LOCUS       sequence-227227-        7200 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry clone containing CAS9p-TagRFP and 35S terminator. Used to make
            CRISPR construct . For use in plants and compatible with the 
            MultiSite Gateway system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7200)
  AUTHORS   Wang X, Ye L, Lyu M, Ursache R, Loytynoja A, Mahonen AP
  TITLE     An inducible genome editing system for plants.
  JOURNAL   Nat Plants. 2020 Jun 29. pii: 10.1038/s41477-020-0695-2. doi: 
            10.1038/s41477-020-0695-2.
  PUBMED    32601420
REFERENCE   2  (bases 1 to 7200)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Plants.
            2020 Jun 29. pii: 10.1038/s41477-020-0695-2. doi: 
            10.1038/s41477-020-0695-2."
COMMENT     Sequence Label: p221z-CAS9p-TagRFP-t35s
FEATURES             Location/Qualifiers
     source          1..7200
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     65..82
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(241..268)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(360..446)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     495..517
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     509..526
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     510..526
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    542..641
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             650..670
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             695..4795
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             695..4795
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             1881..1889
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             4796..5485
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSDMALK
                     LVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAVARYC
                     DLPSKLGH"
     CDS             5489..5536
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     protein_bind    complement(5769..5868)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(5885..5904)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5886..5904)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5909..5925)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5909..5925)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             complement(5993..6367)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(6386..6433)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     rep_origin      6523..7111
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7012..7031
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
       61 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
      121 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
      181 cagtgagcgc aacgcaatta atacgcgtac cgctagccag gaagagtttg tagaaacgca
      241 aaaaggccat ccgtcaggat ggccttctgc ttagtttgat gcctggcagt ttatggcggg
      301 cgtcctgccc gccaccctcc gggccgttgc ttcacaacgt tcaaatccgc tcccggcgga
      361 tttgtcctac tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct
      421 tccgactgag cctttcgttt tatttgatgc ctggcagttc cctactctcg cgttaacgct
      481 agcatggatg ttttcccagt cacgacgttg taaaacgacg gccagtctta agctcgggcc
      541 ccaaataatg attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgatgagca
      601 atgctttttt ataatgccaa ctttgtacaa aaaagcaggc tcgatggctc ctaagaagaa
      661 gcggaaggtt ggtattcacg gggtgcctgc ggctgacaag aagtactcca tcggcctcga
      721 catcggcacc aacagcgtcg gctgggcggt gatcaccgac gagtacaagg tcccgtccaa
      781 gaagttcaag gtcctgggca acaccgaccg ccactccatc aagaagaacc tcatcggcgc
      841 cctcctcttc gactccggcg agacggcgga ggcgacccgc ctcaagcgca ccgcccgccg
      901 ccgctacacc cgccgcaaga accgcatctg ctacctccag gagatcttct ccaacgagat
      961 ggcgaaggtc gacgactcct tcttccaccg cctcgaggag tccttcctcg tggaggagga
