatc accggtaatt
     4381 tatgagttat tatatacgtt aaacttcttc ttcttttttt cttctctttc attttcaact
     4441 ctttttctct tacaagggta aaaatgtaaa aacatcactc tctatcttac ctatctaatt
     4501 aagaaaaaaa catgcatata tacttaatta ttcactcttc aatgcatatc cattataatt
     4561 tctctaaact ataataaaac gaaatatgat aaatgtacgt aggttaaatt aattatggtg
     4621 atatacacgt agcggctcac acaatcccaa agcttctata aatacacgaa catgttattc
     4681 atttttgtac ctaatcaagt tacactataa actcaaacac atctatcggc aagtcttaac
     4741 acacgaagat ttccaacatg agaccgaatt ctcgccctat agtgagtcgt attacctggc
     4801 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     4861 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     4921 ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc tccttacgca
     4981 tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc
     5041 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     5101 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     5161 gttttcaccg tcatcaccga aacgcgcga
//
