LOCUS       sequence-227234-        4423 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses araC constitutively and alpha-hemolysin under a pBAD 
            promoter; alternate name pSB1A2-AD009.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4423)
  AUTHORS   Dupin A, Simmel FC
  TITLE     Signalling and differentiation in emulsion-based 
            multi-compartmentalized in vitro gene circuits.
  JOURNAL   Nat Chem. 2018 Nov 26. pii: 10.1038/s41557-018-0174-9. doi: 
            10.1038/s41557-018-0174-9.
  PUBMED    30478365
REFERENCE   2  (bases 1 to 4423)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem. 
            2018 Nov 26. pii: 10.1038/s41557-018-0174-9. doi: 
            10.1038/s41557-018-0174-9."
COMMENT     Sequence Label: pSB1A3-AD009
FEATURES             Location/Qualifiers
     source          1..4423
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(192..296)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     364..382
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(386..429)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    432..453
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     CDS             complement(539..1417)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1444..1728
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1651..1670
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     misc_feature    2798..2818
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(2824..2841)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2995..3583)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3075..3094)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3754..4423,1..122,123..191))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4377..4396
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
       61 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      121 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
      181 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
      241 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
      301 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
      361 aaaaataggc gtatcacgag gcagaatttc agataaaaaa aatccttagc tttcgctaag
      421 gatgatttct ggaattcgcg gccgcttcta gaggagagtt aggcataagg ctgcatgcta
      481 ccctttggtc gaaaaaaaaa gcccgcactg tcaggtgcgg gcttttttct gtgtttcctt
      541 atgacaactt gacggctaca tcattcactt tttcttcaca accggcacgg aactcgctcg
      601 ggctggcccc ggtgcatttt ttaaataccc gcgagaaata gagttgatcg tcaaaaccaa
      661 cattgcgacc gacggtggcg ataggcatcc gggtggtgct caaaagcagc ttcgcctggc
      721 tgatacgttg gtcctcgcgc cagcttaaga cgctaatccc taactgctgg cggaaaagat
      781 gtgacagacg cgacggcgac aagcaaacat gctgtgcgac gctggcgata tcaaaattgc
      841 tgtctgccag gtgatcgctg atgtactgac aagcctcgcg tacccgatta tccatcggtg
      901 gatggagcga ctcgttaatc gcttccatgc gccgcagtaa caattgctca agcagattta
      961 tcgccagcag ctccgaatag cgcccttccc cttgcccggc gttaatgatt tgcccaaaca
     1021 ggtcgctgaa atgcggctgg tgcgcttcat ccgggcgaaa gaaccccgta ttggcaaata
     1081 ttgacggcca gttaagccat tcatgccagt aggcgcgcgg acgaaagtaa acccactggt
     1141 gataccattc gcgagcctcc ggatgacgac cgtagtgatg aatctctcct ggcgggaaca
     1201 gcaaaatatc acccggtcgg caaacaaatt ctcgtccctg atttttcacc accccctgac
     1261 cgcgaatggt gagattgaga atataacctt tcattcccag cggtcggtcg ataaaaaaat
     1321 cgagataacc gttggcctca atcggcgtta aacccgccac cagatgggca ttaaacgagt
     1381 atcccggcag caggggatca ttttgcgctt cagccatact tttcatactc ccgccattca
     1441 gagaagaaac caattgtcca tattgcatca gacattgccg tcactgcgtc ttttactggc
     1501 tcttctcgct aaccaaaccg gtaaccccgc ttattaaaag cattctgtaa caaagcggga
     1561 ccaaagccat gacaaaaacg cgtaacaaaa gtgtctataa tcacggcaga aaagtccaca
     1621 ttgattattt gcacggcgtc acactttgct atgccatagc atttttatcc ataagattag
     1681 cggatcctac ctgacgcttt ttatcgcaac tctctactgt ttctccatac ccgttttttt
     1741 gggctagcga attcgagctc ggtacccggg gatcctctag aaggagatat aaccctatgg
     1801 actatgtttt tcaaagacga aaaactacta gatggattct gatatcaata tcaaaaccgg
     1861 caccaccgat atcggctcca ataccaccgt taaaaccggt gatctggtga cctatgataa
     1921 agaaaacggt atgcataaaa aagtgtttta ctcgtttatt gacgataaaa accataacaa
     1981 aaaactgctg gtcatccgca ccaaaggcac cattgcgggt caataccgtg tgtactccga
     2041 agaaggtgcg aacaaaagcg gtctggcttg gccgtctgcc tttaaagtgc agctgcaact
     2101 gccggataat gaagtggcgc agatttcaga ttattatccg cgtaatagca tcgataccaa
     2161 agaatatatg agtaccctga cctatggttt taatggcaat gttaccggtg atgatacggg
     2221 taaaattggc ggtctgattg gcgccaatgt gtccattggt catacgctga aatacgtgca
     2281 accggatttc aaaaccattc tggaaagtcc gaccgataaa aaagtgggtt ggaaagttat
     2341 cttcaacaac atggtgaatc agaactgggg tccgtacgat cgcgattcct ggaatccggt
     2401 ttatggcaat cagctgttta tgaaaacccg caacggtagt atgaaagcgg cggataattt
     2461 tctggacccg aacaaagcct caagcctgct gtccagcggt tttagcccgg attttgccac
     2521 ggttattacc atggatcgca aagccagcaa acagcagacc aacattgatg tgatctacga
     2581 acgtgtgcgt gatgattatc aactgcattg gacctcaacc aattggaaag gcaccaatac
     2641 caaagataaa tggacggatc gcagttcaga acgctacaaa attgattggg aaaaagaaga
     2701 aatgaccaac taatagaacg catgagaaag cccccggaag atcaccttcc gggggctttt
     2761 ttattgcgcc ttgtcacacc tacactgctc gaagtactac tagtagcggc cgctgcaggc
     2821 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     2881 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     2941 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     3001 caggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     3061 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     3121 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     3181 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     3241 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     3301 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     3361 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     3421 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     3481 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     3541 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     3601 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcattag
     3661 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     3721 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     3781 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     3841 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     3901 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     3961 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     4021 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     4081 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     4141 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     4201 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     4261 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     4321 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     4381 taccgcgcca catagcagaa ctttaaaagt gctcatcatt gga
//