     1021 caagaagcac gagcgccacc ccatcttcgg caacatcgtc gacgaggtcg cctaccacga
     1081 gaagtacccc actatctacc accttcgtaa gaagcttgtt gactctactg ataaggctga
     1141 tcttcgtctc atctaccttg ctctcgctca catgatcaag ttccgtggtc acttccttat
     1201 cgagggtgac cttaaccctg ataactccga cgtggacaag ctcttcatcc agctcgtcca
     1261 gacctacaac cagctcttcg aggagaaccc tatcaacgct tccggtgtcg acgctaaggc
     1321 gatcctttcc gctaggctct ccaagtccag gcgtctcgag aacctcatcg cccagctccc
     1381 tggtgagaag aagaacggtc ttttcggtaa cctcatcgct ctctccctcg gtctgacccc
     1441 taacttcaag tccaacttcg acctcgctga ggacgctaag cttcagctct ccaaggatac
     1501 ctacgacgat gatctcgaca acctcctcgc tcagattgga gatcagtacg ctgatctctt
     1561 ccttgctgct aagaacctct ccgatgctat cctcctttcg gatatcctta gggttaacac
     1621 tgagatcact aaggctcctc tttctgcttc catgatcaag cgctacgacg agcaccacca
     1681 ggacctcacc ctcctcaagg ctcttgttcg tcagcagctc cccgagaagt acaaggagat
     1741 cttcttcgac cagtccaaga acggctacgc cggttacatt gacggtggag ctagccagga
     1801 ggagttctac aagttcatca agccaatcct tgagaagatg gatggtactg aggagcttct
     1861 cgttaagctt aaccgtgagg acctccttag gaagcagagg actttcgata acggctctat
     1921 ccctcaccag atccaccttg gtgagcttca cgccatcctt cgtaggcagg aggacttcta
     1981 ccctttcctc aaggacaacc gtgagaagat cgagaagatc cttactttcc gtattcctta
     2041 ctacgttggt cctcttgctc gtggtaactc ccgtttcgct tggatgacta ggaagtccga
     2101 ggagactatc accccttgga acttcgagga ggttgttgac aagggtgctt ccgcccagtc
     2161 cttcatcgag cgcatgacca acttcgacaa gaacctcccc aacgagaagg tcctccccaa
     2221 gcactccctc ctctacgagt acttcacggt ctacaacgag ctcaccaagg tcaagtacgt
     2281 caccgagggt atgcgcaagc ctgccttcct ctccggcgag cagaagaagg ctatcgttga
     2341 cctcctcttc aagaccaacc gcaaggtcac cgtcaagcag ctcaaggagg actacttcaa
     2401 gaagatcgag tgcttcgact ccgtcgagat cagcggcgtt gaggaccgtt tcaacgcttc
     2461 tctcggtacc taccacgatc tcctcaagat catcaaggac aaggacttcc tcgacaacga
     2521 ggagaacgag gacatcctcg aggacatcgt cctcactctt actctcttcg aggataggga
     2581 gatgatcgag gagaggctca agacttacgc tcatctcttc gatgacaagg ttatgaagca
     2641 gctcaagcgt cgccgttaca ccggttgggg taggctctcc cgcaagctca tcaacggtat
     2701 cagggataag cagagcggca agactatcct cgacttcctc aagtctgatg gtttcgctaa
     2761 caggaacttc atgcagctca tccacgatga ctctcttacc ttcaaggagg atattcagaa
     2821 ggctcaggtg tccggtcagg gcgactctct ccacgagcac attgctaacc ttgctggttc
     2881 ccctgctatc aagaagggca tccttcagac tgttaaggtt gtcgatgagc ttgtcaaggt
     2941 tatgggtcgt cacaagcctg agaacatcgt catcgagatg gctcgtgaga accagactac
     3001 ccagaagggt cagaagaact cgagggagcg catgaagagg attgaggagg gtatcaagga
     3061 gcttggttct cagatcctta aggagcaccc tgtcgagaac acccagctcc agaacgagaa
     3121 gctctacctc tactacctcc agaacggtag ggatatgtac gttgaccagg agctcgacat
     3181 caacaggctt tctgactacg acgtcgacca cattgttcct cagtctttcc ttaaggatga
     3241 ctccatcgac aacaaggtcc tcacgaggtc cgacaagaac aggggtaagt cggacaacgt
     3301 cccttccgag gaggttgtca agaagatgaa gaactactgg aggcagcttc tcaacgctaa
     3361 gctcattacc cagaggaagt tcgacaacct cacgaaggct gagaggggtg gcctttccga
     3421 gcttgacaag gctggtttca tcaagaggca gcttgttgag acgaggcaga ttaccaagca
     3481 cgttgctcag atcctcgatt ctaggatgaa caccaagtac gacgagaacg acaagctcat
     3541 ccgcgaggtc aaggtgatca ccctcaagtc caagctcgtc tccgacttcc gcaaggactt
     3601 ccagttctac aaggtccgcg agatcaacaa ctaccaccac gctcacgatg cttaccttaa
     3661 cgctgtcgtt ggtaccgctc ttatcaagaa gtaccctaag cttgagtccg agttcgtcta
     3721 cggtgactac aaggtctacg acgttcgtaa gatgatcgcc aagtccgagc aggagatcgg
     3781 caaggccacc gccaagtact tcttctactc caacatcatg aacttcttca agaccgagat
     3841 caccctcgcc aacggcgaga tccgcaagcg ccctcttatc gagacgaacg gtgagactgg
     3901 tgagatcgtt tgggacaagg gtcgcgactt cgctactgtt cgcaaggtcc tttctatgcc
     3961 tcaggttaac atcgtcaaga agaccgaggt ccagaccggt ggcttctcca aggagtctat
