LOCUS       sequence-227260-         436 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Integration vector into the ROCI-3 site of Rhodococcus opacus PD630 
            containing constitutive EYFP and hygromycin B resistance marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 436)
  AUTHORS   DeLorenzo DM, Rottinghaus AG, Henson WR, Moon TS
  TITLE     Molecular Toolkit for Gene Expression Control and Genome 
            Modification in Rhodococcus opacus PD630.
  JOURNAL   ACS Synth Biol. 2018 Feb 16;7(2):727-738. doi: 
            10.1021/acssynbio.7b00416. Epub 2018 Feb 5.
  PUBMED    29366319
REFERENCE   2  (bases 1 to 436)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.7b00416"; journalName: "ACS Synth Biol"; date: 
            "2018-02-16- 16"; volume: "7"; issue: "2"; pages: "727-738"
COMMENT     Sequence Label: pDD132
FEATURES             Location/Qualifiers
     source          1..436
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cgccttgtcg tggatcttct ttccggcggc ggtcagtccg gcggcgtggt gttcgccttt
       61 tcccacgtcg atgccgcaaa acacggcgta actcgtgtcc attcagcagt ccttcgtccg
      121 gccagtccgg tcgccgatcc agcatcgata cccgacaccc acgttacgag gtgacctacc
      181 gcagtttccg gtggtcctgt ccctatcagc ggtccatcga cgccaccagg ttcggcgaca
      241 ccacccccgg atcattcgag agacaggggc aaagaagtca taccgaagat ggcgaccgca
      301 gtcccgcgaa acgggaccac aaagacggta acggcaaacc ggcctgggaa cccgcgggtt
      361 tcgccaccct cgaacgggtc ctggcgacga gctgaccgcg tcgcggcgcc cgataagaga
      421 ccactcgagc tccgac
//
