LOCUS       sequence-227257-        1999 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression of Che9c60 and Che9c61 recombinases on 
            pB264/pBR322 backbone with kanamycin resistance..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   DeLorenzo DM, Rottinghaus AG, Henson WR, Moon TS
  TITLE     Molecular Toolkit for Gene Expression Control and Genome 
            Modification in Rhodococcus opacus PD630.
  JOURNAL   ACS Synth Biol. 2018 Feb 16;7(2):727-738. doi: 
            10.1021/acssynbio.7b00416. Epub 2018 Feb 5.
  PUBMED    29366319
REFERENCE   2  (bases 1 to 1999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.7b00416"; journalName: "ACS Synth Biol"; date: 
            "2018-02-16- 16"; volume: "7"; issue: "2"; pages: "727-738"
COMMENT     Sequence Label: pDD120
FEATURES             Location/Qualifiers
     source          1..1999
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgagtgtgc ccacacagga cggaatgcac cggttcgtcg acgaggacgt ctaccacgct
       61 gaccggggct cgctgtcggt atccggcgcg aagctgctgt tgccgccgtc gtgtcccgcg
      121 aaattccgct gggagatgga caacacccgg aagccgaaaa aggtctggga cttcggacat
      181 gtcgcgcaca aactggtgct cggcaagggt gccgagttcg agatcctcga ccccgaggtg
      241 cacgggctga aggcggacgg tacgccgtcg gagaagccga ccgcgacggg catgtggcgc
      301 aaggccgagg ctgaggctcg caaacagggc aaggtgccga ttcacgtcga cctgttcacg
      361 aaggcgtacg acatggccga aaaggtgcgt cagcacccga cagccggccc gatcttcgcc
      421 aatcctgacg gcgaggccga ggtcgcgctg tactacaccg accccgagac cggcgtgcgg
      481 ctgcgtggcc ggatcgactg gctcactgac gatatcgatg attacaagac gtcgatgacc
      541 gcgaacccgg ccgagctgaa aaccaagttc tacaagctcg gctatttcat gcaggcggcc
      601 tggtacatcg atctactggt cgccctcggg ctcgccgaga acccgcgatt ccggttcatc
      661 acgcaggaga aagaaccgcc ctacgtcgtg actccgatcc agtacgacga cgaggcgatc
      721 gaagaggggc ggcgccgcaa ccgccaggcg atccggctct acgccgactg catggaatcg
      781 ggcaagtggc ctgactacag cgacgacgtg gtcacgatca gcctgccctc gtgggggctg
      841 ccgcgaccgc agaccgtcgg cgacgtcgtc accgacagct atatctacga caccgacccg
      901 ctcgaagagg ccgacccgat tgaaggggat tacatctatg gctgaaaatg ctgtcaccaa
      961 gcaggattcg cccaaggcac ccgagacgat ctcgcaagtg ctgcaggtgc tcgtgccgca
     1021 gctggcgcgt gcagtgccca agggtatgga ccccgaccgc atcgcgcgga tcgtgcaaac
     1081 cgagatccgc aagtcgcgca acgcgaaagc tgctggcatc gctaagcagt ccctcgacga
     1141 ctgcacgcaa gagtcatttg ccggtgcgct gctgacctcg gccgcgctcg gtctcgagcc
     1201 cggtgtcaac ggcgagtgct acctcgtgcc ctaccgcgac acccggcgcg gcgtggtcga
     1261 gtgccagctg atcatcggct accagggcat cgtcaaactg ttctggcagc acccgcgcgc
     1321 ctcccggatc gacgcgcagt gggtcggcgc gaacgacgaa ttccattaca ccatgggcct
     1381 caatccgacg ctgaaacacg tgaaggccaa gggtgatcgg ggtaatccgg tctacttcta
     1441 cgcgatcgtc gaggtgaccg gcgctgagcc gctgtgggac gtgttcaccg ccgacgagat
     1501 cagggaattg cgtcgcggca aggtcggatc ctcgggcgac atcaaggacc cgcagcgctg
     1561 gatggagcgg aagaccgcgc tcaaacaggt gctgaagctg gcaccgaaga cgacgcggct
     1621 cgacgcggcg atccgcgccg acgatcgccc gggcaccgac ctgtcacagt cgcaggcgct
     1681 cgcgctgccg tcgaccgtca agccgacggc cgactacatc gacggcgaga tcgccgagcc
     1741 gcacgaggtc gatacgccac cgaagtcgtc gcgcgcacaa cgcgcgcagc gcgccaccgc
     1801 gccggcgccc gacgtgcaga tggccaatcc cgatcagctg aagcgcctcg gcgagatcca
     1861 gaaggccgaa aagtacaacg acgccgattg gttcaagttc ctcgccgatt cggccggcgt
     1921 caaagccacg cgcgccgccg acctcacgtt cgacgaggcg aaggctgtca tcgacatgtt
     1981 cgacgggccc aacgcatga
//
