LOCUS       sequence-227275-         204 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Arabinose inducible dCas9sth1 and constitutive sgRNA on pAL5000/pMB1
            backbone with gentamicin resistance..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 204)
  AUTHORS   DeLorenzo DM, Rottinghaus AG, Henson WR, Moon TS
  TITLE     Molecular Toolkit for Gene Expression Control and Genome 
            Modification in Rhodococcus opacus PD630.
  JOURNAL   ACS Synth Biol. 2018 Feb 16;7(2):727-738. doi: 
            10.1021/acssynbio.7b00416. Epub 2018 Feb 5.
  PUBMED    29366319
REFERENCE   2  (bases 1 to 204)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.7b00416"; journalName: "ACS Synth Biol"; date: 
            "2018-02-16- 16"; volume: "7"; issue: "2"; pages: "727-738"
COMMENT     Sequence Label: pDD143
FEATURES             Location/Qualifiers
     source          1..204
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gtttttgtac tcgaaagaag ctacaaagat aaggcttcat gccgaaatca acaccctgtc
       61 attttatggc agggtgtttt ttttttgtcg acttggggac cctagaggtc ccctttttta
      121 tttttttgga tgaagagcat tatcaatggc tcttcacagg aagcttaaaa aaaaaagccc
      181 cgcgattgcg gggctttttt tttt
//
