LOCUS       sequence-227245-        4813 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  entry vector for GreenGate cloning method, promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4813)
  AUTHORS   Schurholz AK, Lopez-Salmeron V, Li Z, Forner J, Wenzl C, Gaillochet 
            C, Augustin S, Vilches-Barro A, Fuchs M, Gebert M, Lohmann JU, Greb 
            T, Wolf S
  TITLE     A Comprehensive Toolkit for Inducible, Cell Type-Specific Gene 
            Expression in Arabidopsis.
  JOURNAL   Plant Physiol. 2018 Jul 19. pii: pp.18.00463. doi: 
            10.1104/pp.18.00463.
  PUBMED    30026289
REFERENCE   2  (bases 1 to 4813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2018 Jul 19. pii: pp.18.00463. doi: 10.1104/pp.18.00463."
COMMENT     Sequence Label: pSW614 - pREV
FEATURES             Location/Qualifiers
     source          1..4813
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1721..1740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1974..1991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2144..2149,2150..2167,2168..2174)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        2212..2230
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2212..2229
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4399..4418)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4400..4418)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4428..4450)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4644..4663)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4763..4785
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg
     2221 acactataga agtgaagctt ggtctcaacc tacacctctt tctgattact agcccactct
     2281 ttggtggttc ttacatatca cacctaacta tactgtgtat ccttgaagtg aaaatcaaat
     2341 ttaccattcg tatcttactt acatacacta ttatttttcc tttttttttt cactcaaggt
     2401 cctactcttt gataccatag ctataatttg gaaataacta tttacagtgt attaattata
     2461 cctaaacagt ttaatctgga ctaaatattt agatagatgt tacaaatttg gttcgtctaa
     2521 taaatgaaga caagacatgc taacaaataa aacactacca caaagggata gtgagagaat
     2581 gtgttttgca acaagacata actttgattg cttgatgtgt taaaatgatt atgtcagaga
     2641 cagagagcga tcataggctt tctttatttc taataacgtc gattttcttc ttcttttctg
     2701 gcgttcaata atgtcgaatt ttaattcttg atttttgcaa ctctaaatat ccttaacgac
     2761 attgaagcat ggtgcatgtc gatcgttaat aataaagttg aacaaaaatc ttgttgaatt
     2821 aattacaagc acagcttcaa tagcataact ttacgagacg accagatctt atagacgagt
     2881 ttcgctttta cttttttaat gattaaaact ttcatcggag aacataagtc ttcctcttaa
     2941 ttaaaattac tacccgtgca taacttcatt ttttaaaaca tcaataatta atatacgatt
     3001 acaaccctaa aaattagtca ccctatagta cataacaatg atgatagttt tttctttttg
     3061 gtgtaatgtt aaaataaaat gttagccata ttaaacgata gttcttttaa cttagccatt
     3121 gtaagatatt tcttacttta gtttttccgt agaagatatt cattatggta tggatagtat
     3181 ataccttaac tgagtttaaa tattggatca ataccatcta ataacacata tctgatgttc
     3241 aatacttaat aattttgcat aaatgttaag cgtgacaact taaaaaaaaa cacatcaaca
     3301 gagtaaaaac atatctgtta aataagaaaa atgtcatttt tataacactt aaaaaaaaat
     3361 gcatgaagcg tttcatagtt tttttttttt tcaaagtaat gtaggcgtta gatatttctt
     3421 acaatttttt gaaaaatatt ttttatgttg tgattggctg atatcaggta actaaaactt
     3481 ctttaaagaa ttgaagaaaa tttgaaaagt aaatagatgg attcctatat tgtcatttca
     3541 gaaaaacagt agggacaact tcgtaaatga tagccgtatt attaaacaaa taaatttaaa
     3601 ttagaaaaaa ggaaaaaaac gcaccacttt tctttttcgc tgatgcacag cttgtcggtt
     3661 tgcgtgcaaa tcctctgttt cacaattttt tcttcttctc tttctctctc ttcctctttt
     3721 attcctctgt tccaaagttc agcagaagca aacacacaca tcacttacta tctctctctc
     3781 cttcttcact ttctcacata accaaactct ctctttctct cttttttttg aagtctcctt
     3841 tgaaactata attgcccttt agtgttgttc gttcagagtc ttcaaaactt ttgcagcttc
     3901 aattgtacct gggtttcttc ttcattgttc ctaaggtttc tgtgtccttc aattcttctg
     3961 atataatgct tctttaagag agttgacatc atcactttct tggggtactc ttctctgttt
     4021 ctccccagaa aatccaactc tgtaattttg ggtctttatt ctgtttttct ctttgaagaa
     4081 tctttaaaat tctcagatct tctgaatctc tcttctttaa aacttttttt aactttattt
     4141 tttgtactcg cttctttgcc ttcatttttc tcgtatccac atgtcgttgg tctttcgcta
     4201 caagccacga ccgtagaatc ttcttttgtc tgaaaagaat tacaatttac gtttctctta
     4261 cgatacgacg gactttccga agaaattaat ttaaagagaa aagaagaaga agccaaagaa
     4321 gaagaagaag ctagaagaaa cagtaaagtt tgagactttt tttgagggtc gagctaaaaa
     4381 catgagaccg aattctcgcc ctatagtgag tcgtattacc tggccgtcgt tttacaacgt
     4441 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     4501 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     4561 ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     4621 caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
     4681 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     4741 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     4801 ccgaaacgcg cga
//
