LOCUS       sequence-227224-        5781 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  miniTn5 plasmid to deliver constitutively expressed fluorescent 
            protein genes in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5781)
  AUTHORS   Schlechter RO, Jun H, Bernach M, Oso S, Boyd E, Munoz-Lintz DA, 
            Dobson RCJ, Remus DM, Remus-Emsermann MNP
  TITLE     Chromatic Bacteria - A Broad Host-Range Plasmid and Chromosomal 
            Insertion Toolbox for Fluorescent Protein Expression in Bacteria.
  JOURNAL   Front Microbiol. 2018 Dec 12;9:3052. doi: 10.3389/fmicb.2018.03052. 
            eCollection 2018.
  PUBMED    30631309
REFERENCE   2  (bases 1 to 5781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol."; date: "2018-12-12"; volume: "9:3052. doi"; pages: " 
            10.3389/fmicb.2018.03052. eCollection 2018"
COMMENT     Sequence Label: pMRE-Tn5-136
FEATURES             Location/Qualifiers
     source          1..5781
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      702..796
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             840..1499
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(906..925)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1564..1650
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(3394..3412)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3480..3584
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3585..3653,3654..4445)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3803..3822)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     oriT            4684..4793
                     /note="incP origin of transfer"
     primer_bind     5347..5363
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5347..5363
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 atggtgagca agggcgagga gctgatcaag gagaacatgc acatgaagct gtacatggaa
       61 ggcaccgtga acaaccacca cttcaagtgc accaccgaag gggagggcaa gccctacgag
      121 ggcacccaga cccagaggat taaggtggtg gagggaggcc ccctgccgtt cgcattcgac
      181 atcctggcca cctgctttat gtacgggagc aagaccttca tcaaccacac ccagggcatc
      241 cccgatttct ttaagcagtc cttccctgag ggcttcacat gggagagagt caccacatac
      301 gaagacgggg gcgtgcttac cgttacccag gacaccagcc tccaggacgg ctgcttgatc
      361 tacaacgtca agctcagagg ggtgaacttc ccatccaacg gccctgtgat gcagaagaaa
      421 acactcggct gggaggccac caccgagacc ctgtaccccg ctgacggcgg cctggaaggc
      481 agatgcgaca tggccctgaa gctcgtgggc gggggccacc tgcactgcaa cctgaagacc
      541 acatacagat ccaagaaacc cgctaagaac ctcaagatgc ccggcgtcta ctttgtggac
      601 cgcagactgg aaagaatcaa ggaggccgac aatgagacct acgtcgagca gcacgaggtg
      661 gctgtggcca gatactgcga cctcctaatt agctgagctt ggactcctgt tgatagatcc
      721 agtaatgacc tcagaactcc atctggattt gttcagaacg ctcggttgcc gccgggcgtt
      781 ttttattggt gagaatccaa gctagcttgg cgagattttc aggagctaag gaagctaaaa
      841 tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac
      901 attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata
      961 ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc
     1021 acattcttgc ccgcctgatg aatgctcatc cggaatttcg tatggcaatg aaagacggtg
     1081 agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa
     1141 cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt
     1201 cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga
     1261 atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg
     1321 ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg
     1381 acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg
     1441 tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaat
     1501 ttttttaagg cagttattgg tgcccttaaa cgcctggggt aatgactctc tagcttgagg
     1561 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
     1621 tcggtgaacg ctctcctgag taggacaaat ccgccgctag gagcttgcgg ccgccaggat
     1681 atcgacccaa gtaccgccac ctaacaattc gttcaagccg agatcggctt cccggccgac
     1741 gcgtcaattc gagcgcgtca attcgagggc cgcggcctag gcggccagat ctgatcaaga
     1801 gacagtctag ctagaattaa ttcgagtcga ccgatcccgt acacaagtag cgtcctgaac
     1861 ggaacctttc ccgttttcca ggatctgatc ttccatgtga cctcctaaca tggtaacgtt
     1921 catgataact tctgctcttc atcgtgcggc cgactgggct aaatctgtgt tctcttcggc
     1981 ggcgctgggt gatcctcgcc gtactgcccg cttggttaac gtcgccgccc aattggcaaa
     2041 atattctggt aaatcaataa ccatctcatc agagggtagt gaagccatgc aggaaggcgc
     2101 ttaccgattt atccgcaatc ccaacgtttc tgccgaggcg atcagaaagg ctggcgccat