     4021 ccttccaaag agaaactcgg acaagctcat cgctaggaag aaggattggg accctaagaa
     4081 gtacggtggt ttcgactccc ctactgtcgc ctactccgtc ctcgtggtcg ccaaggtgga
     4141 gaagggtaag tcgaagaagc tcaagtccgt caaggagctc ctcggcatca ccatcatgga
     4201 gcgctcctcc ttcgagaaga acccgatcga cttcctcgag gccaagggct acaaggaggt
     4261 caagaaggac ctcatcatca agctccccaa gtactctctt ttcgagctcg agaacggtcg
     4321 taagaggatg ctggcttccg ctggtgagct ccagaagggt aacgagcttg ctcttccttc
     4381 caagtacgtg aacttcctct acctcgcctc ccactacgag aagctcaagg gttcccctga
     4441 ggataacgag cagaagcagc tcttcgtgga gcagcacaag cactacctcg acgagatcat
     4501 cgagcagatc tccgagttct ccaagcgcgt catcctcgct gacgctaacc tcgacaaggt
     4561 cctctccgcc tacaacaagc accgcgacaa gcccatccgc gagcaggccg agaacatcat
     4621 ccacctcttc acgctcacga acctcggcgc ccctgctgct ttcaagtact tcgacaccac
     4681 catcgacagg aagcgttaca cgtccaccaa ggaggttctc gacgctactc tcatccacca
     4741 gtccatcacc ggtctttacg agactcgtat cgacctttcc cagcttggtg gtgatatgag
     4801 cgagctgatt aaggagaaca tgcacatgaa gctgtacatg gagggcaccg tgaacaacca
     4861 ccacttcaag tgcacatccg agggcgaagg caagccctac gagggcaccc agaccatgag
     4921 aatcaaggtg gtcgagggcg gccctctccc cttcgccttc gacatcctgg ctaccagctt
     4981 catgtacggc agcagaacct tcatcaacca cacccagggc atccccgact tctttaagca
     5041 gtccttccct gagggcttca catgggagag agtcaccaca tacgaagacg ggggcgtgct
     5101 gaccgctacc caggacacca gcctccagga cggctgcctc atctacaacg tcaagatcag
     5161 aggggtgaac ttcccatcca acggccctgt gatgcagaag aaaacactcg gctgggaggc
     5221 caacaccgag atgctgtacc ccgctgacgg cggcctggaa ggcagaagcg acatggccct
     5281 gaagctcgtg ggcgggggcc acctgatctg caacttcaag accacataca gatccaagaa
     5341 acccgctaag aacctcaaga tgcccggcgt ctactatgtg gaccacagac tggaaagaat
     5401 caaggaggcc gacaaagaga cctacgtcga gcagcacgag gtggctgtgg ccagatactg
     5461 cgacctccct agcaaactgg ggcacaagaa gcgtcctgct gccaccaaaa aggccggaca
     5521 ggctaagaaa aagaagtagg atccacctga tctagagtcc gcaaaaatca ccagtctctc
     5581 tctacaaatc tatctctctc tatttttctc cagaataatg tgtgagtagt tcccagataa
     5641 gggaattagg gttcttatag ggtttcgctc atgtgttgag catataagaa acccttagta
     5701 tgtatttgta tttgtaaaat acttctatca ataaaatttc taattcctaa aaccaaaatc
     5761 cagtgaccac ccagctttct tgtacaaagt tggcattata agaaagcatt gcttatcaat
     5821 ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgcc atccagctga
     5881 tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt
     5941 gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat gatcagtcct
     6001 gctcctcggc cacgaagtgc acgcagttgc cggccgggtc gcgcagggcg aactcccgcc
     6061 cccacggctg ctcgccgatc tcggtcatgg ccggcccgga ggcgtcccgg aagttcgtgg
     6121 acacgacctc cgaccactcg gcgtacagct cgtccaggcc gcgcacccac acccaggcca
     6181 gggtgttgtc cggcaccacc tggtcctgga ccgcgctgat gaacagggtc acgtcgtccc
     6241 ggaccacacc ggcgaagtcg tcctccacga agtcccggga gaacccgagc cggtcggtcc
     6301 agaactcgac cgctccggcg acgtcgcgcg cggtgagcac cggaacggca ctggtcaact
     6361 tggccatggt ttagttcctc accttgtcgt attatactat gccgatatac tatgccgatg
     6421 attaattgtc aacacgtgct gatcatgacc aaaatccctt aacgtgagtt acgcgtcgtt
     6481 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     6541 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     6601 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     6661 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     6721 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     6781 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     6841 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     6901 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     6961 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     7021 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     7081 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     7141 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
//
