LOCUS       sequence-227263-         495 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Integration vector into the ROCI-2 site of Rhodococcus opacus PD630 
            containing constitutive EYFP and hygromycin B resistance marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 495)
  AUTHORS   DeLorenzo DM, Rottinghaus AG, Henson WR, Moon TS
  TITLE     Molecular Toolkit for Gene Expression Control and Genome 
            Modification in Rhodococcus opacus PD630.
  JOURNAL   ACS Synth Biol. 2018 Feb 16;7(2):727-738. doi: 
            10.1021/acssynbio.7b00416. Epub 2018 Feb 5.
  PUBMED    29366319
REFERENCE   2  (bases 1 to 495)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.7b00416"; journalName: "ACS Synth Biol"; date: 
            "2018-02-16- 16"; volume: "7"; issue: "2"; pages: "727-738"
COMMENT     Sequence Label: pDD108
FEATURES             Location/Qualifiers
     source          1..495
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gcagatacgt gtgctgatcc aacgcctgcg cactcatcac gaaccccttc cattcgaaag
       61 aatcgaaaca cttttcgaat ctttcgaata gctggttcaa tcaccgccgc ttacatggcg
      121 agatcgacca cgtcccacct gccgagtacg agaccgccta ttggtcgaat cacacggcgg
      181 cactacggtg agacaccggt cctcgctgag gccggaacca gataaccgag cctccgccga
      241 actcggggca ggtgtcaccg ccccggtcct cgagagcaag ctcgacggtg tcagcgaaat
      301 gcgtgaacac ctcagccgcg aactggccca gcagcccacc cgccgcatcc ctatccggct
      361 cagcgacgaa tgcacccgcg ccaacaccgc cgcccgcaca gccagccaga acctcgaaac
      421 ctgaatccgc gaatgtgtgc ggtcgccgcc gcagtgctcg ggcattccgg ccgaaccgtt
      481 gtgcctgtct caatc
//
