LOCUS       sequence-227274-        3366 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Arabinose inducible dCas9sth1 and constitutive sgRNA on pAL5000/pMB1
            backbone with gentamicin resistance..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3366)
  AUTHORS   DeLorenzo DM, Rottinghaus AG, Henson WR, Moon TS
  TITLE     Molecular Toolkit for Gene Expression Control and Genome 
            Modification in Rhodococcus opacus PD630.
  JOURNAL   ACS Synth Biol. 2018 Feb 16;7(2):727-738. doi: 
            10.1021/acssynbio.7b00416. Epub 2018 Feb 5.
  PUBMED    29366319
REFERENCE   2  (bases 1 to 3366)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.7b00416"; journalName: "ACS Synth Biol"; date: 
            "2018-02-16- 16"; volume: "7"; issue: "2"; pages: "727-738"
COMMENT     Sequence Label: pDD143
FEATURES             Location/Qualifiers
     source          1..3366
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgtcggacc tggtgctggg cctggccatc ggcatcggct cggtgggcgt gggcatcctg
       61 aacaaggtga ccggcgagat catccacaag aactcgcgca tcttcccggc cgcccaggcc
      121 gagaacaacc tggtgcgccg caccaaccgc cagggccgcc gcctggcccg ccgcaagaag
      181 caccgccgcg tgcgcctgaa ccgcctgttc gaggagtcgg gcctgatcac cgacttcacc
      241 aagatctcga tcaacctgaa cccgtaccag ctgcgcgtga agggcctgac cgacgagctg
      301 tcgaacgagg agctgttcat cgccctgaag aacatggtga agcaccgcgg catctcgtac
      361 ctggacgacg cctcggacga cggcaactcg tcggtgggcg actacgccca gatcgtgaag
      421 gagaactcga agcagctgga gaccaagacc ccgggccaga tccagctgga gcgctaccag
      481 acctacggcc agctgcgcgg cgacttcacc gtggagaagg acggcaagaa gcaccgcctg
      541 atcaacgtgt tcccgacctc ggcctaccgc tcggaggccc tgcgcatcct gcagacccag
      601 caggagttca acccgcagat caccgacgag ttcatcaacc gctacctgga gatcctgacc
      661 ggcaagcgca agtactacca cggcccgggc aacgagaagt cgcgcaccga ctacggccgc
      721 taccgcacct cgggcgagac cctggacaac atcttcggca tcctgatcgg caagtgcacc
      781 ttctacccgg acgagttccg cgccgccaag gcctcgtaca ccgcccagga gttcaacctg
      841 ctgaacgacc tgaacaacct gaccgtgccg accgagacca agaagctgtc gaaggagcag
      901 aagaaccaga tcatcaacta cgtgaagaac gagaaggcca tgggcccggc caagctgttc
      961 aagtacatcg ccaagctgct gtcgtgcgac gtggccgaca tcaagggcta ccgcatcgac
     1021 aagtcgggca aggccgagat ccacaccttc gaggcctacc gcaagatgaa gaccctggag
     1081 accctggaca tcgagcagat ggaccgcgag accctggaca agctggccta cgtgctgacc
     1141 ctgaacaccg agcgcgaggg catccaggag gccctggagc acgagttcgc cgacggctcg
     1201 ttctcgcaga agcaggtgga cgagctggtg cagttccgca aggccaactc gtcgatcttc
     1261 ggcaagggct ggcacaactt ctcggtgaag ctgatgatgg agctgatccc ggagctgtac
     1321 gagacctcgg aggagcagat gaccatcctg acccgcctgg gcaagcagaa gaccacctcg
     1381 tcgtcgaaca agaccaagta catcgacgag aagctgctga ccgaggagat ctacaacccg
     1441 gtggtggcca agtcggtgcg ccaggccatc aagatcgtga acgccgccat caaggagtac
     1501 ggcgacttcg acaacatcgt gatcgagatg gcccgcgaga ccaacgagga cgacgagaag
     1561 aaggccatcc agaagatcca gaaggccaac aaggacgaga aggacgccgc catgctgaag
     1621 gccgccaacc agtacaacgg caaggccgag ctgccgcact cggtgttcca cggccacaag
     1681 cagctggcca ccaagatccg cctgtggcac cagcagggcg agcgctgcct gtacaccggc
     1741 aagaccatct cgatccacga cctgatcaac aactcgaacc agttcgaggt ggacgccatc
     1801 ctgccgctgt cgatcacctt cgacgactcg ctggccaaca aggtgctggt gtacgccacc
     1861 gccaaccagg agaagggcca gcgcaccccg taccaggccc tggactcgat ggacgacgcc
     1921 tggtcgttcc gcgagctgaa ggccttcgtg cgcgagtcga agaccctgtc gaacaagaag
     1981 aaggagtacc tgctgaccga ggaggacatc tcgaagttcg acgtgcgcaa gaagttcatc
     2041 gagcgcaacc tggtggacac ccgctacgcc tcgcgcgtgg tgctgaacgc cctgcaggag
     2101 cacttccgcg cccacaagat cgacaccaag gtgtcggtgg tgcgcggcca gttcacctcg
     2161 cagctgcgcc gccactgggg catcgagaag acccgcgaca cctaccacca ccacgccgtg
     2221 gacgccctga tcatcgccgc ctcgtcgcag ctgaacctgt ggaagaagca gaagaacacc
     2281 ctggtgtcgt actcggagga ccagctgctg gacatcgaga ccggcgagct gatctcggac
     2341 gacgagtaca aggagtcggt gttcaaggcc ccgtaccagc acttcgtgga caccctgaag
     2401 tcgaaggagt tcgaggactc gatcctgttc tcgtaccagg tggactcgaa gttcaaccgc
     2461 aagatctcgg acgccaccat ctacgccacc cgccaggcca aggtgggcaa ggacaaggcc
     2521 gacgagacct acgtgctggg caagatcaag gacatctaca cccaggacgg ctacgacgcc
     2581 ttcatgaaga tctacaagaa ggacaagtcg aagttcctga tgtaccgcca cgacccgcag
     2641 accttcgaga aggtgatcga gccgatcctg gagaactacc cgaacaagca gatcaacgac
     2701 aagggcaagg aggtgccgtg caacccgttc ctgaagtaca aggaggagca cggctacatc
     2761 cgcaagtact cgaagaaggg caacggcccg gagatcaagt cgctgaagta ctacgactcg
     2821 aagctgggca accacatcga catcaccccg aaggactcga acaacaaggt ggtgctgcag
     2881 tcggtgtcgc cgtggcgcgc cgacgtgtac ttcaacaaga ccaccggcaa gtacgagatc
     2941 ctgggcctga agtacgccga cctgcagttc gacaagggca ccggcaccta caagatctcg
     3001 caggagaagt acaacgacat caagaagaag gagggcgtgg actcggactc ggagttcaag
     3061 ttcaccctgt acaagaacga cctgctgctg gtgaaggaca ccgagaccaa ggagcagcag
     3121 ctgttccgct tcctgtcgcg caccatgccg aagcagaagc actacgtgga gctgaagccg
     3181 tacgacaagc agaagttcga gggcggcgag gccctgatca aggtgctggg caacgtggcc
     3241 aactcgggcc agtgcaagaa gggcctgggc aagtcgaaca tctcgatcta caaggtgcgc
     3301 accgacgtgc tgggcaacca gcacatcatc aagaacgagg gcgacaagcc gaagctggac
     3361 ttctaa
//
