LOCUS       sequence-227261-         498 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Integration vector into the ROCI-3 site of Rhodococcus opacus PD630 
            containing constitutive EYFP and hygromycin B resistance marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 498)
  AUTHORS   DeLorenzo DM, Rottinghaus AG, Henson WR, Moon TS
  TITLE     Molecular Toolkit for Gene Expression Control and Genome 
            Modification in Rhodococcus opacus PD630.
  JOURNAL   ACS Synth Biol. 2018 Feb 16;7(2):727-738. doi: 
            10.1021/acssynbio.7b00416. Epub 2018 Feb 5.
  PUBMED    29366319
REFERENCE   2  (bases 1 to 498)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.7b00416"; journalName: "ACS Synth Biol"; date: 
            "2018-02-16- 16"; volume: "7"; issue: "2"; pages: "727-738"
COMMENT     Sequence Label: pDD132
FEATURES             Location/Qualifiers
     source          1..498
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ttgggtcgga tagtgtcgag aactgcgcgg tcatggtatt cgggacgttt gagtgccgag
       61 caagcggctc gcatcactcg gtggcttccc ggtgcccgcc tgtgtgctga catgctttcc
      121 agtgctcgag tttgtcgcca aacatcgagc cggagatgaa gaacagatca tgtaattcaa
      181 tctcttcgcc gagacgggtg acgaggcgat tgacctcgtc acgaggtact tcgtcaacga
      241 gtttcgatga gctgtgatag tgctgccagg tgctgcgcat ctcgtcgagc ccatacgtca
      301 cttcggtcgg gtcgttcagc ccggtccttg cagttgccca caagcagtgg ttctccacga
      361 tccacgctca gccgccgacg ccgccgtgca gcgttggcat acgtgcttcg tgcagtagct
      421 gttcgtccgt gtctgccggc cactcggtgg tgccgccgac gcgggaccat tgcgcgatca
      481 ggcatgagaa ggtgctct
//