     2161 gcaaacagtc aagttggctc aggagtttcc cgaactgctg gccattgagg acaccacctc
     2221 tttgagttat cgccaccagg tcgccgaaga gcttggcaag ctgggctcta ttcaggataa
     2281 atcccgcgga tggtgggttc actccgttct cttgctcgag gccaccacat tccgcaccgt
     2341 aggattactg catcaggagt ggtggatgcg cccggatgac cctgccgatg cggatgaaaa
     2401 ggagagtggc aaatggctgg cagccgccgc aactagccgg ttacgcatgg gcagcatgat
     2461 gagcaacgtg attgcggtct gtgaccgcga agccgatatt catgcttatc tgcaggacaa
     2521 actggcgcat aacgagcgct tcgtggtgcg ctccaagcac ccacgcaagg acgtagagtc
     2581 tgggttgtat ctgtacgacc atctgaagaa ccaaccggag ttgggtggct atcagatcag
     2641 cattccgcaa aagggcgtgg tggataaacg cggtaaacgt aaaaatcgac cagcccgcaa
     2701 ggcgagcttg agcctgcgca gtgggcgcat cacgctaaaa caggggaata tcacgctcaa
     2761 cgcggtgctg gccgaggaga ttaacccgcc caagggtgag accccgttga aatggttgtt
     2821 gctgaccagc gaaccggtcg agtcgctagc ccaagccttg cgcgtcatcg acatttatac
     2881 ccatcgctgg cggatcgagg agttccataa ggcatggaaa accggagcag gagccgagag
     2941 gcaacgcatg gaggagccgg ataatctgga gcggatggtc tcgatcctct cgtttgttgc
     3001 ggtcaggctg ttacagctca gagaaagctt cacgctgccg caagcactca gggcgcaagg
     3061 gctgctaaag gaagcggaac acgtagaaag ccagtccgca gaaacggtgc tgaccccgga
     3121 tgaatgtcag ctactgggct atctggacaa gggaaaacgc aagcgcaaag agaaagcagg
     3181 tagcttgcag tgggcttaca tggcgatagc tagactgggc ggttttatgg acagcaagcg
     3241 aaccggaatt gccagctggg gcgccctctg ggaaggttgg gaagccctgc aaagtaaact
     3301 ggatggcttt cttgccgcca aggatctgat ggcgcagggg atcaagatct gatcggtcga
     3361 tcgaattaat tcccaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg
     3421 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     3481 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     3541 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     3601 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     3661 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     3721 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     3781 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     3841 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     3901 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     3961 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     4021 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     4081 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     4141 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     4201 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     4261 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     4321 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     4381 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     4441 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     4501 aatttaaaag gatctaggtg aagatccggg gaattgggga tcaattccgt tttccgctgc
     4561 ataaccctgc ttcggggtca ttatagcgat tttttcggta tatccatcct ttttcgcacg
     4621 atatacagga ttttgccaaa gggttcgtgt agactttcct tggtgtatcc aacggcgtca
     4681 gccgggcagg ataggtgaag taggcccacc cgcgagcggg tgttccttct tcactgtccc
     4741 ttattcgcac ctggcggtgc tcaacgggaa tcctgctctg cgaggctggc cggctaccgc
     4801 cggcgtaaca gatgagggca agcggatggc tgatgaaacc aagccaacca ggaagggcag
     4861 cccacctatc acggaattga tccggccacg atgcgtccgg cgtagaggat ctgaagatca
     4921 gcagttcaac ctgttgatag tacgtactaa gctctcatgt ttcacgtact aagctctcat
     4981 gtttaacgta ctaagctctc atgtttaacg aactaaaccc tcatggctaa cgtactaagc
     5041 tctcatgttt cacgtactaa gctctcatgt ttgaacaata aaattaatat aaatcagcaa
     5101 cttaaatagc ctctaaggtt ttaagtttta taagaaaaaa aagaatatat aaggctttta
     5161 aagcttttaa ggtttaacgg ttgtggacaa caagccaggg atgtaacgca ctgagaagcc
     5221 cttagagcct ctcaaagcaa ttttgagtga cacaggaaca cttaacggct gacatgggaa
     5281 ttaattctga ctcttataca caagtgcggc cctcgaattg acgcgctcca ccgcggtggc
     5341 ggccgctcta gaactagtgg atcccccggg ctgcaggaat tcgatatcca taaactgcca
     5401 ggaattgggg atcggattcg agctcgcacg ctgccgcaag cactcagggc gcaagggctg
     5461 ctaaaggaag cggaacacgt agaaagccag tccgcagaaa cggtgctgac cccggatgaa
     5521 tgtcagctac tgggctatct ggacaaggga aaacgcaagc gcaaagagaa agcaggtagc
     5581 ttgcagtggg cttacatggc gatagctaga ctgggcggtt ttatggacag caagcgaacc
     5641 ggaattgcca gctggggcgc cctctggtaa ggttgggaat ccctgcaaag taaactggat
     5701 ggctttcttg ccgccaagga tctgatggcg caggggatca agatctgatc aagagacagg
     5761 atactagtgg aggaagaaaa a
//
