LOCUS       sequence-227272-         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pNG2 backbone with an optimized chloramphenicol resitance marker for
            Rhodococcus opacus PD630.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  AUTHORS   DeLorenzo DM, Rottinghaus AG, Henson WR, Moon TS
  TITLE     Molecular Toolkit for Gene Expression Control and Genome 
            Modification in Rhodococcus opacus PD630.
  JOURNAL   ACS Synth Biol. 2018 Feb 16;7(2):727-738. doi: 
            10.1021/acssynbio.7b00416. Epub 2018 Feb 5.
  PUBMED    29366319
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.7b00416"; journalName: "ACS Synth Biol"; date: 
            "2018-02-16- 16"; volume: "7"; issue: "2"; pages: "727-738"
COMMENT     Sequence Label: pDD112
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      782..853
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 tgtgcgggct ctaacacgtc ctagtatggt aggatgagca acatttcgac gccgagagat
       61 tcgccgcccg aaatgagcac gatccgcatg cttaattaag aaggagatat acatatggag
      121 aaaaagatca cggggtacac caccgtggac atcagccagt ggcaccgtaa ggaacatttc
      181 gaagcgttcc agtccgtcgc gcaatgtacg tacaatcaga ccgtccaact cgatattacg
      241 gcgtttctga agaccgtgaa gaagaataag cacaaatttt accccgcctt tattcatatc
      301 ctggcgcggc tgatgaacgc ccatccagaa tttcgcatgg ccatgaaaga tggcgagttg
      361 gtcatctggg atagcgtcca tccatgttat acggtgttcc acgagcagac cgaaaccttt
      421 tcgagcttgt ggagcgagta tcacgatgat ttccggcagt tcttgcacat ttatagccaa
      481 gatgtggcct gctatggtga gaatctggcc tactttccga aaggtttcat tgagaacatg
      541 ttttttgtgt ccgccaatcc gtgggtctcg ttcacgagct tcgacctcaa cgtcgcgaat
      601 atggataact tttttgcgcc ggtctttacc atggggaaat attatacgca gggcgataag
      661 gtgctgatgc cactcgcgat ccaagtgcat catgccgtct gcgatggctt ccacgtcggt
      721 cggatgttga acgaactcca acaatattgc gatgagtggc aaggtggtgc gtaagctatg
      781 ccaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
      841 ggtgaacgct ctc
//
